Installing Logic Gates in Permeability Controllable Polyelectrolyte-Carbon Nitride Films for Detecting Proteases and Nucleases

Chen, Lichan,Zeng, Xiaoting,Dandapat, Anirban,Chi, Yuwu,Kim, Donghwan American Chemical Society 2015 ANALYTICAL CHEMISTRY - Vol.87 No.17

<P>Proteases and nucleases are enzymes heavily involved in many important biological processes, such as cancer initiation, progression, and metastasis; hence, they are indicative of potential diagnostic biomarkers. Here, we demonstrate a new label free and sensitive electrochemiluminescent (ECL) sensing strategy for protease and nuclease assays that utilize target-triggered desorption of programmable polyelectrolyte films assembled on graphite-like carbon nitride (g-C<SUB>3</SUB>N<SUB>4</SUB>) film to regulate the diffusion flux of a coreactant. Furthermore, we have built Boolean logic gates OR and AND into the polyelectrolyte films, capable of simultaneously sensing proteases and nucleases in a complicated system by breaking it into simple functions. The developed intelligent permeability controlled enzyme sensor may prove valuable in future medical diagnostics.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2015/ancham.2015.87.issue-17/acs.analchem.5b01916/production/images/medium/ac-2015-01916q_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac5b01916'>ACS Electronic Supporting Info</A></P>

Preimplantation Genetic Testing of Achondroplasia by Two Haplotyping Systems: Short Tandem Repeats and Single Nucleotide Polymorphism

Xiaoting Shen,DongJia Chen,Yan Xu,Yu Fu,Canquan Zhou 한국바이오칩학회 2019 BioChip Journal Vol.13 No.2

Here, we present the successful application of two different preimplantation genetic testing for monogenic diseases (PGT-M) methods for a couple facing the genetic risk of Achondroplasia (ACH). The first preimplantation genetic haplotyping (PGH) cycle was based on short tandem repeats (STRs) and 8 STRs were chosen. The multiple displacement amplification (MDA) products were analyzed using the informative STR loci and PCR-restriction enzyme digestion of FGFR3. A healthy girl was delivered. Two years later, we performed the second PGT-M cycle for this couple with a newly established PGT-M platform based on next generation sequencing (NGS). Haplotype analysis was established by a selection of several informative single nucleotide polymorphisms (SNPs). Preimplantation genetic testing for aneuploidy (PGT-A) was also performed on embryos with normal FGFR3 genotype. Another healthy girl was born. PGH system could be established using STRs or NGS-SNP systems. The NGS-SNP system could detect more sites and simultaneously performs PGT-A with an automated operation.

Next‑Generation Sequencing‑Based Preimplantation Genetic Testing for De Novo NF1 Mutations

DongJia Chen,Xiaoting Shen,Yan Xu,Bing Cai,Chenhui Ding,Yiping Zhong,Yanwen Xu,Canquan Zhou 한국바이오칩학회 2021 BioChip Journal Vol.15 No.1

Neurofibroma type 1 (NFI) is an autosomal dominant genetic disease associated with cancer and cognitive dysfunction. The 50% de novo mutation rate and numerous mutation types of the NF1 gene pose challenges to preimplantation genetic testing (PGT) for NFI. In 2020, a couple was subjected to next-generation sequencing (NGS)-based PGT at our center. Both husband and wife carried de novo NF1 mutations. Biopsied trophectoderm samples underwent whole-genome amplification using multiple displacement amplification. NGS was conducted to detect NF1 mutations as well as 105 closely linked single-nucleotide polymorphisms (SNPs) flanking NF1. Since no affected family member was available, the affected embryos identified by NF1-targeted sequencing were assigned as probands, based on which a haplotype analysis was constructed with informative SNP sites. Sanger sequencing was administrated for verification. Aneuploidy screening through NGS was performed in the same PGT cycle. Finally, among the nine biopsied blastocysts, two unaffected and euploid blastocysts were obtained in the couple, and one of the blastocysts was transferred in a frozen–thawed embryo transfer (FET) cycle. The outcome of FET is under observation. This study is the first to apply NGS-based PGT for NFI. In cases of sporadic NFI cases without affected family members, NGS-SNP can perform haploid analysis in an accurate manner by assigning the affected embryos as probands.

