In vitro Transport of Fexofenadine·HCl in Deformable Liposomes Across the Human Nasal Epithelial Cell Monolayers

Lin, Hongxia,Lee, Chi-Ho,Shim, Chang-Koo,Chung, Suk-Jae,Kim, Dae-Duk 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.6

Fexofenadine·HC1 is non-sedating histamine HI receptor antagonist that can he used for the treatment of seasonal allergic rhinitis. The objective of this study was to investigate whether the carriers of defonnable liposomes can enhance the transepithelial permeability of ferofenadine·HCl across the in vitro ALl human nasal monolay et model. Characterization of this model was achieved by bioelectric measurements and morphological studies. The passage 2 and 3 of cell monolayers exhibited the TEER value of 28521482 ohm x cm: on 11 days of seeding and maintained high TEER value for 3 days. The defonnable liposome of fexofenadine.HC1 was prepared with phosphatidylcholine (PC) and cholic acid using extruder method. The mean particle size was about 200 mn and the maximum entrapment efficiency of 33.0% was obtained in the formulation of 1% PC and 100 μg/ml ferofenadine·HC1. The toxicity of the defonnable liposome to human nasal monolayers was evaluated by MTT assay and TEER value change. MTT assay showed that it has no toxic effect on the nasal epithelial cells in 2-hour incubation when the PC concentration was below 1%. However. defonnable liposome could not enhance the transepithelial permeability (Pap,) and cellular uptake of ferofenadine·HC1. In conclusion. the in vitro model could be used in nasal drug transport studies and evaluation of transepithelial permeability of formulations.

EXPLORE THE IMPACT OF TOURIST-TO-TOURIST ON TOURISM EXPERIENCE: A SELF-DISCLOSURE PERSPECTIVE

Meng Zhang,Hongxia Lin,Xiaorong Fu 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

Human beings are intrinsically social. How social interaction among tourists affect their experience remains under-represented. Drawing on S-O-R theory, social integration theory and social penetration theory, this study proposes and empirically tests a conceptual model that integrates self-disclosure, perceived cohesion, perceived intimacy, tourist engagement and tourist satisfaction with the experience. Also, the tie strength is theorized as a moderator in the relationship between self-disclosure and perceived cohesion as well as perceived intimacy. A field experiment was undertaken to collect data. The results from the structural analysis suggest that self-disclosure influences perceived cohesion and perceived intimacy, which in turn influence their engagement and satisfaction with tourism experience. Moreover, the initial tie strength has moderation effect on the relationship between self-disclosure and perceived cohesion as well as perceived intimacy. Overall, findings of this study contributes to the development of tourism experience research from the perspective of tourist-to-tourist interaction, and broadens the research on tourist engagement. Discussions and implications for tourism operators, limitations, and suggestions for future research are also provided.

In Vitro Transport of Fexofenadine HCl in Deformable Liposomes Across the Human Nasal Epithelial Cell Monolayers

Dae-Duk Kim,Hongxia Lin,Chi-Ho Lee,Chang-Koo Shim,Suk-Jae Chung 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.6

Fexofenadine • HCl is non-sedating histamine H1 receptor antagonist that can be used for the treatment of seasonal allergic rhinitis. The objective of this study was to investigate whether the carriers of deformable liposomes can enhance the transepithelial permeability of fexofenadine • HCl across the in vitro ALI human nasal monolayer model. Characterization of this model was achieved by bioelectric measurements and morphological studies. The passage 2 and 3 of cell monolayers exhibited the TEER value of 2852482 ohm´cm2 on 11 days of seeding and maintained high TEER value for 5 days. The deformable liposome of fexofenadine • HCl was prepared with phosphatidylcholine (PC) and cholic acid using extruder method. The mean particle size was about 200 nm and the maximum entrapment efficiency of 33.0% was obtained in the formulation of 1% PC and 100 mg/ml fexofenadine • HCl. The toxicity of the deformable liposome to human nasal monolayers was evaluated by MTT assay and TEER value change. MTT assay showed that it has no toxic effect on the nasal epithelial cells in 2-hour incubation when the PC concentration was below 1%. However, deformable liposome could not enhance the transepithelial permeability (Papp) and cellular uptake of fexofenadine • HCl. In conclusion, the in vitro model could be used in nasal drug transport studies and evaluation of transepithelial permeability of formulations.

