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Rapid Identification and Characterization of Antioxidants from Ligularia fischeri
Xiang-Lan Piao,Xiao-Yuan Mi,Yan-Ze Tian,Qian Wu,Hui-Shan Piao,Zhikai Zeng,Xiangshu Piao,Ding Wang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.12
The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH2Cl2, BuOH, and H2O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2''-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC50 values of 1.31 and 7.09 μg/mL, respectively. They have not been reported from L. fischeri yet.
Aloe Emodin-Induced Apoptosis in t-HSC/Cl-6 Cells Involves a Mitochondria-Mediated Pathway
Lian, Li-Hua,Park, Eun-Jeon,Piao, Hui-Shan,Zhao, Yu-Zhe,Sohn, Dong Hwan Blackwell Science, Ltd 2005 Basic & Clinical Pharmacology & Toxicology Vol.96 No.6
<P>Abstract: </P><P>The aim of our study was to clarify the apoptosis pathway induced by aloe emodin, an hydroxyanthraquinone present in <I>aloe vera</I> leaves, in rat hepatic stellate cells transformed by simian virus 40 (t-HSC/Cl-6), which retain the features of activated rat stellate cells. Apoptosis was determined by DNA fragmentation, caspase activity assay and western blotting analysis. Treatment of t-HSC/Cl-6 cells with 12.5, 25, or 50 &mgr;M aloe emodin inhibited t-HSC/Cl-6 cell viability in a dose- and time-dependent manner. The induction of apoptosis by aloe emodin was confirmed by typical DNA ladder formation and annexin v-propidium iodide flow-cytometric analysis. Aloe emodin treatment of t-HSC/Cl-6 cells caused activation of caspase-3 and caspase-9, detected with a caspase activity assay, although no change was observed in caspase-8 activity. Western blotting showed caspase-3 and caspase-9 active forms and the subsequent proteolytic cleavage of poly(ADP-ribose) polymerase. Aloe emodin induced mitochondrial membrane depolarization. Our data also show that cytochrome c increased in the cytosol but decreased in the mitochondria in a time-dependent manner. Increased Bax and unchanged Bcl-2 levels resulted in an increased Bax/Bcl-2 ratio. Thus, our research provides evidence that aloe emodin-induced apoptosis involves a mitochondria-associated apoptosis pathway.</P>
A Study of Standardization of Platycodon grandiflorum A.DC., Campanulaceae with Platycodin D
Kim, Ho-chul,Ahn, Duk-kyun,Kang, Chul-hun,Piao, Hui-shan INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 1998 JOURNAL OF ORIENTAL MEDICINE Vol.3 No.1
In this study, a new purification method of platycodin D and a standardization method for evaluation of Platycodon grandiflorum A. DC., are introduced. Platycodin D was isolated from Platycodon grandiflorum A. DC., Campanulaceae, using high performance liquid chromatography (HPLC) with a gradient (water and acetonitrile) program. The purified platycodin D was characterized with Fast Atom Bombardment mass spectrometer, thin layer chromatography and ultra-viloet(UV) spectrophotometer. These data demonstrate that its molecular weight of 1224, which is consistent with that of the platycodin D and its UV-spectrum, is similar to that of a compound with a double bond. The Rf value from thin layer chromatography (TLC) is that expected for the purified platycodin D. The purified compound was used as a standard in evaluating the quality of Platycodon grandiflorum A. DC., Campanulaceae from various sources. The result indicates that the wild Platycodon grandiflorum A. DC., Campanulaceae contains 1.78 times more platycodin D than the cultured one.