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- 저자 : Bharathiraja, Subramaniyan (검색결과 12 건)
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Chlorin e6 conjugated silica nanoparticles for targeted and effective photodynamic therapy
Bharathiraja, S.,Moorthy, M.S.,Manivasagan, P.,Seo, H.,Lee, K.D.,Oh, J. ELSEVIER SCIENCE B.V., AMSTERDAM 2017 PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY Vol.19 No.-
Photodynamic therapy (PDT) using photosensitizer drug has become an important therapeutic modality. However, the stability and targeted delivery of photosensitizer remain a critical challenge for efficient PDT treatment. In the present study, we developed chlorin e6 (Ce6)-conjugated and folic acid (FA)-decorated silica nanoparticles (silica-Ce6-FA) for targeted delivery of photosensitizer to the cancer cells. The synthesized NPs exhibited excellent stability and biocompatibility with MDA-MB-231 cells. The formulated particles were efficiently taken up by folate receptor-positive MDA-MB-231 cells, which were confirmed by comparative analysis with folate receptor-negative HepG2 cells. The folate receptor-targeted silica-Ce6-FA was highly accumulated inside the MDA-MB-231 cells than free Ce6. The obtained NPs produced singlet oxygen efficiently under 670-nm laser exposure. The cell-killing effect of silica-Ce6-FA was higher when compared with free Ce6 under PDT treatment. The PDT-induced mitochondrial damage and apoptotic cell death were detected in silica-Ce6-FA-treated cells.
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Chlorin e6 conjugated copper sulfide nanoparticles for photodynamic combined photothermal therapy
Bharathiraja, S.,Manivasagan, P.,Moorthy, M.S.,Bui, N.Q.,Lee, K.D.,Oh, J. ELSEVIER SCIENCE B.V., AMSTERDAM 2017 PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY Vol.19 No.-
The photo-based therapeutic approaches have attracted tremendous attention in recent years especially in treatment and management of tumors. Photodynamic and photothermal are two major therapeutic modalities which are being applied in clinical therapy. The development of nanomaterials for photodynamic combined with photothermal therapy has gained significant attention for its treatment efficacy. In the present study, we designed chlorin e6 (Ce6) conjugated copper sulfide (CuS) nanoparticles (CuS-Ce6 NPs) through amine functionalization and the synthesized nanoparticles act as a dual-model agent for photodynamic therapy and photothermal therapy. CuS-Ce6 NPs showed enhanced photodynamic effect through generation of singlet oxygen upon 670nm laser illumination. The same nanoparticles exerted thermal response under an 808nm laser at 2W/cm<SUP>2</SUP>. The fabricated nanoparticles did not show any cytotoxic effect toward breast cancer cells in the absence of light. In vitro cell viability assay showed a potent cytotoxicity in photothermal and photodynamic treatment. Rather than singular treatment, the photodynamic combined photothermal treatment showed an enhanced cytotoxic effect on treated cells. In addition, the CuS-Ce6 NPs exert a photoacoustic signal for non-invasive imaging of treated cells in tissue-mimicking phantom. In conclusion the CuS-Ce6 NPs act as multimodal agent for photo based imaging and therapy.
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Bharathiraja, Subramaniyan,Manivasagan, Panchanathan,Santha Moorthy, Madhappan,Bui, Nhat Quang,Jang, Bian,Phan, Thi Tuong Vy,Jung, Won-Kyo,Kim, Young-Mok,Lee, Kang Dae,Oh, Junghwan Elsevier 2018 EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEU Vol.123 No.-
<P><B>Abstract</B></P> <P>Photodynamic therapy (PDT) and photothermal therapy (PTT) using nanoparticles have gained significant attention for its therapeutic effect for cancer treatment. In the present study, we fabricated polypyrrole nanoparticles by employing bovine serum albumin-phycocyanin complex and the formulated particles were stable in various physiological solutions like water, phosphate buffered saline and culture media. The formulated nanoparticles did not cause any noticeable toxicity to MDA-MB-231 and HEK-293 cells. The obtained nanoparticles effectively killed MDA-MB-231 cells in a dual way upon laser illumination, one is through phycocyanin propagated reactive oxygen species (PDT) upon laser illumination and in another way it eradicated the treated cells by converting optical energy into heat energy (PTT). Additionally, the nanoparticles generated good amplitude of ultrasound signals under photoacoustic imaging (PAT) system that facilitates imaging of treated cells. In conclusion, the fabricated particles could be used as a multimodal therapeutic agent for treatment of cancer in the biomedical field.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Phycocyanin-BSA stabilized polypyrrole nanoparticles (Ppy@BSA-Pc) formulated. </LI> <LI> Ppy@BSA-Pc can be applied to PDT and PTT treatment. </LI> <LI> Ppy@BSA-Pc facilitates imaging of treated cells under PAI system. </LI> <LI> Ppy@BSA-Pc can act as a theranostic agent in biomedical field. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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( Bharathiraja S ),( Selvamuthukumaran S ),( Balaji V ) 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.8