Application of biopharmaceutics classification system (BCS) in drug transport studies across human respiratory epithelial cell monolayers

조현종,Prabagar Balakrishnan,Hongxia Lin,Min-Koo Choi,김대덕 한국약제학회 2012 Journal of Pharmaceutical Investigation Vol.42 No.3

Transport studies of model drugs were conducted across the human nasal epithelial (HNE) and normal human bronchial epithelial (NHBE) cell monolayers cultured by air–liquid interface method. Physicochemical properties (e.g., molecular weight, calculated partition coefficient, dose number) of model drugs were quoted from literatures and apparent permeability coefficients (Papp)across the HNE and NHBE cell monolayers were directly measured. A linear relationship was observed between the Papp values of model drugs in the HNE and NHBE cell monolayers. As the molecular weight of model drugs increased, the Papp showed a decreasing pattern while the increase of partition coefficients resulted in the increment of Papp. These results indicated that the transport of model drugs across both cell monolayers followed mainly the passive diffusion mechanism, although substrates mediated by drug transporters showed a deviating pattern. It was also interesting to note that almost all model drugs could be grouped into the same biopharmaceutics classification system as that classified by the human intestinal permeability when the Papp was plotted as a function of dose number (D0) of each drug.

Deformable Liposomes for Topical Skin Delivery of Arbutin

김대덕,Shengjie Bian,최민구,Hongxia Lin,Junmin Zheng,정석재,심창구 한국약제학회 2006 Journal of Pharmaceutical Investigation Vol.36 No.5

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Association of MMP-9 C-1562T polymorphism with risk of Henoch-Schönlein purpura nephritis in children of southeast China

Jinwen Xu,Liping Zhao,Hongxia Zhou,Tingting Ge,Lin Zhang 한국유전학회 2017 Genes & Genomics Vol.39 No.4

Henoch-Schönlein purpura nephritis (HSPN) is the most prevalent vasculitis in childhood worldwide. Although the etiology of HSPN is not yet fully elucidated, it is believed to be closely related with infections and genetic predispositions. In this study, a cohort of children from southeast China, including 108 healthy controls and 184 patients were involved in. We scanned the promoter region and the whole gene for genetic variations. A C/T transition located in the promoter region of MMP-9 gene, C-1562T was identified. Subjects were genotyped by using SNaPshot sequencing and the frequencies of CC, CT and TT genotypes were 43, 33 and 24% in patients and 53, 37 and 10% in healthy controls. Allele distribution was found to be in Hardy–Weinberg equilibrium (P = 0.17). Association analysis revealed that the polymorphism was significantly related with HSPN risk (P < 0.05) and T allele was shown to be a risk factor (P = 0.003, odds ratio (OR) 1.63, 95% confidence interval (CI) 1.13–2.34). Additionally, we measured the serum MMP-9 concentration and quantified the mRNA expression. Results showed that an elevated protein level was observed in patients compared with controls in both ELISA (P = 0.0029, 136.5 μg/L vs. 103.9 μg/L) and Western blot assays. Furthermore, we also detected 1.93-fold increase in the mRNA transcription in patients carrying homozygous TT genotype, indicating enhanced promoter activity caused by the polymorphism. Based on the incidence risk, a cut-off value of serum MMP-9 served as HSPN diagnosis was suggested at 155 μg/L. Conclusively, our findings suggested that MMP-9 polymorphism was significantly associated with HSPN susceptibility, and C-1562T T allele contributed to HSPN development.

