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무작위 염기서열법에 의한 미성숙 벼종자의 cDNA 분석
피봉관,한태룡 경희대학교 유전공학연구소 1995 遺傳工學論文集 Vol.7 No.-
Partial nucleotide sequences(150∼300 bp) of randomly selected cDNA clones from cDNA library constructed with immature rice seeds have been determined. The nucleotide sequences of 200 clones were analyzed with the databases of EMBL and Gen-Bank. The results showed that 83 cDNA clones were identical to known rice genes, 17 and 4 cDNA clones were homologous with other plant and nonplant genes, respectively. Among cDNAs identical with known rice genes, 35 clones were identified as glutelin, 26 clones as prolamin, and one clone as globulin genes, respectively. Northern blot analyses suggest that all the storage protein genes were mainly expressed in immature seeds, but not in leaves, stems, roots, or young seedlings. ADP-glucose pyrophosphorylase gene which is involved in starch synthesis was also expressed only at the early stage of rice seed formation.
Phee, Bong-Kwan,Shin, Dong Ho,Cho, Jin-Hwan,Kim, Seong-Hee,Kim, Jeong-Il,Lee, Youn-Hyung,Jeon, Jong-Seong,Bhoo, Seong Hee,Hahn, Tae-Ryong WILEY-VCH 2006 Proteomics Vol. No.
<P>Phytochrome-interacting proteins have been extensively studied to elucidate light-signaling pathway in plants. However, most of these proteins have been identified by yeast two-hybrid screening using the C-terminal domain of phytochromes. We used co-immunoprecipitation followed by proteomic analysis in plant cell extracts in an attempt to screen for proteins interacting either directly or indirectly with native holophytochromes including the N-terminal domain as well as C-terminal domain. A total of 16 protein candidates were identified, and were selected from 2-DE experiments. Using MALDI-TOF MS analysis, 7 of these candidates were predicted to be putative phytochrome A-interacting proteins and the remaining ones to be phytochrome B-interacting proteins. Among these putative interacting proteins, protein phosphatase type 2C (PP2C) and a 66-kDa protein were strong candidates as novel phytochrome-interacting proteins, as knockout mutants for the genes encoding these two proteins had impaired light-signaling functions. A transgenic knockout Arabidopsis study showed that a 66-kDa protein candidate regulates hypocotyl elongation in a light-specific manner, and altered cotyledon development under white light during early developmental stages. The PP2C knockout plants also displayed light-specific changes in hypocotyl elongation. These results suggest that co-immunoprecipitation, followed by proteomic analysis, is a useful method for identifying novel interacting proteins and determining real protein-protein interactions in the cell.</P>
Lee, Sang-Won,Jeong, Kyu-Sik,Han, Sang-Wook,Lee, Seung-Eun,Phee, Bong-Kwan,Hahn, Tae-Ryong,Ronald, Pamela American Society for Microbiology 2008 Journal of Bacteriology Vol.190 No.6
<B>ABSTRACT</B><P>The rice pathogen recognition receptor, XA21, confers resistance to <I>Xanthomonas oryzae</I> pv. oryzae strains producing the type one system-secreted molecule, AvrXA21. <I>X. oryzae</I> pv. oryzae requires a regulatory two-component system (TCS) called RaxRH to regulate expression of eight <I>rax</I> (<I>r</I>equired for <I>A</I>vr<I>X</I>A21 activity) genes and to sense population cell density. To identify other key components in this critical regulatory circuit, we assayed proteins expressed in a <I>raxR</I> gene knockout strain. This survey led to the identification of the <I>phoP</I> gene encoding a response regulator that is up-regulated in the <I>raxR</I> knockout strain. Next we generated a <I>phoP</I> knockout strain and found it to be impaired in <I>X. oryzae</I> pv. oryzae virulence and no longer able to activate the response regulator HrpG (hypersensitive reaction and pathogenicity G) in response to low levels of Ca<SUP>2+</SUP>. The impaired virulence of the <I>phoP</I> knockout strain can be partially complemented by constitutive expression of <I>hrpG</I>, indicating that PhoP controls a key aspect of <I>X. oryzae</I> pv. oryzae virulence through regulation of <I>hrpG</I>. A gene encoding the cognate putative histidine protein kinase, <I>phoQ</I>, was also isolated. Growth curve analysis revealed that AvrXA21 activity is impaired in a <I>phoQ</I> knockout strain as reflected by enhanced growth of this strain in rice lines carrying XA21. These results suggest that the <I>X. oryzae</I> pv. oryzae PhoPQ TCS functions in virulence and in the production of AvrXA21 in partnership with RaxRH.</P>
Kim, Hyo Jung,Hong, Sung Hyun,Kim, You Wang,Lee, Il Hwan,Jun, Ji Hyung,Phee, Bong-Kwan,Rupak, Timilsina,Jeong, Hana,Lee, Yeonmi,Hong, Byoung Seok,Nam, Hong Gil,Woo, Hye Ryun,Lim, Pyung Ok Oxford University Press 2014 Journal of experimental botany Vol.65 No.14
<P>Leaf senescence is a finely tuned and genetically programmed degeneration process, which is critical to maximize plant fitness by remobilizing nutrients from senescing leaves to newly developing organs. Leaf senescence is a complex process that is driven by extensive reprogramming of global gene expression in a highly coordinated manner. Understanding how gene regulatory networks involved in controlling leaf senescence are organized and operated is essential to decipher the mechanisms of leaf senescence. It was previously reported that the trifurcate feed-forward pathway involving <I>EIN2</I>, <I>ORE1</I>, and <I>miR164</I> in <I>Arabidopsis</I> regulates age-dependent leaf senescence and cell death. Here, new components of this pathway have been identified, which enhances knowledge of the gene regulatory networks governing leaf senescence. Comparative gene expression analysis revealed six senescence-associated NAC transcription factors (TFs) (ANAC019, AtNAP, ANAC047, ANAC055, ORS1, and ORE1) as candidate downstream components of ETHYLENE-INSENSITIVE2 (EIN2). EIN3, a downstream signalling molecule of EIN2, directly bound the <I>ORE1</I> and <I>AtNAP</I> promoters and induced their transcription. This suggests that EIN3 positively regulates leaf senescence by activating <I>ORE1</I> and <I>AtNAP</I>, previously reported as key regulators of leaf senescence. Genetic and gene expression analyses in the <I>ore1 atnap</I> double mutant revealed that ORE1 and AtNAP act in distinct and overlapping signalling pathways. Transient transactivation assays further demonstrated that ORE1 and AtNAP could activate common as well as differential NAC TF targets. Collectively, the data provide insight into an EIN2-mediated senescence signalling pathway that coordinates global gene expression during leaf senescence via a gene regulatory network involving EIN3 and senescence-associated NAC TFs.</P>