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The Mechanism of Antiangiogenic Effects of Korean Propolis in Human Umbilical Vein Endothelial Cells
Da-Hye Jeong,Mok-Ryeon Ahn 한국양봉학회 2023 韓國養蜂學會誌 Vol.38 No.4
We recently reported that the ethanol extracts of Korean propolis (EEKP) possesses anti- angiogenic activity both in vitro and in vivo. However, the mechanism of angiogenesis inhibition by Korean propolis have not been fully elucidated. In this study, we investigated the antiangio- genic effects of EEKP on tube-forming human umbilical vein endothelial cells (HUVECs). We found that inhibition of tube formation by EEKP was accompanied by partial fragmentation of endothelial cells, indicating that it induced cell death. Western blotting revealed that EEKP induced inactivation of Raf/MEK/ERK pathway in a concentration-dependent manner. EEKP also induced up regulation of p38 MAPK. It was shown that EEKP induced apoptosis via activation of pro-apoptotic signaling such as caspase cascades. Furthermore, EEKP induced the reduction of vascular endothelial cadherin(VE-cadherin) and platelet endothelial cell adhesion molecule-1 (PECAM-1). These results indicate that EEKP exerts its antiangiogenic effects through induction of HUVECs apoptosis. Korean propolis may have the potential to be developed into pharmaceutical drugs for the treatment of angiogenesis-dependent human diseases such as cancer.