Cytogenetic assessment of <i>Lilium longiflorum × L. hansonii</i> revealed by genomic in situ hybridization (GISH)

Mazharul, I.M.,Reshma, Y.,Jung, J.M.,Mohammad, D.D.,Lim, K.B. International Society for Horticultural Science 2019 Acta Horticulturae Vol.1237 No.-

<P> Martagon (<I>Lilium hansonii;</I> MM) and <I>Longiflorum</I> (LL) are two major groups under the family <I>Liliaceae</I>, used for modern breeding to introduce new inter-genomic lily cultivars. Interspecific F<SUB>1</SUB> hybrids (LM) introduced through cut-style method between two diploid <I>Lilium longiflorum (2n=2x=24)</I> and <I>Lilium hansonii (2n=2x=24)</I> were evaluated cytogenetically by genomic in situ hybridization (GISH) technique. However, GISH analysis of F<SUB>1</SUB> interspecific (LM) hybrids showed equal chromosomal contribution from both female <I>Lilium longiflorum</I> (LL) and male <I>Lilium hansonii</I> (MM). Each of the parent contributed 12 chromosomes except three crosses i.e., two of <I>L. longiflorum</I> 'White Tower' × <I>L. hansonii;</I> (2x-1) and one of <I>L. longiflorum</I> 'Bright Tower' × <I>L. hansonii;</I> (2x-1). Among 11 inter-genomic crosses, 3 crosses failed (False hybrid) and 8 crosses (True hybrids) showed different ploidy level i.e., 2n=2x=24, 2n=2x-1=23 and 2n=2x-1=23 respectively. Recombinant chromosome usually not found in F<SUB>1</SUB> interspecific lily hybrids. Most often, genomic recombination occurred in the cross between two genetically different parents. Chromosome pairing and crossing over normally occurred during meiosis in backcross progenies. However, in this study, genome analysis (GISH) of F<SUB>1</SUB> hybrids (<I>L. longiflorum</I> 'White Tower' × <I>L. hansonii</I>) showed four recombinant sites including two M/L and two L/M recombinant chromosomes that denotes high genetic relationship between <I>L. longiflorum</I> and <I>L. hansonii.</I> </P>

Seedcoat removal and seed germination in Helianthus tuberosus L.

Lim, K.B.,Lee, H.J.,Lee, S.R.,Lee, J.I.,Ahn, S.D. 순천대학교 기초과학연구소 1990 基礎科學硏究誌 Vol.1 No.-

植物學的 種子 (botanical seed)에 의한 돼지감자의 實生繁殖을 시도하였다. J-42等 5種의 돼지감자 花器에서 種子를 채취하여 室內에 보관하여 오면서 發芽를 誘導하였으나 저장期間의 4個月 되는 時期까지 發芽시킬 수 없었다. 그러나 種子의 果皮(pericarp)와 種皮(seedcoat)를 모두 除去하는 處理에서 完全한 發芽誘導가 可能하였는데 特히 種子의 果皮除去만으로는 發芽誘導效果를 볼 수 없었다. 이러한 結果로부터 돼지 감자의 植物學的 種子의 경우 種子의 種皮가 種子의 不發芽에 깊이 관련되어 있음을 확인 할 수 있었다. 또한 돼지감자 種子의 種皮除去에 의한發芽誘導方法은 수집種에 따른 차이 없이 어느 種에서나 發芽誘導에 매우 效果的이었다. Pericarp and seedcoat removal treatments were tested to determine their effectiveness in the hard-to-germinate botanical seeds of Jerusalem artichoke. Fresh seed of five Helianthus tuberosus L. varieties were (A) untreated (B) water soaked overnight (C) soaked overnight followed by removal of pericarp or (D) soaked overnight followed by removal of pericarp and seedcoat. The results indicate that treatments which removed the pericarp and seedcoat were the very effective, giving germination of over 90%. A considerable increase in germination did not follow only the pericarp removal treatments. The factors inhibitory to germination of fresh Jerusalem artichoke seed is associated with the seedcoat. The removal treatment of pericarp and seedcost id recommended despite its complexity because it gives a high germination percentage and varies least from variety to variety. Helianthus tuberosus L., a biomass potential crop, is a member of the family compositae. The genus Helianthus has provided man with two food plants, the sunflower (H. annus)and the jersalem artichoke or topinambour (H. tuberosus). (3) The jersalem artichoke grown for its tubers, has always been an extremely minor crop, but it is still grown in many places as a food for man or livestock and propagation Jerusalem artichoke also flower and pruduce seed in head-like in florescences. Folwers are developed acropetally on flattened receptacles such that outermost flowers deffernt in genotype from others. But the variable response of varieties to the pericarp and seedcoat removal treatment lead us to this treatment as the best method for breaking hard-to-germinate trait in the botanical seed of H. tuberosus. Results obtained suggest that the factors inhibitory to germination is located in the seedcoat. These data also indicate inhibition may be physical rather than chemical since increased seed germination observed following pericarp removal could probably be attributer to damaged seedcoats, even thogh seedcoats appeared to be intact. However, it should be noted that this experiment was not designed to exclude chemical inhibitors as possible retardant to germination of freshly harvested seeds.

