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- 저자 : Charles A Powell (검색결과 4 건)
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Concentric X-Braced Frames with HSS Bracing
Keith D. Palmer,Charles W. Roeder,Dawn E. Lehman,Taichiro Okazaki,Carol K. Shield,Jacob Powell 한국강구조학회 2012 International Journal of Steel Structures Vol.12 No.3
Concentrically braced frames are stiff, strong systems frequently used to resist wind and seismic loading; in regions of high seismicity in the US special concentrically braced frames (SCBFs) are used. CBF configurations vary, but in low rise or other structures with modest levels of demands single-story, X-configured braced frames (X-braced) are commonly used. The brace sections used also vary but hollow structural sections (HSS) are the most common in the U.S. Although important, in part because low-rise structures sustain large demands, few research programs have focused on the single-story X-brace configuration. A large research program was undertaken to understand and improve the response of SCBFs with selected testing on single-story X braced SCBFs. The test matrix consisted of two, full-scale planar X-braced frame experiments and one nearlyfull-scale three-dimensional X-braced frame. The tests were designed using a new design and detailing philosophy, called the Balanced Design Method. In this paper, application of this design method to the frame is investigated, with a focus on the center-splice connection. The results show that the ultimate inelastic deformation capacity of the system is less dependent on the specific design detail at this splice. Additionally, the bi-directional load testing indicated that the out-of-plane demands did not impact the system performance.
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최철우,현재욱,황록연,Charles A Powell 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.6
Huanglongbing (HLB, Citrus greening disease) is one of the most devastating diseases that threaten citrus production worldwide. Although HLB presents systemically, low titer and uneven distribution of these bacteria within infected plants can make reliable detection difficult. It was known loop-mediated isothermal amplification (LAMP) method has the advantages of being highly specific, rapid, efficient, and laborsaving for detection of plant pathogens. We developed a new LAMP method targeting gene contained tandem repeat for more rapid and sensitive detection of Candidatus Liberibacter asiaticus (CLas), putative causal agent of the citrus huanglongbing. This new LAMP method was 10 folds more sensitive than conventional PCR in detecting the HLB pathogen and similar to that of real-time PCR in visual detection assay by adding SYBR Green I to mixture and 1% agarose gel electrophoresis. Positive reactions were achieved in reaction temperature 57, 60 and 62oC but not 65oC. Although this LAMP method was not more sensitive than real-time PCR, it does not require a thermocycler for amplification or agarose gel electrophoresis for resolution. Thus, we expect that this LAMP method shows strong promise as a reliable, rapid, and cost-effective method of detecting the CLas in citrus and can be applied for rapid diagnosis is needed.
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Choi, Cheol Woo,Hyun, Jae Wook,Hwang, Rok Yeon,Powell, Charles A The Korean Society of Plant Pathology 2018 Plant Pathology Journal Vol.34 No.6
Huanglongbing (HLB, Citrus greening disease) is one of the most devastating diseases that threaten citrus production worldwide. Although HLB presents systemically, low titer and uneven distribution of these bacteria within infected plants can make reliable detection difficult. It was known loop-mediated isothermal amplification (LAMP) method has the advantages of being highly specific, rapid, efficient, and laborsaving for detection of plant pathogens. We developed a new LAMP method targeting gene contained tandem repeat for more rapid and sensitive detection of Candidatus Liberibacter asiaticus (CLas), putative causal agent of the citrus huanglongbing. This new LAMP method was 10 folds more sensitive than conventional PCR in detecting the HLB pathogen and similar to that of real-time PCR in visual detection assay by adding SYBR Green I to mixture and 1% agarose gel electrophoresis. Positive reactions were achieved in reaction temperature 57, 60 and $62^{\circ}C$ but not $65^{\circ}C$. Although this LAMP method was not more sensitive than real-time PCR, it does not require a thermocycler for amplification or agarose gel electrophoresis for resolution. Thus, we expect that this LAMP method shows strong promise as a reliable, rapid, and cost-effective method of detecting the CLas in citrus and can be applied for rapid diagnosis is needed.
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Ruchi Bansal,Shigeki Nakagawa,Saleh Yazdani,Joop van Baarlen,Anu Venkatesh,Anna P Koh,송원민,Nicolas Goossens,Hideo Watanabe,Mary B Beasley,Charles A Powell,Gert Storm,Naftali Kaminski,Harry van Goor,Sco 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Tissue fibrosis, characterized by excessive accumulation of aberrant extracellular matrix (ECM) produced by myofibroblasts, is a growing cause of mortality worldwide. Understanding the factors that induce myofibroblastic differentiation is paramount to prevent or reverse the fibrogenic process. Integrin-mediated interaction between the ECM and cytoskeleton promotes myofibroblast differentiation. In the present study, we explored the significance of integrin alpha 11 (ITGA11), the integrin alpha subunit that selectively binds to type I collagen during tissue fibrosis in the liver, lungs and kidneys. We showed that ITGA11 was co-localized with α-smooth muscle actin-positive myofibroblasts and was correlatively induced with increasing fibrogenesis in mouse models and human fibrotic organs. Furthermore, transcriptome and protein expression analysis revealed that ITGA11 knockdown in hepatic stellate cells (liver-specific myofibroblasts) markedly reduced transforming growth factor β-induced differentiation and fibrotic parameters. Moreover, ITGA11 knockdown dramatically altered the myofibroblast phenotype, as indicated by the loss of protrusions, attenuated adhesion and migration, and impaired contractility of collagen I matrices. Furthermore, we demonstrated that ITGA11 was regulated by the hedgehog signaling pathway, and inhibition of the hedgehog pathway reduced ITGA11 expression and fibrotic parameters in human hepatic stellate cells in vitro, in liver fibrosis mouse model in vivo and in human liver slices ex vivo. Therefore, we speculated that ITGA11 might be involved in fibrogenic signaling and might act downstream of the hedgehog signaling pathway. These findings highlight the significance of the ITGA11 receptor as a highly promising therapeutic target in organ fibrosis.
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