시간 스케일러빌리티를 이용한 MPEG-4 FGS 비디오 부호화 기법

김종욱,이배호 전남대학교 전자통신기술연구소 2002 전자통신기술논문지 Vol.5 No.1

The paper shows MPEG-4 FGS(Fine Granularity Scalability) using the scalable video encoding technique that is adapted by MPEG-4 standard and a scheme to improve its efficiency. MPEG-4 FGS is the encoding technique that has the good flexibility for various property of devices and bandwidth variation over the internet. To improve MPEG-4 FGS coding efficiency, we applied an encoding technique with temporal scalability. In other words, the base layer is encoded with the same technique as the typical MPEG-4 FGS and the enhancement layer is encoded by adding temporal scalability property In test results, we could know that had better coding efficiency in the case of adding temporal scalability for the enhancement layer.

Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

Lee, Jong-Uk,Cheong, Hyun Sub,Shim, Eun-Young,Bae, Da-Jeong,Chang, Hun Soo,Uh, Soo-Taek,Kim, Young Hoon,Park, Jong-Sook,Lee, Bora,Shin, Hyoung Doo,Park, Choon-Sik BioMed Central 2017 Respiratory research Vol.18 No.-

<P><B>Background</B></P><P>Idiopathic pulmonary fibrosis (IPF) is characterized by the complex interaction of cells involved in chronic inflammation and fibrosis. Global gene expression of a homogenous cell population will identify novel candidate genes.</P><P><B>Methods</B></P><P>Gene expression of fibroblasts derived from lung tissues (8 IPF and 4 controls) was profiled, and ontology and functional pathway were analyzed in the genes exhibiting >2 absolute fold changes with <I>p</I>-values < 0.05. <I>CCL8</I> mRNA and protein levels were quantified using real-time PCR and ELISA. <I>CCL8</I> localization was evaluated by immunofluorescence staining.</P><P><B>Results</B></P><P>One hundred seventy eight genes differentially expressed and 15 genes exhibited >10-fold change. Among them, 13 were novel in relation with IPF. <I>CCL8</I> expression was 22.8-fold higher in IPF fibroblasts. The levels of <I>CCL8</I> mRNA and protein were 3 and 9-fold higher in 14 IPF fibroblasts than those in 10 control fibroblasts by real-time PCR and ELISA (<I>p</I> = 0.022 and <I>p</I> = 0.026, respectively). The <I>CCL8</I> concentrations in BAL fluid was significantly higher in 86 patients with IPF than those in 41 controls, and other interstitial lung diseases including non-specific interstitial pneumonia (<I>n</I> = 22), hypersensitivity pneumonitis (<I>n</I> = 20) and sarcoidosis (<I>n</I> = 19) (<I>p</I> < 0.005, respectively). Cut-off values of 2.29 pg/mL and 0.43 pg/mL possessed 80.2 and 70.7% accuracy for the discrimination of IPF from NC and the other lung diseases, respectively. IPF subjects with <I>CCL8</I> levels >28.61 pg/mL showed shorter survival compared to those with lower levels (<I>p</I> = 0.012). <I>CCL8</I> was expressed by α-SMA-positive cells in the interstitium of IPF.</P><P><B>Conclusions</B></P><P>Transcriptome analysis identified several novel IPF-related genes. Among them, <I>CCL8</I> is a candidate molecule for the differential diagnosis and prediction of survival.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12931-016-0493-6) contains supplementary material, which is available to authorized users.</P>

무선 애드 혹 기반 액티브네트워크상에서 QoS지원에 관한 연구

李基源,裵振勝,丁燦赫,金玄郁,李光培,姜景仁,朴京培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In this thesis, we propose a QoS supporting method on wireless ad hoc-based active network for improving reliability. On the loss of route path, the existing DSR routing protocol has several problems with wireless network communication because of decease of data reception rate and increase of network load. The proposed method improves reliability of data transfer by using functions of active network. That is, by using functions of active nodes and active packets, the proposed method supports QoS with reservation transfer method that saves data packets on transfer at intermediate nodes with lost path and then re-transmit the packets after route reconfiguration.

