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RNA Expression Profiles of Pepper (Capsicum annum) under StEREBP Gene by Using cDNA Microarray
Hwang, Eul-Won,Lee, Hye Eun,Byun, Myung-Ok,Kwon, Hawk-Bin 한국유전학회 2004 Genes & Genomics Vol.26 No.2
The StEREBP gene, which had previously been isolated from the potato plant, was introduced into potato plants under the control of the cauliflower mosaic virus (CaMV) 35S promoter for constitutive expression. The resulting transgenic plants showed visibly less cold damage compared with the empty vector transgenic control plants when both plants were stored in a -6℃ freezer for 24 hours. In addition, this study attempted to isolate the StEREBP regulated genes using a 3.1K cDNA chip spotted with the clones isolated from the cold-specific red pepper library. The microarray and Northern blot results revealed that 39 genes had a significantly differential expression level in the StEREBP transgenic plants. Of 39 genes, 34 and 5 genes were found to be up- and down-regulated, respectively. The genes for HSP90, a lichenase precursor, PR-1, a hexose transporter, dehydrin, peroxidase calreticulin, tyramine hydroxycinnamoyl transferase, the RD22 protein and the receptor protein kinase-related protein appeared to be up-regulated by StEREBP. However, the genes for the fruit ripening protein, the major-latex like protein and secretory peroxidase appeared to be down-regulated by StEREBP. In addition, the expression profiles of all 39 genes in response to cold, salt, ABA and drought stress were examined at the transcript level.
A Possible Role of Trehalose as a Regulatory Molecule in Plant Drought Resistance
Hwang, Eul-Won,Cho, Soo-Muk,Kwon, Hawk-Bin 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3
In many organisms, trehalose has been known as an energy source and a protectant against various environmental stresses such as desiccation, freezing, heat and osmotic pressure. Previously, we have isolated and characterized the genes encoding trehalose-6-phosphate synthase (ZrTPS1) and trehalose-6-phosphate phosphatase (ZrTPS2) from one of the most osmotolerant yeasts, Zygosaccharomyces rouazti We have also generated transgenic plants by co-introduction of ZrTPS1 and ZrTPS2 into potato plant (ZrTPS2-2AZrTPS1 plant) in an attempt to metabolically engineer trehalose in the transgenic plant using the foot-and-mouth disease virus (FMDV) 2A system and to generate drought resistant crop plants. In this research, we assayed previously generated the ZrTPS2-2A-ZrTPSI plant biofunctionally by drought treatment, and measured the amount of trehalose in the ZrTPS2-2A-ZrTPS1 transgenic plants. The ZrTPS2-2A-ZrTPS1 transgenic plant showed strong drought resistance in spite of little or no accumulation of trehalose in the transgenic plant compared with control plant.
miR171 Family Members are Involved in Drought Response in Solanum tuberosum
Hwang, Eul-Won,Shin, Seon-Ju,Yu, Byeong-Kuk,Byun, Myung-Ok,Kwon, Hawk-Bin 한국식물학회 2011 Journal of Plant Biology Vol.54 No.1
MicroRNAs (miRNAs) are a group of small noncoding RNAs found in both animals and plants. miRNAs function as negative post-transcriptional regulators during plant development and in response to biotic and abiotic stress. In this work, we identified drought stress-related miRNAs from reads obtained from second-generation sequencing. This method is useful to determine miRNA expression profiles when genomic sequences are not available and to find putative miRNAs by aligning reads with sequences from miRBase. Here, we report the identification of a family of drought-responsive miRNAs, stu-miR171a, stu-miR171b, and stu-miR171c, frompotato plants, their expression profiles upon drought stress, and their target mRNAs.
