http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Duchenne 근이영양증 환자에서 Propofol과 Remifentanil을 이용한 전정맥마취 경험 : 증례보고
김상훈,이현영,소금영,정종달,고지현 朝鮮大學校 附設 醫學硏究所 2009 The Medical Journal of Chosun University Vol.33 No.S
Patients with Duchenne's muscular dystrophy are considered to be at risk of peri-operative complications. It's patients can be associated with cardiac arrest, rhabdomyolysis, malignant hyperthermia during anaesthesia. This is a case report of a 14 years old male patient who had known Duchenne muscular dystrophy was scheduled for open reduction and internal fixation of right humerus fracture. Regional anesthesia was refused by the patient. Total intravenous anesthesia (TIVA) was performed with propofol and remifentanil, which was started at the targeted effect concentration of 3.0μg/ml and 10ng/ml via target controlled infusion. After the end of operation, the patient was awaken without any problem and was transferred to recovery room.
김지호,류권일,고영헌,김팔규 忠南大學校 産業技術硏究所 2001 산업기술연구논문집 Vol.16 No.2
In general, the term soft ground includes clayey soils, which have large compressibility and small shear resistance due to the external load. All process of consolidation in compressible soils can be explained in terms of transfer of load from an incompressible pore-water to a compressible soil structure. Therefore, one of the most important subjects about the characteristics of the time-dependant consolidation of the clay foundation by the charge of load may be the presumption of the final settlement caused by consolidation and the degree of consolidation according to the time. Pioneering work by Terzaghi imparted scientific and mathematical bases to many aspects of this subject and many people have used this theory to measure the consolidation settlement until now. In the paper, Finite Difference Methods for consolidation are considered. First, it is shown the stability criterion of Explicit scheme and the Crank-Nicolson scheme, although unconditionally stable in the mathematical sense, produces physically unrealistic solutions when the time step is large, it is also shown that The fully Implicit scheme shows more satisfactory behavior, but is less accurate for small time steps. And then we need to decide what scheme is more proper to consolidation. The purpose of this paper is to suggest the pertinent scheme to consolidation.
Effect of cell-density on in-vitro dopaminergic differentiation of mesencephalic precursor cells
Ko, Ji-Yun,Lee, Ji-Yeon,Park, Chang-Hwan,Lee, Sang-Hun Lippincott Williams Wilkins, Inc. 2005 NEUROREPORT - Vol.16 No.5
Neural precursor cells isolated from early embryonic mesencephalon are in-vitro expanded and differentiated toward dopamine neurons. However, conditions for controlled conversion of the precursors into dopamine neurons largely remained to be determined. We here examined the effects of plating cell density and duration of in-vitro cell expansion on the precursors-derived dopamine differentiation. The yield of dopamine neurons from cultured mesencephalic precursors was greater when the cells were initially plated at higher density. Soluble factors secreted from the precursors appeared to be responsible for the cell density effect. We further demonstrated that the dopamine differentiation potential of the precursors was lost after a long-term cell expansion. Therefore, in order to attain high percentage of dopamine neuron population in mesencephalic precursor cultures, cultures need to be seeded at high cell density and to be expanded for a short period of time.