Structure of Li₅AlS₄ and comparison with other lithium-containing metal sulfides

Lim, H.,Kim, S.C.,Kim, J.,Kim, Y.I.,Kim, S.J. Academic Press 2018 Journal of Solid State Chemistry Vol.257 No.-

Lithium aluminum sulfide (Li<SUB>5</SUB>AlS<SUB>4</SUB>) was synthesized by solid state reaction, and its crystal structure was characterized by ab initio structure determination on the basis of powder neutron diffraction (ND) data. Li<SUB>5</SUB>AlS<SUB>4</SUB> was found to have monoclinic unit cell (space group, P2<SUB>1</SUB>/m) with the lattice parameters: a = 6.8583(4)A, b = 7.8369(4)A, c = 6.2488(4)A, and β = 90.333(4)<SUP>o</SUP>. This structure is built from a hexagonal close-packed (hcp) arrangement of sulfur atoms with a stacking sequence of ...ABAB.... The hcp sulfide lattice consists of two different double-sulfide layers alternately stacked along the c-axis. Between the first pair of sulfur layers all the tetrahedral interstices (T<SUP>+</SUP> and T<SUP>-</SUP> sites) are filled with lithium and aluminum atoms. All octahedral interstices between the second pair of sulfur layers are occupied by the remaining lithium atoms. The structure of Li<SUB>5</SUB>AlS<SUB>4</SUB> is compared with those of various lithium-containing metal sulfides like Li<SUB>2</SUB>FeS<SUB>2</SUB>, NaLiMS<SUB>2</SUB> (M = Zn, Cd), Li<SUB>4</SUB>GeS<SUB>4</SUB>, LiM'S<SUB>2</SUB> (M' = Al, Ga, In) and γ-Li<SUB>3</SUB>PS<SUB>4</SUB>. Each sulfide represents a specific distribution of lithium atoms in the lattice depending on how the octahedral and tetrahedral interstitial sites are filled. The low ionic conductivity of Li<SUB>5</SUB>AlS<SUB>4</SUB> (9.7 x 10<SUP>-9</SUP>Scm<SUP>-1</SUP> at 323K) relative to other sulfides may be due to the highly-ordered distribution of the lithium atoms in the layered structure and the lack of adjacent void spaces that can be used for lithium ion hopping.

Quasi-Monoenergetic Electron-Beam Generation Using a Laser Accelerator for Ultra-Short X-ray Sources

J Kim,고도경,석희용,H Jang,김형택,I Hwang,최일우,J Lim,J. Lee,J. H. Sung,K.-H. Hong,허민섭,N Hafz,유승훈,유태준,T. M. Jeong,V Kulagin,Y.-C. Noh 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.51 No.I

Two types of electron acceleration methods have been conducted to generate quasi-monoenergetic electron beams. Multi-MeV quasi-monoenergetic high-charge electron beams were generated at Korea Electrotechnology Research Institute (KERI) from self-modulated laser wakefield acceleration by using a collimator with a 2 TW (1.4 J/700 fs) Nd:glass/Ti:sapphire hybrid laser system and a supersonic nitrogen gas jet. The peak electron energy was 3.6 MeV, and the energy spread was 4 MeV. These electron beams are useful for the generation of short-pulse X-rays in the water window region, which is 250 eV -- 500 eV (2.5 -- 5 nm), by using Thomson scattering. The calcualted photon spectrum indicates the scattered photon covers the water window region. This can be used for a high spatial and temperal resolution microscope for medical imaing. To generate higher-energy electron beams with small energy spread, a laser wakefield acceleration experiment with a sharp downward electron density gradient was conducted with a 100 TW laser system at Advanced Photon Research Insistitute (APRI). With the electron density gradient, some background plasma electrons could be locally injected in the laser wake wave and a small energy spread was expected. Using the pre-pulse, we could generate sharp downward electron density gradients. The gradient scale length was 20 $\mu$m for a 25 \% density change. With this electron density gradient, we could get more reproducible electron beams than we could without the density gradient.