Hwang, Eu Chang,Yu, Seong Hyeon,Jo, Yang Hyun,Jung, Seung Il,Kang, Taek Won,Kwon, Dong Deuk,Choi, Chan,Heo, Suk Hee,Hwang, Jun Eul,Jung, Sung-Hoon,Jung, Tae-Young Medknow PublicationsMedia Pvt Ltd 2016 ASIAN JOURNAL OF ANDROLOGY Vol.18 No.1
<P>Several studies have revealed that the preoperative serum testosterone and percent tumor volume (PTV) predict extra-prostatic extension (EPE) and biochemical recurrence (BCR) after radical prostatectomy. This study investigated the prognostic significance of serum testosterone and PTV in relation to EPE and BCR after laparoscopic radical prostatectomy (LRP). We reviewed 520 patients who underwent LRP between 2004 and 2012. PTV was determined as the sum of all visually estimated tumor foci in every section. BCR was defined as two consecutive increases in the postoperative prostate-specific antigen (PSA) >0.2 ng ml<SUP>−1</SUP>. The threshold for serum total testosterone was 3.0 ng ml<SUP>−1</SUP>. Multivariate logistic regression was used to define the effect of variables on the risk of EPE and BCR. A low serum testosterone (<3.0 ng ml<SUP>−1</SUP>) was associated with a high serum PSA, Gleason score, positive core percentage of the prostate biopsy, PTV, and all pathological variables. On multivariate analysis, similar to previous studies, the serum PSA, biopsy positive core percentage, Gleason score, and pathological variables predicted EPE and BCR. In addition, low serum testosterone (<3.0 ng ml<SUP>−1</SUP>, adjusted OR, 8.52; 95% CI, 5.04–14.4, <I>P</I> = 0.001) predicted EPE and PTV (adjusted OR, 1.02; 95% CI, 1.01–1.05, <I>P</I> = 0.046) predicted BCR. In addition to previous predictors of EPE and BCR, low serum testosterone and PTV are valuable predictors of EPE and BCR after LRP.</P>
Overexpression of Zinc Finger Protein of Capsicum annuum(PIF1) in Tobacco Enhances Cold Tolerance
Eul Won Hwang,Soo Chul Park,Myung Ok Byun,Mie Young Choi,Hawk Bin Kwon 한국유전학회 2008 Genes & Genomics Vol.30 No.2
Plants encounter various stresses from their surrounding environment, such as low temperature, drought and high salinity. Thus the plant responds to abiotic stress such as cold and drought through a range of different strategies including physiological and biochemical processes that are usually mediated by up-regulation of the expression of stress regulated genes. Zinc finger proteins play a role in abiotic stress tolerance such as cold, dehydration and salt. As one way to approach to cold defense mechanism in plants, we previously identified a gene for zinc finger protein (PIF1) from hot pepper (Capsicum annuum) as cold inducible using cDNA microarray analysis coupled with Northern blot analysis. We previously showed that the PIF1 gene was induced strongly by cold stress, slightly by ABA. The PIF1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting PIF1 transgenic tobacco plants showed significantly increased cold stress resistance. In addition, all PIF1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results suggest the biological role of PIF1 in plant stress tolerances.
Eul-Won Hwang,신선주,박수철,Mi-Jeong Jeong,권혁빈 한국유전학회 2011 Genes & Genomics Vol.33 No.2
MicroRNAs (miRNAs) function as negative posttranscriptional regulators during plant development and in response to biotic and abiotic stress. In this study, we identified drought stress related miRNAs from sequencing reads obtained from second-generation sequencing. This method is useful for determination of miRNA expression profiles when genomic sequences are not available and for identification of putative miRNA by aligning reads with sequences from miRBase. Here, we present identification of a family of drought responsive miRNAs from potato plants, stu-miR172c,stu-miR172d, and stu-miR172e, and show expression profiles obtained in response to drought stress as well as their target mRNAs.
A Possible Role of Trehalose as a Regulatory Molecule in Plant Drought Resistance
Eul-Won Hwang,Soo-Muk Cho,Hawk-Bin Kwon 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3
In many organisms, trehalose has been known as an energy source and a protectant against various environmental stresses such as desiccation, freezing, heat and osmotic pressure. Previously, we have isolated and characterized the genes encoding trehalose-6-phosphate synthase (ZrTPS1) and trehalose-6-phos- phate phosphatase (ZrTPS2) from one of the most osmotolerant yeasts, Zygosaccharomyces rouxii. We have also generated transgenic plants by co-introduction of ZrTPS1 and ZrTPS2 into potato plant (ZrTPS2-2A- ZrTPS1 plant) in an attempt to metabolically engineer trehalose in the transgenic plant using the foot-and- mouth disease virus (FMDV) 2A system and to generate drought resistant crop plants. In this research, we assayed previously generated the ZrTPS2-2A-ZrTPS1 plant biofunctionally by drought treatment, and measu- red the amount of trehalose in the ZrTPS2-2A-ZrTPS1 transgenic plants. The ZrTPS2-2A-ZrTPS1 transgenic plant showed strong drought resistance in spite of little or no accumulation of trehalose in the transgenic pla- nt compared with control plant.
( Eul Won Hwang ),( Seon Ju Shin ),( Hawk Bin Kwon ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.3
MicroRNAs (miRNAs) are small noncoding RNAs that are found in both animals and plants. miRNAs function as negative post-transcriptional regulators during plant development and in response to biotic and abiotic stresses. In present study, high-throughput DNA sequencing and alignments with sequences from microRNA database (miRBase), a microRNA database, were used to identify drought stress-related miRNAs. This method is useful for identifying miRNAs and determining their expression profiles when genomic sequences are not available. Three droughtinduced miRNAs, stu-miR396, stu-miR156a, and stu-miR157a were identified in potato (Solanum tuberosum). Effects of drought stress on their expression profiles were demonstrated, and their target mRNAs were predicted.