http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ryoo, Kanghyun,Hwang, Sang-Gil,Kim, Kwang Je,Choi, Eui-Ju Dr. W. Junk B. V. Publishers ; Kluwer Academic Pub 2015 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.402 No.1
<P>RC3/neurogranin is a postsynaptic protein and plays pivotal roles in spatial learning and emotional anxiety as well as synaptic plasticity. The expression level of RC3 is dynamically changed during developmental stages, but the function of RC3 in brain development is not well understood yet. Neurotrophins interact with tropomyosin-related kinase receptors to activate Ras-extracellular signal-regulated kinase (ERK) pathway and can also induce neuronal differentiation. In this study, we demonstrate that RC3 inhibits Ras-ERK pathway by interaction with Ras and controls neurite outgrowth induced by neurotrophins. In PC12 cells, RC3 inhibits nerve growth factor (NGF)-induced activation of Ras and thereby ERK1/2 signaling cascade as well as neurite outgrowth induced by NGF. We found Ras is the target of the inhibitory function of RC3, because RC3 interacts with Ras and suppresses the elevated affinity of Ras to Ras-binding domain of Raf-1. Meanwhile, already activated Raf-1 by Ras activity is not affected by RC3. Furthermore, depletion of RC3 by RNA interference drastically enhances the stimulation of ERK1/2 and neurite outgrowth induced by brain-derived neurotrophic factor in hippocampal neurons. These findings suggest that RC3 is a novel natural inhibitor of Ras-ERK1/2 signaling axis, leading to negatively regulate neuronal differentiation induced by neurotrophins.</P>
유기초음파화학·초음파가 $BaMnO_4$ 및 $KMnO_4-CuSO_4{\cdot}5H_2O$를 이용한 알코올의 산화반응에 미치는 영향
유의상,신대현,한병희,Eui Sang Ryoo,Dae Hyun Shin,Byung Hee Han 대한화학회 1987 대한화학회지 Vol.31 No.4
초음파(50KHz)가 상온 상압하에서 $BaMnO_4$ 및 $KMnO_4-CuSO_4{\cdot}5H_2O$를 이용한 1차, 벤질, 2차 알코올의 알데히드 및 케톤 생성반응을 가속 완결시켰으며 고속교반이나 가열 환류반응보다 높은 산화율을 주었다. Sonic waves (50KHz) was accelerated the oxidation reaction of primary, benzyl and secondary alcohol with $BaMnO_4\;and\;KMnO_4-CuSO_4{\cdot}5H_2O$ to give the corresponding aldehyde and ketone at $30^{\circ}C/1$ atm. in high yields compared to stirring or refluxing condition.
Chung, Young-Soo,Jun, Tae-Won,Chung, Sung-Tai,Jung, Deog-Jo,Ryoo, Byung-Kwan,Eum, Woo-Sub,Shin, Eui-Shik 한국체육무용국제교류학회 2000 한국체육무용국제교류학회지 Vol.6 No.-
Recently, Jogging and distance running(including marathon) become popular physical activity in Korea, because these activity are beneficial to health maintainment and promotion. The presence of hills during road races, cross-country races, marathons is an importance consideration for joggers and distance runners. The physiological mechanism limiting performance on these course is not known. We examined the responses of uphills and horizontal with healthy young males(n=8, age = 27 yr) running on a treadmill. Treadmill protocol were: uphill course was Bruce protocol, and horizontal course was modified Bruce protocol(percent grade 10%, speed 1.7mph, and every three minute increased speed only). During running on treadmill, metabolic and blood lactate were measured. Metabolic variables(O2, CO2, VE, R) and blood lactate were higher in uphill running than horizontal running. A paired t-test indicated that CO2 and R were significant (p<0.01) difference between two running type. Results suggest that runners demonstrated different response to energy expenditure, blood lactate and muscle fatigue in uphill and horizontal running. Therefore, in order to run effectively, we try to apply training method minimize local fatigue restricting performance.
추수진 ( Soo Jin Choo ),류인자 ( In Ja Ryoo ),김영희 ( Young Hee Kim ),허광화 ( Guang Hua Xu ),김기호 ( Ki Ho Kim ),한창성 ( Chang Sung Han ),김수진 ( Su Jin Kim ),김진웅 ( Jin Woong Kim ),손의동 ( Eui Dong Son ),유익동 ( Ick Dong 대한화장품학회 2009 대한화장품학회지 Vol.35 No.2
천연물로부터 새로운 미백 소재를 개발하기 위하여 식물 추출물들의 미백활성을 조사한 결과, 엉겅퀴의 열매로부터 추출한 silymarin이 우수한 미백효과를 나타내는 것을 발견하였다. Silymarin으로부터 유효 성분을 분리하기 위하여 각종 컬럼 크로마토그래피 및 HPLC 등의 기법을 실시하여 silybin과 isosilybin을 분리한 후 이성질체인 silybin A와 B, 그리고 isosilybin A와 B를 각각 순수 분리하였다. Silymarin은 Mel-Ab melanocyte에서 세포독성에 영향을 주지 않는 동시에 멜라닌의 생성을 억제하였고 IC<sub>50</sub> 값은 28.2 μg/mL이었다. 또한 Silymarin은 cell-based tyrosinase의 활성을 저해하고, western blot 분석 결과 tyrosinase 단백질의 발현을 감소시키는 것을 확인하였다. Silymarin으로부터 분리한 활성 화합물인 silybin 및 isosilybin의 미백 효과를 측정한 결과, 각각 42.25 μM 및 16.32 μM의 IC<sub>50</sub> 값을 가지며 멜라닌의 생성을 억제하였으며 tyrosinase 단백질의 발현을 감소시켰다. Diastereoisomer 형태로 존재하는 silybin A 및 B, 그리고 isosilybin A 및 B의 멜라닌 저해활성을 측정한 결과, 네 가지 화합물 모두 농도 의존적으로 멜라닌의 생성을 억제하는 것으로 나타났다. 또한, 피부 미백 임상연구를 실시한 결과, silymarin 2 % 함유 크림을 사용할 경우 피부 미백 효과가 유효하게 나타남을 확인하였다. 이상의 결과로부터 본 활성물질 silymarin은 피부 미백 효과가 우수한 안전한 화장품 원료로서 사용할 수 있을 것으로 사료된다. We found that silymarin exhibited the inhibitory effect on melanogenesis in a spontaneously immortalized mouse melanocyte cell line, Mel-Ab. Silymarin is a standardized extract obtained from the dried seeds of milk thistle (Silybum marianum Gaertn.). Silymarin significantly prevented melanin production in a dose-dependent manner with an IC<sub>50</sub> value of 28.2 μg/mL without effects on cell viability. Also, silymarin inhibited tyrosinase activity in melanocyte, while it did not affect the catalytic activity of cell-free tyrosinase. Furthermore, Western blot analysis indicated that silymarin decreased the expression of tyrosinase protein. Silybin A/B and isosilybin A/B were also able to inhibit melanin production and tyrosinase expression in protein level. Double blind study on the clinical efficacy of a cream containing 2 % silymarin showed that silymarin have a significant skin whitening effect. Therefore, this study suggests that silymarin may be useful as a natural skin whitening agent.