Butane gas가 흰쥐 혈청과 조직의 Lactatedehydrogenase 및 Cholinesterase에 미치는 영향

윤수홍,박은주,조수열,최현태 한국환경독성학회 1991 환경독성보건학회지 Vol.6 No.2

Acute poisoning with organic solvents and other volatile compounds now usually follows deliberate inhalation (volatile substance abuse) or ingestion of these compounds. The effect of butane gas inhalation was analyzed for serum, liver, brain, lung and muscle. And the observations are revealed on rat cholinesterase activity, lactatedehydrogenase activity and electrophoretic pattern of lactatedehydrogenase isozyme. The results are as follows: 1. The rat cholinesterase activity on serum, liver and muscle show the decreased by increasing of inhalation time of butane gas in particular the lung cholinesterase activity was greatly affected. 2. Butane gas inhalation brought out the lactatedehydrogenase activity increased of the serum and the tissues and had an important effect especially in both the liver and muscle lactatedehydrogenase activities. 3. Each tissue was found to have a characteristic distribution of lactatedehydrogenase isozymes on celluloseacetate electrophoresis and the development of inhalation time is shown the disappearance and diffusion of band. The toxicity of butane gas inhalation was most prominence in the liver and lung toxicity was occurred also.

迎日灣의 ECOSYSTEM 硏究 3. 水溫

尹伊鏞,朴相來,李東澈,金基台,崔銀珠,安由煥 嶺南大 海洋科學硏究所 1988 Marine Nature Vol.1 No.1

In order to observe hydrological characteristics in the Gulf of Yoeng-il, measurements of water temperature have been carried out from February to August 1983, and from August 1985 to December 1986. Surface water temperatures are closely linked to air temperature and solar-radiation for all littoral stations. Seasonal and annual variations of all horizontal stations are very similar to one another. Vertical variations of water temperatures for the stations located along the central-line of the Gulf are characteristic (and observed) as thermoclines and thermal stratification in the hottest summer-time (August). It is interesting to note that water column temperature of July and September are rather warmer than August, on account of a cold water mass coming from NE direction. For example, surface temperature in June 1982 was above 19℃, but the water below a depth of about 30m drastically drops to 4℃. It is indicative of invasion of water mass from cold current or deep sea.

Benzo(a) pyrene에 의해 유도된 간기능 장해에 미치는 시호와 황금의 병용 효과

尹水弘,吳官絃,朴銀珠 대구효성가톨릭대학교 1992 연구논문집 Vol.45 No.1

This study was attempt to evaluated the effect of Bupleuri Radix extract and Scutellariae Radix extract as the hepatotropic agents. In addition, we measured the relative effectiveness of drug combined treatment in comparison to that of alone treatment. The results are as follows ; 1. Serum and liver AST, ALT activities and liver bilirubin content of B(a)P treated group were significantly increased in comparison to normal group. After administration of Bupleuri Radix extract these enzyme activity and bilirubin content were decreased. 2. Scutellariae Radix extract decreased the increase of serum and liver AST, ALT, liver LDH activities and bilirubin content in B(a)) treated group. 3. After combined treatment of Bupleuri Radix and Scutellariae Radix extract, the serum and liver AST, ALT activities and liver bilirubin content were significantly decreased in comparison to that of drug alone treatment. 4. Buplerui Radix extract prevented the leakage of serum and liver total cholesterol, serum phospholipid and triglyceride levels caused by B(a)P treatment. 5. Scutellariae Radix extract prevented the leakage of serum and liver triglyceride and liver total cholesterol levels caused by B(a)P treatment. 6. After combined treatment of Bupleuri Radix and Scutellariae Radix extract, the lipid accumulation of serum and liver was significantly decreased in comparison to that of drug alone treatment. 7. The hepatotropic effect of drug extracts against B(a)P induced hepatotoxicity was superior to combined treatment of Bupleuri and Scutellariae Radix extract and after-treatment in comparison to that of alone treatment and pretreatment.

흡입물질이 흰쥐 Lactate Dehydrogenase와 Cholinesterase 활성변화에 미치는 영향

윤수홍,박병윤,하헌,박은주 한국환경독성학회 1995 환경독성보건학회지 Vol.10 No.1

The effects of volatile substances inhalation on lactate dehydrogenase and cholinesterase in rats were investigated. Male Sprague-Dawley rats were exposed to marketed odorant, ethyl acetate and ethyl ether for 15 days. Enzyme activities were measured in serum and several tissues such as liver, lung, brain, heart, kidney and muscle to find differences of effects according to the organ. Cholinesterase activity in serum and most of tissues revealed time-dependent decrease in the case of marketed odorant inhalation. Especially in heart and kidney significant decrease was observed. Ethyl acetate exposure to rats revealed also decrease in serum and all tissues by 40% to 60%. Ethyl ether inhalation showed significant decrease by 30% to 50°,%. Lactate dehydrogenase activity was markedly increased in serum and similarly in heart, brain and kidney by exposure to marketed odorant. No changes were observed in liver. Ethyl acetate exposure to rats revealed increase in serum by about 200%, compared to normal group and in other tissues by 40% to 70% except in liver and muscle. Ethyl ether inhalation showed significant increase in serum by about 100%. There was no change in liver and slight increase in muscle.

