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- 저자 : Bu-Youn Kim (검색결과 79 건)
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비만 치료 및 예방에 대한 한약의 효능 연구(Ⅱ) : 4종 처방이 비만 흰쥐의 면역조직화학적 변화에 미치는 영향
金蓮燮,卞晟僖,徐富一,金相贊,國萬,趙應行 대한본초학회 2000 大韓本草學會誌 Vol.15 No.1
We had experiment with several prescriptions in order to find cure and prevention effects for an obesity. We fed control gmup rats high fat diet and administered normal saline for Bweeb. And we fed experimental gmup ram high fat diet and administered exmct of four prescriptions for Bweek. The result were as follows: 1. The size of the epididymal fat cell of rats in Bikam-whan, Chekamhanghyuluiyiin-rang and Chekambohyulansin-tang groups showed a significant decrease in comparison with control group. 2. The fat drops area in hepatic lobule of rats in Bikam-whan, Cheknmhanghyuluiyiin-rang, Chekambohyulonrin-tang, and Chekambangpungrongseong-san groups showed a significant decrease in comparison with control group. 3. The area of the insulin granuls in islet of rats in Bikam-who4 Chekamhnnghyuluiyiin-tang and Chekambohyulonrin-tong group showed a significant decrease in comparison with control group. According to above these results, Bikam-whan. Cheknmhonghyuluiviin-tnng and Chekambohyulonrin-tang have shown to be capable of cure and prevent for an obesty.
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갈근이 뇌허혈 손상 흰쥐의 해마신경세포 손상에 미치는 영향
부일권,김연섭 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1
Objective : This study investigated a neuroprotective effect of herariae Radix on cerebral ischemia. Method : The global cerebral ischemia was induced by bilateral common carotid arteries occlusion under hypotension (40 mmHg) in Sprague-Dawley rats. After the treatment of Puerariae Radix extract, changes of the pyramidal neurons in CAl region of the hippocampus were observed histologically. Results :l. The significant decrease of numbers of pyramidal neurons in CA1 region of the hippocampus resulted by cerebral ischemia. Then herariae Radix treatment demonstrated significant increase of numbers of pyramidal neurons in CA1 region as compared with control group. 2. The significant decrease of pyramidal layer thickness in CA1 region of the hippocampus resulted by cerebral ischemia. Then herariae Radix treatment demonstrated significant increase of pyramidal layer thickness in CAI region as compared with control group. Conclusion : These results suggested that Puerariae Radix reveals the neuroprotective effect through the control of noxious stress stimulations to neurons.
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천안시 도로변의 NO₂및 SO₂오염도 측정에 관한 연구
손부순,염윤기,김종오,문정숙 한양대학교 환경및산업의학연구소 2000 環境과 産業醫學 Vol.9 No.1
This study's goale to find out the degree of the air pollution caused by vehicle emissions and to collect basic data for alternative plan in the improvement of the quality of the air by searching for the quality of the air around the road(in front of Chunan station, Terminal intersection, at Bangiugan five forked road, Chunanro intersection) in Chunan. NO2 and SO2 were measured as a object for this study in winter time and in summer time respectively. There has been an rapid increase in population and industrial complex has been built up in Chunan area. Therefore an increase in the number of vehicles and the use of domestic fuel has followed by that. So more studies of that and the effort for the improvement in the quality of the air will have to be made.
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(Zn, Cd)S:Cu 형광체의 발광(PL, CL) 현상에 관한 연구
신영진,尹昌周,金富吉 全北大學校 基礎科學硏究所 1979 基礎科學 Vol.2 No.1
Photoluminescent and cathodoluminescent (Zn, Cd)S phosphors are prepared by firing at 850℃ in N_2 gas of atmospheric pressure for an hour. They are doped with (1) an activator CuS, (2) an activator CuCl, (3) a flux NH_4Cl, (4) both an activator CuCl and the flux NH_4Cl, an (5) not doped with either one. The crystal structure of ZnS phosphors powder is the mixed phase of cubic and haxagonal, while the powders of (Zn, Cd)S : Cu (Zn, Cd)S : Cu, Cl and (Zn, Cd)S phosphors are the hexagonal pattern under the same firing condition. Photoluminescent and cathodoluminescent cells are made with these phorsphors and their emission spectra are measured at room temperature after these materials are excited with 3650 A˚ light and cathode rays. These spectra are shifted from blue (green) to red wavelength as the CdS content of the phosphors is increased, The NH_4Cl flux is found to help the doping of the impurity energy level and it has SA center in SA emisson. The following two results are also observed about (Zn, Cd) : Cu;(1) the values of lattice parameter a are varied linearly with the molar fraction of (Zn,Cd) (2) the impurity changes with quadratic curve when the molar fraction is varied.
