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RISS 인기검색어
- 검색키워드
- 저자 : HaeyoungSuh-Kim (검색결과 3 건)
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Inhibition of BETA2/NeuroD by ld2
길승호,전용진,HaeyoungSuh-Kim 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.5
Inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, known as statins, are widely used for primary and secondary prevention atherosclerosis is multistep processes where trans-endothelial migration of various leukocytes includ-ing monocytes is a crucial step. Interferon-γ (IFN-γ) contributes in this process by activating macro-phages and T-lymphocytes, and by inducing adhe-sion molecules in vascular endothelial and smooth muscle cells. In this study we investigated the expresion of intercellular cell adhesion mole-cule-1 (ICAM-1) in transformed endothelial cell line ECV304 cells as influenced by lovastatin, tumor necrosis factor-α (TNF-α) and IFN-γ. Results show that lovastatin suppresses expression of ICAM-1 by inhibiting the IFN-γ-induced extracellular signal- regulated kinase (ERK) p44/p42-STAT1 signaling pathway. In cells treated with lovastatin and IFN-γ, ICAM-1 was expressed at a lower level than in cells treated with IFN-γ alone. However, lovastatin does not reduce TNF-α induced expression of ICAM-1. A similar result was observed in cells N-γ. Cis-acting DNA sequence elements were identified in the 5'-flanking region of the ICAM-1 promoter that mediate inhibition by lovastatin; these se-quences map to the IFN-γ activated site which also binds the STAT1 homodimer. However, lovastatin did not inhibit IFN-γ-mediated induction of the Y701 phosphorylated form of STAT1. But lovasta-tin does inhibit the IFN-γ-mediated phosphoryla-tion of ERK1/ERK2 (T202/Y204) and S727 phos-phorylation of STAT1. TNF-αphosphorylation of ERK1/ERK2 and S727 in ECV304 and smooth muscle cels. The results provide the evidences that statins may have beneficial effects by inhibiting IFN-γ action in atherosclerotic pro-cess
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Overexpression of neurogenin1 induces neurite outgrowth in F11 neuroblastoma cells
김소연,길성호,김성수,명현호,이영돈,HaeyoungSuh-Kim 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.6
Neurogenin1 (Ngn1) is a basic helix-loop-helix (bHLH) transcription factor expresed in neuronal precursors in the developing nervous system. The function of Ngn1 in neurogenesis has been shown in various aspects. In this study, we investigated the neurogenic potential of Ngn1 using neuro-blastoma cel line, F11, which could be induced to diferentiate into neurons in the presence of cAMP. To investigate the expresion of Ngn1, ex-pression vectors for the ful-length and the C- ted and their transactivation potential was verified using reporter gene containing the E-box se-quence. Overexpression of the ful-length Ngn1 induced neurite outgrowth in F1 cells in the ab-sence of cAMP. A C-terminal deletion mutant, Ngn1(1-197), inhibited neurite outgrowth induced by cAMP in F11 cells. These results indicate that the Ngn1 plays an important role in diferentiation of neuroblastoma cels and the C terminus of Ngn1 is essential for the efficient diferentiation.
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Sun-JungCho,JaeSeobJung,SeungChulShin,IngNyolJin,BokHyunKo,YunheeKimKwon,HaeyoungSuh-Kim,문일수 생화학분자생물학회 2003 Experimental and molecular medicine Vol.35 No.6
The 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), a protein of unknown function in vivo, is abundantly expresed in myelinating glia in two isoforms, CNP1 and CNP2. In this study, imu-noblot analysis showed that CNP1 is the major isoform in adult forebrain, and that both isoforms are included in the postsynaptic density (PSD) fraction and tyrosine-phosphorylated at the basal level. However, subcellular distribution and deter-gent extraction data showed that CNP is nonspeci-ficaly associated with the PSD fraction. Imuno-cytochemistry revealed that CNP is detected, in a weak but punctate patern, in disociated rat hipo-campal neurons of 3 days to 2 weeks in vitro. The CNP-positive punctae were distributed throughout soma and dendrites, and distinct from PSD95-posi-tive ones. Imunoblot analysis indicated that CNP and F11. Interestingly, in addition to the known two isoforms, a new CNP isoform of MW 45 kDa was expresed in these cel lines and was the ma-jor type of isoform in F1 cells. Taken together, our data sugest that CNP is expressed in the ear-ly stage of in vitro development and nonspeci-ficaly included in the adult rat PSD fraction.
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