Expression of PGDH Correlates with Cell Growth in Both Esophageal Squamous Cell Carcinoma and Adenocarcinoma

Yang, Guo-Tao,Wang, Juan,Xu, Tong-Zhen,Sun, Xue-Fei,Luan, Zi-Ying Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Esophageal cancer represents the fourth most common gastrointestinal cancer and generally confers a poor prognosis. Prostaglandin-producing cyclo-oxygenase has been implicated in the pathogenesis of esophageal cancer growth. Here we report that prostaglandin dehydrogenase, the major enzyme responsible for prostaglandin degradation, is significantly reduced in expression in esophageal cancer in comparison to normal esophageal tissue. Reconstitution of PGDH expression in esophageal cancer cells suppresses cancer cell growth, at least in part through preventing cell proliferation and promoting cell apoptosis. The tumor suppressive role of PGDH applies equally to both squamous cell carcinoma and adenocarcinoma, which enriches our understanding of the pathogenesis of esophageal cancer and may provide an important therapeutic target.

Performance study of g-C3N4/carbon black/BiOBr@Ti3C2/MoS2 photocatalytic fuel cell for the synergistic degradation of different types of pollutants

Guo Huilin,Yu Tingting,Zhao Lei,Qian Jun,Yu Jiahe,Zhang Yu,Teng Yongyue,Zhu Chunshui,Yang Tao,Chen Wenbin,Gong Picheng,Jiang Cuishuang,Gao Changfei,Yang Bing,Yang Chenyu 한국탄소학회 2023 Carbon Letters Vol.33 No.3

In this study, a bipolar visible light responsive photocatalytic fuel cell (PFC) was constructed by loading a Z-scheme g-C3N4/carbon black/BiOBr and a Ti3C2/MoS2 Schottky heterojunction on the carbon brush to prepare the photoanode and photocathode, respectively. It greatly improved the electron transfer and achieved efficient degradation of organic pollutants such as antibiotics and dyes simultaneously in two chambers of the PFC system. The Z-scheme g-C3N4/carbon black/BiOBr formed by adding highly conductive carbon black to g-C3N4/BiOBr not only effectively separates the photogenerated carriers, but also simultaneously retains the high reduction of the conduction band of g-C3N4 and the high oxidation of the valence band of BiOBr, improving the photocatalytic performance. The exceptional performance of Ti3C2/MoS2 Schottky heterojunction originated from the superior electrical conductivity of Ti3C2 MXene, which facilitated the separation of photogenerated electron–hole pairs. Meanwhile, the synergistic effect of the two photoelectrodes further improved the photocatalytic performance of the PFC system, with degradation rates of 90.9% and 99.9% for 50 mg L−1 tetracycline hydrochloride (TCH) and 50 mg L−1 rhodamine-B (RhB), respectively, within 180 min. In addition, it was found that the PFC also exhibited excellent pollutant degradation rates under dark conditions (79.7%, TCH and 97.9%, RhB). This novel pollutant degradation system is expected to provide a new idea for efficient degradation of multiple pollutant simultaneously even in the dark.

Correlations of Four Genetic Single Nucleotide Polymorphisms in Brain-Derived Neurotrophic Factor with Posttraumatic Stress Disorder

JunCheng Guo,YiJun Yang,Min Guo,XiaoDan Wang,Yang Juan,YunSuo Gao,LinQiu Fu,XiangLing Jiang,LinMei Fu,Tao Huang 대한신경정신의학회 2018 PSYCHIATRY INVESTIGATION Vol.15 No.4

Objective-To investigate the correlations of four genetic single nucleotide polymorphisms (SNPs) of brain-derived neurotrophic factor (BDNF) with posttraumatic stress disorder (PTSD). Methods-A total of 300 patients with sporadic PTSD and 150 healthy subjects (the control group) were selected according to the diagnostic criteria of PTSD (DSM-IV), and the genotypes of the BDNF SNPs G-712A, C270T, rs6265, and rs7103411 were detected by polymerase chain reaction and direct DNA sequencing to determine intergroup differences in the genotypes and allele frequencies; the pvalues were corrected with the permutation test. Results-The genotypes and allele frequencies of the SNPs G-712A, rs6265, and rs7103411 of BDNF showed no significant intergroup differences (p>0.05). However, the genotype and allele frequencies of C270T showed significant differences between the PTSD group and the control group (p<0.05). Conclusion-The SNP C270T of BDNF may be associated with PTSD. Individuals carrying the polymorphic T allele of C270T may be more likely to suffer from PTSD.

Isochaetomium A2, a new bis(naphthodihydropyran-4-one) with antimicrobial and immunological activities from fungus Chaetomium microcephalum

Guo-Bo Xu,Tao Yang,Jin-ku Bao,Dong-Mei Fang,Guo-You Li 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

Isochaetomium A2 (1), a new bis(naphthodihydropyran-4-one), along with chaetochromins A (2) and B(3), was isolated from the solid-state fermented rice cultureof Chaetomium microcephalum. The structure of compound1 was elucidated on the basis of 1D and 2D NMRspectral data, and the relative configuration was confirmedby CD spectrum. Compounds 1–3 possessed significantantimicrobial activity against Escherichia coli 1.044,Staphylococcus aureus 1.252, and Bacillus subtilis 1.079. Moreover, compounds 1–3 showed obvious inhibitoryeffects on mouse spleen cell proliferation with successiveIC50 values of 0.52, 0.19, and 0.24 lM.

