미세결정성 셀룰로오스에 Trichoderma viride 셀룰라아제의 흡착거동

오제직,이혜옥 공주대학교 사범대학 과학교육연구소 1992 과학교육연구 Vol.23 No.1

표면적의 크기가 다른 미세결정성 셀룰로오스에 대한 Trichoderma viride cellulase의 흡착특성을 알아보았다. 셀룰로오스에 대한 셀룰라아제의 최적츱착 pH는 4.8이었다. 셀룰라아제의 흡착은 5℃ 또는 그보다 낮은 온도에서 가장 높았으며, 반응온도가 높아짐에 따라 흡착량이 감소하였다. 그러나 고유흡착량은(흡악된 효소활성/mg 단백질) 셀룰로오스의 최적 가수분해 조건인 5℃ 그리고 pH 4.8에서 가장 높았다. 셀룰로오스에 대한 셀룰라아제의 흡착은 랭뮤어 등온식에 따라 흡착을 하였다. 셀룰로오스의 표면적이 커짐이 따라 최대흡적량(Amax)이 증가하였고, 온도가 높아짐에 따라 최대흡착량(Amax)과 흡착상수(K)의 값이 감소하였다. 셀룰로오스에 대한 셀룰라아제의 흡착 열역학적파라미터는 흡착상수로부터 계산하였다. 셀룰로오스에 대한 셀룰라아제의 흡착시, 큰 음의 흡착 자유에너지의 값을 가졌고, 이로부터 흡착은 자발적으로 진행됨을 알 수 있었다. 이 흡착반응은 발열반응이고, 엔탈피지배반응이다. The adsorption behaviors of cellulase components of Trichoderma viride on microcrystalline cellulose with different surface area were studied. The optimum pH of adsorption of cellulase components on microcrystalline cellulose exhibited at 4.8. The adsorption of cellulase components was highest at temperature of 5℃ or lower and decreased with increasing the temperature. But, specific adsorption of cellulase components, activity adsorbed/mg protein, was found to be maximum at the optimal hydrolysis conditions of 50℃ and pH 4.8. The adsorption of cellulase components on microcrystalline cellulose obeys the Langmuir absorption isotherm. There was an increase in maximum adsorption amount(Amax) as the surface area of microcrystalline cellulose is increased. The value of maximum adsorption amount(Amax) and adsorption equilibrium constant(K) decreased with increasing the temperature. Thermodynamic parameters in adsorption were calculated from the adsorption equilibrium constant. It was found that from the large negative value of the free energy changes of the adsorption of cellulase components on microcrystalline cellulose can take place spontaneously. The adsorption process was exothermic and adsorption enthalpy controlled reaction.

중학교 3학년 과학교과서 내용의 비교 연구

오제직,임갑수 공주대학교 사범대학 과학교육연구소 1992 과학교육연구 Vol.23 No.1

The contents of five different science textbooks presently in use and the older science textbook(published before 1987) were selected and analyzed. More active reforms in science textbooks are necessary for future revision to fit the needs of science education in the 21st century. The results of this study can be summarized as follows: 1. There was little difference in the quantity of materials among five kinds of science textbooks now in use. 2. Science textbooks now in use had more pages, reading, nd Gothic-type terms than old science textbook. 3. Science textbooks now in use have less figures and tables than old science textbook. 4. Each of the science textbooks now in use had so many common parts that its contents were almost the same. 5. It was found that some terms used in the five kinds of science textbooks now in use were not unified. 6. It was found that some of the materials in five kinds of science textbooks now in use varied in thier contents.

현행 고등학교 화학 교과서 비교 연구

오제직,최석남,이종길 공주대학교 사범대학 과학교육연구소 1994 과학교육연구 Vol.25 No.1

This paper aims to help the teachers select the textbook and provide them with the basic teaching materials as an ideal model by analyzing the organization (its element and appropriateness), volume amount, experiment matter, and the contents of the highschool chemistry textbooks, which have been revised in accordance with the 5th amended curriculum. The results of the analogy are as follow 1. Organization (its element and appropriateness) : Though having some distincitive characteristics, all the textbooks have almost the same introduction, development, summary, and further study, which are appropriate for the textbook model. 2. The volume amount: All the textbooks have almost the same frame, but s a big difference in such things as the number of pages, of pictures and charts, of Gothic terminology, and relative importance of each chapter. And all the materials such as pictures and photos are black-and-white, which we think are less helpful to the students` study. 3. The experiment matter: There`s a geat difference in the number, kinds, ratio contents and subject of experiment in each chapter. And there are some distinctive characteristics in the type, level, method, and investigative skills of the experiments. 4. The contents : There are considerable differences in the additional and reduced sections. Therefore, which book is selected is very important, and the teachers should not skip any class-work.