The Wireless Sensor Network (WSN), is constructed out of teeny-tiny sensor nodes that are very low-cost, have a low impact on the environment in terms of the amount of power they consume, and are able to successfully transmit data to the base station. The primary challenges that are presented by WSN are those that are posed by the distance between nodes, the amount of energy that is consumed, and the delay in time. The sensor node's source of power supply is a battery, and this particular battery is not capable of being recharged. In this scenario, the amount of energy that is consumed rises in direct proportion to the distance that separates the nodes. Here, we present a Hybrid Firefly Glow-Worm Swarm Optimization (HF-GSO) guided routing strategy for preserving WSNs' low power footprint. An efficient fitness function based on firefly optimization is used to select the Cluster Head (CH) in this procedure. It aids in minimising power consumption and the occurrence of dead sensor nodes. After a cluster head (CH) has been chosen, the Glow-Worm Swarm Optimization (GSO) algorithm is used to figure out the best path for sending data to the sink node. Power consumption, throughput, packet delivery ratio, and network lifetime are just some of the metrics measured and compared between the proposed method and methods that are conceptually similar to those already in use. Simulation results showed that the proposed method significantly reduced energy consumption compared to the state-of-the-art methods, while simultaneously increasing the number of functioning sensor nodes by 2.4%. Proposed method produces superior outcomes compared to alternative optimization-based methods.
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Manivasagan, Panchanathan,Bharathiraja, Subramaniyan,Santha Moorthy, Madhappan,Oh, Yun-Ok,Song, Kyeongeun,Seo, Hansu,Oh, Junghwan American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.17
<P>The development of novel photothermal ablation agents as cancer nanotheranostics has received a great deal of attention in-recent decades. Biocompatible fucoidan (Fu) is used as the coating material for gold nanorods (AuNRs) and subsequently conjugated with monoclonal antibodies against epidermal growth factor receptor (anti-EGFR) novel photothermal ablation agents for,cancer nanotheranostics because of their excellent biocompatibility, biodegradability, nontoxicity, water solubility, photostability, ease of Surface modification, strongly enhanced absorption in near-infrared (NIR) regions, target specificity, minimal invasiveness, fast recovery, and prevention. of damage to normal tissues: Anti-EGFRFu-AuNRs have an average particle size of 96.37 +/- 3.73 nm. Under 808 nm NIR laser at 2 W/cm(2) for 5 min, the ternperathre of the solution containing anti-EGFR Fu-AuNRs,(30 mu g/mL) increased by 52.1 degrees C. The anti-EGFR Fu-AuNRs exhibited high efficiency for the ablation of MDA-MB-231 cells in vitro. In viva- photothermal ablation exhibited that tumor tissues fully recovered without recurrence and finally were reconstructed with normal-tissues by-the 808 nm NIER. laser irradiatiOn after injection of anti-EGFR Fu-AuNRs. These results suggest that the anti-BUR Fu-AuNRs would be novel photoablation agents for future cancer nanotheranostics:</P>
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Manivasagan, P.,Bharathiraja, S.,Bui, N.Q.,Lim, I.G.,Oh, J. Elsevier/North Holland 2016 International journal of pharmaceutics Vol.511 No.1
Polymer nanoparticles have gained significant attention as potential drug carriers for anticancer agents and molecular imaging. Biocompatible gold nanoparticles (AuNPs) were synthesized using chitosan oligosaccharide (COS) as a reducing and stabilizing agent and were subsequently loaded with paclitaxel (PTX) to demonstrate their use in drug delivery and photoacoustic imaging (PAI) of MDA-MB-231 cells. Paclitaxel-loaded chitosan oligosaccharide-stabilized gold nanoparticles (PTX-COS AuNPs) were spherical in shape with an average particle size of 61.86+/-3.01nm. PTX-COS AuNPs showed sustained and pH-dependent drug release profiles and exhibited strong cytotoxic effect against MDA-MB-231 cells through the induction of apoptosis with improved reactive oxygen species (ROS) generation and altered mitochondrial membrane potential (MMP) level. The cellular internalization of PTX-COS AuNPs was proven by fluorescence microscopy as well as flow cytometry. PTX-COS AuNPs were also evaluated as a new class of optical contrast agents for photoacoustic imaging (PAI). To the best of our knowledge, this is the first report that describes the use of PTX-COS AuNPs as novel agents for drug delivery and PAI of cancer cells. These results exposed the promising potential of PTX-COS AuNPs in the field of drug delivery, molecular imaging, and cancer therapy in the near future.
