Macrophage activation by glycoprotein isolated from Dioscorea batatas

Huong, Pham Thi Thu,Jeon, Young-Jin Korean Society of ToxicologyKorea Environmental Mu 2011 Toxicological Research Vol.28 No.3

We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) activates macrophage function. Analysis of the infiltration of macrophages into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages into the peritoneal cavity. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including IL-$1{\beta}$, TNF-${\alpha}$, and IL-6 in mouse peritoneal macrophages. GDB increased the expression of IL-$1{\beta}$, TNF-${\alpha}$, and IL-6. Cytokine induction by GDB was further confirmed by RT-PCR and ELISA in mouse macrophage cell line, RAW264.7 cells. Treatment of RAW264.7 cells with GDB produced strong induction of NF-${\kappa}B$ DNA binding and MAPK phosphorylation, markers for macrophage activation and important factors for cytokine gene expression. Collectively, this series of experiments indicates that GDB stimulates macrophage activation.

Pretreatment of Sweet Sorghum Bagasse (SSB) using Green Liquor (GL) for Biorenewable Sugar Production

Thi Thu Huong Pham,Nhuan P. Nghiem,Tae Hyun Kim 한국신재생에너지학회 2017 한국신재생에너지학회 학술대회논문집 Vol.2017 No.9

Characterization and Immunopotentiating Effects of the Glycoprotein Isolated from Dioscorea Batatas

Huong, Pham Thi Thu,Lee, Chan-Ho,Li, Mei-Hong,Lee, Min-Young,Kim, Joon-Ki,Lee, Sun-Mee,Seon, Jong-Heum,Lee, Dong-Cheol,Jeon, Young-Jin The Korean Society of Pharmacology 2011 The Korean Journal of Physiology & Pharmacology Vol.15 No.2

We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) has immunostimulatory effects including macrophage activation. Analysis of infiltration of inflammatory cells into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages, lymphocytes, neutrophils, and monocytes into the peritoneal cavity. Treatment of spleen cells isolated from C57BL/6 mice with GDB significantly increased the proliferation of B cells and T cells induced by LPS and ConA, respectively. Treatment with GDB significantly increased the cytolytic capacity of NK cells and macrophages against YAC-1 and B16 cells, respectively. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including iNOS, IL-1${\beta}$, and TNF-${\alpha}$ in mouse macrophage cell line, RAW 264.7 cells. RT-PCR and ELISA showed that GDB increased the expression of IL-1${\beta}$, and TNF-${\alpha}$, whereas iNOS was not induced by GDB. Collectively, this series of experiments indicates that GDB stimulates immune system including macrophage activation.

Characterization and Immunopotentiating Effects of the Glycoprotein Isolated from Dioscorea Batatas

Pham Thi Thu Huong,Chan-Ho Lee,Mei Hong Li,Min Young Lee,김준기,이선미,Jong-Heum Seon,이동철,전영진 대한약리학회 2011 The Korean Journal of Physiology & Pharmacology Vol.15 No.2

We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) has immunostimulatory effects including macrophage activation. Analysis of infiltration of inflammatory cells into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages, lymphocytes, neutrophils, and monocytes into the peritoneal cavity. Treatment of spleen cells isolated from C57BL/6 mice with GDB significantly increased the proliferation of B cells and T cells induced by LPS and ConA, respectively. Treatment with GDB significantly increased the cytolytic capacity of NK cells and macrophages against YAC-1 and B16 cells, respectively. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including iNOS, IL-1β, and TNF-α in mouse macrophage cell line, RAW 264.7 cells. RT-PCR and ELISA showed that GDB increased the expression of IL-1β, and TNF-α, whereas iNOS was not induced by GDB. Collectively, this series of experiments indicates that GDB stimulates immune system including macrophage activation.

Distribution of Competitiveness and Foreign Direct Investment using Autoregressive Distributed Lag Model

PHAM Huong Thi Thu,PHAM Nga Thi 한국유통과학회 2022 유통과학연구 Vol.20 No.8

Purpose: Research on attracting foreign direct investment (FDI) plays an important role in helping provinces attract more FDI projects. However, with local competition, FDI enterprises also have to consider their investment. This study evaluates the provincial competitiveness to attract FDI in Thai Nguyen province, a province of Vietnam. In which provincial distribution of competitiveness is measured through nine indicators. Research design, data, and methodology: The study collects data (FDI and the provincial competitiveness index) from 2006 to 2020. The study uses Autoregressive Distributed Lag (ARDL) to text the impact of distribution of competitivenes on foreign direct investment. With time-series, the ARDL is suitable for data analysis. Results: The regression results indicate that the competition index of market entry and informal costs negatively impact attracting FDI into the province; The human resource training quality index has a positive effect on FDI. The results show that FDI enterprises pay much attention to business establishment procedures, hidden costs, and quality of human resources in the province. Conclusions: At the same time, in terms of practice, the results of this study, the authors also offer solutions to help improve the ability to attract FDI into Thai Nguyen province. The significant provincial competitiveness indicators should be taken into account for improvement first.

Macrophage activation by glycoprotein isolated from Dioscorea batatas

Pham Thi Thu Huong,Young Jin Jeon 한국독성학회 2011 Toxicological Research Vol.27 No.3

We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) activates macrophage function. Analysis of the infiltration of macrophages into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages into the peritoneal cavity. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including IL-1β, TNF-α, and IL-6 in mouse peritoneal macrophages. GDB increased the expression of IL-1β, TNF-α, and IL-6. Cytokine induction by GDB was further confirmed by RT-PCR and ELISA in mouse macrophage cell line, RAW264.7 cells. Treatment of RAW264.7 cells with GDB produced strong induction of NF-κB DNA binding and MAPK phosphorylation, markers for macrophage activation and important factors for cytokine gene expression. Collectively, this series of experiments indicates that GDB stimulates macrophage activation.

