Pyrophosphate-triggered nanoaggregates with aggregation-induced emission

Li, Chun-Tao,Xu, You-Liang,Yang, Jian-Gong,Chen, Yong,Kim, Hyeong Seok,Cao, Qian-Yong,Kim, Jong Seung Elsevier Sequoia 2017 Sensors and actuators. B Chemical Vol.251 No.-

<P><B>Abstract</B></P> <P>A novel tetraphenylethene-based probe bearing bis-imidazolium anion donors is herein reported for pyrophosphate anion recognition. This probe can self-assemble finite, small sphere nanoaggregates with very weak emission in aqueous solution, and changes into large rod-like nanoaggregates with strong aggregation-induced emission upon binding with the pyrophosphate anion.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A bis-imidazolium functionalized tetraphenylethene probe was prepared. </LI> <LI> This probe self-assemble finite small sphere nanoaggregates in aqueous solution. </LI> <LI> The probe can recognize pyrophosphate anion with strong aggregation-induced emission. </LI> <LI> The probe/pyrophosphate assembly can fluorescence assay alkaline phosphatase. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A novel nanoaggregates for recognition of pyrophosphate anion with aggregation-induced emission in pure aqueous solution is introduced.</P> <P>[DISPLAY OMISSION]</P>

Superhydrophobic ceramic hollow fiber membrane planted by ZnO nanorod-array for high-salinity water desalination

Wang, Tao,Yun, Yanbin,Wang, Manxiang,Li, Chunli,Liu, Guicheng,Yang, Woochul Elsevier 2019 JOURNAL- TAIWAN INSTITUTE OF CHEMICAL ENGINEERS Vol.105 No.-

<P><B>Abstract</B></P> <P>To solve the problems of membrane material tolerance and membrane fouling, a micro/nano hierarchial structure with low surface energy was constructed above the exterior surface of a ceramic hollow fiber membrane using ZnO nanorod arrays and 1H,1H,2H,2H-perfluorodecyltriethoxysilane (PDTS) coatings, which make it superhydrophobic and self-cleaning. The surface morphology, chemical functional groups, and water contact angel of the modified membranes were identified. The results show that large quantity of ZnO nanorods possess desirable characteristics (<I>i.e.</I> superhydrophobicity, exceptional thermal and mechanical stability, and water contact angle of 160.12°) were detected on the ceramic membrane. The novel membrane shows excellent self-cleaning performance and good desalination ability in the utilization of vacuum membrane distillation (VMD) system for high-salinity water desalination.</P> <P><B>Highlights</B></P> <P> <UL> <LI> • Ceramic membrane is modified by imitating of superhydrophobic biological surfaces. </LI> <LI> • The micro/nano hierarchical structure with low surface energy endows the superhydrophobicity. </LI> <LI> • The P-ZN-CHF membrane obtains antifouling ability. </LI> <LI> • The modified membrane shows excellent performances during vacuum membrane distillation. </LI> </UL> </P>

Systematic Review of Single Large and/or Multinodular Hepatocellular Carcinoma: Surgical Resection Improves Survival

Yang, Xiang-Di,Pan, Ling-Hui,Wang, Lin,Ke, Yang,Cao, Ji,Yang, Chun,Zhong, Jian-Hong,Luo, Wang,Guo, Jiao,Li, Le-Qun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13

Background: The role of surgical resection for patients with single large (${\geq}5cm$) and/or multinodular (${\geq}2$) hepatocellular carcinoma (HCC) is still controversial. This systematic review was performed to evaluate the safety and efficacy of resection for patients with single large and/or multinodular HCC. Materials and Methods: Databases (the PubMed, Web of Science, Embase, and Cochrane databases) were systematically searched to identify relevant studies exploring the safety and efficacy of resection for single large and/or multinodular HCC, published between January 2000 and December 2014. Perioperative morbidity and mortality, overall survival, and disease-free survival of the resection group were calculated. In addition, these outcome variables were also calculated for the control group in the included studies. Results: One randomized controlled trial and 42 nonrandomized studies involving 9,580 patients were eligible for analysis. Eight (1,594 patients) of the 43 studies also reported the outcomes of transarterial chemoembolization (TACE). Although 51.4% of patients featured cirrhosis, 90.7% of them demonstrated Child-Pugh A liver function in the resection group. The median rates of morbidity (24.5%) and mortality (2.5%) after resection were significantly higher than that of TACE (11.0%, P<0.001; 1.9%, P<0.001). However, patients who underwent resection had significantly higher median one-, three-, and five-year overall survival (76.1%, 51.7%, and 37.4%) than those who underwent TACE (68.3%, 31.5%, and 17.5%, all P<0.001). The median 1-, 3-, and 5-year DFS rates after resection were 58.3%, 34.6%, and 24.0%, respectively. Conclusions: Although tumor recurrence after resection for patients with single large and/ or multinodular HCC continues to be a major problem, resection should be considered as a strategy to achieve long-term survival.