고등학교 과학(Ⅱ) 교과서 내용의 비교·분석 연구

오제직,홍춘표,김화영 공주대학교 사범대학 과학교육연구소 1994 과학교육연구 Vol.25 No.1

The purpose of this research is to compare and to analyze eight different science textbooks Ⅱ (the last volume) that are presently in use at the high school. The items that were compared and analrzed are as follows: The construction of each textbook in quantity. The construction of experimentation contents. The construction of contents in textbooks. This study will provide the basic data that is needed to effectively accomplish aims of science education and to enable a guidance teacher to reconstruct the textbook properly to the real condition exiting in each school. The findings of the research are as follows: 1. There is little difference in quantity of materials. But there is difference in quantity of each unit. 2. The number of Gothic-type terms used in each textbook is not unified. The sort of Gothic-type terms used in each textbook is also varied. 3. Photographs and pictures are presented in black and white. They are used for only explaining. 4. Material and method used in experimentation are showed in many ways. But some units are given too much importance. 5. Contents are consisted variously according to authors under the basic frame of education curriculum.

??-Myristoyl-L-Cysteine에 의한 p-Nitrophenyl Acetate의 가수분해에 관한 연구

吳濟直,洪春杓 공주대학교 사범대학 과학교육연구소 1986 과학교육연구 Vol.18 No.1

The hydrolysis of p-nitrophenyl acetate has been studied aquous buffer solution in the presence of mixed micelles. The pseudo-first-order rate constant for PNPA has been calculated in the presence ??-myristoyl-L-cysteine varied concentration, ??, ??, ?? and ?? and its were ??, ??, ?? and ??. A remarkably large raate enchancement in the hydrolysis of PNPA was obs-erved in the mixed micelles. The recond-order rate constants were calculated each 1.2×10², 4.1×10², 8.0×10²and 8.3×10²at PH 5.0, 6.0, 7.2 and 8.0.

제6차 교육과정에 의한 중학교 과학 교과서의 비교 분석 연구 : 중학교 1학년 과학 교과서를 중심으로 On the First Year of Middle School

오제직,홍춘표,김준식 공주대학교 사범대학 과학교육연구소 1996 과학교육연구 Vol.27 No.1

The purpose of this paper is to give basic data which could be used for re-construction textbooks according to real situation of school in teaching, and could help to write new textbooks. For this study, five kinds of Science textbooks Ⅰ on the 5th curriculum and eight kinds of science textbooks Ⅰ on the 6th curriculum were selected as an analytical material. The methods of study are as follows; We analyzed and compared respectively with Githic type, Figure, Table, Research, Question, Experiment, Observation, Exercise and so forth in eight kinds of Sience textbooks Ⅰ and in average values of five kinds of textbook on the 5th curriculum. 1. The kinds of the Science textbook based on the 6th curriculum and 5th curriculum were respectively 8 and 5. 2. The quantity of new textbooks were much the same as the old textbooks, except for C and H textbooks. 3. It was found out that new textbooks decreased more than old textbooks in number of page of theory and explanation. 4. The number of Gothic type term was showed more Chapter Ⅰ and Ⅱthan other chapter in old and new science textbooks. 5. Figure and Table have intrinsic character-respectively. 6. The number of Study and Figure were unchanged as compared with old textbooks. but its of Table were decreased. 7. The number of Question was also unchanged, but it had almost subjective. 8. The kinds and numbers of experiments were increased as compared with old textbooks, and they had instinctive character respectively. From these analytical results of new science textbooks, we, that is, science teachers should select science textbook well and we should supply a thing of insufficiecy on science textbook in teaching.

中學校 科學 敎科書의 比較分析 : 中學校 Ⅱ學年 科學 敎科書를 中心으로

洪春杓,吳濟植,金鎭彦 공주대학교 사범대학 과학교육연구소 1990 과학교육연구 Vol.22 No.1

Recently science curriculum in middle school was reformed the fifth and the science textbook of middle school is five species. In order to prompte educational effect, this study compared and analyzed five kinds of new science textbook with an old science textbook of the second year in middle school The results of this study can be summarized as follows. 1) Reformed five kinds of science textbook have various contents by participating 50 authors 2) Reformed science textbook increased 10 pages more than old science textbook of middle school in quantity. 3) It was found out that the numbers of the "Study and Observation" and "Exercises and Questions" in new science textbooks increased. 4) The numbers of Pictures were unchanged and the numbers of Tables. Experiments, Exercises and Composite Questions were decreased. 5) The 38∼42% was appeared the ratio of Experiments, Tables, Pictures, and Goethic type Terms in more than three kinds of science textbooks. 6) New science textbooks were reflected 61∼75% of an old science textbooks in Pictures, Goethic type Terms, Tables, and Experiments. As these results Terms and Contents reprented by Goethic type in science textbook should be selected carefully in order to prompte educational effect. The selection of Exercises, Composite Questions, Experiments and Observations should be allocated equally to each unit and behavior area. And this study suggests that there should be introduced experiments of an environment pollusion and its influence into a science textbook.

