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Hongbo Li,Yanhua Cui,Lanwei Zhang,Lili Zhang,Hui Liu,Jinghua Yu 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3
The requirements for the production of optimized Zea mays transglutaminase (TGZo) using Pichia pastoris GS115 (pPIC9K-tgzo) were optimized in this study. Plackett–Burman design was used to screen variables that significantly influence TGZo production. Oleic acid, methanol, and loading volume were identified as the most significant parameters. Central composite design was employed to determine the optimal level of these three parameters for TGZo production. Results showed that 1078 mU/mL of TGZo activity and 7.6 mg/L of TGZo production were obtained under conditions of 0.07% oleic acid, 1.31% methanol, and 7.36% loading volume. To explore the functional characteristics of TGZo, it was used in yogurt. It was found that the addition of TGZo could produce yogurt with stronger acid gel and higher consistency, cohesiveness, index of viscosity, and apparent viscosity than the untreated product. Therefore, TGZo can be used as a substitute for microbial transglutaminase in the yogurt, even in the food industry.
Zhang Hongbo,Gao Wei 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.11
Based on the big data and complexity in the identification process of IOT radio frequency, this article puts forward an optimal algorithm for Adaptive Gaussian Traversal Harmony Search IOT radio frequency identification, which aim at solving the problems of high computational complexity and low identification accuracy of traditional algorithm. At first, the network optimization design is carried out based on Harmony Search (HS) algorithm, and in order to solve the problems of standard HS in optimization accuracy, computational complexity and other aspects, control parameters are introduced into the algorithm improvisation process on the basis of traverse characteristics of Gaussian function, so that the pertinence of preliminary and later searches are improved, and meanwhile, theoretical analysis on parameters selection is described; Secondly, the optimization model of IOT radio frequency identification is studied and improved adaptive optimization objectives are put forward to achieve equilibrium optimization of performance indicators; at last, test results are compared to prove the suggested algorithm having high computational accuracy and faster computational speed.
Chirality detection of amino acid enantiomers by organic electrochemical transistor
Zhang, Lijun,Wang, Guiheng,Xiong, Can,Zheng, Lei,He, Jianbo,Ding, Yunsheng,Lu, Hongbo,Zhang, Guobing,Cho, Kilwon,Qiu, Longzhen Elsevier 2018 Biosensors & bioelectronics Vol.105 No.-
<P><B>Abstract</B></P> <P>Chiral recognition of α-amino acids is attracting increasing interest due to the importance of α-amino acids in protein metabolism as well as in food products and pharmaceuticals. Organic electrochemical transistors (OECTs) with gate electrodes modified with molecularly imprinted polymer (MIP) films were fabricated and successfully used as highly selective and sensitive chiral recognition biosensors for <SMALL>D/L</SMALL>-tryptophan (<SMALL>D</SMALL> <SMALL>/</SMALL> <SMALL>L</SMALL>-Trp) and <SMALL>D/L</SMALL>-tyrosine (<SMALL>D</SMALL> <SMALL>/</SMALL> <SMALL>L</SMALL>-Tyr). The MIP films, which can specifically recognize and has an electrocatalytic effect on the oxidation of Trp and Tyr, together with the amplification function of an OECT, provide a highly sensitive and selective OECT biosensor. The sensor showed a linear response range for <SMALL>L</SMALL>-Trp and <SMALL>L</SMALL>-Tyr from 300 nM to 10 μM with a sensitivity of 3.19 and 3.64 μA/μM, respectivity. And the detection limit for <SMALL>L</SMALL>-Trp and <SMALL>L</SMALL>-Tyr is of 2 nM and 30 nM (S/N > 3). The selectivity factors of <SMALL>L</SMALL>-Trp, <SMALL>D</SMALL>-Trp, <SMALL>L</SMALL>-Tyr and <SMALL>D</SMALL>-Tyr to their enantiomers are 11.6, 3.5, 14.5 and 2.6, respectively. This method can pave the way for widespread applications of OECT-based sensors in chiral material identification.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An novel chiral recognition MIP-OECT sensor was developed. </LI> <LI> The MIP films showed good performance of selective and electro-catalytic oxidation for Trp and Tyr. </LI> <LI> The MIP films together with the amplification function of an OECT afforded a highly sensitive, selective sensor for chirality detection of amino acid enantiomers. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Heterologous Expression and Purification of Zea mays Transglutaminase in Pichia pastoris
Hongbo Li,Yanhua Cui,Lanwei Zhang,Huaxi Yi,Xue Han,Yuehua Jiao,Ming Du,Rongbo Fan,Shuang Zhang 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5
Transglutaminases (TGases) are a family ofenzymes that catalyze the cross-linking of proteins and arewidely used in the food industry to improve the texture ofdairy, meat, and bread products. Zea mays transglutaminase(TGZ) is a new type of TGase with a wide potential. TGZwas expressed in the yeast Pichia pastoris under an alcoholoxidase promoter. Maximal expression of recombinantTGZ was achieved by inducing recombinant GS115(pPIC9K-tgz) in BMMY medium using 1.5% methanol for96 h. Secreted TGZ was initially separated using Superdex200 resin and further purified on cation exchange resin. The activity of TGZ following purification was 0.32 U/mgof protein. The polymerization effect of TGZ on caseincatalyzed by recombinant TGZ was slightly lower than theeffect of microbial transglutaminase (MTG). TGZ is a newpotential additive for the food industry.