¹³¹I-labeled chitosan hydrogels for radioembolization: A preclinical study in small animals

Hwang, H.,Kim, K.I.,Kwon, J.,Kim, B.S.,Jeong, H.S.,Jang, S.J.,Oh, P.S.,Park, H.S.,Lim, S.T.,Sohn, M.H.,Jeong, H.J. Pergamon Press 2017 Nuclear medicine and biology Vol.52 No.-

<P>Advances in knowledge: Transarterial embolization is a conceivable treatment option for patients with inoperable liver cancer to mitigate the disease progression. Recently, we have developed chitosan-based hydrogel microparticles. In the present study, the hydrogel microparticles were radiolabeled with I-131 for treatment of liver cancer. Our results demonstrated that a hepatic arterial injection of I-125 -labeled Chi resulted in substantial liver accumulation, which was accompanied by virtually no extrahepatic deposition. The results of the present study also showed that administration of I-131 Chi markedly suppressed tumor growth, compared to controls and to animals receiving unlabeled Chi. I-131 -labeled chitosan hydrogel microparticles represent a new therapeutic approach for treatment of liver cancer. (C) 2017 Elsevier Inc. All rights reserved.</P>

건국 우유와 유제품의 소비성향에 관한 조사연구

임종우,김종우,조광호,김영주,유제현,윤여창,장주익,권일경,안효일,이용규,이부응,박종래,이헌종 경상대학교 축산진흥연구소 1989 畜産振興硏究所報 Vol.16 No.1

I발표장 : 촉매 및 반응공학2 ; I-54 : 일산화탄소의 선택산화반응용 CuO-CeO2 촉매의 활성에 미치는 귀금속 첨가 효과

정창렬 ( C. R. Jung ),한종희 ( J. Han ),남석우 ( S. W. Nam ),임태훈 ( T. -h. Lim ),홍성안 ( S. -a. Hong ),이호인 ( H. -i. Lee ) 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.1

Far Ultraviolet Observations of the Loop I/North Polar Spure Region

J. W. Park,K. W. Min,J. H. Shin,I. J. Kim,Y. M. Lim,K. I. Seon,D. H. Lee 한국천문학회 2007 天文學會報 Vol.32 No.1

Therapeutic targeting of subdural medulloblastomas using human neural stem cells expressing carboxylesterase

Lim, S-H,Choi, S A,Lee, J Y,Wang, K-C,Phi, J H,Lee, D-H,Song, S H,Song, J H,Jin, X,Kim, H,Lee, H J,Lim, I,Kim, S U,Kim, S-K Nature America, Inc. 2011 Cancer gene therapy Vol.18 No.11

The prognosis of medulloblastoma has improved significantly because of advances in multi-modal treatments; however, metastasis remains one of the prognostic factors for a poor outcome and is usually associated with tumor recurrence. We evaluated the migratory potential and therapeutic efficacy of genetically engineered human neural stem cells (NSCs) that encode a prodrug enzyme in the subdural medulloblastoma model. We genetically modified HB1.F3 (F3) immortalized human NSCs to express rabbit carboxylesterase (rCE) enzyme, which efficiently converts the prodrug CPT-11 (Irinotecan) into an active anti-cancer agent (SN-38). To simulate clinical metastatic medulloblastomas, we implanted human medulloblastoma cells into the subdural spaces of nude mice. rCE expressing NSCs (F3.rCE) were labeled with fluorescence magnetic nanoparticle for in vivo imaging. The therapeutic potential of F3.rCE was confirmed using a mouse subdural medulloblastoma model. The majority of intravenously (i.v.) injected, F3.rCE cells migrated to the subdural medulloblastoma site and a small number of F3.rCE cells were found in the lungs, pancreas, kidney and liver. Animals that received F3.rCE cells in combination with prodrug CPT-11 survived significantly longer (median survival: 142 days) than control mice that received F3.rCE cells only (median survival: 80 days, P<0.001) or CPT-11 only (median survival: 118 days, P<0.001). In conclusion, i.v. injected F3.rCE NSCs were able to target subdural medulloblastomas and demonstrate therapeutic efficacy. Our study provides data that supports further investigation of stem-cell-based gene therapy against metastatic medulloblastomas.

FUV Emission and Absorption Feature of the Loop I/North Polar Spur Region

J. W. Park,K. W. Min,J. H. Shin,I. J. Kim,Y. M. Lim,K. I. Seon,D. H. Lee 한국천문학회 2007 天文學會報 Vol.32 No.2