백서 간재생중 H2B Histone 유전자 발현에 대한 Octamer Binding Transcription Factor의 역할

임규,방재웅,최두식,손미영,이영철,박청,김태동,김영래,박종일,윤완희,박승길,황병두 한국노화학회 2001 한국노화학회지 Vol.11 No.1

STUDIES FOR THE CHARACTER OF NANO-SIZED TiO₂ PARTICLE SYNTHESIZED BY MICRO-EMULSION METHOD AND GOLD-DEPOSITED TiO₂ PARTICLE

( Hyun-pyo Jhun ),( Jae-kiel Park ),( Kyoung-chul Lee ),( Jae-eon Park ) 대한화장품학회 1996 대한화장품학회지 Vol.22 No.2

Nano-Sized TiO₂ particles with diameter between 2 and 5 nm are synthesized in Water/ Triton X-100/ n-Hexane microemuelsion. Particles show the amorphous structure and partially hydroxide form. The optical absorbance of particles appears at 250nm and band edge at 340nm. Gold metal is deposited on the surface of TiO₂ particles by reduction reaction of Au(lll) ion with sodium hypophosphite. The size of gold-deposited particles is 20nm, and the optical absorbance appears at 270nm and at 550nm. So particles show the red color. The dense precipitation is formed by aggregation in the TiO₂ nano-sized particles of about 5nm size. But the bulky precipitation is formed by agglomeration phenomena in the gold- deposited particles of 20nm size. And also gold-deposited particles is easily dispersed by being re-dispersed in PEG/Water solution. This study has compared those things measuring the SPF characteristics of the cosmetics made of the synthesized particles. If the particle size is controlled appropriately, then the SPF value will be higher, or more colorless cosmetics will be made.

Production and Purification of Soluble Recombinant Human Lymphotoxin in Escherichia coli

Choi, Yoon Ho,Na, Doe Sun,Pan, Jae gu,Park, Seung Kiel,Yoo, Hyang Sook,Kang, Kook Hee,Sung, Moon Hee 한국미생물 · 생명공학회 1992 Journal of microbiology and biotechnology Vol.2 No.1

Human lymphotoxin (LT) was produced in E. coli as a soluble protein. The level of recombinant human LT production was about 4% of the total souble proteins of E. coli extracts. Recominant human LT was purified to apparant homogeneity by a simple procedure utilizing FPLC on Mono Q and Mono S columns. The specific activity of the purified LT was 1.0×10 exp (7) units/㎎.

Dexamethasone Induces FcgRIIb Expression in RBL-2H3 Cells

Prashanta Silwal,Mi-Nam Lee,Choong-Jae Lee,Jang-Hee Hong,Uk Namgung,Zee-Won Lee,Jinhyun Kim,Kyu Lim,Gi Ryang Kweon,Jong IL Park,Seung Kiel Park 대한생리학회-대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.6

Mast cells are involved in allergic responses, protection against pathogens and autoimmune diseases. Dexamethasone (Dex) and other glucocorticoids suppress FcՅRI-mediated release of inflammatory mediators from mast cells. The inhibition mechanisms were mainly investigated on the downstream signaling of Fc receptor activations. Here, we addressed the effects of Dex on Fc receptor expressions in rat mast cell line RBL-2H3. We measured mRNA levels of Fc receptors by real-time PCR. As expected, Dex decreased the mRNA levels of activating Fc receptor for IgE (FcՅR) I and increased the mRNA levels of the inhibitory Fc receptor for IgG FcՃRIIb. Interestingly, Dex stimulated transcriptions of other activating receptors such as Fc receptors for IgG (FcՃR) I and FcՃRIII. To investigate the mechanisms underlying transcriptional regulation, we employed a transcription inhibitor actinomycin D and a translation inhibitor cycloheximide. The inhibition of protein synthesis without Dex treatment enhanced FcՃRI and FcՃRIII mRNA levels potently, while FcՅRI and FcՃRIIb were minimally affected. Next, we examined expressions of the Fc receptors on cell surfaces by the flow cytometric method. Only FcՃRIIb protein expression was significantly enhanced by Dex treatment, while FcՃRI, FcՃRIII and FcՅRI expression levels were marginally changed. Our data showed, for the first time, that Dex regulates Fc receptor expressions resulting in augmentation of the inhibitory receptor FcՃRIIb.

