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Chijioke Nwoye Eze,Hideki Aoyagi,James Chukwuma Ogbonna 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.9
Production of biofuel by microalgae can become economically viable if other high-value commercial products such as carotenoid are simultaneously produced. This study aimed at enhancement of lipid and carotenoid productivity by an oleaginous microalga sp. Desmodesmus subspicatus LC172266 by supplementing Nitrogen sources to BG 11 medium under batch or fed batch mixotrophic condition. Optimum urea supplement (0.2 gL1 ) to BG 11 medium enhanced carotenoid content of the D. subspicatus by about 3.7 fold (3.02±0.002 mgg1 cell) and 3.0 fold (3.86±0.001 mgg1 cell) using glucose or glycerol as organic carbon sources, respectively. Also, lipid productivity was enhanced by about 2.0 (0.07817±0.0028 gL1 day1 ) (0.265±0.006 gg1 cell) and 2.5 (0.08426±0.0008 gL1 day1 ) (0.285± 0.02 gg1 cell) fold. Fed-batch cultivation with glucose at a feed rate of 0.5 gL1 per day enhanced both carotenoid and lipid productivities of D. subspicatus by 34% and 45%, respectively, after eight days of mixotrophic cultivation.
Activities for Information Ethics by Citizen and Students to protect Safety and Security of Children
Kazuhiro Sumi,Makoto Junnai,Hideki Yokoo,Nobuko Ekimoto,Tatsuta Aoyagi,Noriaki Nakashima,Sinngo Nozaki,Katunori Nozaki,Takatoshi Nakamura 한국콘텐츠학회 2012 ICCC International Digital Design Invitation Exhib Vol.2012 No.12
( Nwagu Tochukwu N. ),( Bartho N. Okolo ),( Hideki Aoyagi ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.5
Raw-starch-digesting enzyme (RSDA) was immobilized on Amberlite beads by conjugation of glutaraldehyde/ polyglutaraldehyde (PG)-activated beads or by crosslinking. The effect of immobilization on enzyme stability and catalytic efficiency was evaluated. Immobilization conditions greatly influenced the immobilization efficiency. Optimum pH values shifted from pH 5 to 6 for spontaneous crosslinking and sequential crosslinking, to pH 6-8 for RSDA covalently attached on polyglutaraldehyde-activated Amberlite beads, and to pH 7 for RSDA on glutaraldehyde-activated Amberlite. RSDA on glutaraldehyde-activated Amberlite beads had no loss of activity after 2 h storage at pH 9; enzyme on PG-activated beads lost 9%, whereas soluble enzyme lost 65% of its initial activity. Soluble enzyme lost 50% initial activity after 3 h incubation at 60oC, whereas glutaraldehyde-activated derivative lost only 7.7% initial activity. RSDA derivatives retained over 90% activity after 10 batch reuse at 40oC. The apparent Km of the enzyme reduced from 0.35 mg/ml to 0.32 mg/ml for RSDA on glutaraldehyde-activated RSDA but increased to 0.42 mg/ml for the PG-activated RSDA derivative. Covalent immobilization on glutaraldehyde Amberlite beads was most stable and promises to address the instability and contamination issues that impede the industrial use of RSDAs. Moreover, the cheap, porous, and non-toxic nature of Amberlite, ease of immobilization, and high yield make it more interesting for the immobilization of this enzyme.
Association of a polymorphism of BTN2A1 with dyslipidemia in East Asian populations
FUJIMAKI, TETSUO,KATO, KIMIHIKO,OGURI, MITSUTOSHI,YOHIDA, TETSURO,HORIBE, HIDEKI,YOKOI, KIYOSHI,WATANABE, SACHIRO,SATOH, KEI,AOYAGI, YUKITOSHI,TANAKA, MASASHI,YOSHIDA, HIROTO,SHINKAI, SHOJI,NOZAWA, YO Spandidos Publications 2011 Experimental and therapeutic medicine Vol.2 No.4