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Nur77 upregulates HIF-α by inhibiting pVHL-mediateddegradation
Bu-Yeon Kim,Eun-Jung Cho,Hong-Duk Youn,Hyungsoo Kim 생화학분자생물학회 2008 Experimental and molecular medicine Vol.40 No.1
In this study, we investigated the role of Nur77, an orphan nuclear receptor, in HIF-α transcriptional activity. We found that Nur77 associates and stabilizes HIF-1α via indirect interaction. Nur77 was found to interact with pVHL in vivo via the α-domain of pVHL. By binding to pVHL, Nur77 competed with elongin C for pVHL binding. Moreover, Nur77-binding to pVHL inhibited the pVHL-mediated ubiquitination of HIF-1α and ultimately increased the stability and transcriptional activity of HIF-1α. The ligand-binding domain of Nur77 was found to interact with pVHL and the expression of this ligand-binding domain was sufficient to stabilize and transactivate HIF-1α. Under the conditions that cobalt chloride was treated or pVHL was knocked down, Nur77 could not stabilize HIF-α. Moreover, Nur77 could not further stabilize HIF-2α in A498/VHL stable cells, which is consistent with our finding that Nur77 indirectly stabilizes HIF-α by binding to pVHL. Thus, our results suggest that an orphan nuclear receptor Nur77 binds to pVHL, thereby stabilizes and increases HIF-α transcriptional activity under the nonhypoxic and ultimately increased the stability and transcriptional activity of HIF-1α. The ligand-binding domain of Nur77 was found to interact with pVHL and the expression of this ligand-binding domain was sufficient to stabilize and transactivate HIF-1α. Under the conditions that cobalt chloride was treated or pVHL was knocked down, Nur77 could not stabilize HIF-α. Moreover, Nur77 could not further stabilize HIF-2α in A498/VHL stable cells, which is consistent with our finding that Nur77 indirectly stabilizes HIF-α by binding to pVHL. Thus, our results suggest that an orphan nuclear receptor Nur77 binds to pVHL, thereby stabilizes and increases HIF-α transcriptional activity under the nonhypoxic conditions. In this study, we investigated the role of Nur77, an orphan nuclear receptor, in HIF-α transcriptional activity. We found that Nur77 associates and stabilizes HIF-1α via indirect interaction. Nur77 was found to interact with pVHL in vivo via the α-domain of pVHL. By binding to pVHL, Nur77 competed with elongin C for pVHL binding. Moreover, Nur77-binding to pVHL inhibited the pVHL-mediated ubiquitination of HIF-1α and ultimately increased the stability and transcriptional activity of HIF-1α. The ligand-binding domain of Nur77 was found to interact with pVHL and the expression of this ligand-binding domain was sufficient to stabilize and transactivate HIF-1α. Under the conditions that cobalt chloride was treated or pVHL was knocked down, Nur77 could not stabilize HIF-α. Moreover, Nur77 could not further stabilize HIF-2α in A498/VHL stable cells, which is consistent with our finding that Nur77 indirectly stabilizes HIF-α by binding to pVHL. Thus, our results suggest that an orphan nuclear receptor Nur77 binds to pVHL, thereby stabilizes and increases HIF-α transcriptional activity under the nonhypoxic and ultimately increased the stability and transcriptional activity of HIF-1α. The ligand-binding domain of Nur77 was found to interact with pVHL and the expression of this ligand-binding domain was sufficient to stabilize and transactivate HIF-1α. Under the conditions that cobalt chloride was treated or pVHL was knocked down, Nur77 could not stabilize HIF-α. Moreover, Nur77 could not further stabilize HIF-2α in A498/VHL stable cells, which is consistent with our finding that Nur77 indirectly stabilizes HIF-α by binding to pVHL. Thus, our results suggest that an orphan nuclear receptor Nur77 binds to pVHL, thereby stabilizes and increases HIF-α transcriptional activity under the nonhypoxic conditions.
