돼지 내부기생충(內部寄生蟲)의 현황(現況)과 돼지 배분(排糞)의 검사성적(檢査成績)

장두환,Jang, Du Hwan 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.2

In survey for internal parasites of 395 heads of swine by fecal examination at ict, incidences of each parasite were obtained as follows: Giardia lamblia 1.0% Entamoehaspp. 55.4 Eimeria& Isospora spp. 22.5 Balantidium coli 66.6 Metastrongylus elongatus 17.6 Ascaris suum 25.6 Oesophagostomun dentatum 29.1 Hyostrongylus ryubidus 14.6 Trichuris suis 4.2 Strongyloides ransomi 7.2 Mecistocirrus digitatus 1.0 Check-list for the internal parasites of swine made by all the materials repor years from 1920 to 1975 in Korea is as follows: No. Parasites Habitat References 1. Ascaris lumbricoides small intestine Kawamura(1923) 2. Oesophagostomum dentatum large intestine Kawamura(1923) 3. Echinococcus veterinorum lung & liver Kawamura(1923) 4. Cysticercus cellulosae muscle Yunoba(1923) 5. Sarcooystis sp. muscle Arahayase(1927) 6. Entamoeba polecki intestine Kuwabara(1931) 7. Balantidium coli large intestine Huruyama(1931) 8. Metastrongylus elongatus lung Lee(1956) 9. Gongylonema pulckrum oesophagus Isshiki(1960) 10. Ascarops strongylina stomach Isshiki(1960) 11. Cysticercus tenuicollis peritoneum Isshiki(1960) 12. Cysticercus bovis? diaphragm Isshiki(1960) 13. Toxoplasma gondii interna organs Mun(1960) 14. Trichuris suis large intestine Lee et al.(1963) 15. Stephamirus dentatus feces Lee et al.(1963) 16. Spirometra mansonides fat layer of muscle Jang(1964) 17. Hyostrongylus rubidus stomach Kim et al.(1969) 18. Strongyloides ransomi feces Kim et al.(1969) 19. Eimeria perminuta feces Jang(1972) 20. E. debrieki feces Jang(1972) 21. E. polita feces Jang(1972) 22. E. scabra feces Jang(1972) 23. E. scrofae feces Jang(1972) 24. Isospora suis feces Jang(1972) 25. Entamoeba coli feces Jang(1975) 26. Mecistocirrus digitatus feces Jang(1975) 27. Giardia lamblia feces Jang(1975).

흡충류인 간질, 췌질 및 칼리코포론쌍구흡충의 염색체에 관한 연구

장두환,노재욱,강두원,김병기,김성원,Jang, Du-hwan,Noh, Jae-wuk,Kang, Du-weon,Kim, Byung-ki,Kim, Sung-won 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.3

Chromosomes of gonadal tissues from Fasciola spp, Eurytrema pancreaticum and Calicophoron calicophorum occurred Korean cattle were egamined using modified air-drying method. To compare their phenotype with three different genotypes among Fasciola spp, the adult and egg si2e were measured since they have been known as important taxonomical characters. The results obtained were as followed; Cattle liver fluke, Fasciola spp were classified into three types based on their chromosomal complements such as individual with 2o chromosome(diploid), 30 chromosome(triploid) and 20/30 mosaic constitution(mixoploid). The propotions of appearance of three types were 40.00%, 54.29% and 5.71%, respectively. The frequency of three types in type I which was regarded as F gigantica were 58.82% for diploid, 35.29% for triploid and 5.88% for mixoploid, but in type II which was regarded as F hepatica were 72.2% for triploid, 22.22% for diploid and 5.56% for mixoploid. Egg length of triploid forms was significantly larger than that of diploid forms and egg size of mixoploid forms was similiar to that of triploid forms. Worm size of triploid forms was larger than that of diploid forms and was more similar to that of mixoploid forms, but the statistical data were not significant. Diploid chromosome consisted of one pair of metacentric chromosome(m), four pairs of submetacentric chromosomes(sm), five pairs of subtelocentric chromosomes(st), while triploid chromosome consisted of one pair of metacentric chromosome, seven pairs of submetacen.tric chromosomes, one pair of subtelocentric chromosome and telocentric chromosome(t), respectively. In mixoploid chromosome, constitution of the chromosomes of diploid or triploid cell was consistent with that of diploid or triploid. Chromosomes of gonadal tissues from pancreatic fluke, Eurytrema pancreaticum consisted of 13 pairs of homologs(2n=26, n=13). The mitotic and meiotic divisions were observed frequently. In the mitotic metaphase, Karyotype consisted of five pairs of metacentric chromosomes, four pairs of submetacentric chromosomes, three pairs of subtelocentric chromosomes and one pair of telocentric chromosome. Chromosomes of gonadal tissues from stomach fluke, Calicvphoron calicophorum consisted of 9 pairs of homologs(2n=18, n=9). The meiotic divisions was frequently observed, but mitotic divisions was rare. In the mitotic metaphase, Karyotype consisted of two pairs of metacentric chromosomes, three pairs of submetacentric chromosomes and four pairs of subtelocentric chromosomes. Karyotype of Calicophoron calicophorum differed from that of Japanese C calicophorum which was similar to that of Paramphistomum cervi of Korean cattle. Though that of Calicophoron calicophorum of Korean cattle was similar to that of Paramphistomum explanatum of Korean cattle, that have been recognized to be a different species of fluke.

