Feeding and related morphology of salivary glands of Bradysia odoriphaga (Diptera: Sciaridae)

Huan-Huan Gao,Yi-Fan Zhai,Xue Cao,Xian-Hong Zhou,Zhong-Yan Wang,Fang Wang,Hao Chen,Yi Yu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2

The amount of Bradysia odoriphaga larval feeding at each developmental stage and the related morphology of salivary glands were investigated in this study. The amount of daily feeding in the third instar was greatest among all the developmental stageswhile the amount of cumulative feeding in the fourth-instar accounted for 53.56% of the total amount of larval feeding. The daily amount of feeding by B. odoriphaga during the whole larval stage peaked in the 8th and 14th day after hatching. The paired and transparent salivary glands of B. odoriphaga were characterized by two distinct regions: an anterior region and a posterior region. At the fourth-instar and pupal stage, the length of salivary glands decreased significantly compared with that of the third-instar, and the integrity of epithelium cytoplasm in the salivary glands also decreased markedly because of the formation of cytoplasmic vacuoles and degradation of rough endoplasmic reticulum and mitochondria. We concluded that the decrease of daily feeding amount in the last instar was related to the structural change of salivary gland closely. The function of salivary glands in B. odoriphaga was mainly secretion of silk and cocooning-like materials during the last larval instar.

Impact of selected fungi from an artificial diet on the growth and development of Drosophila suzukii (Diptera: Drosophilidae)

Huan-Huan Gao,Nan Xu,Hao Chen,Qian Liu,Qian-Yun Pu,Dong-Yun Qin,Yi-Fan Zhai,Yi Yu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

Drosophila suzukii (Diptera: Drosophilidae) is one of the very few Drosophila species which are able to lay eggs and feed on healthy ripening fruit. Adults and larvae are all able to obtain the nutrition in the decaying food, which will generate many microorganisms. However, the relationship between D. suzukii and microorganism is poorly understood. In this study, 13 species of fungi were identified in an artificial diet fed by D. suzukii. Short-term and continuously life table experiments were conducted to determine the impact of three of these fungus species including Geotrichum candidum, Talaromyces minioluteus and Actinomucor elegans on the growth and development of Drosophila suzukii. Results revealed that, compared to the control, G. candidum, T.minioluteus and A. elegans increased the mortality of D. suzukii adults in the short time, while extended the developmental time of pupal D. suzukii by 18.00%, 16.22% and 26.44%, respectively in the life table experiment. T. minioluteus reduced the total longevity of D. suzukii by 15.52%, while A. elegans enhanced the total longevity by 25.96%. G. candidum and A. elegans increased the fecundity bymore than two folds. T. minioluteus elongated the mean generation time (T) by 31.34%, whereas G. candidum shortened it by 15.26% but increased the net reproductive rate (R0) by 217.76%, intrinsic rate of increase (r) by 88.89% and finite rate of increase (λ) by 9.17%. It was concluded that G. candidumand A. elegans significantly had the beneficial effect on the growth and reproduction of D. suzukii, however, T. minioluteus had the negative effect on D. suzukii. Our results could provide a new integrated pest management strategy for D. suzukii which would be discussed in this study.

Distributed Fuzzy Resilient Tracking for Nonlinear MASs Under DoS Attacks

Shu-Huan Gao,Mengmeng Chen,Chao Deng 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.10

In this paper, the distributed fuzzy resilient tracking problem for a class of second-order nonlinear multiagent systems (MASs) under denial-of-service (DoS) attacks is addressed. Compared with the existing distributed fuzzy tracking results, a more general class of second-order MASs with DoS attacks under combined output observable situation are considered in this paper. Under DoS attacks, the second-order derivative of the traditional distributed observer does not exist, while this signal is necessary to solve the distributed fuzzy tracking problem for second-order MASs when using the backstepping method. To solve the problem, a hierarchical control method that consists of a cooperative resilient observer and a distributed controller is proposed. Specifically, a new distributed resilient observer state with a second-order derivative and also the function of defending network attacks is designed based on a combined output observable condition. By using the backstepping technique, decentralized fuzzy adaptive resilient controllers are designed to achieve distributed tracking under DoS attacks. It is shown that the distributed fuzzy resilient tracking problem can be solved under our designed method. Finally, second-order nonlinear MASs are provided to verify the effectiveness of our method.

