Flavonoids of Rosa roxburghii Tratt Act as Radioprotectors

Xu, Ping,Zhang, Wen-Bo,Cai, Xin-Hua,Lu, Dan-Dan,He, Xiao-Yang,Qiu, Pei-Yong,Wu, Jiao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: To study the radioprotective effects of flavonoids from Rosa roxburghii Tratt (FRT). Materials and Methods: The radioprotective effects of FRT were investigated by examining cell viability, 30-day survival of mice and the number of colony-forming units in spleen (CFU-S) after total-body 60Co irradiation. Results: The survival rates of irradiated cells gradually increased with increasing concentrations of FRT. The survival rate was the highest at 87% with a concentration of $30{\mu}g/mL$. Pretreatment with FRT was needed to realize its radioprotective activity in mice at the dose of 60 mg/kg. With the increasing doses of 30 mg/kg, 60 mg/kg and 120 mg/kg, the numbers of CFU-S increased, and were significantly different compared with the control group. Conclusions: Pretreatment with FRT prior to irradiation resulted in significantly higher cell survival at 24 h after 5 Gy radiation, increased 30-day survival in mice after exposure to a potentially lethal dose of 8 Gy, and resulted in a higher number of CFU-S in mice after exposure to a dose of 6 Gy. These results collectively indicate that FRT is an effective radioprotective agent.

Circular Holes Punched in a Magnetic Circuit used in Microspeakers to Reduce Flux Leakage

Dan-Ping Xu,Yuan-Wu Jiang,Han-Wen Lu,Joong-Hak Kwon,Sang-Moon Hwang 한국자기학회 2016 Journal of Magnetics Vol.21 No.3

Lower flux leakage designs have become important in the development of microspeakers used in thin and miniaturized mobile phones. We propose four methods to reduce the flux leakage of the magnetic circuit in a microspeaker. Optimization was performed based on the proposed approach by using the response surface method. Electromagnetic analyses were conducted using the finite element method. Experimental results are in good agreement with the simulated results obtained in one degree-of-freedom analysis from 100 to 5 ㎑. Both the simulated and experimental results confirm that one of the proposed methods is much more effective in reducing flux leakage than the other methods. In the optimized method, compared with a default approach, the average radial flux density in the air gap decreased only by 5.5%, the maximum flux leakage was reduced by 28.6%, and the acoustic performance at primary resonance decreased by 0.45 ㏈, which gap is indiscernible to the human ear.

Association Between the Ku70-1310C/G Promoter Polymorphism and Cancer Risk: a Meta-analysis

Xu, Lu,Ju, Xiao-Bing,Li, Pu,Wang, Jue,Shi, Zhu-Mei,Zheng, Ming-Jie,Xue, Dan-Dan,Xu, Yan-Jie,Yin, Yong-Mei,Wang, Shui,You, Yong-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Ku70 plays an important role in DNA double-strand break repair. Studies revealing conflicting results on the role of the Ku70-1310C/G promoter polymorphism on cancer risk led us to perform a meta-analysis to investigate this relationship. Ten case-control studies with 2566 cases and 3058 controls were identified. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of associations. The overall results suggested no association between the Ku70-1310C/G promoter polymorphism and total cancer risk. However, on stratified analysis, significantly increased risks were observed among the Asian population (GG vs. CC: OR=1.50, 95%CI=1.10-2.06; GG vs. CC/CG: OR=1.47, 95%CI=1.07-2.01) and population-based case-control studies (GG vs. CC: OR=1.57, 95%CI=1.12-2.22; CG vs. CC: OR=1.35, 95%CI=1.11-1.64; CG/GG vs. CC: OR=1.37, 95%CI=1.14-1.65). Additionally, variant genotypes were associated with a significantly increased breast cancer risk (GG vs. CC: OR=1.80, 95%CI=1.26-2.56; GG vs. CC/CG: OR=1.40, 95%CI=1.01-1.95).

Fast Hybrid Transform

Dan-Ping Xu(쉬단핑),Dae-Chol Shin(신태철),Wei Duan(단위),Moon Ho Lee(이문호) 한국방송·미디어공학회 2011 방송공학회논문지 Vol.16 No.5

Amentoflavone Acts as a Radioprotector for Irradiated v79 Cells by Regulating Reactive Oxygen Species (ROS), Cell Cycle and Mitochondrial Mass

Xu, Ping,Jiang, En-Jin,Wen, Si-Yuan,Lu, Dan-Dan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Radioprotective effects of amentoflavone were investigated by examining cell viability, apoptosis, cell cycling concentrations of intracellular ROS (reactive oxygen species), and relative mitochondrial mass by flow cytometry after $^{60}Co$ irradiation. Pretreatment with amentoflavone 24 hours prior to 8 Gy $^{60}Co$ ${\gamma}$-ray irradiation significantly inhibited apoptosis, promoted the G2 phase, decreased the concentration of ROS and mitochondrial mass. These results collectively indicate that amentoflavone is an effective radioprotective agent.