췌질(膵蛭)에 관한 연구(硏究) [III] 충란검사법(蟲卵檢査法)의 개량(改良)

장두환,Jang, Du Hwan 대한수의학회 1970 大韓獸醫學會誌 Vol.10 No.1

The techniques which have been used for the fecal examination of ruminant infected with the pancreatic flukes, Eurytrema Pancreaticum, were reviewer in their efficiency to detect the ova. One of modified fecal examination: H.F.E. (hydrochloric acid-formalin-ether) sedimentation method was devised in this study. Efficiency in the detecting ability of the fluke eggs with H.F.E. sedimentation method was determined by a series of repeat tests. Among 20 head of cattle known to harbor 1-5 adult worms of the pancreatic fluke, 75% of the infected cattle were detected, and among 60 head of cattle known to harbor more than 6 adult worms, 95% of the infected cattle were detected with H.F.E. sedimentation method. The procedures of the H.F.E. sedimentation method are as follows; 1) Take the sample 5-10 gm., emulsify throughly with 20 ml. of 50% hydrochloric acid in a cup. 2) Strain this mixture through one or two layers of wet surgical gauze into 15ml. centrifuge tube. 3) Washing the cup with 5ml. of 50% hydrochloric acid and strain again. 4) Centrifuge at 2,300 rpm. for 2 minutes. 5) Pour off the supernatant fluid. 6) After the sediment mixed with 10% formalin, stand for 5 minutes. 7) Add 2-3ml. of ether, shake vigorously up and down, after the top of the tube covered with thumb. 8) Centrifuge at 2,300 rpm. for 2 minutes. 9) Loosen the fecal plug in the tube by ringing with an applicator stick. 10) Quickly, but carefully, pour of all, but the bottom layer of sediment. 11) Thoroughly mix the sediment, pour on a slide (or pick up it with a pipett), mount with a cover glass. 12) Examine carefully.

췌질(膵蛭)에 관(關)한 연구(硏究) [I] 감염율(感染率)과 병변(病變)

장두환,Jang, Du Hwan 대한수의학회 1969 大韓獸醫學會誌 Vol.9 No.2

Researches to determine the infectious status on the pancreatic fluke, Eurytrema pancreaticum(Janson 1889) Looss, 1907, were made into several slaughter hauses at four different districts through seven years from 1963 to 1969. The infection rate showed 2962 positive of 5974 heads (49.6%) of cattle at the Seoul first slaughter hause in Seoul, 881 positive of 1021 heads (86.3%) in Cheju, and six(6) positive of 56 heads (11.1%) at Suwon, 58 positive of 306 heads(19.0%)at Joenju. The infection rate 53.1%, (2907 positive heads of total 7357 heads exammed) is the representative infection rate at the cattle eurytremiasis in Korea. It showed little variation through a year and was increased as compared with those of the past period, from 1915 to 1945. Fecal examinations in order to find out the local distribution of the pancreatic fluke were conducted on cattle, sheep, and goats raised in four districts, showing two(2) of 269 heads (0.7%) in Kyonggi-do, 41 of 212 heads (19.3%) in Cheju-do, and negative in both Chungnam-do and Joenbuk-do. When an artificial infection wss performed on rabbits and goats with metacercaria of the pancreatic fluke, Parenchyma of their pancreas were degenerated and the ducts were dilated. When cattle and sheep were infected naturally, the pancreatic ducts were also dilated, the wall was thickened, and adenomatous and epithelial hyperplasia was appeared. The conclusion, putting the foregoing pathological changes, has been arrived at chronic hyperplastic pancreatitis in the eurytremiasis of cattle, sheep, goats and rabbits.