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Kim, Hyehyun,Mondal, Sudip,Bharathiraja, Subramaniyan,Manivasagan, Panchanathan,Moorthy, Madhappan Santha,Oh, Junghwan Elsevier 2018 CERAMICS INTERNATIONAL Vol.44 No.6
<P><B>Abstract</B></P> <P>The synthesis and fabrication of multifunctional nanostructures with enhanced biocompatibility are the most important characteristics for biomedical research. The goal of our present research is to study the optimum zinc (Zn)-loading on pure hydroxyapatite (HAp) bioceramics and its potential advantages in biomedical application. In this study, different mole concentrations (1, 2, 5mol%) of Zn doped HAp (Zn-HAp) nanoparticles were synthesized through a facile co-precipitation technique using zinc nitrate as a source for Zn metal. The synthesized Zn-HAp nanoparticles were critically characterized for their structural and morphological changes by different spectroscopy and electron microscopy analysis. The potential advances of Zn-HAp nanoparticles in biological application was studied by using MG-63 cell line, drug model experiment and scaffold cell attachment, proliferation study. The cell cytotoxicity test (MTT assay and trypan blue) was first conducted to confirm the nontoxic characteristics of Zn-HAp with enhanced MG-63 cell proliferation activity. The drug loading experiment of Zn-HAp nanoparticles was then confirmed with 1mol% Zn-HAp (which had the maximum drug loading efficiency with pH responsive drug interaction). Furthermore, the optimized 1mol% Zn-HAp constructed biomimetic scaffold shows excellent cell attachment and proliferation behavior with MG-63 cells. The result suggests that the biomimetic 1mol% Zn-HAp scaffolds may be of enormous potential in bone repair and regeneration. This research distinguishes from other research by showing an advanced analysis of the Zn-HAp and its enhanced physicochemical properties for tissue engineering and pH responsive drug delivery application.</P>
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A. Umamageswari,N. Bharathiraja,D. Shiny Irene 한국통신학회 2023 ICT Express Vol.9 No.2
This study proposed a novel framework for plant leaf disease identification. The proposed model consists of four steps including pre-processing, segmentation, feature extraction, and classification. At first, the unwanted noise and overfitting are removed, and also image contrast level is enhanced. Secondly, the Fuzzy C-Means (FCM) based Chameleon Swarm Algorithm (CSA) named as (FCM-CSA) is used for plant leaf diseased part segmentation. In the third stage, the feature extraction is performed using a fast GLCM feature extraction model. Finally, the Progressive Neural Architecture Search (PNAS) is used for plant leaf disease identification. The experimental investigations are carried out using MATLAB software with the Mendeley database. From this dataset, we have used Apple Cedar Apple Rust (ACAR), Cherry Powdery Mildew (CPM), Corn Common Rust (CCCR), Apple Healthy (AH), Grape Black Rot (GBR), Pepper Bell Bacterial Spot (PBBS), Potato Late Blight (PLB) and Tomato Leaf Mold (TLM) disease images. Different measures such as precision, recall, sensitivity, specificity, and accuracy results are used to validate the performance of the proposed model.
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Santha Moorthy, Madhappan,Hoang, Giang,Subramanian, Bharathiraja,Bui, Nhat Quang,Panchanathan, Manivasagan,Mondal, Sudip,Thi Tuong, Vy Phan,Kim, Hyehyun,Oh, Junghwan The Royal Society of Chemistry 2018 Journal of materials chemistry. B, Materials for b Vol.6 No.32
<P>The fabrication of nanotherapeutic systems capable of stimuli-responsive drug delivery and photoacoustic imaging (PAI)-guided photothermal therapy (PTT) is considered significant for chemo-photothermal therapy applications in cancer therapy. In the present study, the Prussian blue nanoflake (PBNF) decorated mesoporous silica hybrid nanoparticle (PB@MSH-EDA NPs) is reported for PAI-guided chemo-photothermal therapy applications. The amine group enriched mesoporous silica channels can be used to encapsulate an anticancer drug for chemotherapy, and the surface decorated PBNFs can convert a near-infrared (NIR) laser (808 nm) into heat for photothermal therapy and can also be used for PAI applications. The PB@MSH-EDA NPs show pH-responsive drug release efficiency under acidic pH (pH 5.0 and 4.0) conditions. Furthermore, the PB@MSH-EDA NPs system shows strong NIR laser absorption and photothermal conversion efficiency under 808 nm laser irradiation. The <I>in vitro</I> experimental result shows that the PB@MSH-EDA NPs are biocompatible and could be efficiently taken up by MDA-MB-231 cells. In addition, the <I>in vivo</I> results demonstrate that the tumor-bearing mice fully recovered after injecting the drug (Dox)-loaded PB@MSH-EDA/Dox NPs and being further irradiated with the 808 nm laser. We believe that the PB@MSH-EDA NPs system could be utilized as an efficient PAI-guided chemo-photothermal therapy agent for the detection and treatment of tumors in an emerging cancer therapy application.</P>
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