Analysis of the Magnetization Effect of Permanent Magnets on the Nonlinear Magnetic Characteristic Distributions of a Balanced Armature Receiver

Xu, Dan-Ping,Jiang, Yuan-Wu,Hwang, Sang-Moon IEEE 2019 IEEE transactions on magnetics Vol.55 No.2

<P>Balanced armature (BA) receivers are widely used due to their small size and good performance. The magnetization of the permanent magnets (PMs) affects the nonlinear magnetic characteristics, which can affect the performance of a BA receiver. In this paper, four different magnetizations were simulated to examine the influence of magnetization on the cogging force, force factor, inductance, and speedance. The simulation results show that the nonlinearity is more obvious with larger magnetization of the PMs and approach a limit when there is saturation in the magnetic circuit. In addition, within a small vibration position or current range, the influence of the magnetization on the cogging force, force factor, and speedance can be treated as linear, and there is no influence on inductance. Based on the simulated nonlinear magnetic characteristics, the vibration displacement can be solved with coupling analyses. The cogging force also affects the displacement resonance frequency. The force factor has a big effect on the vibration displacement at low frequency. Two samples with different magnetizations were made, and the simulation results were verified experimentally.</P>

ZNF488 Enhances the Invasion and Tumorigenesis in Nasopharyngeal Carcinoma Via the Wnt Signaling Pathway Involving Epithelial Mesenchymal Transition

Dan Zong,Li Yin,Qian Zhong,Wen-jie Guo,Jian-hua Xu,Ning Jiang,Zhi-rui Lin,Man-zhi Li,Ping Han,Lin Xu,Xia He,Mu-sheng Zeng 대한암학회 2016 Cancer Research and Treatment Vol.48 No.1

Purpose The purpose of this study was to investigate the function of Zinc finger protein 488 (ZNF488) in nasopharyngeal carcinoma (NPC). Materials and Methods The endogenous expression of ZNF488 in NPC tissues, normal nasopharyngeal epithelium tissues and NPC cell lines were detected by quantitative reverse transcription polymerase chain reaction. ZNF488 over-expressing and knock-down NPC cell line models were estab- lished through retroviral vector pMSCV mediated over-expression and small interfering RNA (siRNA) mediated knock-down. The invasion and migration capacities were evaluated by wound healing and transwell invasion assays in ZNF488 over-expressing and control cell lines. Soft-agar colony formation and a xenograft experiment were performed to study tumorigenic ability in vitro and in vivo. Immunofluorescence and western blotting analysis were used to examine protein changes followed by ZNF488 over-expression. Microarray analysis was performed to explore gene expression profilings, while luciferase reporter assay to evaluate the transcriptive activity of Tcf/Lef. Results ZNF488 was over-expressed in NPC tissues compared with normal tissues, especially higher in 5-8F and S18, which are well-established high metastatic NPC clones. Functional studies indicate that over-expression of ZNF488 provokes invasion, whereas knock-down of ZNF488 alleviates invasive capability. Moreover, over-expression of ZNF488 promotes NPC tumor growth both in vitro and in vivo. Our data further show that over-expression of ZNF488 induces epithelial mesenchymal transition (EMT) by activating the WNT/β -catenin signaling pathway. Conclusion Our data strongly suggest that ZNF488 acts as an oncogene, promoting invasion and tumorigenesis by activating the Wnt/β -catenin pathway to induce EMT in NPC.

Supplement of High Protein-Enriched Diet Modulates the Diversity of Gut Microbiota in WT or PD-1H-Depleted Mice

( Yajun Xie ),( Ping Zhao ),( Zhigang Han ),( Wei Li ),( Dan Shi ),( Lei Xu ),( Qiying Yi ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.2