Recovery of Acetic Acid from An Ethanol Fermentation Broth by Liquid-Liquid Extraction (LLE) Using Various Solvents

( Thi Thu Huong Pham ),( Tae Hyun Kim ),( Byung Hwan Um ) 한국화학공학회 2015 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.53 No.6

Liquid-liquid extraction (LLE) using various solvents was studied for recovery of acetic acid from a synthetic ethanol fermentation broth. The microbial fermentation of sugars presented in hydrolyzate gives rise to acetic acid as a byproduct. In order to obtain pure ethanol for use as a biofuel, fermentation broth should be subjected to acetic acid removal step and the recovered acetic acid can be put to industrial use. Herein, batch LLE experiments were carried out at 25°C using a synthetic fermentation broth comprising 20.0 g l-1 acetic acid and 5.0 g l-1 ethanol. Ethyl acetate (EtOAc), tri-n-octylphosphine oxide (TOPO), tri-n-octylamine (TOA), and tri-n-alkylphosphine oxide (TAPO) were utilized as solvents, and the extraction potential of each solvent was evaluated by varying the organic phase-to-aqueous phase ratios as 0.2, 0.5, 1.0, 2.0, and 4.0. The highest acetic acid extraction yield was achieved with TAPO; however, the lowest ethanol-to-acetic acid extraction ratio was obtained using TOPO. In a single-stage batch extraction, 97.0 % and 92.4 % of acetic acid could be extracted using TAPO and TOPO when the ratio of organic-to-aqueous phases is 4:1 respectively. A higher solvent-to-feed ratio resulted in an increase in the ethanol-to-acetic acid ratio, which decreased both acetic acid purity and acetic acid extraction yield.

Synergistic Induction of iNOS by IFN-g and Glycoprotein Isolated from Dioscorea batatas

Pham Thi Thu Huong,Min Young Lee,Kun Yeong Lee,In Youp Chang,Seog Ki Lee,Sang Pil Yoon,Dong-Cheol Lee,Young Jin Jeon 대한생리학회-대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.6

Dioscorea species continue to be used in traditional Chinese medicine, and represent a major source of steroid precursors for conventional medicine. In the previous study, We isolated glycoprotein (GDB) from Dioscorea batatas, characterized, and demonstrated immunostimulating activity in C57BL/6 mice. The aim of this study was to investigate the mechanism whereby GDB activates macrophages. Macrophages activation by GDB was investigated by analyzing the effects of GDB on nitric oxide (NO) production, iNOS expression, mitogen activated protein kinase (MAPK) phosphorylation, and transcription factor activation. In the presence of IFN-Ճ, GDB strongly stimulated macrophages to express iNOS and produce NO. Furthermore, the activation of p38 was synergistically induced by GDB plus IFN-Ճ, but SB203580 (a p38 inhibitor) inhibited GDB plus IFN-Ճ-induced p38 activation. This study indicates that GDB is an important activator of macrophages. Furthermore, due to the critical role that macrophage activation plays in innate immune response, the activation effects of GDB on macrophages suggest that GDB may be a useful immunopotentiating agent.

Place Brand Equity and Domestic Investors' Choice: A Case Study in Vietnam

PHAM, Huong Thi Thu,PHAM, Nga Thi Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.10

Selecting an investment location is one of the most crucial decisions of investors as it has a great influence on the operation and development of the business in the future. At the same time, the attraction of localities will bring advantages for socio-economic development for the localities invested. Investors are interested in localities that have the potential to invest. The study focused on analyzing and testing the influence of place brand equity on the choice of investment locations of domestic investors through a regression analysis using 425 survey samples of investors in Phu Tho province, one of the northern industrial zones in Vietnam. Research results showed that 56.5% of investment decisions depended on factors from place brands. In addition, in the decision-making process for choosing investment locations, brand awareness factor had the greatest impact on investor's decisions, followed by brand image and brand personality, and finally brand confidence had the smallest impact. Therefore, in the coming time, in order to retain and attract domestic investors to choose Phu Tho as an investment and business destination, it is necessary to increase the value of place brand equity and implement solutions to promote place brands to investors.

Foreign Direct Investment -Small and Medium Enterprises Linkages and Global Value Chain Participation: Evidence from Vietnam

NGUYEN, Thi Minh Thu,NGUYEN, Thi Tuong Anh,NGUYEN, Thi Thuy Vinh,PHAM, Huong Giang Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.3

Using a multinomial logit model with the panel-data set of Vietnam manufacturing firms, this paper investigates the impacts of foreign direct investment (FDI) - small and medium enterprises (SMEs) linkages and other factors on SMEs' participation in the global value chain (GVC). We consider GVC firms are those engaging in any of the three modes including (i) using domestic inputs to export (D2E), (ii) using imported inputs to produce for the domestic market (I2P), (iii) using imported inputs to export (I2E). We discover that FDI-SME linkages statistically encourage Vietnamese SMEs to integrate into the GVC via I2P and I2E, while no statistical association between FDI-SME linkage and D2E participation is found. GVCs participation likelihood is also positively correlated with the introduction of new product introduction. The establishment of firms' production facilities in industrial zones and foreign ownership are both reported to be significantly decisive factors to SMEs' decisions on GVC participation. Besides, there is a strong association between firms' attributes, i.e. employment, capital intensity as well as financial access, and their participation in the GVC. Local governance quality (proxied by the Provincial Competitiveness Index) and the share of skilled labor at the province-level can facilitate firms' integration into GVCs, while greater market concentration may be a hurdle to such potential.