Behavior and simplified analysis of steel-concrete composite beams subjected to localized blast loading

Guo-Qiang Li,Su-Wen Chen,Tao-Chun Yang 국제구조공학회 2009 Structural Engineering and Mechanics, An Int'l Jou Vol.32 No.2

Finite element simulations are increasingly used in structural analysis and design, especially in cases where complex structural and loading conditions are involved. Due to considerable progresses in computer technology as well as nonlinear finite-element analysis techniques in past years, it has become possible to pursue an accurate analysis of the complex blast-induced structural effects by means of numerical simulations. This paper aims to develop a better understanding of the behavior of steel-concrete composite beams (SCCB) under localized blast loading through a numerical parametric study. A finite element model is set up to simulate the blast-resistant features of SCCB using the transient dynamic analysis software LS-DYNA. It is demonstrated that there are three dominant failure modes for SCCB subjected to localized blast loading. The effect of loading position on the behavior of SCCB is also investigated. Finally, a simplified model is proposed for assessing the overall response of SCCB subjected to localized blast loading.

Effects of metakaolin and PVA fiber on the compressive strength of shotcrete under different curing conditions

Yang Guoqiang,Guo Gang,Wang Lihui,Wang Changchang,Sun Tao 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.3

In this paper, wet shotcrete spraying experiments were carried out under different temperature and humidity conditions withdifferent dosage of metakaolin (MK) and different length of polyvinyl alcohol fiber (PVA fiber). And the influence of differentcontent of metakaolin and different length of PVA fiber on the compressive strength of tidal shotcrete under different curingconditions was explored. The results show that the compressive strength of shotcrete increases first and then decreases withthe increase of MK content and PVA fiber length under the curing condition of normal temperature and constant humidity. However, under the curing condition of high temperature and humidity, the compressive strength of shotcrete tended toincrease with the increase of MK content. Under the condition of high temperature and high humidity curing, the compressivestrength of shotcrete is lower than that of shotcrete under normal temperature and constant humidity curing. This study canprovide theoretical support for the application of sprayed concrete in different environments.

BEFS-PPARγ2 Cells Incubated with trans-11 C18:1 Exhibit More Beneficial Fatty Acid Synthesis

Tao Wang,이홍구,LiFang Wu,GuiXin Qin,Yujie Lou,Zewei Sun,Wei Guo,Jian Yang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.5

Responses of the PPARγ2 (an adipogenic lineage determinant) over-expressed immortalized bovine embryonic fibroblast cell line (BEFS-PPARγ2 cells) to trans-11 C18:1 (TVA) incubation were evaluated. BEFS-PPARγ2 cells were well differentiated. Stearoyl-CoA desaturase 1, an endoplasmic reticulum enzyme that can catalyze conversion of TVA into cis-9, trans-11 CLA, exhibited a significantly (p<0.05) higher mRNA expression after 4 h, compared with controls. Enzyme activity peaked, and was significantly (p<0.05) higher after 6 h, compared with controls. With extension of the incubation time, TVA absorption into cells was significantly (p<0.05) higher with significantly (p<0.05) more conversion into cis-9, trans-11 CLA, compared with shorter incubation times. TVA incubated cells exhibited significantly (p<0.05) higher levels of arachidonic, eicosapentaenoic, and docosahexaenoic acids than controls. BEFS-PPARγ2 cells can be used as a model system and TVA incubation is a good approach for research into cis-9, trans-11 CLA-fortified beef production.

Fusing Minimal Unit Probability Integration Method and Optimized Quantum Annealing for Spatial Location of Coal Goafs

Tao Wei,Guangli Guo,Huaizhan Li,Lei Wang,Xiangsheng Yang,Yuezong Wang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.5

Accurately detecting the location of the goafs is an effective method for coordinating underground mining and surface engineering construction and realizing illegal mining supervision. Aiming at the problems of the existing solution methods of goaf spatial characteristic parameters, a spatial location identification method of coal underground goaf with fusing minimal unit probability integration method and optimized quantum annealing is proposed. Meanwhile, to study the characteristics and stability of the proposed model, the advantages and disadvantages of space movement vector data, the robust ability of the method, and the application of multi-source data are discussed in the Discussions. Finally, the achievements of this paper apply to 1414 (1) working face of Gubei Coal Mine in Huainan. The results show the model can accurately identify the location and boundary of the goaf. The research achievements have important theoretical and practical significance for solving problems of land resource reuse in mining areas lacking geological mining data, coal mine safety production, and the supervision of illegal mining.

A Rapid and Convenient Method for in Vivo Fluorescent Imaging of Protoscolices of Echinococcus multilocularis

Tao Yang,Sibo Wang,Xuyong Zhang,Jie Xia,Jun Guo,Jixue Hou,Hongwei Zhang,Xueling Chen,Xiangwei Wu 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.2

Human and animal alveolar echinococcosis (AE) are important helminth infections endemic in wide areas of the Northern hemisphere. Monitoring Echinococcus multilocularis viability and spread using real-time fluorescent imaging in vivo provides a fast method to evaluate the load of parasite. Here, we generated a kind of fluorescent protoscolices in vivo imaging model and utilized this model to assess the activity against E. multilocularis protoscolices of metformin (Met). Results indicated that JC-1 tagged E. multilocularis can be reliably and confidently used to monitor protoscolices in vitro and in vivo. The availability of this transient in vivo fluorescent imaging of E. multilocularis protoscolices constitutes an important step toward the long term bio-imaging research of the AE-infected mouse models. In addition, this will be of great interest for further research on infection strategies and development of drugs and vaccines against E. multilocularis and other cestodes.

Effect of L-carnitine on sperm quality during liquid storage of boar semen

Yang Kang,Wang Na,Guo Hai-Tao,Wang Jing-Ran,Sun Huan-Huan,Sun Liang-Zhen,Yue Shun-Li,Zhou Jia-Bo 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage. Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooled-storage at 17°C. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated. Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential. Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17°C.