Protection of the hematopoietic system against radiation-induced damage: drugs, mechanisms, and developments

Yuanyun Wei,Yaqi Gong,Shuang Wei,Yonglin Chen,Jian Cui,Xiang Lin,Yueqiu Yu,Hongxia Yan,Hui Qin,Lan Yi 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.8

Sometimes, people can be exposed to moderateor high doses of radiation accidentally or through the environment. Radiation can cause great harm to several systemswithin organisms, especially the hematopoietic system. Severaltypes of drugs protect the hematopoietic system againstradiation damage in diff erent ways. They can be classifi edas “synthetic drugs” and “natural compounds.” Their cellularmechanisms to protect organisms from radiation damageinclude free radical-scavenging, anti-oxidation, reducinggenotoxicity and apoptosis, and alleviating suppression ofthe bone marrow. These topics have been reviewed to providenew ideas for the development and research of drugsalleviating radiation-induced damage to the hematopoieticsystem.

Liposome Formulation of Paclitaxel with Enhanced Solubility and Stability

Yang, Tao,Cui, Fu-De,Choi, Min-Koo,Lin, Hongxia,Chung, Suk-Jae,Shim, Chang-Koo,Kim, Dae-Duk Informa Healthcare 2007 DRUG DELIVERY Vol.14 No.5

<P> Despite its strong antitumor activity, paclitaxel (Taxol®) has limited clinical applications due to its low aqueous solubility and hypersensitivity caused by Cremophor® EL and ethanol which is the vehicle used in the current commercial product. In an attempt to develop a pharmaceutically acceptable formulation that could replace Taxol®, a paclitaxel incorporated liposome has been constructed to improve solubility and physicochemical stability. The effect of various components of the liposome, including cholesterol and lipid, on the solubility and entrapment efficiency (EE) of paclitaxel was systematically investigated. The results showed that 5% (v/v) of polyethylene glycol 400 in the hydration medium of liposome significantly increased the solubility (up to 3.39 mg/mL) as well as the EE and the paclitaxel content in the liposome formulation composed of 10% (w/v) of S100PC with cholesterol (cholesterol-to-lipid molar ratio = 10:90). When sucrose (sugar-to-lipid molar ratio = 2.3) was added as a lyoprotectant during the freeze-drying of the liposome, physicochemical stability of liposome was significantly improved. Moreover, the cytotoxicity of the final liposome formulation against MDA-MB-231 human breast cancer cell line was not significantly different from that of Taxol®. The enhanced aqueous solubility as well as the physicochemical stability of paclitaxel in the liposome formulation developed in this study could be a safer and effective alternative to the Cremophor® EL and ethanol formulation.</P>

Transport of anti-allergic drugs across the passage cultured human nasal epithelial cell monolayer

Kim Dae-Duk,Lee Chi-Ho,Yoo Jin-Wook,Lin Hongxia 大韓藥學會 2003 大韓藥學會 總會 및 學術大會 Vol.2003 No.1

Family History of Cancer and Head and Neck Cancer Risk in a Chinese Population

Huang, Yu-Hui Jenny,Lee, Yuan-Chin Amy,Li, Qian,Chen, Chien-Jen,Hsu, Wan-Lun,Lou, Pen-Jen,Zhu, Cairong,Pan, Jian,Shen, Hongbing,Ma, Hongxia,Cai, Lin,He, Baochang,Wang, Yu,Zhou, Xiaoyan,Ji, Qinghai,Zho Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Background: The aim of this study was to investigate whether family history of cancer is associated with head and neck cancer risk in a Chinese population. Materials and Methods: This case-control study included 921 cases and 806 controls. Recruitment was from December 2010 to January 2015 in eight centers in East Asia. Controls were matched to cases with reference to sex, 5-year age group, ethnicity, and residence area at each of the centers. Results: We observed an increased risk of head and neck cancer due to first degree family history of head and neck cancer, but after adjustment for tobacco smoking, alcohol drinking and betel quid chewing the association was no longer apparent. The adjusted OR were 1.10 (95% CI=0.80-1.50) for family history of tobacco-related cancer and 0.96 (95%CI=0.75-1.24) for family history of any cancer with adjustment for tobacco, betel quid and alcohol habits. The ORs for having a first-degree relative with HNC were higher in all tobacco/alcohol subgroups. Conclusions: We did not observe a strong association between family history of head and neck cancer and head and neck cancer risk after taking into account lifestyle factors. Our study suggests that an increased risk due to family history of head and neck cancer may be due to shared risk factors. Further studies may be needed to assess the lifestyle factors of the relatives.