Seismic behavior of SFRC shear wall with CFST columns

Danying Gao,Pei-Bo You,Li-Juan Zhang,Huan-Huan Yan 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.5

The use of reinforced concrete (RC) shear wall with concrete filled steel tube (CFST) columns and steel fiber reinforced concrete (SFRC) shear wall has aroused widespread attention in recent years. A new shear wall, named SFRC shear wall with CFST columns, is proposed in this paper, which makes use of CFST column and SFRC shear wall. Six SFRC shear wall with CFST columns specimens were tested under cyclic loading. The effects of test parameters including steel fiber volume fraction and concrete strength on the failure mode, strength, ductility, rigidity and dissipated energy of shear wall specimens were investigated. The results showed that all tested shear wall specimens exhibited a distinct shear failure mode. Steel fibers could effectively control the crack width and improve the distribution of cracks. The load carrying and energy dissipation capacities of specimens increased with the increase of steel fiber volume fraction and concrete strength, whilst the ductility of specimens increased with the increase of steel fiber volume fraction and the decrease of concrete strength.

Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)

Feng-Shan Gao,Xian-zhu Xia,Yu-Wei Gao,Ya-Duo Bai,Xiao-Huan Zou,Gui-Xue Hu 대한수의학회 2009 Journal of Veterinary Science Vol.10 No.3

Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

Expression and Clinical Significance of Osteopontin in Calcified Breast Tissue

Huan, Jin-Liang,Xing, Li,Qin, Xian-Ju,Gao, Zhi-Guang,Pan, Xiao-Feng,Zhao, Zhi-Dong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Osteopontin (OPN) is an integrin-binding protein, believed to be involved in a variety of physiological cellular functions. The physiology of OPN is best documented in the bone where this secreted adhesive glycoprotein appears to be involved in osteoblast differentiation and bone formation. In our study, we used semi-quantitative RT-PCR of osteopontin in calcification tissue of breast to detect breast cancer metastasis. The obtained data indicate that the expression of osteopontin is related to calcification tissue of breast, and possibly with the incidence of breast cancer. The expression strength of OPN by RT-PCR detection was related to the degree of malignancy of breast lesions, suggesting a close relationship between OPN and breast calcification tissue. The results revealed that expression of OPN mRNA is related to calcification of breast cancer tissue and to the development of breast cancer. Determination of OPN mRNA expression can be expected to be a guide to clinical therapy and prediction of the prognosis of breast cancer patients.

Processing, microstructure and mechanical properties of porous silicon nitride by a slip casting method using petroleum coke as pore former

Huan-Rui Wang,Ji-Qiang Gao,Fang-Li Yu,Yuan Li,Jian-Feng Yang 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.6

In this study, porous Si3N4 ceramics were obtained by a slip casting method which would be easier applied in industry. The most stable and dispersing aqueous suspension was obtained from a solid slurry content of 56.2%, a carboxymethyl cellulose concentration of 0.3% as the adhesive and 0.05% glycerol as the defoamer. Petroleum coke was used as pore-former, and at the same time, it was helpful for the stability of the suspension. The burning-out of the petroleum coke seemed to be an economical way for increasing the porosity of porous Si3N4. After sintering at 1,800℃ for 2 h, samples with a pore size about 10 μm, porosities of 45-60%, and flexural strength of 52.0-112.1MPa were obtained. In this study, porous Si3N4 ceramics were obtained by a slip casting method which would be easier applied in industry. The most stable and dispersing aqueous suspension was obtained from a solid slurry content of 56.2%, a carboxymethyl cellulose concentration of 0.3% as the adhesive and 0.05% glycerol as the defoamer. Petroleum coke was used as pore-former, and at the same time, it was helpful for the stability of the suspension. The burning-out of the petroleum coke seemed to be an economical way for increasing the porosity of porous Si3N4. After sintering at 1,800℃ for 2 h, samples with a pore size about 10 μm, porosities of 45-60%, and flexural strength of 52.0-112.1MPa were obtained.

Effects of feed intake restriction during late pregnancy on the function, anti-oxidation capability and acute phase protein synthesis of ovine liver

Huan Yang,Ying Wang,Chi Ma,Chuan Sun,Yingchun Liu,Kaifeng Wu,Ming Li,Gerelt Borjigin,Feng Gao 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.2