휴대용 계산기에 의한 착물의 안정도상수의 계산

윤세중,오제직,박병빈,박성록 공주대학교 사범대학 과학교육연구소 1980 과학교육연구 Vol.12 No.1

A Pocket calcultor program(Texas Instruments TI58 or TI59) was constructed for evaluation of the Successive stability constants of Nikel-glycine complex. This program conld be used for many other systems having similar eguhibrium scheme and experimental method. TI59, this program could be extended to the calculation of ionic strength correction and high n Complexes.

다공질 실리콘 카바이드의 제작 및 광발광 성질

이기환,오제직,최석남,박병빈,이태호,김영유 공주대학교 사범대학 과학교육연구소 1998 과학교육연구 Vol.29 No.1

p-type α-SiC(4H, 6H, 15R polytype)를 사용하여 다공질 실리콘(porous silicon; PS)의 제작방법과 유사하게 HF/H₂O/C₂H??OH 용액에서 전기화학적으로 에칭을 하여 광발광 특성을 가지는 다공질 실리콘 카바이드(porous silicon carbide; PSC)를 제작하였다. PSC의 다공질의 분포는 전류밀도가 증가할수록 증가하고 다공질의 크기는 감소하였으며 광발광(photoluminescence; PL) 스펙트럼의 분석을 통해 전극 표면에서의 산화반응은 공급되는 전자의 양과 전류밀도에 의존하며 전류밀도가 증가할수록 전극의 산화반응은 억제된다는 결과를 얻었다. Porous silicon carbide(PSC) having photoluminescence(PL) can be prepared by a technique very similar to that used in porous silicon preparation, by electrochemical etching of crystalline p-type α-SiC(4H, 6H, 15R polytype) in ?? solution. The results show that porous distribution increase with increasing of the current densities while pore size decrease with that. Through the PL spectra analysis anodic oxidation of electrode surface was dependent on the amount of provided electrons and the current densities and inhibited with increasing of the current densities.

실리콘 내열 도료의 개발에 관한 연구

서정목,류해일,홍춘표,오제직 公州大學校工科大學生産技術硏究所 1996 論文集 Vol.4 No.-

Nowdays, the development of industrial tequnique needs newly specialized materials which are stable under the chemical and physical conditions. The development of the paints which have heat-resistance at high temperature brings much profits to industries. Inorganic heat-resistance paints are used limitedly to coat special surface of machine by methods of CVD and PVD. These materials are goods of Pyrocat in Oodake Co. LTD., Neccera Coat in JCR Co. LTD., Pyromax in Aremco Co. LTD., and Ceratile in Elosol Co. LTD.. In this study, phtalocyanino silicon series and copolymer were prepared from phtalocyanine and corresponding silanes. The oligomeric materials were Dichloro-(phthalocyaninato)-silcon(PcSiCl₂), Dihydroxy-(phthalocyaninato)-silicon (PcSi(OH)₂), Dimethyldichlorosilane-Phthallocyaninato-silcon (MCS-PcSi) copolymer, Dimethyldichlorosilane-Hydroquinone (MCS-HQ) copolymer, Hydroquinone-Phthalocyanine (HQ- PcSi) copolymer, PcSi(O(CH₂)7CH₃)₂, PcSi(O(CH₂)9CH₃)₂, and PcSi (O(CH₂)11CH₃)₂. The oligomeric materals and other additives are ballmilled for about 16 hours and reduced to coating viscosity with xylene. And they were tested for coating membrane form, specific gravity, viscosity, pH, adhesion, pencil-hardness, chemicaland water-resistance, heat-resistance, impact resistance, and heat-resistance. Heat-resistance paints of phtalocyanino silicone series were developed, which are curing, 170℃, maximum operating temperature of 355℃, and maximum heat-resistant temperature of 560℃. The phtalocyanino silicone series paint which can be used in bonding agent and dye are expected to apply usefully in the industries.