Choi, Ji Hun,Ji, Young Geon,Ko, Jung Jae,Cho, Han Jun,Lee, Dong Hyeon Wolters Kluwer Health, Inc. All rights reserved. 2018 Pancreas Vol.47 No.5
OBJECTIVES: The aim of this study was to investigate the effects of the activated P2X7 receptors on the proliferation and growth of human pancreatic cancer cells. METHODS: Proliferation was measured by incorporating bromodeoxyuridine into pancreatic cancer cells, MIA PaCa-2 and HPAC. Expression of P2 receptors and signal molecules was examined using quantitative reverse transcription/polymerase chain reaction and/or Western blot. Proliferative effects of the P2X7 receptors in vivo were examined using a xenotransplant model of pancreatic cancer cell lines. RESULTS: Incubating pancreatic cancer cells with adenosine triphosphate (ATP) and 2&vprime;(3&vprime;)-O-(4-Benzoylbenzoyl)ATP resulted in a dose-dependent increase of cell proliferation. The P2 receptor antagonist, KN-62, and small interfering RNA against P2X7 receptors, significantly decreased the proliferative effects of ATP. The ATP-induced proliferation was mediated by protein kinase C, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2), and c-Jun N-terminal kinase (JNK); specifically, ATP increased the phosphorylation of ERK1/2 and JNK. The expression of inducible nitric oxide synthase was decreased by P2X7 receptor activation. In a xenotransplant model, applying ATP significantly increased the growth of induced tumors. CONCLUSIONS: The P2X7 receptor activation by extracellular nucleotides increased proliferation and growth of human pancreatic cancer cells via ERK1/2 and JNK. This supports the pathophysiological role of P2X7 receptors in pancreatic disease and recovery.
Ko, Kwangseok,Kim, Hye-Jung,Ho, Pil-Su,Lee, Soon Ok,Lee, Ji-Eun,Min, Cho-Rong,Kim, Yu Chul,Yoon, Ju-Han,Park, Eun-Jung,Kwon, Young-Jin,Yun, Jee-Hun,Yoon, Dong-Oh,Kim, Jung-Sook,Park, Woul-Seong,Oh, Se American Chemical Society 2018 Journal of medicinal chemistry Vol.61 No.7
<P>The histamine H4 receptor (H4R), a member of the G-protein coupled receptor family, has been considered as a potential therapeutic target for treating atopic dermatitis (AD). A large number of H4R antagonists have been disclosed, but no efficient agents controlling both pruritus and inflammation in AD have been developed yet. Here, we have discovered a novel class of orally available H4R antagonists showing strong anti-itching and anti-inflammation activity as well as excellent selectivity against off-targets. A pharmacophore-based virtual screening system constructed in-house successfully identified initial hit compound <B>9</B>, and the subsequent homology model-guided optimization efficiently led us to discover pyrido[2,3-<I>e</I>]tetrazolo[1,5-<I>a</I>]pyrazine analogue <B>48</B> as a novel chemotype of a potent and highly selective H4R antagonist. Importantly, orally administered compound <B>48</B> exhibits remarkable efficacy on antipruritus and anti-inflammation with a favorable pharmacokinetic (PK) profile in several mouse models of AD. Thus, these data strongly suggest that our compound <B>48</B> is a promising clinical candidate for treatment of AD.</P> [FIG OMISSION]</BR>
Ko, Ji-Yun,Lee, Hyun-Seob,Park, Chang-Hwan,Koh, Hyun-Chul,Lee, Yong-Sung,Lee, Sang-Hun Academic Press 2009 MOLECULAR THERAPY Vol.17 No.10
<P>We have previously demonstrated derivation of neural precursor (NP) cells of a midbrain-type from human embryonic stem (hES) cells to yield an enriched population of dopamine (DA) neurons. These hES-derived NPs can be expanded in vitro through multiple passages without altering their DA neurogenic potential. Here, we studied two aspects of these hES-NP cells that are critical issues in cell therapeutic approaches for Parkinson's disease (PD): cell survival and tumorigenic potential. Neuroepithelial rosettes, a potentially tumorigenic structure, disappeared during hES-NP cell expansion in vitro. Although a minor population of cells positive for Oct3/4, a marker specific for undifferentiated hES cells, persisted in culture during hES-NP cell expansion, they could be completely eliminated by subculturing hES-NPs under differentiation-inducing conditions. Consistently, no tumors/teratomas are formed in rats grafted with multipassaged hES-NPs. However, extensively expanded hES-NP cells easily underwent cell death during differentiation in vitro and after transplantation in vivo. Transgenic expression of Bcl-XL and sonic hedgehog (SHH) completely overcame the cell survival problems without increasing tumor formation. These findings indicate that hES-NP cell expansion in conjunction with Bcl-XL+SHH transgene expression may provide a renewable and safe source of DA neurons for transplantation in PD.</P>