http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Genome-wide association studies approach and post-GWAS study in rice
Gang Li,Min-Young Yoon,Won-Hee Ra,Jae-Wan Park,Qiang He,Aye Aye Khaing,Xiao-Qiang Wang,Win Htet Oo,Feng-Peng Li,Byoung Kook Yun,Chang-Yong Lee,Yong-Jin Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
AGenome-wide association studies (GWAS) have proven a useful technique for identifying genetic loci responsible for natural variation in rice. With the fast developed next-generation sequencing technology, it is possible for people to carry out GWAS by phenotyping different traits. However, how to make full use of huge data, abandon unnecessary data, and solve the problem of data application effectively seems still an obstacle for many researchers. Taking the case of whole-genome resequencing of Korean authentic rice core set, here we present a general technological path of GWAS including: 1) a schematic view of sequencing-based GWAS in rice; 2) a user-friendly and interactive web application for GWAS in rice by the aid of experience from Arabidopsis; 3) Haplotype and association analysis of candidate genes in a certain mechanism pathway, giving 10 starch synthesis genes as example; and 4) functional validation by Trans- and Mata-Omics analysis.
율무한방차의 p-AMPK 발현증가에 의한 C57BL/6 마우스 간조직 지질축적 억제 효과
유강완(Gang Wan Yoo),박해진(Hae-Jin Park),조수정(Su-Jung Cho),이세희(Se Hui Lee),박순애(Soon-Ae Park),김미림(Mi-Lim Kim) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.4
본 연구는 율무한방차의 간조직 지질대사 관련 단백질 발현 조절에 의한 지질축적 억제 작용을 알아보고자 하였다. C57BL/6 마우스를 4개군, 즉 Normal(정상식이군), Control(60% 고지방식이군), Control+가르시니아 캄보지아 200 mg/kg/d(GC군), Control+율무감비 열수추출물 200 mg/kg/d(YM200군)로 나누어 실험을 진행하였다. 실험 결과, 혈청 TG, TC 및 LDL-C 농도와 혈청 MDA 농도가 GC 및 YM200군에서 Control군보다 유의적으로 감소하였다. YM200 보충은 간조직 p-AMPK 발현을 증가시키고 간조직 지질합성에 주요한 역할을 하는 SREBP-2 및 HMGCR 단백질 발현을 감소시킨 반면, 간조직 지방산 산화 관련 단백질인 CPT-1 및 UCP-2 발현을 증가시킴으로써 간조직의 TG 및 TC 농도를 감소시키고 지방구 축적을 억제하는 결과를 나타내었다. 결론적으로 YM200은 간조직 p-AMPK 발현을 증가시켜 지질대사 관련 단백질 발현을 조절함으로써 간조직의 지질축적을 억제하였다. 따라서 YM200은 간조직 지질대사를 조절하고 지방간 제어에 효과적이라고 판단된다. The present study investigated the hepatic lipid-lowering effect of Yulmu herbal tea mediated by regulating hepatic protein expressions. C57BL/6 mice were randomly divided into four groups: (1) normal (normal diet), (2) control (60% high-fat diet), (3) control+Garcinia cambogia 200 mg/kg/d (GC), (4) control+Yulmugambi water extract 200 mg/kg/d (YM200). Serum triglyceride (TG), total cholesterol (TC), and low-density lipoprotein concentrations significantly decreased in the GC and YM200 groups compared to the control group. There was also a significant reduction in the serum malondialdehyde levels in the GC and YM200 groups. The YM200 treatment significantly increased the hepatic phosphorylated-AMP-activated protein kinase (p-AMPK) expression, and inhibited the hepatic sterol regulatory element-binding protein-2 and 3-hydroxy-3-methylglutaryl coenzyme A reductase protein expressions, which are the key regulators of hepatic lipid synthesis. YM200 also significantly upregulated hepatic fatty acid oxidation proteins such as carnitine palmitoyltransferase 1 and uncoupling protein 2. Therefore, hepatic TG and TC levels and lipid droplets were suppressed in the YM200 group. YM200 reduced hepatic lipid accumulation and regulated hepatic lipid-relating protein expressions mediated by upregulating the p-AMPK expression. In conclusion, YM200 may be useful for regulating hepatic lipid metabolism and hepatic steatosis.
