DROSHA targets its own transcript to modulate alternative splicing

Lee, Dooyoung,Nam, Jin-Wu,Shin, Chanseok Cold Spring Harbor Laboratory Press 2017 RNA Vol.23 No.7

<P>The nuclear RNase Ill enzyme DROSHA interacts with its cofactor DGCR8 to form the Microprocessor complex, which initiates microRNA (miRNA) maturation by cleaving hairpin structures embedded in primary transcripts. Apart from its central role in the biogenesis of miRNAs, DROSHA is also known to recognize and cleave miRNA-like hairpins in a subset of transcripts without apparent small RNA production. Here, we report that the human DROSHA transcript is one such noncanonical target of DROSHA. Mammalian DROSHA genes have evolved a conserved hairpin structure spanning a specific exon-intron junction, which serves as a substrate for the Microprocessor in human cells but not in murine cells. We show that it is this hairpin element that decides whether the overlapping exon is alternatively or constitutively spliced. We further demonstrate that DROSHA promotes skipping of the overlapping exon in human cells independently of its cleavage function. Our findings add to the expanding list of noncanonical DROSHA functions.</P>

RNA interference-mediated simultaneous silencing of four genes using cross-shaped RNA

Lee, Tae Yeon,Chang, Chan Il,Lee, Dooyoung,Hong, Sun Woo,Shin, Chanseok,Li, Chiang J.,Kim, Soyoun,Haussecker, Dirk,Lee, Dong-ki Springer-Verlag 2013 Molecules and cells Vol.35 No.4

MicroRNA–target interactions: new insights from genome-wide approaches

Lee, Dooyoung,Shin, Chanseok Blackwell Publishing Inc 2012 Annals of the New York Academy of Sciences Vol.1271 No.1

<P>MicroRNAs (miRNAs) are key posttranscriptional regulators of gene expression involved in diverse biological pathways in bilateral animals and plants. The key to understanding the biological function of a miRNA is to identify its regulatory targets. Although a few miRNA targets have been identified genetically, the rapidly expanding list of miRNAs has necessitated genome-wide tools for identifying target mRNAs, and a number of computational and experimental approaches have consequently emerged. Some of these approaches have also provided insights into the mechanistic aspects of miRNA-mediated regulation, another intensely debated area in the miRNA field. Here, we review several emerging features of miRNA–target interactions in animals and genome-wide approaches for probing those interactions.</P>

Emerging roles of DROSHA beyond primary microRNA processing

Lee, Dooyoung,Shin, Chanseok Informa UK (TaylorFrancis) 2018 RNA BIOLOGY Vol.15 No.2

<P>DROSHA is the catalytic subunit of the Microprocessor complex, which initiates microRNA (miRNA) maturation in the nucleus by recognizing and cleaving hairpin precursors embedded in primary transcripts. However, accumulating evidence suggests that not all hairpin substrates of DROSHA are associated with the generation of functional small RNAs. By targeting those hairpins, DROSHA regulates diverse aspects of RNA metabolism across the transcriptome, serves as a line of defense against the expression of potentially deleterious elements, and permits cell fate determination and differentiation. DROSHA is also versatile in the way that it executes these noncanonical functions, occasionally depending on its RNA-binding activity rather than its catalytic activity. Herein, we discuss the functional and mechanistic diversity of DROSHA beyond the miRNA biogenesis pathway in light of recent findings.</P>

MMPI-2 군집 분석을 통한 과학기술원 대학상담센터 내담자의 유형 분류

이상일(Lee Sangil),이유경(Lee Yugyeung),정두영(Jung Dooyoung) 부경대학교 인문사회과학연구소 2021 인문사회과학연구 Vol.22 No.2