Benzo(a)pyrene에 의해 유도된 간기능장해에 미치는 자근 추출액의 영향

윤수홍,박은주,오관현,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

민간에서 주로 사용되는 약용식물들 중 간독성 의 발현 저해 및 간기능 보호 효과가 우수한약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험과정으로 탄소화합물의 불완전 연소및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의 간독성발현에 미치는 자근 수침액의 예방 및 치료 효과를 실험한 결과 자근 수침액의 투여는B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성있게 감소시켰고 B(a)P 투여로 증가한 혈청 total cholestelof 및 phospholipid 함량 역시감소시킬 수 있었으나 그 효과는 현저하지 않았다. 즉, 자근 수침액의 투여는 B(a)P에의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the phamacologicalactivities of crude Lithospermi Radix reported with the clinical uses in the orientalmedicine. Crude Lithospermi Radix uesd for the treatment of burn, eczema,blister, diuretic, scarlet fever and septicemia as antipyretic, antidotic and antiph-logistics. Therefore we tested the effects of Lithospermi Radix water extracton the liver-protective activities in the rats. The results obtained from enzymeassay, measurement of serum and liver alanine aspartate aminotransferase(ALT,AST) and lipid composition indicated that Lithospermi Radix water extract sho-wed significant liver-protective activities against benzo(a)pyrene-induced hepato-toxicity.

흡입물질의 위생학적 연구 : 본드 흡입이 흰쥐의 폐 및 기타 장기의 효소 활성에 미치는 영향 The Effect of Bond Inhalation on the Enzyme Activity of Lung and Other Organs in Rat

윤수홍,박은주,이주영,서혜령 효성여자대학교 부설 한국환경위생연구소 1991 환경위생연구 Vol.1 No.1

Toluene, alcohol및 vinylester류 등을 함유하고 있는 bond를 이용하여 휘발성 물질이 생체에미치는 영향을 관찰하기 위하여 rat에 일정 농도의 bond를 일정 시간 흡입시킨 뒤 각 조직에대하여 효소의 생화학적 시험과 celluloseacetate electrophoresis를 실시하여 다음과 같은 결과를얻었다. 1. Bond 흡입은 시간이 증가함에 따라 혈청, 심장, 신장, 근육, 뇌의 ChE 활성을 억제하였으며특히 폐와 간장의 ChE활성에 가장 큰 영향을 나타내었다. 2. Bond 흡입에 의해 혈청 및 각 조직 중에서 LDH활성이 증가되었으며 특히 폐와 심장,근육에서 그 영향이 현저히 나타났다. 3. Celluloseacetate electrophoresis를 통한 각 조직의 LDH isozyme pattern에는 차이가 나타났으며 bond 흡입에 의해 band의 소멸과 선명도의 감소가 일어 났는데 특히 폐에 대한 독성이가장 현저했으며 근육에 대한 독성도 큰 것으로 나타났다. Acute poisoning with organic solvents and other volatile compou-nds now usually follows deliberate inhalation(volatile substance abuse) or inges-tion of these compounds. The effect of bond inhalation was analyzed for serum, liver, lung, brain,heart, kidney and muscle. And the observations are revealed on rat cholinesterase activity, lactatedeh-ydrogenase activity and electrophoretic pattern of lactatedehydrogenase isozyme. The results are as follows ; 1. The rat cholinesterase activity on serum, heart, muscle, kidney and brain showthe decrease by increasing of inhalation time of bond, in particular the lungand liver cholinesterase activity was greatly affected. 2. Bond inhalation brough out the lactatedehydrogenase activity increased ofthe serum and the tissues and had an important effect especially in both thelung and muscle lactatedehydrogenase activities. 3. Each tissue was found to have a characteristic distribution of lactatedehydro-genase isozymes on celluloseacetate elelctrophoresis and the development ofinhalation time is shown the disappearence and diffusion of band.