Dexamethasone Induces $Fc{\gamma}RIIb$ Expression in RBL-2H3 Cells

Silwal, Prashanta,Lee, Mi-Nam,Lee, Choong-Jae,Hong, Jang-Hee,NamGung, Uk,Lee, Zee-Won,Kim, Jinhyun,Lim, Kyu,Kweon, Gi Ryang,Park, Jong Il,Park, Seung Kiel The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.6

Mast cells are involved in allergic responses, protection against pathogens and autoimmune diseases. Dexamethasone (Dex) and other glucocorticoids suppress $Fc{\varepsilon}RI$-mediated release of inflammatory mediators from mast cells. The inhibition mechanisms were mainly investigated on the downstream signaling of Fc receptor activations. Here, we addressed the effects of Dex on Fc receptor expressions in rat mast cell line RBL-2H3. We measured mRNA levels of Fc receptors by real-time PCR. As expected, Dex decreased the mRNA levels of activating Fc receptor for IgE ($Fc{\varepsilon}R$) I and increased the mRNA levels of the inhibitory Fc receptor for IgG $Fc{\gamma}RIIb$. Interestingly, Dex stimulated transcriptions of other activating receptors such as Fc receptors for IgG ($Fc{\gamma}R$) I and $Fc{\gamma}RIII$. To investigate the mechanisms underlying transcriptional regulation, we employed a transcription inhibitor actinomycin D and a translation inhibitor cycloheximide. The inhibition of protein synthesis without Dex treatment enhanced $Fc{\gamma}RI$ and $Fc{\gamma}RIII$ mRNA levels potently, while $Fc{\varepsilon}RI$ and $Fc{\gamma}RIIb$ were minimally affected. Next, we examined expressions of the Fc receptors on cell surfaces by the flow cytometric method. Only $Fc{\gamma}RIIb$ protein expression was significantly enhanced by Dex treatment, while $Fc{\gamma}RI$, $Fc{\gamma}RIII$ and $Fc{\varepsilon}RI$ expression levels were marginally changed. Our data showed, for the first time, that Dex regulates Fc receptor expressions resulting in augmentation of the inhibitory receptor $Fc{\gamma}RIIb$.

POSTERS / PM-025 : PHOSPHORYLATION - DEPENDENT REGULATION MECHANISM OF PHOSPHOLIPASE D1 BY PROTEIN KINASE C IN CELLS

(Yong Kim),(Dong Mei Zhao),(Jong Bae Park),(Sang Do Lee),(Yong Seok Oh),(Churo Chung),(Tae Hoon Lee),(Jae Ho Kim),(Seung Kiel Park),(Jong Shin Yoo) 한국생화학분자생물학회 (구 한국생화학회) 1999 6th IUBMB SEOUL CONFERENCE Vol.- No.-

O/W 에멀젼 입자의 크기에 미치는 Direct 유화법과 Invert유화법의 효과

김철훈 ( Cheol-hun Kim ),박재길 ( Jae-kiel Park ),황정웅 ( Jeong-wung Hwang ),허정림 ( Jung-rim Haw ) 대한화장품학회 1994 대한화장품학회지 Vol.20 No.1

유화제로 polyoxyethylene(20)sorbitan monostearate, sorbitan sesquioleate을 혼합 사용하고, 분산상으로 liquid paraffin, 연속상으로 deionized water를 원료로 사용하여 Direct유화법을 이용한 전상법과 Invert유화법을 3가지 단계의 제조방법으로 나누어서 O/W에멀젼을 제조하였고, 각 단계 전상유화법에서 첨가되는 물의 양에 따른 최종 O/W에멀젼의 입자크기변화를 관찰하였다. Direct유화법을 이용한 2단계 전상유화법을 사용하여 제조한 결과, 미세하고 균일한 입자를 갖는 O/W에멀젼은 W/O에서 (W/O)W 이중에멀젼으로의 전환 단계를 거쳐야만 생성됨을 알 수 있었다. Invert유화법을 사용한 3단계전상유화법은 W/O 에서 일종의 Pseudomicroemulsion gel상을 거쳐 미세하고 균일한 O/W유화 입자가 얻어졌다. 3단계 전상법이 2단계 전상법보다 더 미세하고 균일한 입자를 생성하였다. 실험에서 사용한 모든 유화법에서 혼합유화제의 HLB가 10.0 - 10.8 영역에서 가장 미세한 에멀젼입자가 형성되었다. 이 HLB영역에서 혼합유화제가 용해되어있는 오일상에 첨가되어 가용화되는 물의 양이 최대로 많았으며, 최대 가용화 물양을 갖는 유화제의 조성에서 가장 미세하고 균일한 입자가 얻어졌다. O/W emulsions with mixed nonionic surfactants(polyoxyethylene(20)sorbitan monostearate/sorbitan sesquioleate), liquid paraffin, water prepared by direct inversion emulisification method and continuous inversion emulsification Method. The one-step, two-step and three-step phase inversion emulsification method were used in experiments. Effect of added water on droplet size of final O/W emulsions which is prepared by phase inversion emulsification method were investigated. In direct inversion emulsification method(two-step emulsification method), fine and homogeneous droplets of OIW emulsions were formed after phase inversion steps i.e. ,W/O - (W/O) If double emulsion - O/W emulsion. In continuous inversion emulsification method(three-step emulsification method), fine and homogeneous O/W emulsion were formed after phase inversion steps i.e., W/O - pseudomicroemulsion - O/W. By latter method, more Fine and homogenuous droplets were formed than former method. 10-10, 8 HLB region of mixed non-ionic surfactants could produce most fine droplets. This HLB region had maximum values of solubilization water and This HLB value of mixed nonionic surfactants produced fine and homogenuous droplets.