Smartwatch-Based Wearable EEG System for Driver Drowsiness Detection
Gang Li,Boon-Leng Lee,Wan-Young Chung IEEE 2015 IEEE Sensors Journal Vol.15 No.12
<P>Driver drowsiness is a major cause of mortality in traffic accidents worldwide. Many physiological signals have been proposed to detect driver drowsiness. Among these signals, an electroencephalographic (EEG) signal, which reflects the brain activities, is more directly related to drowsiness. Thus, many EEG-based driver drowsiness detection (DDD) models gained more and more attention in recent years. However, one limitation of these studies is that these models merely estimate discrete labels and, thus, did not allow for estimating the relative severity of driver drowsiness. This paper proposes a support vector machine-based posterior probabilistic model (SVMPPM) for DDD, aimed at transforming the drowsiness level to any value of 0~1 instead of discrete labels. A fully wearable EEG system which consists of a Bluetooth-enabled EEG headband and a commercial smartwatch was used to evaluate the proposed model in a real-time way. Twenty subjects who participated in a 1-h monotonous driving simulation experiment were used to develop this model with fifteen subjects for a building model and five subjects for a testing model. According to a video-based reference, the proposed system obtained an accuracy of 91.25% for an alert group (73 out of 80 data sets), 83.78% for an early-warning group (93 out of 111 data sets), and 91.92% for a full-warning group (91 out of 99 data sets). These results indicate that the combination of the proposed SVMPPM, the EEG headband, and the wrist-worn smart device constitutes an effective, simple, and inexpensive wearable solution for DDD.</P>
Wan, Han-Feng,Zhang, Bin,Yan, Dan-Gui,Xu, Zhen-Gang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7
Background: The phenomenon of occult carcinoma maybe observed in patients with clinically unilateral papillary thyroid microcarcinoma (PTMC). Although many studies have reported that the $BRAF^{T1799A}$ mutation is associated with aggressive PTMC, the relationship between $BRAF^{T1799A}$ mutation and occult carcinoma is unclear. The aim of this study was to investigate the risk factors, including $BRAF^{T1799A}$ mutation, for occult contralateral carcinoma in clinically unilateral PTMC accompanied by benign nodules in the contralateral lobe. Materials and Methods: From January 2011 to December 2013, we prospectively enrolled 89 consecutive PTMC patients with clinically unilateral carcinoma accompanied by benign nodules in the contralateral lobe who received a total thyroidectomy and cervical lymph node dissection. $BRAF^{T1799A}$ mutation was tested by pyrosequencing on postoperative paraffin specimens. The frequency and predictive factors for occult contralateral carcinoma were analyzed with respect to the following variables: age, gender, family history, tumor size, presence of Hashimoto thyroiditis, extrathyroidal extension, central lymph node metastasis, multifocality of primary tumor, or $BRAF^{T1799A}$ mutation. Results: A total of 36 patients (40.4%) had occult PTMC in the contralateral lobe. The median diameter of the occult tumors was $0.33{\pm}0.21cm$. The $BRAF^{T1799A}$ mutation was found in 38 cases (42.7%). According to the univariate analysis, there were no significant differences between the presence of occult contralateral carcinoma and age, gender, family history, tumor size, presence of Hashimoto thyroiditis, extrathyroidal extension, central lymph node metastasis, multifocality of primary tumor, or $BRAF^{T1799A}$ mutation. Conclusions: Using current methods, it is difficult to preoperatively identify patients with PTMC, and further research is needed to determine predictive factors for the presence of occult contralateral carcinoma in patients with unilateral PTMC.