소(우)의 흡충류 및 소화관내선충류에 대한 Levamisol HCl 및 Oxyclozanide 합제의 구충효능시험

장두환,지차호,윤희정,Jang Du-Hwan,Jee Cha-Ho,Youn Hi-Joeng 대한수의사회 1984 대한수의사회지 Vol.20 No.5

A total of 290 cattle in several districts was selected for treatment against the liver flukes, paramphistomes, and the gastrointestinal roundworms from March to september, 1983. 1. Egg reduction rates against the liver flukes, paramphistomes, and gastroi

분에 배설되는 기생충의 충난과 원충의 낭자를 검사하는데 사용하는 침전법과 부유법의 비교 (제1회)

장두환,JANG Du Hwan 대한수의사회 1961 대한수의사회지 Vol.5 No.2

임상수의사를 위한 소동물의 내부기생충에 대하여

장두환,Jang Du Hwan 대한수의사회 1980 대한수의사회지 Vol.16 No.3

간질피낭유충(肝蛭被囊幼蟲)의 생산(生産)에 관한 연구(硏究)

장두환,윤희정,전계식,Jang, Du-hwan,Youn, Hi-jeong,Jun, Ge-sik 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2

The cattle liver flukes were collected at daughter house and classified with gross morphology. The hepatica type was only 14 flukes (4.7%) and the gigantica type was 284 flukes (95.3%) among total 298 flukes collected. In order to determinate the intermediate host of the flukes, artificial infection with the miracidia to snail hosts, Radix auricularia and Austropelea ollula, were successfully carried out, but the development in the later was terminated at the sporocyst stage. Intermediate host of the cattle flukes used to hibernate in deep part of the stubbles of rice pady. The number of A. ollula that has been collected from a rice stubble was ranged 0 to 19. Their mean number was 7 snails per the stubble. In artificial infection with miracidia, the reasonable number of them were 1 to 9 and the snail size was 2 to 5mm in length. The productivility of metacercaria in the intermediate host was observed. The metacercarial production per snail was ranged 5.5 to 48.0 per one miracidium.

고산지(高山地)에 정착(定着)한 쇠가죽파리, Hypoderma sp.에 관한 연구(硏究)

장두환,한홍률,정재영,Jang, Du-hwan,Han, Hong-ryul,Chung, Jae-young 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2

The exotic ox-warble fly, Hypoderma bovis has been established its life cycle at the high mountain region on the middle part of Korea. Milk cattle and Korean cattle of S pasture, H pasture and farms were infested with the third stage larva of the flies. Infection rates were 54.5% in S pasture and 30.4% in H pasture or farms. The second or third stage larve were detected from the back side of cattle and the muscle around epidual fat at autopsy. The grubs of ox-warble fly were throughly identified as Hypoderma bovis by the characteristics of spines or tubercles on the segments and stiginal plates.

한우(韓牛)의 증식저해(增殖沮害) 요인(要因)에 관한 연구(硏究) -기생충학적(寄生蟲學的) 및 의화학적(醫化學的) 조사(調査)-

장두환,신재두,Jang, Du Hwan,Shin, Jae Doo 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.2

Parasitological and biochemical studies for 82 heads (12.2%) showing sterility syndrom out of 672 heads of Korean native cattle were undertaken by analysis of blood values for their contents and examination of their vaginal swabs for Tritrichomonas foetus 1. Tritrichomonas infection of Korean cattle revealed 7 positive cases (8.54%) out of 82 infertile heads. 2. Contents of their serum protean, carotenoid, vitamin A and phosphorus were determined in summer and winter season and the following results were obtained. a) In summer season, average blood value of 30 cattle showing sterility syndrom were determined as protein 7.52g/100ml, carotenoid 165.1g/100ml, vitamin A 173.9 IU/100ml and phosphorus 7.48mg/100ml, respectively. b) In winter. season, average blood value of 52 cattle showing sterility syndrom were determined as protein 7.76g/100ml, carotenoid 413.3g/100ml, vitamin A 174,3 IU/100ml and phosphorus 7.54mg/100ml, respectively.