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Yoon, Hye-Ryeon,Paik, Young-Sook The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.5
Davallialactone (1) and inoscavin A (2) isolated from Phellinus linteus showed significant prolyl endopeptidase competitive inhibition with $IC_{50}$ values of 20.0${\pm}$0.1 and 12.2${\pm}$0.1 ${\mu}M$ and $K_i$ values of 15.6${\pm}$0.3 and 8.3${\pm}$0.1 ${\mu}M$, respectively. They also exhibited strong antioxidant capacities against the ABTS radical system with $EC_{50}$ values of 2.61${\pm}$0.01, 13.3${\pm}$0.05 ${\mu}M$, respectively.
Hye Ryeon Yoon,Young Sook Paik 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.5
Davallialactone (1) and inoscavin A (2) isolated from Phellinus linteus showed significant prolyl endopeptidase competitive inhibition with IC50 values of 20.0±0.1 and 12.2±0.1 ?M and Ki values of 15.6±0.3 and 8.3±0.1 ?M, respectively. They also exhibited
Prolyl Endopeptidase Inhibitory Activity of Two Styrylpyranones from Phellinus linteus
( Hye Ryeon Yoon ),( Ah Reum Han ),( Young Sook Paik ) 한국응용생명화학회(구 한국농화학회) 2013 Journal of Applied Biological Chemistry (J. Appl. Vol.56 No.3
Two styrylpyranones (1 and 2) were isolated from the CH2Cl2-soluble fraction of Phellinus linteus and their structures were established by extensive Nuclear magnetic resonance (NMR) spectral data as inoscavin E and C. Both compounds showed significant prolyl endopeptidase inhibitory activity with IC50 values of 4.26±0.14 and 4.08±0.04 μM and Ki values of 1.50±0.02 and 1.43±0.03 μM, respectively. They also exhibited antioxidant capacities against the ABTS radical system with EC50 values of 6.47±0.05 and 7.64±0.06 μM, respectively.
Yoon, Hye Ryeon,Lee, Jeong Min,Jung, Juyeon,Lee, Chang-Soo,Chung, Bong Hyun,Jung, Yongwon The Royal Society of Chemistry 2014 The Analyst Vol.139 No.1
<P>Poor specificity has been a lingering problem in many microRNA profiling methods, particularly surface hybridization-based methods such as microarrays. Here, we carefully investigated surface hybridization and dissociation processes of a number of sequentially similar microRNAs against nucleic acid capture probes. Single-base mismatched microRNAs were similarly hybridized to a complementary DNA capture probe and thereby poorly discriminated during conventional stringent hybridization. Interestingly, however, mismatched microRNAs showed significantly faster dissociation from the probe than the perfectly matched microRNA. Systematic analysis of various washing conditions clearly demonstrated that extremely high specificity can be obtained by releasing non-specific microRNAs from assay surfaces during a stringent and controlled dissociation step. For instance, compared with stringent hybridization, surface dissociation control provided up to 6-fold better specificity for Let-7a detection than for other Let-7 family microRNAs. In addition, a synthetically introduced single-base mismatch on miR206 was almost completely discriminated by optimized surface dissociation of captured microRNAs, while this mismatch was barely distinguished from target miR206 during stringent hybridization. Furthermore, a single dissociation condition was successfully used to simultaneously measure four different microRNAs with extremely high specificity using melting temperature-equalized capture probes. The present study on selective dissociation of surface bound microRNAs can be easily applied to various hybridization based detection methods for improved specificity.</P> <P>Graphic Abstract</P><P>A detailed analysis of microRNA surface hybridization and dissociation offered a generally applicable hybridization strategy for microRNA detection with highly improved specificity. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3an01688a'> </P>