본 연구의 목적은 과학기술원 대학상담센터를 방문한 대학생의 유형을 분류하고 그 특성을 확인하는 것이다. 2019년 1월부터 12월까지 과학기술원 내 대학상담센터를 방문한 학생들의 미네소타 다면적 인성검사 제 2판(Minnesota Multiphasic Personality Inventory-2, 이하 MMPI-2) 자료를 사용하였다. 구체적으로 타당도 척도와 임상척도, 그리고 내용 척도와 보충 척도 자료를 사용해 대학상담센터를 방문한 학생들의 특성을 파악하고자 하였다. 최종 분석과정에서는 총 151명의 MMPI-2 타당도 및 임상 척도를 사용해 군집분석을 실시하였다. 그 결과 총 3개의 유형으로 분류되었다. 분류된 유형의 특징을 내용 척도와 보충 척도를 통해 구체화 하였다. 결과는 다음과 같다. 유형 1은 ‘경미한 주관적 불편감 유형(총 60명, 39.74%, 척도 2 = 64.13점, 척도 7 = 62.05점, 척도 0 = 63.40점, ANX = 65.03점, DEP = 62.78점, LSE = 61.15점, SOD = 64.37점, WRK = 62.90점, Mt = 65.80점)’. 유형 2는 ‘심한 주관적 고통 호소 유형(총 28명, 18.54%, FBS = 67.29점, 척도 7 = 75.04점, 척도 2 = 72.14점, 척도 8 = 66.36점, ANX = 76.36점, DEP = 71.43점, HEA = 62.36점, OBS = 61.61점, Mt = 73.25점, PK = 69.57점, A = 66.93점)’, 유형 3은 ‘정상 프로파일 유형(총 63명, 41.72%, 타당도 척도와 임상척도를 비롯해 모든 척도들이 정상범위에 속함)’으로 정의되었다. 특히, 유형 2인 ‘심한 주관적 고통 호소 유형’의 경우 정서적 고통감과 낮은 자존감 그리고 자살 위험성을 나타내는 척도들의 상승이 나타나 주의 깊은 관심이 필요할 것으로 여겨진다. 한편, 각 유형들에서 비방어적인 태도와 낮은 외적 공격성향이 공통적으로 나타났다. 일반 종합대학 재학생들을 대상으로 한 선행 연구들에서 방어적 집단이나 신경증적/정신증적 문제를 시사하는 프로파일 유형이 나타나지 않고 주관적 불편감 수준으로 유형이 분류되었다는 점은 주목할 만하다. 마지막으로, 본 연구 결과에 대한 치료적 함의점과 제한점을 논의하였다. The purpose of this study was to classify subtypes of university student who visited the student counseling center of institute of science and technology and to identify their characteristics. Data from the Minnesota Multiphasic Personality Inventory-2 of students who visited the university counseling center in the institute of science and technology from January to December 2019 were used. Specifically, we tried to understand the characteristics of students who visited the university counseling center using the validity scale, clinical scale, content scale and supplementary scale data. In the final analysis process, a total of 151 MMPI-2 validity and clinical scales were used to perform cluster analysis. As a result, it was classified into three types. The characteristics of the classified types were concretized through content and supplementary scales. Type 1 was defined as Types of mild subjective discomfort(60, 39.74%, Scale 2=64.13, 7=62.05, 0=63.40, ANX=65.03, DEP=62.78, LSE=61.15, SOD=64.37, WRK=62.90, Mt=65.80), Type 2 as Types of severe subjective pain appeals(28, 18.54%, FBS=67.29, Scale 7=75.04, 2=72.14점, 8=66.36점, ANX=76.36, DEP=71.43, HEA=62.36, OBS=61.61, Mt=73.25, PK=69.57, A=66.93) , and Type 3 as Normal profile type (63, 41.72%, all scale including the validity scale and clinical scale are within the normal range) . In particular, Type 2 as ‘types of severe subjective pain appeals’ is considered that careful attention is needed because the scales indicating severe emotional pain, low self-esteem, and suicidal risk were elevated. On the other hand, non-defensive attitude and low external aggression were common in each type. And it is noteworthy that unlike the results of prior studies with general university students, no profile types suggesting defensive attitude, neurotic or psychotic problems appear and that the types are classified to subjective levels of discomfort. Finally, the therapeutic implications and limitations of this study were discussed.

The Association between Excessive Internet Gaming Behavior and Immersive Tendency, Mediated by Attention Deficit/Hyperactivity Disorder Symptoms, in Korean Male University Students

Jung Dooyoung,Shim Eun-jung,Park Hyeonggyu,Lee Kwanglo,Lee Sangil,Kim Eun-young,Chang Jae Seung,정성훈,Kim Yeni,Ahn Yong Min,Hahm Bong-jin 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.5

Objective Problematic online gaming (POG) and problematic Internet use (PIU) have become a serious public mental health problem, with Internet gaming disorder (IGD) included in “Conditions for further study” section of DSM-5. Although higher immersive tendency is observed in people affected by POG, little is known about the simultaneous effect of immersive tendency and its highly comorbid mental disorder, attention deficit/hyperactivity disorder (ADHD). This study aimed to assess the relationship between immersive tendency, ADHD, and IGD. Methods Cross-sectional interview study was conducted in Seoul, Korea with 51 male undergraduate students; 23 active gamers and 28 controls. Results Current ADHD symptoms showed partial mediation effect on the path of immersive tendency on POG and PIU. The mediation model with inattention explained variance in both POG and PIU better than other current ADHD symptom models (R2=69.2 in POG; 69.3 in PIU). Childhood ADHD symptoms models demonstrated mediation effect on both POG and PIU which explained less variance than current ADHD symptom models (R2=53.7 in POG; 52.1 in PIU). Current ADHD symptoms, especially inattention, appear to mediate the effect of immersive tendency on POG/PIU. Conclusion Immersive tendencies may entail greater susceptibility to IGD, and comorbidity with ADHD may mediate the effect of immersive tendency on IGD.