Supplement of high-protein food plays an important role in improving the symptoms of malnutrition and the immune capacity of the body, but the association of high-protein diet and gut microbiota remained unaddressed. Here, we systematically analyzed the internal organs and gut microbiota in C57(WT) or PD-1H-depleted (KO) mice (T cells were activated) fed with pupae or feed for six weeks. We observed that the body weight gain in the mice fed with pupae increased less significantly than that of the feed group, while the villi and small intestine lengths in the pupa group were reduced compared with that of mice given feed. However, the average body weight of the KO mice increased compared with that of the WT mice fed with pupae or feed. Pupae increased the concentration of blood glucose in WT, but not in KO mice. Moreover, in the feed group, there was no difference in the weight of the internal organs between the WT and KO mice, but in the pupae-fed group, liver weight was decreased and spleen weight was increased compared with that of KO mice. The amounts/plural/amounts of Melainabacteria, Chloroflexi, and Armatimonadetes were specifically upregulated by pupae, and this upregulation was weakened or eliminated by PD-1H depletion. Some bacteria with high abundance in the feed-fed KO mice, such as Deferribacteres, Melainabacteria, Acidobacteria, Bacteroidetes, Spirochaetes and Verrucomicrobia, were decreased in pupae-fed KO mice, and Proteobacteria and Deinococcus were specifically enriched in pupae-fed KO mice. Bacteroidetes, Firmicutes and Akkermansia were associated with weight loss in the pupaefed group while Lachnospiraceae and Anaerobiospirillum were related glucose metabolism and energy consumption. Based on high-throughput sequencing, we discovered that some gut bacteria specifically regulated the metabolism of a high-protein diet, and PD-1H deficiency improved life quality and sustained blood glucose. Moreover, PD-1H responses to high-protein diet through modulating the type and quantity of gut bacteria. These findings provide evidence about the association among gut microbiota, T cell activation (for PD-1H depletion) and high-protein diet metabolism, have important theoretical significance for nutrition and health research.

Clustering Strategy Based on Graph Method and Power Control for Frequency Resource Management in Femtocell and Macrocell Overlaid System

Li, Hongjia,Xu, Xiaodong,Hu, Dan,Tao, Xiaofeng,Zhang, Ping,Ci, Song,Tang, Hui The Korea Institute of Information and Commucation 2011 Journal of communications and networks Vol.13 No.6

In order to control interference and improve spectrum efficiency in the femtocell and macrocell overlaid system (FMOS), we propose a joint frequency bandwidth dynamic division, clustering and power control algorithm (JFCPA) for orthogonal-frequency-division-multiple access-based downlink FMOS. The overall system bandwidth is divided into three bands, and the macro-cellular coverage is divided into two areas according to the intensity of the interference from the macro base station to the femtocells, which are dynamically determined by using the JFCPA. A cluster is taken as the unit for frequency reuse among femtocells. We map the problem of clustering to the MAX k-CUT problem with the aim of eliminating the inter-femtocell collision interference, which is solved by a graph-based heuristic algorithm. Frequency bandwidth sharing or splitting between the femtocell tier and the macrocell tier is determined by a step-migration-algorithm-based power control. Simulations conducted to demonstrate the effectiveness of our proposed algorithm showed the frequency-reuse probability of the FMOS reuse band above 97.6% and at least 70% of the frequency bandwidth available for the macrocell tier, which means that the co-tier and the cross-tier interference were effectively controlled. Thus, high spectrum efficiency was achieved. The simulation results also clarified that the planning of frequency resource allocation in FMOS should take into account both the spatial density of femtocells and the interference suffered by them. Statistical results from our simulations also provide guidelines for actual FMOS planning.

Design of microspeaker module considering added stiffness

Peng Sun,Dan-Ping Xu,황상문 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.5

Mobile devices are becoming highly multi-functional due to the development of communication technology. The microspeaker moduleused in those devices, such as mobile phones or pads, is also playing important roles inside mobile devices. The conventional microspeakermodules normally have two demerits including a non-standardized total shape for different types of devices and a size toolarge for miniaturization design. This paper reports a novel structure design of a microspeaker module considering the added stiffness tominimize the total dimensions without affecting the acoustic performance. At the same time, a standardized novel microspeaker designwas applied to different types of mobile devices with sustained performance. The finite element method (FEM) was applied for electromagneticanalysis and dynamic analysis. The respond surface method (RSM) was used for optimization. As a design result, the totalvolume was decreased by 51.8%. The simulation showed that compared to the conventional design, the novel design maintains the resonancefrequency and sound pressure level (SPL) value. Prototypes were constructed and acoustic tests were conducted. The simulationresults were verified experimentally. The results revealed the novel design to have a similar resonance frequency with conventional design. In addition, the mean SPL value from 800 Hz to 5 kHz for the conventional and novel designs were maintained with 94.33 dB and94.98 dB, respectively, that means novel design has maintained acoustic performance as conventional design.