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Production of Acrylamide Using Immobilized Cells of Rhodococcus rhodochrous M33
Kim, Bu-Youn,Hyun, Hyung-Hwan The Korean Society for Biotechnology and Bioengine 2002 Biotechnology and Bioprocess Engineering Vol.7 No.4
The cells of Rhodococcus rhodochrous M33, which produce a nitrile hydratase enzyme, were immobilized in acrylamide-based polymer gels. The optimum pH and temperature for the activity of nitrile hydratase in both the free and Immobilized cells were 7.4 and 45$\^{C}$, respectively, yet the optimum temperature for acrylamide production by the immobilized cells was 20$\^{C}$. The nitrile hydratase of the immobilized cells was more stable with acrylamide than that of the free cells. Under optimal conditions, the final acrylamide concentration reached about 400 g/L with a conversion yield of almost 100% after 8 h of reaction when using 150 g/L of immobilized cells corresponding to a 1.91 g-dry cell weight/L. The enzyme activity of the immobilized cells rapidly de-creased with repeated use. However, the quality of the acrylamide produced by the immobilized cells was much better than that produced by the free cells in terms of color, salt content, turbidity, and foam formation. The quality of the aqueous acrylamide solution obtained was found to be of commercial use without further purification.
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Fed-batch Fermentation for Production of Nitrile Hydratase by Rhodococcus rhodochrous M33
Kim, Bu-Youn,Kim, Jong-Chul,Lee, Hyune-Hwan,Hyun, Hyung-Hwan The Korean Society for Biotechnology and Bioengine 2001 Biotechnology and Bioprocess Engineering Vol.6 No.1
To enhance the productivity and activity of nitrile hydratase in Rhodococcus rhodochrous M33, a glucose-limited fed-batch culture was performed. In a fed-batch culture where the glucose was controlled at a limited level and cobalt was supplemented during the fermentation period, the cell mass and total activity of nitrile hydratase both increased 3.3-fold compared to that in the batch fermentation. The productivity of nitrile hydratase also increased 1.9-fold compared to that in the batch fermentation. The specific activity of nitrile hydratase in the whole cell preparation when using a fed-batch culture was 120 units/mg-DCW, which was similar to that in the batch culture.
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( Hyung-seop Youn ),( Tae Gyun Kim ),( Mun-kyoung Kim ),( Gil Bu Kang ),( Jung Youn Kang ),( Jung-gyu Lee ),( Jun Yop An ),( Kyoung Ryoung Park ),( Youngjin Lee ),( Young Jun Im ),( Jun Hyuck Lee ),( 전남대학교 약품개발연구소 2016 약품개발연구지 Vol.25 No.-
Ouinolinate phosphoribosyltransferase (QPRT) catalyses the production of nicotinic acid Mononucleotide, a precursor of de novo biosynthesis of the ubiquitous coenzyme nicotinamide adenine dinucleotide. QPRT is also essential for maintaining the homeostasis of quinolinic acid in the brain, a possible neurptoxin causing various neurodegenerative diseases. Although QPRT has been extensively analysed, the molecular basis of the reaction catalyzed by human QPRT remains unclear. Here, we present the crystal structures of hexameric human QPRT in the apo form and its complexes With reactant or product. We found that the interaction between dimeric subunits was dramatically altered during the reaction process by conformational changes of two flexible loops in the active site at the dimer-dimer interface. In addition, the N-terminal short helix α1 was identified as a critical hexamer stabilizer. The structural features, size distribution, heat aggregation and ITC studies of the full-length enzyme and the enzyme lacking helix α1 stroungly suggest that human QPRT acts as a hexamer for cooperative reactant binding via three dimeric subunits and maintaining stability. Based on our comparison of human QPRT structures in the apo and complex forms, we propose a drug design strategy targeting malignant glioma.
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