Objective: An experiment was conducted to investigate the effects of feed intake restriction during late pregnancy on the function, anti-oxidation capability and acute phase protein synthesis of ovine liver. Methods: Eighteen time-mated ewes with singleton fetuses were allocated to three groups: restricted group 1 (RG1, 0.18 MJ ME/kg W0.75 d, n = 6), restricted group 2 (RG2, 0.33 MJ ME/kg W0.75 d), n = 6) and a control group (CG, ad libitum, 0.67 MJ ME/kg W0.75 d, n = 6). The feed restriction period was from 90 days to 140 days of pregnancy. Results: The ewe’s body weight, liver weights, water, and protein content of liver in the restricted groups were reduced compared with the CG group (p<0.05), but the liver fat contents in the RG1 group were higher than those of the CG group (p<0.05). The increased hepatic collagen fibers and reticular fibers were observed in the restricted groups with the reduction of energy intake. The concentrations of nonesterified free fatty acids in the RG1 and RG2 groups were higher than those of the CG group with the reduction of energy intake (p<0.05), but there were decreased concentrations of lipoprotein lipase and hepatic lipase in both restricted groups compared with the CG group (p<0.05). In addition, the increased concentrations of β-hydroxybutyric acid, triglycerides, malondialdehyde, total antioxidant capacity and activities of superoxide dismutase activity and catalase were found in the RG1 group, and the concentrations of cholinesterase in the RG1 group were reduced compared with the CG group (p<0.05). For the concentrations of acute phase proteins, the C-reactive protein (CRP) in the RG1 group were reduced compared with the CG group, but there were no differences in haptoglobin relative to the controls (p>0.05). Conclusion: The fat accumulation, increased hepatic fibrosis, antioxidant imbalance and modified synthesis of acute phase proteins were induced in ewe’s liver by maternal malnutrition during late pregnancy, which were detrimental for liver function to accommodate pregnancy.

Hsa_circ_0070194 targets the miR-384/HDAC2 axis to enhance proliferation, cell cycle, migration and invasion of trophoblast cells

Chen Huan,Gao Cheng,Zhu Wenhui,Wan Rong,Xiong Mei 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.4

Background Preeclampsia (PE) is the most common hypertensive disorder in pregnant women. Circular RNAs (circRNAs) are a class of regulatory non-coding RNAs in human diseases, and a handful of circRNAs have been found to participate in PE regulation. The function of hsa_circ_0070194 in PE is unknown. Objective The aims of this study were to explore the biological role and regulatory mechanism of hsa_circ_0070194 in PE progression. Hsa_circ_0070194, microRNA-384 (miR-384) and histone deacetylase 2 (HDAC2) detection was performed by quantitative real-time polymerase chain reaction. Cell proliferation and cell cycle were, respectively, analyzed by Cell Counting Kit-8/colony-formation assays and flow cytometry. Cell migration and invasion were measured by wound healing and transwell assays. Western blot was carried out to determine the protein levels. The target interaction was proved via dual-luciferase reporter and RNA immunoprecipitation assays. Results Hsa_circ_0070194 level was downregulated in PE samples. HTR-8/SVneo cell proliferation, cell cycle, migration and invasion were all promoted by hsa_circ_0070194. Hsa_circ_0070194 acted as a miR-384 sponge. Hsa_circ_0070194 function was achieved partly by sponging miR-384 in HTR-8/SVneo cells. HDAC2 was a target gene of miR-384 and HDAC2 was regulated by hsa_circ_0070194 via the sponge effect on miR-384. MiR-384 targeted HDAC2 to inhibit the progression of HTR-8/SVneo cells. Conclusion This study suggested that hsa_circ_0070194 facilitated trophoblast cell proliferation, cell cycle, migration and invasion by the miR-384/HDAC2 axis. Hsa_circ_0070194 might relieve PE progression in pregnancy women.

The fabrication of aminoquinoline-functionalized polydiacetylene-based hybrid vesicles with partially reversible thermochromic property

Yue Gao,Zhiguo Zhao,Xiaojie Huang,Huan Xing,Guang Yang 한국고분자학회 2024 Macromolecular Research Vol.32 No.3

The development of smart materials with reversible thermochromic properties is of great importance in the fields of sensors, displays and anticounterfeitings. In this article, a diacetylene monomer (AQDA) containing aminoquinoline as a head group was synthesized. Together with fluorescein-substituted diacetylene (FDA) and 10,12-pentacosadiynoic acid (PCDA), hybrid PDA vesicles were obtained through self-assembly and topochemical polymerization process. The hybrid PDA vesicles (FDA, AQDA and PCDA with molar ratio of 7:2:1) display an partially reversible green-to-orange red colorimetric change in a wider temperature range from 20 to 100 °C focusing on the stronger hydrogen bonding and aromatic interactions. This work may provide fundamental understanding of the mechanism and strategies for the design of reversible thermochromic PDA materials.