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Yangdong Sun,Qiao Ye,Min Wu,Yonghong Wu,Chenggang Zhang,Weiqun Yan 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-
This study aimed to validate the high yield and soluble expression of proteins carrying the transactivator of transcription (Tat) peptide tag, and further explored the potential mechanism by which the Tat tag increases expression. Escherichia coli superoxide dismutase (SOD) proteins, including SodA, SodB and SodC, were selected for analysis. As expected, the yields and the solubility of Tat-tagged proteins were higher than those of Tat-free proteins, and similar results were observed for the total SOD enzyme activity. Bacterial cells that overexpressed Tat-tagged proteins exhibited increased anti-paraquat activity compared with those expressing Tat-free proteins that manifested as SodA4SodC4SodB. When compared with an MG1655 wild-type strain, the growth of a ΔSodA mutant strain was found to be inhibited after paraquat treatment; the growth of ΔSodB and ΔSodC mutant strains was also slightly inhibited. The mRNA transcript level of genes encoding Tat-tagged proteins was higher than that of genes encoding Tat-free proteins. Furthermore, the α-helix and turn of Tat-tagged proteins were higher than those of Tat-free proteins, but the β-sheet and random coil content was lower. These results indicated that the incorporation of the Tat core peptide as a significant basic membrane transduction peptide in fusion proteins could increase mRNA transcripts and promote the high yield and soluble expression of heterologous proteins in E. coli.
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Lingling Fu,Dunmin Lin,Qiaoji Zheng,Xiaochun Wu,Lang Wu,Hailing Sun,Yang Wan,Ximing Fan,Chenggang Xu 한국물리학회 2012 Current Applied Physics Vol.12 No.6
Lead-free ceramics (1-x)NaNbO3-xBi0.5Li0.5TiO3 have been fabricated by an ordinary sintering technique,and their electric properties and temperature characteristics have been studied. All the ceramics possess a perovskite structure with orthorhombic symmetry, indicating that (Bi0.5Li0.5)TiO3 diffuses into NaNbO3 lattices to form a new solid solution. A low (Bi0.5Li0.5)TiO3 doping level transforms the NaNbO3ceramics from antiferroelectric to ferroelectric. The ceramics with x ≤ 0.075 are normal ferroelectric, and the ferroelectric-paraelectric phase become diffusives with the doping level of Bi0.5Li0.5TiO3 increasing. As x increases, the Curie temperature of the ceramics decreases linearly, while the relative permittivity εr increases. 0.925NaNbO3e0.075(Bi0.5Li0.5)TiO3 ceramic exhibits the relatively large piezoelectric constant (d33 = 58 pC/N), high Curie temperature (TC = 228 ℃) and good temperature stability, suggesting that the ceramics are one of new possible candidates for lead-free piezoelectric materials. Lead-free ceramics (1-x)NaNbO3-xBi0.5Li0.5TiO3 have been fabricated by an ordinary sintering technique,and their electric properties and temperature characteristics have been studied. All the ceramics possess a perovskite structure with orthorhombic symmetry, indicating that (Bi0.5Li0.5)TiO3 diffuses into NaNbO3 lattices to form a new solid solution. A low (Bi0.5Li0.5)TiO3 doping level transforms the NaNbO3ceramics from antiferroelectric to ferroelectric. The ceramics with x ≤ 0.075 are normal ferroelectric, and the ferroelectric-paraelectric phase become diffusives with the doping level of Bi0.5Li0.5TiO3 increasing. As x increases, the Curie temperature of the ceramics decreases linearly, while the relative permittivity εr increases. 0.925NaNbO3e0.075(Bi0.5Li0.5)TiO3 ceramic exhibits the relatively large piezoelectric constant (d33 = 58 pC/N), high Curie temperature (TC = 228 ℃) and good temperature stability, suggesting that the ceramics are one of new possible candidates for lead-free piezoelectric materials.
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Quanjun Yang,Xiaolan Cui,Bin Wu,Yujing Shi,Xiaowei Du,Mingsong Fan,Zhaolin Sun,Chenggang Huang 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.1
Bioassay-guided fractionation of extracts from Fructus Gardeniae led to analysis of its bioactive natural products. After infection by influenza virus strain A/FM/1/47-MA in vivo,antiviral activity of the extracts were investigated. The target fraction was orally administered to rats and blood was collected. High-performance liquid chromatography coupled with photo diode array detector and electrospray ion trap multiple-stage tandem mass spectrometry was applied to screen the compounds absorbed into the blood. A structural characterization based on the retention time, ultraviolet spectra, parent ions and fragmentation ions was performed. Thirteen compounds were confirmed or tentatively identified. This provides an accurate profile of the composition of bioactive compounds responsible for the anti-influenza properties.
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