Asymmetrical SPWM‑based control scheme for BESS‑integrated UPQC with hybrid connected configuration
Zhenqiang Zhang,Jianjun Zhao,Shen Liu,Hongbo Wang,Nan Wang,Peng Zhang,Yue Yu,Peng Xu 전력전자학회 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.10
The unified power quality conditioner (UPQC) integrated with the battery energy storage system not only mitigates the power quality problems on the grid side and load side but also supplies active power to the load or the grid. The wide voltage variation of the battery and the high efficiency of the whole system can be achieved with the hybrid connected configuration, i.e., the battery is connected simultaneously to the series and shunt converters of the UPQC directly and indirectly through the dc–dc converter. However, the control and modulation strategies are the major challenges for this configuration. This study proposes a control scheme based on asymmetrical sinusoidal pulse width modulation (SPWM) to realize high-efficiency active power delivery, thereby addressing the issue above. The mechanism of the modulation scheme to regulate active power is revealed, and the voltage variation of the battery is considered. The modified SPWM strategy features easy implementation and a low computation burden. The effectiveness of the system with the proposed control and modulation schemes is verified by both simulation and experimental results.
Zhang, Guobing,Li, Peng,Tang, Longxiang,Ma, Jingxuan,Wang, Xianghua,Lu, Hongbo,Kang, Boseok,Cho, Kilwon,Qiu, Longzhen The Royal Society of Chemistry 2014 Chemical communications Vol.50 No.24
<P>A bis(2-oxoindolin-3-ylidene)-benzodifuran-dione (BIBDF)-based low band gap polymer (PBIBDF-BT), containing a solubilizing alkyl chain bithiophene unit as a donor, has been synthesized. The polymer with a low-lying LUMO/HOMO energy level (−4.03/−5.55 eV) exhibits efficient ambipolar charge transport. The electron and hole mobilities are as high as 1.08 and 0.30 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>, respectively.</P> <P>Graphic Abstract</P><P>The PBIBDF-BT polymer exhibits high ambipolar charge transport with electron and hole mobilities as high as 1.08 and 0.30 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>, respectively. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc48695h'> </P>
Hongbo Li,Yufei Tong,Jianguang Yin,Hubiao Zhang,Changyu Yan 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.1
Multi-cavity concrete-fi lled steel tube special shaped column (CFSTSSC) combines the excellent characteristics of multicavity steel tube and core concrete. CFSTSSC has the advantages of high bearing capacity, good ductility, and strong energy dissipation capacity. At present, accurate calculation methods for these kinds of structures are limited and research into crossshaped and L-shaped multi-cavity CFSTSSCs is not available. Therefore, the axial compression behavior of cross-shaped and L-shaped multi-cavity CFSTSSCs has been investigated, though experimental research and numerical simulation, in this study. First, axial compression tests were carried out on three cross-shaped and three L-shaped multi-cavity CFSTSSCs to analyze their failure modes, axial load-strain curve, ductility index, and ultimate bearing capacity. Then, fi nite element (FE) calculation models of cross-shaped and L-shaped multi-cavity CFSTSSCs were established. The FE models are in good agreement with the experimental results, which provides a foundation for further parameter analysis and failure mechanism study of special shaped columns. Finally, combining parameter analysis and limit equilibrium theory, equations for calculating the ultimate bearing capacity of cross-shaped and L-shaped multi-cavity CFSTSSCs were proposed. The results show that the error between the simplifi ed equation and the FE result is less than 15%, indicating that the equations can provide reference for practical engineering applications.
Active DC-Link Power Filter for Single Phase PWM Rectifiers
Hongbo Li,Kai Zhang,Hui Zhao 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5
Single-phase PWM rectifier produces second-order ripple power and voltage pulsation on DC side, which is harmful to both the grid and load. The pulsating power is usually absorbed by a bulky capacitor bank and/or a dedicative LC resonant link, which results in low power density. An alternative way to do this is using some active filter circuit to direct the pulsating power to an energy-storage component. As a result, the main DC link capacitor can be reduced substantially. Based on a comparison of DC-link active power filter (APF) schemes, this paper chooses a simple but effective half-bridge topology, and proposed a control strategy based on dual-loop deadbeat control plus repetitive control. This topology with the proposed control strategy can effectively reduce the voltage pulsation on DC bus and improve the power density of the rectifier.