O/W 에멀젼의 입자크기 및 상거동에 미치는 다양한 전상유화법의 효과

김철훈,박재길,허정림,유영태,박태욱,이치규 ( Cheol Hun Kim,Jae Kiel Park,Jung Rim Haw,Young Tai Yoo,Tae Wook Park,Chi Giu Lee ) 한국공업화학회 1995 공업화학 Vol.6 No.4

유화제는 polyoxyethylene(20)sorbitan monostearate와 sorbitan sesquioleate를 혼합 사용하고, 오일상으로는 liquid paraffin을 원료로 사용하여 다양한 전상유화 법으로 O/W 에멀젼을 제조하였다. 전상유화공정중에 오일상에 첨가되는 적량의 물은 가용화 되거나 유화되어 W/O 에멀젼으로 된다. 이 W/O 에멀젼은 분산상의 부피변화나(catastrophic inversion) 유화제의 오일과 물에 대한 친화도변화(transitional inversion)에 의해 O/W 에멀젼으로 전환된다. Direct 유화방법을 채택한 2단계 전상유화법의 공정에서는 잠정적인 (W/O)/W 이중에멀젼이 형성되었다 : 최종에멀젼이 W/O→(W/O)/W→O/W의 전환 단계를 거쳐서 생성되었다. 이 전환에서는 catastrophic inversion이 일어났다. 1단계 및 3단계 전상유화법은 W/O에서 pseudomicroemulsion gel상을 거쳐서 미세하고 균일한 O/W에멀젼입자가 얻어졌다 : W/O→pseudomicroem ulsion gel상→O/W. 이 전환에서는 연속상의 일정농도(5∼17wt%) 내에서 transitional inversion이 일어남을 알 수 있었고, 3단계 전상유화법이 2단계 전상유화법보다 더 미세하고 균일한 입자를 갖는 높은 점도의 에멀젼을 생성하였다. 본 연구에서 가장 미세하고 안정한 에멀젼입자가 형성되는 혼합유화제의 HLB 영역은 10.0∼10.8이었으며, 아울러 이 영역에서 오일상에 첨가되어 가용화되는 물의 양 또한 가장 많게 나타났다. O/W emulsions were prepared with the various phase inversion emulsification methods. The emulsifiers used were the polyoxyethylene(20)sorbitan monostearate and sorbitan sesquioleate, and the oil used was a liquid paraffin(dispersed phase). A portion of aqueous phase added during the phase inversion emulsification process is solubilized or emulsified into the oil phase to form a W/O primary emulsion. This primary emulsion eventually inverts to form an O/W final emulsion either by changing the dispersed phase volume(a catastrophic inversion) or by changing the surfactant`s affinity for the oil and water phases(a transitional inversion). A short-lived double emulsions of (W/O)/W type were formed in the process of the two-step phase inversion which adopted the direct emulsification method : O/W final emulsion was formed as follows, i.e., W/O→(W/O)/W double emulsion→O/W emulsion. Catastropic inversion occured in the two-step phase inversion emulsification method. In one-step phase inversion emulsification and three-step phase inversion emulsification, fine and homogeneous O/W emulsions were formed as follows, i.e., W/O→pseudomicroemulsion gel phase→O/W. In this method the transitional inversion occurred within constant concentration(5-17wt. %) of contineous phase. The more fine, homogeneous and viscous emulsion was prepared with the three-step phase inversion emulsification method than that of emulsions prepared with the two-step phase inversion emulsification method. The finest and most stable droplets were formed in 10.0∼10.8 HLB region of mixed surfactants and this HLB region had maximum amount of solubilizing water.