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Beamline Automation of RIKEN Structural Genomics Beamlines
Ida, Koh,Yamamoto, Masaki,Kumasaka, Takashi,Ueno, Go,Kanda, Hiroyuki,Miyano, Masashi,Ishikawa, Tetsuya Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2
RIKEN Structural Genomics Beamlines have been constructed for the crystallographic analysis in the structural genomics research at synchrotron radiation facility SPring-8. Synchrotron radiation accelerates the crystallographic analysis of protein structure. The target of the research and development is focused on the automatic beamline operation to maximize beamline efficiency. We are developing the sample management system, which is composed of the sample auto-changer and the database system, for high-throughput data collection. The sample management system and the beamline operating system make it possible to execute automatic data collection without any operators. The beamlines will be ready for user operation in autumn 2002. The concept of automatic beamline operation and the present status of RIKEN Structural Genomics Beamlines will be presented.
Nozue Goro,Fujiwara Hidenori,Hamamoto Satoru,Kiss Takayuki,Tsutsumi Miwa,Oura Masaki,Ishikawa Tetsuya,Motouri Azusa,Suzuki Shintaro,Tamura Ryuji,Sekiyama Akira 한국물리학회 2023 새물리 Vol.73 No.12
We have performed Ce M4,5-edge X-ray absorption (XAS) and Ce 3d-4f resonance photoemission (RPES) spectroscopies on quasicrystal approximant Au59.2Ga25.7Ce15.1. The 3d94f1 final state originating from the itinerant 4f electronic states is negligible in the Ce M4,5-edge XAS. The Ce 3d-4f RPES spectrum shows the relatively large the spectral weight of the 4f0 final state to that of the 4f1 final state, reflecting the weak hybridization between the localized 4f orbitals and the conduction bands. The localized 4f electronic states in Au59.2Ga25.7Ce15.1 are revealed by both spectroscopies.
Dendritic planarity of Purkinje cells is independent of Reelin signaling
Kim, Jinkyung,Park, Tae-Ju,Kwon, Namseop,Lee, Dongmyeong,Kim, Seunghwan,Kohmura, Yoshiki,Ishikawa, Tetsuya,Kim, Kyong-Tai,Curran, Tom,Je, Jung Ho Springer Berlin Heidelberg 2015 BRAIN STRUCTURE AND FUNCTION Vol.220 No.4
<P>The dendritic planarity of Purkinje cells is critical for cerebellar circuit formation. In the absence of Crk and CrkL, the Reelin pathway does not function resulting in partial Purkinje cell migration and defective dendritogenesis. However, the relationships among Purkinje cell migration, dendritic development and Reelin signaling have not been clearly delineated. Here, we use synchrotron X-ray microscopy to obtain 3-D images of Golgi-stained Purkinje cell dendrites. Purkinje cells that failed to migrate completely exhibited conical dendrites with abnormal 3-D arborization and reduced dendritic complexity. Furthermore, their spines were fewer in number with a distorted morphology. In contrast, Purkinje cells that migrated successfully displayed planar dendritic and spine morphologies similar to normal cells, despite reduced dendritic complexity. These results indicate that, during cerebellar formation, Purkinje cells migrate into an environment that supports development of dendritic planarity and spine formation. While Reelin signaling is important for the migration process, it does not make a direct major contribution to dendrite formation.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1007/s00429-014-0780-2) contains supplementary material, which is available to authorized users.</P>
Fixed target single-shot imaging of nanostructures using thin solid membranes at SACLA
Nam, Daewoong,Kim, Chan,Kim, Yoonhee,Ebisu, Tomio,Gallagher-Jones, Marcus,Park, Jaehyun,Kim, Sunam,Kim, Sangsoo,Tono, Kensuke,Noh, Do Young,Yabashi, Makina,Ishikawa, Tetsuya,Song, Changyong IOP 2016 Journal of Physics B: Atomic, Molecular and Optica Vol.49 No.3
<P>Single-shot imaging using femtosecond x-ray pulses from x-ray free electron lasers (XFELs) has revealed high-resolution structures of Au nanoparticles and biological macromolecular complexes. The x-ray pulse power of 10 GW is enough to vaporize specimen when it is focused on a few microns area. This makes it essential to have a single particle loader to provide fresh samples to the x-ray pulses. In this paper we introduce single-shot imaging at Spring-8 Angstrom Compact Free Electron Laser (SACLA) to investigate various types of specimen, from metallic nanoparticles to biological macromolecules, prepared on Si<SUB>3</SUB>N<SUB>4</SUB> membrane. A significant reduction in sample consumption is achieved while maintaining a data acquisition rate of 30 Hz, which is compatible with the current SACLA operation rate.</P>
Sun, Zhibin,Fan, Jiadong,Li, Haoyuan,Liu, Huajie,Nam, Daewoong,Kim, Chan,Kim, Yoonhee,Han, Yubo,Zhang, Jianhua,Yao, Shengkun,Park, Jaehyun,Kim, Sunam,Tono, Kensuke,Yabashi, Makina,Ishikawa, Tetsuya,So American Chemical Society 2018 ACS NANO Vol.12 No.8
<P>It has been proposed that the radiation damage to biological particles and soft condensed matter can be overcome by ultrafast and ultraintense X-ray free-electron lasers (FELs) with short pulse durations. The successful demonstration of the “diffraction-before-destruction” concept has made single-shot diffraction imaging a promising tool to achieve high resolutions under the native states of samples. However, the resolution is still limited because of the low signal-to-noise ratio, especially for biological specimens such as cells, viruses, and macromolecular particles. Here, we present a demonstration single-shot diffraction imaging experiment of DNA-based structures at SPring-8 Angstrom Compact Free Electron Laser (SACLA), Japan. Through quantitative analysis of the reconstructed images, the scattering abilities of gold and DNA were demonstrated. Suggestions for extracting valid DNA signals from noisy diffraction patterns were also explained and outlined. To sketch out the necessary experimental conditions for the 3D imaging of DNA origami or DNA macromolecular particles, we carried out numerical simulations with practical detector noise and experimental geometry using the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, USA. The simulated results demonstrate that it is possible to capture images of DNA-based structures at high resolutions with the technique development of current and next-generation X-ray FEL facilities.</P> [FIG OMISSION]</BR>