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Huabin Zhou,Gaoqiang Liu,Furu Huang,Xiangting Wu,Hailong Yang 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.12
Polysaccharides from Ganoderma lucidumshowed multiple biological activities, such as immunomodulating,antitumor, antioxidant, and hepatoprotectiveactivity, etc. Adlay oil was added into the media to enhancepolysaccharide production by submerged culture of G. lucidumin this work. The results revealed the optimal concentrationof adlay oil was 1.5 % for polysaccharideproduction of G. lucidum. Analysis of the polysaccharidecomponents confirmed that no novel components werebiosynthesized by the addition of adlay oil. The mainfraction of extracellular polysaccharide, GLEP-2, wasisolated from the submerged culture broth of G. lucidum byethanol precipitation, filtration, DEAE cellulose-52 andSepharose CL-6B chromatography. GLEP-2, which wascomposed of glucose, galactose, mannose, arabinose, andrhamnose in a ratio of 332:55:32:13:3 respectively, had anaverage molecular weight of *2.08 9 104 Da. The bioactivitytests demonstrated that GLEP-2 enhanced the Tlymphocyte proliferation significantly at a concentration of200 lg/mL and B lymphocyte proliferation at lower concentrationsof 50 lg/mL. The results suggest polysaccharidesfrom the submerged culture of G. lucidum arepotential candidates for further development and possiblecommercial applications, especially in the pharmaceuticaland functional foods industries.