Interference of periostin attenuates pathological changes, proinflammatory markers and renal fibrosis in diabetic kidney injury

Duan Xiaoting,Chen Cheng,Liu Xiaoli,Wang Taoxia,Feng Shuning,Li Jianwei,Li Guiying 한국유전학회 2023 Genes & Genomics Vol.45 No.11

Background Diabetic nephropathy (DN) is a prevalent complication of diabetes, in which inflammation and fibrosis are the significant pathogenesis. Periostin is a matricellular protein that functions on stabilizing the extracellular matrix by binding to integrins during development. This study aimed to explored the role of periostin in DN. Methods The animal and cell models of DN were constructed in streptozocin (STZ)-induced mice and high glucose-challenged human mesangial cells (HMCs). The role of periostin in pathological changes, inflammation and fibrosis in DN was investigated through biochemical detection, HE and Masson staining and scores, western blot, enzyme‑linked immunosorbent assay (ELISA) and real-time quantitative PCR (RT-qPCR) assays. Results Knockdown of periostin counteracted the STZ-induced the ratio of kidney weight and body weight, and the concentrations of urine albumin excretion (UAE), serum creatinine (Scr), urine albumin/creatinine ratio (UACR) and blood urea nitrogen (BUN) in mice. Moreover, silencing of periostin alleviated the pathological manifestations and reduced the concentrations of IL-6, TNF-α and IL-1β in mice kidney tissues and sera. Also, downregulation of periostin decreased the relative protein expression of fibronectin, collagen IV and α-SMA in kidney tissues. Meanwhile, interference of periostin attenuated the levels of pro-inflammation factors and the expressions of fibrosis markers in HG-induced HMCs. Conclusion Interference of periostin resisted DN via attenuating the pro-inflammatory cytokines release and renal fibrosis in diabetic kidney injury. Our study establishes a basis for its further study and underlying application in clinical practice in diagnosing and treating diabetic kidney injury or other relevant diseases.

Diagnosing Balamuthia mandrillaris amebic meningoencephalitis in a 64-year-old woman from the Southwest of China

Yao Suhua,Chen Xiaoting,Qian Lian,Sun Shizheng,Zhao Chunjing,Bai Zongkai,Chen Zhaofang,Wu Youcong 대한기생충학ㆍ열대의학회 2023 The Korean Journal of Parasitology Vol.61 No.2

Balamuthia mandrillaris amebic encephalitis (BAE) can cause a fatal condition if diagnosis is delayed or effective treatment is lacking. Patients with BAE have been previously reported in 12 provinces of China, with skin lesions being the primary symptom and encephalitis developing after several years. However, a significantly lower number of cases has been reported in Southwest China. Here we report an aggressive BAE case of a 64-year-old woman farmer with a history of skin lesions on her left hand. She was admitted to our hospital due to symptoms of dizziness, headache, cough, vomiting, and gait instability. She was initially diagnosed with syphilitic meningoencephalitis and received a variety of empirical treatment that failed to improve her symptoms. Finally, she was diagnosed with BAE combined with amebic pneumonia using next-generation sequencing (NGS), qRT-PCR, sequence analysis, and imaging studies. She died approximately 3 weeks after the onset. This case highlights that the rapid development of encephalitis can be a prominent clinical manifestation of Balamuthia mandrillaris infection.

Study on the Isomerization of Maleic Acid to Fumaric Acid without Catalyst

Zhuo Gao,Wangmi Chen,Xiaoting Chen,Dali Wang,Shouzhi Yi 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.8

Fumaric acid is an important food additive and industrial intermediate compound. The traditional methods of producing fumaric acid were catalyzed by maleic acid isomerization. In this study, isomerization of maleic acid in water without catalyst was investigated at elevated temperature, which addressed the problem of sewage discharge. HPLC analysis showed maleic acid converted into fumaric acid and small amount of malic acid simultaneously. The effects of concentration of maleic acid, reaction temperature, reaction time, and stirring on the yield of fumaric acid were investigated. The optimum reaction conditions were also explored. The results showed the isomerization of maleic acid reached equilibrium at about 1?h, stirring did not affect the reaction rate, and conversion due to the monomolecular mechanism. In order to achieve ?zero emission,? the recycle of filter liquor was also studied. The pH of filter liquor changing signifies the lower pH was in favor of the conversion of maleic acid to fumaric acid. The high yield and recyclability suggested that this process had promising application in fumaric acid production.

