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Reconstituting Human Cutaneous Regeneration in Humanized Mice under Endothelial Cell Therapy
Yang, Heung-Mo,Choi, Jong-Jin,Kim, Ha-Na,Yang, Seung Jip,Park, Soon-Jung,Kang, Changhee,Chung, Hyung-Min,Lee, Man Ryul,Kim, Sung Joo,Moon, Sung-Hwan Elsevier 2019 The Journal of investigative dermatology Vol.139 No.3
Yang, Heung-Mo,Do, Hyun-Jin,Oh, Jong-Hyun,Kim, Jin-Hoi,Choi, Sang-Yun,Cha, Kwang-Yul,Chung, Hyung-Min,Kim, Jae-Hwan Wiley Subscription Services, Inc., A Wiley Company 2005 Journal of cellular biochemistry Vol.96 No.4
<P>Octamer-binding transcription factor-4 (Oct4), a member of the POU domain transcription factors, is crucial for both early embryonic development and the maintenance of stem cell pluripotency. The human Oct4 (hOct4) 5′ upstream sequence contains four conserved regions (CR1, 2, 3, 4) that are homologous in the murine. In this study, we constructed a series of deletion mutants of the hOct4 5′ upstream region and identified cis-regulatory elements that may be important determinants for the transcriptional activity of the hOct4 promoter. Our studies showed that CR2, 3, and 4 each acted as positive cis-regulatory elements in hOct4 promoter activity. We also newly identified a putative negative cis-acting element located between CR1 and CR2. In addition, the sequence −380/−1 at CR1 that contains a GC box was sufficient to provide the minimal promoter activity. Site-directed mutagenesis and electrophoretic mobility shift assays revealed the GC box located in the −380/−1 region may play a critical role in controlling the transcriptional activity of hOct4 by the direct binding of Sp1 or Sp3 transcription factors to the GC box. An overexpression study showed that Sp1 and Sp3 positively and negatively regulate hOct4 promoter activity. Thus, the hOct4 promoter upstream region contains multiple regulatory elements, one of which, the GC box, may be an important cis-regulatory element that regulates the transcription of the hOct4 promoter by the binding of Sp family transcription factors. © 2005 Wiley-Liss, Inc.</P>
Transcriptional regulation of human Oct4 by steroidogenic factor-1
Yang, Heung-Mo,Do, Hyun-Jin,Kim, Dong-Ku,Park, Jin-Ki,Chang, Won-Kyong,Chung, Hyung-Min,Choi, Sang-Yun,Kim, Jae-Hwan Wiley Subscription Services, Inc., A Wiley Company 2007 Journal of cellular biochemistry Vol.101 No.5
<P>Oct4 encodes a transcription factor that is involved in the maintenance of self-renewal in stem cells. Recently, the molecular mechanisms that regulate Oct4 expression have come under investigation. In this study, we demonstrate that the orphan nuclear receptor steroidogenic factor-1 (SF-1) behaves as a transcriptional activator of human Oct4 (hOct4) through direct interaction with a SF-1 binding element in the hOct4 proximal promoter. We found that Oct4 and SF-1 were co-expressed in undifferentiated human embryonal carcinoma NCCIT cells and downregulated during retinoic acid-mediated differentiation. We examined the functional role played by SF-1 in regulation of hOct4 transcription using a luciferase reporter assay and Western blot analysis. Overexpression of SF-1 increased up to about threefold hOct4 promoter activity and endogenous hOct4 protein expression. Sequence analysis of the hOct4 promoter revealed that the transcriptional activity was closely linked to Conserved Regions 1 (CR1) and 2 (CR2), which contain three putative SF-1-binding sites (1st, 2nd, and 3rd SF-1). Binding assays and mutagenesis of binding sites indicated that the 1st and 2nd SF-1 elements (in CR1 and CR2, respectively) might be important cis-regulatory elements in hOct4 promoter activity. However, differences in response to SF-1 overexpression between wild-type and mutant hOct4 promoters revealed that the 1st SF-1 element is the key binding site for SF-1-mediated transcriptional activation. Thus, our data indicate that SF-1 plays a crucial role in the regulation of hOct4 transcription through direct binding to the 1st SF-1 in CR1 of the hOct4 proximal promoter. J. Cell. Biochem. 101:1198–1209, 2007. © 2007 Wiley-Liss, Inc.</P>