Isolation of New Strain of Cordyceps militaris HB8 and Optimal Condition for Production of Adenosine and Cordycepin in Fruit Body

Li, Jin Feng,Hoang, Van An,Ahn, Jong Chan,Yang, Dong Uk,Lee, Dong Wook,Yang, Deok Chun The Plant Resources Society of Korea 2020 한국자원식물학회지 Vol.33 No.6

Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the new Cordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militaris JLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8 was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession number MT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 mg/g (200 g of pupa, 1 g of KH₂PO₄, 0.5 g of K₂HPO₄, 20 g of glucose, 1 g of MgSO₄, 0.05 g of vitamin B1, and 1 mg of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production of adenosine was 2.6 mg/g (15 g of skim milk powder, 1 g of KH₂PO₄, 0.5 g of K₂HPO₄, 20 g of glucose, 1 g of MgSO₄, 0.05 g of vitamin B1, and 1 mg of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

Identification of MicroRNAs as potential biomarkers for detecting ischemic stroke

Li Kexin,Shen Li,Zheng Pingping,Wang Yanjun,Wang Lijuan,Meng Xiaoli,Lv Yaogai,Xue Zhiqiang,Guo Xin,Zhang Anning,Pan Pan,Bi Chunli,Chen Yang,Feng Tianyu,Li Bo,Jin Lina,Yao Yan 한국유전학회 2022 Genes & Genomics Vol.44 No.1

Background: Increasing epidemic of ischemic stroke (IS) makes it urgent to understand the pathogenesis and regulatory mechanism, previous studies have described microRNAs (miRNAs) is part of the brain's response to ischemia. Objective: The aim of this study was to screen potential biomarkers for the prediction and novel treatment of IS. Methods: Differentially expressed miRNAs were screened from three newly diagnosed IS patients and three controls by RNA sequencing technology. Furthermore, target prediction databases were then used to analysis the target genes of different expressed miRNAs, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database were used to identify the functions and the main biochemical and signal pathways of differentially expressed target genes. Results: Our results revealed that 27 miRNAs were differentially expressed in IS, among which, hsa-miR-659-5p was the most highly increased and was first found to be associated with IS. In addition, KEGG pathway analyses showed that differentially expressed miRNAs were mainly significantly enriched in lysosome pathway, cytokine-cytokine receptor interaction pathway, spliceosome pathway, base excision repair pathway. Conclusions: miRNAs were involved in IS pathogenesis, and hsa-miR-659-5p, hsa-miR-151a-3p and hsa-miR-29c-5p as the three highest |log2FoldChange| regulation in this study, which may be the biomarkers of IS and need further study.

A multi-objective optimization for HAWT blades design by considering structural strength

Yang Yang,Chun Li,Wanfu Zhang,Jun Yang,Zhou Ye,Weipao Miao,Kehua Ye 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.8

The challenge of wind turbine blade design is to balance the conflict between high capacity and heavy system loads introduced by the large scale rotor. To solve this problem, we present a multi-objective optimization method to maximize the Annual energy production (AEP) and minimize the blade mass. The well-known Blade element momentum (BEM) theory is employed to predict the aerodynamic performance and AEP of the blade. The blade is simplified as a thin Bernoulli beam. The cross section is modelled as a combination of composite layer, shear webs and spar caps typically. The strain of every cross section has been considered as a constraint to minimize the spar cap thickness for minimizing the blade mass. An improved genetic algorithm (NSGA-II) is applied to obtain the Pareto front set. Several solutions of the Pareto set are selected to compare with the reference blade (NREL 5MW blade). Performance of the rotors on design condition is simulated by STAR-CCM+ to verify the results of BEM theory. Optimal results show that the present blade, which is fully superior to the reference blade, can be selected from the Pareto set. The optimization design method can provide a superior blade with an increase by 2.48% of AEP and a reduction by 5.52% of the blade mass. It indicates the present optimization method is effective. Results of numerical simulations show that the spanwise flow would be increased obviously in tip region of the reference blade. The reason is that chord length variation in blade tip affects the flow and causes minor stall. The abrupt change of chord distribution in blade tip should be avoided to reduce the spanwise flow in initial blade design.