Dynamics Modelling and Simulating of Ultra-precision Fly-Cutting Machine Tool

Hanjing Lu,Yuanyuan Ding,Yu Chang,Gangli Chen,Xiaoting Rui 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.2

Dynamics modelling and simulating are the significant process to improve the machining accuracy of the machine tool. This paper is aimed to model and simulate the ultra-precision fly-cutting machine tool (UFMT) and find the relations between structure parameters and machined surface. In this paper, the multi-rigid-flexible-body dynamics model of the UFMT is firstly built by using transfer matrix method for multibody systems. After deducing overall transfer equation, overall transfer matrix, eigenfrequency equation and dynamics equations, the vibration characteristics and dynamics response of tool-tip are simulated and validated by tests. The machined surface is simulated by transferring displacement between the fly-cutting tool-tip and the workpiece into 3D curve. According to the simulation results, both the air-bearing stiffness of the flying-cutting head and cutting process parameters have effects on the machined surface.

Disease burden of prostate cancer from 2014 to 2019 in the United States: estimation from the Global Burden of Disease Study 2019 and Medical Expenditure Panel Survey

Shen Lin,Dong Lin,Yiyuan Li,Lixian Zhong,Wei Zhou,Yajing Wu,Chen Xie,Shaohong Luo,Xiaoting Huang,Xiongwei Xu,Xiuhua Weng 한국역학회 2023 Epidemiology and Health Vol.45 No.-

OBJECTIVES: The aim of this study was to evaluate the disease burden of prostate cancer (PC) and assess key influencing factors associated with the disease expenditures of PC in the United States. METHODS: The total deaths, incidence, prevalence, and disability-adjusted life-years of PC were obtained from the Global Burden of Disease Study 2019. The Medical Expenditure Panel Survey was used to estimate healthcare expenditures and productivity loss and to investigate patterns of payment and use of healthcare resources in the United States. A multivariable logistic regression model was conducted to identify key factors influencing expenditures. RESULTS: For patients aged 50 and older, the burden for all age groups showed a modest increase over the 6-year period. Annual medical expenditures were estimated to range from US$24.8 billion to US$39.2 billion from 2014 to 2019. The annual loss in productivity for patients was approximately US$1,200. The top 3 major components of medical costs were hospital inpatient stays, prescription medicines, and office-based visits. Medicare was the largest source of payments for survivors. In terms of drug consumption, genitourinary tract agents (57.0%) and antineoplastics (18.6%) were the main therapeutic drugs. High medical expenditures were positively associated with age (p=0.005), having private health insurance (p=0.016), more comorbidities, not currently smoking (p=0.001), and patient self-perception of fair/poor health status (p<0.001). CONCLUSIONS: From 2014 to 2019, the national real-world data of PC revealed that the disease burden in the United States continued to increase, which was partly related to patient characteristics.

Disease burden of prostate cancer from 2014 to 2019: estimation from the Global Burden of Disease Study 2019 and Medical Expenditure Panel Survey

Lin Shen,Lin Dong,Li Yiyuan,Zhong Lixian,Zhou Wei,Wu Yajing,Xie Chen,Luo Shaohong,Huang Xiaoting,Xu Xiongwei,Weng Xiuhua 한국역학회 2023 Epidemiology and Health Vol.45 No.-

OBJECTIVES The aim of this study was to evaluate the disease burden of prostate cancer (PC) and assess key influencing factors associated with the disease expenditures of PC in the United States. METHODS The total deaths, incidence, prevalence, and disability-adjusted life-years of PC were obtained from the Global Burden of Disease Study 2019. The Medical Expenditure Panel Survey was used to estimate healthcare expenditures and productivity loss and to investigate patterns of payment and use of healthcare resources in the United States. A multivariable logistic regression model was conducted to identify key factors influencing expenditures. RESULTS For patients aged 50 and older, the burden for all age groups showed a modest increase over the 6-year period. Annual medical expenditures were estimated to range from US$24.8 billion to US$39.2 billion from 2014 to 2019. The annual loss in productivity for patients was approximately US$1,200. The top 3 major components of medical costs were hospital inpatient stays, prescription medicines, and office-based visits. Medicare was the largest source of payments for survivors. In terms of drug consumption, genitourinary tract agents (57.0%) and antineoplastics (18.6%) were the main therapeutic drugs. High medical expenditures were positively associated with age (p=0.005), having private health insurance (p=0.016), more comorbidities, not currently smoking (p=0.001), and patient self-perception of fair/poor health status (p<0.001). CONCLUSIONS From 2014 to 2019, the national real-world data of PC revealed that the disease burden in the United States continued to increase, which was partly related to patient characteristics.