Benzo(a)pyrene에 의해 유도된 간기능장해에 미치는 자근 추출액의 영향

윤수홍,박은주,오관현,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

민간에서 주로 사용되는 약용식물들 중 간독성의 발현 저해 및 간기능 보호효과가 우수한 약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험 과정으로 탄소화합물의 불완전 연소 및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene 으로 유도한 rats 의 간독성 발현에 미치는 자근 수침액의 예방 및 치료효과를 실험한 결과 자근 수침액의 투여는 B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성있게 감소시켰고, B(a)P투여로 증가한 혈청 total cholesterol 및 phospholipid 함량 역시 감소시킬 수 있었으나 그 효과는 현저하지 않았다. 즉, 자근 수침액의 투여는 B(a)P에 의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the pharmacologicalactivities of cn de Lithospermi Radix reported with the clinical uses in the orientalmedicine. Crude Lithospermi Radix uesd for the treatment of burn, eczema,blister, diuretic, scarlet fever and septicemia as antipyretic, antidotic and antiph-logistics. Therefore we tested the effects of Lithospermi Radix water extracton the liver-protective activities in the rats. The results obtained from enzymeassay, measurement of serum and liver alanine·aspartate aminotransferase(ALT, AST) and lipid composition indicated that Lithospermi Radix water extractshowed significant liver-protective activities against benzo(a)pyrene-induced he-patotoxicity.

천연자원을 이용한 간기능 증진제 개발 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 천궁 추출액의 영향 The Effect of Cnidii Rhizoma on Benzo(a) Pyrene Induced Hepatotoxicity

尹水弘,이송애,하헌,박은주,오관현 대구효성가톨릭대학교 1993 연구논문집 Vol.46 No.1

The present study was undertaken to compare the pharmacological activity of crude Cnidii Rhizoma reported with the clinical uses in the oriental medicine. Crude Cnidii Rhizoma used for the treatment of anemia, body chill, menstural irregularity and menstural pain as hematic tonic effector, sedative, adodyne and clear extravasated blood. Therefore we tested the effects of Cnidii Rhizoma water extract on the liverprotective activities in the rats. The results obtained form liver microsomal enzyme assay, measurement of serum and liver alanine, aspartate aminotransferase and lipid accumulation indicated that Cnidii Rhizoma extract showed significant liver-protective activities against benzo(a)pyrene poisoning.

천연자원을 이용한 간기능 증진제 개발 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 천궁 추출액의 영향 The Effect of cnidii Rhizoma on Benzo(a)pyrene Induced Hepatotoxicity

윤수홍,이송애,하헌,박은주,오관현 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

민간에서 주로 사용되는 약용식물들 중 간독성 의 발현 저해 및 간기능 보호 효과가 우수한약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험과정으로 탄소화합물의 불완전 연소및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의 간독성발현에 미치는 천궁 수침액의 예방 및 치료 효과를 실험하여 다음과 같은 결과를 얻었다. 1. 천궁 수침액의 투여는 B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT,LDH, ALP 활성을 유의성 있게 감소시켰다. 2. B(a)P 투여로 증가한 혈청 total cholesterol 및 phospholipid 함량은 천궁 수침액의투여로 감소시킬 수 있었으나 그 효과는 현저하지 않았다. 3. 천궁의 투여는 B(a)P에 의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the pharmacologicalactivity of crude Cnidii Rhizoma reported with the clinical uses in the orientalmedicine. Crude Cnidii Rhizoma used for the treatment of anemia, body chill,menstural irregularity and menstural pain as hematic, tonic effector, sedative,adodyne and clear extravasated blood. Therefore we tested the effects of Cnidii Rhizoma water extract on the liver-protective activities in the rats. The results obtained from liver microsomal enz-yme assay, measurement of serum and liver alanine, aspartate aminotransferaseand lipid accumulation indicated that Cnidii Rhizoma extract showed significantliver-protective activies against benzo(a)pyrene poisoning.

천연자원을 이용한 간기능 증진제 개발 연구 : Benzo(a) pyrene에 의해 유도된 간기능 장해에 미치는 세신 추출액의 영향 The Effect of Asiasari Radix on Benzo(a)pyrene Induced Hepatotoxicity

윤수홍,박은주,오관현 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

Effect of Asiasari Radix, which is a herbal drug used frequently in the oriental prescriptions, water extract on the liver-protective activities were investigated in the rats. Asiasari Radix extract, when administered into the gast-ric intubation, produced liver-protective effect against benzo(a)pyrene induced liver damage. The results obtainedfrom liver microsomal enzyme assay, measurement of serm and liver alanine·aspartate aminotransferase and lipid accumulation, indicated that alismatis extract showed significant liver-protective activities agai-nst benzo(a) pyrene poisoning.