Red Deer Antler Extract Accelerates Hair Growth by Stimulating Expression of Insulin-like Growth Factor I in Full-thickness Wound Healing Rat Model

Yang, Zhi Hong,Gu, Li Juan,Zhang, Dong Liang,Li, Zheng,Li, Jing Jie,Lee, Mi-Ra,Wang, Chun Yan,Wang, Zhen,Cho, Jeong-Hee,Sung, Chang-Keun Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.5

In order to investigate and evaluate the effects of red deer antlers on hair growth in the full-thickness wound healing model, Sprague-Dawley rats were given incision wounds through the full thickness of their dorsal skin and deer antler was applied for 40 days. At specified intervals thereafter (4, 8, 16, 32 and 40 days), the animals were sacrificed and the wound site skins were excised, processed, and sectioned. At post-injury days 16, 32 and 40, longer and more active new hair appeared around the healing wound of antler-treated skin. Histological studies showed that the antler extract markedly increases the depth, size, and number of hair follicles. Expression of IGF-I (insulin-like growth factor) mRNA was detected by RT-PCR and real time RT-PCR. The result showed that the expression of IGF-I (days 16, 32, and 40) was obviously up-regulated in antler-treated skins compared to control skins. Similar results were seen in the ELISA analysis to quantify the IGF-I expression. These results support the notion that wound healing can cause hair growth by enhancing the expression of IGF-I. Deer antler extract appears to have the potential to promote hair growth and could be used in hair growth products.

Genome-wide identification and evolutionary analysis of WOX gene family in cucurbit crops

Li Chun,He Zhen,Liang Genyun,Yang Nan,Cai Peng,Liang Ying,Li Yuejian,Fang Chao,Liu Duchen,Xia Feng,Liu Xiaojun 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1

The WUSCHEL-related homeobox (WOX) gene family is essential for plant growth and development. However, the identifi cation and evolutionary analysis of WOX genes in the Cucurbitaceae have not been well investigated. In the present work, we performed a genome-wide analysis to identify WOX genes in eleven cucurbit crops. In total, 127 WOX genes were identifi ed in eleven cucurbit crops, and classifi ed into three diff erent clades: the ancient clade, the intermediate clade and the WUS clade. The same clade has comparable gene organization and conserved motif patterns. The cis -acting ele ment analysis revealed that WOX genes were involved in light, hormone and stress in cucurbit crops. Gene duplication analysis indicated that dispersed duplication and whole genome duplication were the two primary causes of WOX genes expansion. There were 24 and 129 gene pairs found within cucurbit crops, and between Cucumis sativus and other cucur bit crops, respectively. After gene duplication, all WOX genes were subjected to substantial purifying selection pressure according to the selective pressure analysis. Functional divergence analysis revealed that ancient and intermediate clades may have descended from some essential amino acid sites rather than diff erent evolution rate. Our research provides a comprehensive analysis of WOX genes which will be helpful for further research into their function study in Cucurbita ceae plants.

Investigation on aerodynamics and active flow control of a vertical axis wind turbine with flapped airfoil

Yang Yang,Chun Li,Wanfu Zhang,Xueyan Guo,Quanyong Yuan 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.4

A 2D unsteady numerical simulation with dynamic and sliding meshing techniques was conducted to solve the flow around a threeblade Vertical axis wind turbine (VAWT). The circular wakes, strip-like wakes and the shedding vortex structures interact with each other result in an extremely unstable performance. An airfoil with a trailing edge flap, based on the NACA0012 airfoil, has been designed for VAWT to improve flow field around the turbine. Strategy of flap control is applied to regulate the flap angle. The results show that the flapped airfoil has an positive effect on damping trailing edge wake separation, deferring dynamic stall and reducing the oscillating amplitude. The circular wake vortices change into strip vortices during the pitch-up interval of the airfoils. Examination of the flow details around the rotating airfoil indicates that flap control improves the dynamic stall by diminishing the trend of flow separation. Airfoil stall separation has been suppressed since the range of nominal angle of attack is narrowed down by an oscillating flap. Vortices with large intensity over rotational region are reduced by 90 %. The lift coefficient hysteresis loop of flapped airfoil acts as an O type, which represents a more stable unsteady performance. With flap control, the peak of power coefficient has increased by 10 % relative to the full blade VAWT. Obviously, the proposed flapped airfoil design combined with the active flow control significantly has shown the potential to eliminate dynamic stall and improve the aerodynamic performance and operation stability of VAWT.

Isolation of Chemical Constituents from the Aerial Parts of Verbascum thapsus and Their Antiangiogenic and Antiproliferative Activities

Yan-Li Zhao,Yong-Ping Yang,Si-Feng Wang,Yang Li,Qiu-Xia He,Ke-Chun Liu,Xiao-Li Li 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.5

Phytochemical investigation of Verbascum thapsus led to the isolation and identification of one new iridoid compound named verbathasin A, along with ten known compounds. The structure and relative stereochemistry of verbathasin A were elucidated by analysis of spectroscopic data. All the isolates except 10-deoxyeucommiol and ajugol were tested for antiangiogenic and antiproliferative activities, and compounds luteolin and 3-O-fucopyranosylsaikogenin F showed promising antiproliferative activities, with an obvious effect of inducing apoptosis of A549 lung cancer cells.