Longitudinal Association of Poor Sleep Quality With Chemotherapy-Induced Nausea and Vomiting in Patients With Breast Cancer :

Jung, Dooyoung,Lee, Kwang-Min,Kim, Won-Hyoung,Lee, Joo-Young,Kim, Tae-Yong,Im, Seock-Ah,Lee, Kyung-Hun,Spiegel, David,Hahm, Bong-Jin Ovid Technologies (Wolters Kluwer) - Lippincott Wi 2016 Psychosomatic medicine Vol.78 No.8

<P>Objective Risk factors for chemotherapy-induced nausea and vomiting (CINV) include older age, female sex, alcohol consumption, and a history of motion sickness. Although gastrointestinal symptoms are found to be related with sleep and mood in other conditions, little is known about their effects on CINV. Methods This prospective observational study recruited patients with early-stage breast cancer who had recovered from surgery before receiving a first cycle of anthracycline and cyclophosphamide-based chemotherapy. Candidate factors associated with CINV were assessed before chemotherapy by using the following: the Pittsburgh Sleep Quality Index, the Insomnia Severity Index, the Epworth Sleepiness Scale, and the Hospital Anxiety and Depression Scale. Chemotherapy-induced nausea (CIN) and chemotherapy-induced vomiting (CIV) were defined according to a numeric rating scale (0-10) as follows: 3, nausea; 1, vomiting. Results Between February 2012 and May 2014, data were collected from 198 patients. Chemotherapy-induced nausea occurred in 35.4% of patients, and CIV occurred in 31.3%. Chemotherapy-induced nausea was significantly associated with poor sleep quality (odds ratio [OR], 2.48; 95% confidence interval [CI], 1.13-5.46; p = 0.024) and pretreatment nausea (OR, 4.81; 95% CI, 1.84-12.62; p = 0.001). Likewise, CIV was significantly associated with poor sleep quality (OR, 2.64; 95% CI, 1.21-5.78; p = 0.015) and pretreatment nausea (OR, 3.07; 95% CI, 1.23-7.66; p = 0.016). Conclusions Poor sleep quality increases risk of CINV in patients with breast cancer. Sleep problems should be assessed and considered in the management of CINV.</P>

워크플로우 기반 스마트 빌딩 서비스 구현

이재희 ( Lee Jaehee ),김두영 ( Kim Dooyoung ),송세헌 ( Song Seheon ),이상일 ( Lee Sangil ),박재현 ( Park Jaehyun ) 한국정보처리학회 2017 정보처리학회논문지. 컴퓨터 및 통신시스템 Vol.6 No.6

IoT 기술 발전으로 사물 제어와 센서 정보 영역이 확대되고 서비스 또한 다양해지면서 에너지 소비가 높은 빌딩에서의 에너지 절감 노력이 점차 증가하고 있으며 생활 안전, 보안, 편의에 이르기까지 서비스가 확대되고 있다. 하지만 기존 스마트 빌딩 서비스들은 IoT 다바이스에 종속적이고, 사용 중인 서비스 변경 또는 확장이 어렵다. 본 논문에서는 사용자의 요구에 따라 서비스를 변경하거나 확장할 수 있도록 워크플로우 기반의 스마트 빌딩 서비스를 구현하였다. With the development of IoT technology, the scope of object controls and sensor information has expanded and services have been diversified. Energy saving efforts in buildings with high energy consumption is increasing, and services are being extended over life safety, security and convenience. However, Existing smart building services are dependent on IoT devices and are difficult to change or expand. In this paper, we have implement of workflow based smart building services to modify or extend services according to user`s needs.

가스터빈 블레이드 개발품의 확률론적 진동 평가

이두영(Dooyoung Lee),조철환(Cheolhwan Cho),김연환(Yeonhwan Kim),배용채(Yongchae Bae),이성호(Sungho Lee) 한국소음진동공학회 2012 한국소음진동공학회 학술대회논문집 Vol.2012 No.10

블레이드 소재 고주기피로특성 분석을 위한 공진형 가진 시험

이두영(Dooyoung Lee),김범수(Beomsoo Kim),김연환(Yeonhwan Kim),조철환(Chealhwan Cho),구재량(Jaeryang Goo) 한국소음진동공학회 2014 한국소음진동공학회 학술대회논문집 Vol.2014 No.4