Prognostic impact of telomeric repeat-containing RNA expression on long-term oncologic outcomes in colorectal cancer

Bae, Sung Uk,Park, Won-Jin,Jeong, Woon Kyung,Baek, Seong Kyu,Lee, Hye-Won,Lee, Jae-Ho,Yang., Hua Williams & Wilkins Co 2019 Medicine Vol.98 No.14

<P><B>Abstract</B></P><P>Telomeres are transcribed into long, noncoding telomeric repeat-containing RNAs (TERRA) that have been implicated in the regulation of telomerase, the enzyme that lengthens telomeres, in heterochromatin formation at telomeres, and in telomere stability. This study aimed to evaluate the correlation between TERRA expression and long-term oncologic outcomes in colorectal cancer (CRC).</P><P>We evaluated 18p TERRA expression and telomere length using quantitative real-time PCR in 60 patients who underwent surgical resection for CRC between June 2008 and November 2010.</P><P>Patients were grouped according to 18p TERRA expression, with 29 (48.3%) and 31 (51.7%) patients in the low and high TERRA expression groups, respectively. The median follow-up period was 80 months (range 2–103). The 18p TERRA expression was marginally significantly associated with preoperative carcinoembryonic antigen (CEA; <I>P</I> = .082) and was significantly associated with telomere length (<I>P</I> < .05). Multivariate analysis revealed that preoperative CEA (hazard ratio [HR], 2.728; 95% confidence interval [CI], 0.832–8.944, <I>P</I> = .098) and 18p TERRA expression (HR, 0.113; 95% CI, 0.011–1.126, <I>P</I> = .071) were marginally significant independent prognostic factors for overall survival (OS), whereas preoperative CEA (HR, 4.254; 95% CI, 1.394–12.985, <I>P</I> = .011) and 18p TERRA expression (HR, 0.108; 95% CI, 0.011–1.037, <I>P</I> = .054) were significant independent prognostic factors for disease-free survival (DFS). According to our prognostic model with 2 prognostic factors, the OS and DFS rate increased to 76.2% and 80.63%, respectively, in patients with high 18p TERRA expression and CEA levels ≤5 (<I>P</I> = .178, <I>P</I> = .057, respectively).</P><P>18p TERRA expression was marginally significantly associated with preoperative CEA and significantly associated with telomere length, rendering it a potential prognostic factor for long-term oncologic outcomes in CRC.</P>

Exogenous γ-tocotrienol promotes preimplantation development and improves the quality of porcine embryos

Lee, Enok,Min, Sung-Hun,Song, Bong-Seok,Yeon, Ji-Yeong,Kim, Jin-Woo,Bae, Jung-Ho,Park, Soo-Yong,Lee, Yong-Hee,Kim, Sun-Uk,Lee, Dong-Seok,Chang, Kyu-Tae,Koo, Deog-Bon CSIRO Publishing 2015 Reproduction, fertility, and development Vol.27 No.3

<P> γ-tocotrienol (GTT), an isomer of vitamin E, has been the subject of increasing interest due to its strong anti-oxidant effects. Therefore, in this study, the effects of GTT on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in preimplantation porcine embryos. After in vitro maturation and fertilisation, porcine embryos were cultured for 6 days in porcine zygote medium 3 supplemented with or without GTT (200 μM) under oxidative stress conditions (200 μM hydrogen peroxide (H2O2)). Blastocyst development was significantly improved in the GTT-treated group when compared with the H2O2-treated group (P < 0.05). Subsequent evaluation of the intracellular levels of ROS and numbers of apoptotic nuclei in GTT-treated blastocysts revealed that ROS levels of GTT-treated porcine blastocysts were decreased (P < 0.05) and the numbers of apoptotic nuclei were reduced by GTT treatment in porcine embryos. Moreover, the total cell numbers of blastocysts were significantly increased in the GTT-treated group relative to the untreated group under H2O2-induced oxidative stress (P < 0.05). The expression levels of apoptosis-related genes (BCL-XL, BAX) in GTT-treated blastocysts were then investigated using real-time reverse transcription polymerase chain reaction. Expression of the anti-apoptotic BCL-XL gene was shown to be increased in the GTT-treated blastocyst group, whereas expression of the pro-apoptotic BAX gene was decreased. Taken together, these results suggest that GTT (200 μM) under H2O2-induced oxidative stress, thereby improving the developmental competence of porcine embryos via modulation of intracellular levels of ROS and the apoptotic index during the preimplantation stage. </P>

Alu-Derived Alternative Splicing Events Specific to Macaca Lineages in CTSF Gene

Lee, Ja-Rang,Park, Sang-Je,Kim, Young-Hyun,Choe, Se-Hee,Cho, Hyeon-Mu,Lee, Sang-Rae,Kim, Sun-Uk,Kim, Ji-Su,Sim, Bo-Woong,Song, Bong-Seok,Jeong, Kang-Jin,Lee, Youngjeon,Jin, Yeung Bae,Kang, Philyong,Hu Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.2

Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element-AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crabeating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues. Results demonstrated that AluYRa1 was only integrated into the genome of Macaca species and this lineage-specific integration led to exonization events by producing a conserved 3' splice site. Six transcript variants (V1-V6) were generated by alternative splicing (AS) events, including intron retention and alternative 5' splice sites in the 5' and 3' flanking regions of CTSF_AluYRa1. Among them, V3-V5 transcripts were ubiquitously expressed in all tissues of rhesus monkey and crab-eating monkey, whereas AluYRa1-exonized V1 was dominantly expressed in the testis of the crab-eating monkey, and V2 was only expressed in the testis of the two monkeys. These five transcript variants also had different amino acid sequences in the C-terminal region of CTSF, as compared to reference sequences. Thus, species-specific Alu-derived exonization by lineage-specific integration of Alu elements and AS events seems to have played an important role during primate evolution by producing transcript variants and gene diversification.

Identification of Alternative Variants and Insertion of the Novel Polymorphic <i> AluYl17</i> in <i> TSEN54</i> Gene during Primate Evolution

Lee, Ja-Rang,Kim, Young-Hyun,Park, Sang-Je,Choe, Se-Hee,Cho, Hyeon-Mu,Lee, Sang-Rae,Kim, Sun-Uk,Kim, Ji-Su,Sim, Bo-Woong,Song, Bong-Seok,Jeong, Kang-Jin,Lee, Youngjeon,Jin, Yeung Bae,Kang, Philyong,Hu Hindawi Publishing Corporation 2016 International journal of genomics Vol.2016 No.-

<P><I>TSEN54</I> encodes a subunit of the tRNA-splicing endonuclease complex, which catalyzes the identification and cleavage of introns from precursor tRNAs. Previously, we identified an<I> AluSx</I>-derived alternative transcript in<I> TSEN54</I> of cynomolgus monkey. Reverse transcription-polymerase chain reaction (RT-PCR) amplification and<I> TSEN54</I> sequence analysis of primate and human samples identified five novel alternative transcripts, including the<I> AluSx</I> exonized transcript. Additionally, we performed comparative expression analysis via RT-qPCR in various cynomolgus, rhesus monkey, and human tissues. RT-qPCR amplification revealed differential expression patterns. Furthermore, genomic PCR amplification and sequencing of primate and human DNA samples revealed that<I> AluSx</I> elements were integrated in human and all of the primate samples tested. Intriguingly, in langur genomic DNA, an additional<I> AluY</I> element was inserted into<I> AluSx</I> of intron eight of<I> TSEN54</I>. The new<I> AluY</I> element showed polymorphic insertion. Using standardized nomenclature for<I> Alu</I> repeats, the polymorphic<I> AluY</I> of the langur<I> TSEN54</I> was designated as being of the<I> AluYl17</I> subfamily. Our results suggest that integration of the<I> AluSx</I> element in<I> TSEN54</I> contributed to diversity in transcripts and induced lineage- or species-specific evolutionary events such as alternative splicing and polymorphic insertion during primate evolution.</P>

GPS를 이용한 퍼지형 자동주행 모델 개발

이종달(Lee Jong Dal),권기욱(Kwon Kee Uk),이영욱(Lee Young Uk),배건환(Bae Gun Hwan) 대한토목학회 2001 대한토목학회논문집 D Vol.21 No.6D

석분토를 이용한 지하공동 충전재 개발에 관한 연구

이상욱(Lee Sang-Uk),이대경(Lee Dae-Kyung),조성현(Cho Sung-Hyun),박상훈(Park Sang-hun),배기선(Bae Ki-Sun),마상준(Ma Sang-Joon) 한국구조물진단유지관리학회 2005 한국구조물진단학회 학술발표회논문집 Vol.- No.-

Underground cavities are classified into two large groups, lime cavities and dead mines. Fatal damages, ground depression and subside, caused by these cavities are increased. Also, stone dusts are plt out around O.8-1million ㎥ per year on quarrying process and cause serious pollution problems. In this study, the feasibility of an application reinforcement method was studied to develope the new economical and effective fillers by recycling the stone dusts which are treated as industrial wastes and do not have any economic value(valueless). Through the experiment a low unit cost underground cavity filler could be developed from stone dusts, the wastes, after producing mortar include over 90%(volume) stone dust solution, meeting physical and chemical features(total 13 types of mixture of gel type, underwater un-separation and other physical property tests according to the each features) correspond with environmental condition of underground cavity.

석분토를 이용한 지하공동 충전재 개발에 관한 연구

이상욱 ( Lee Sang-uk ),이대경 ( Lee Dae-kyung ),조성현 ( Cho Sung-hyun ),박상훈 ( Park Sang-hun ),배기선 ( Bae Ki-sun ),마상준 ( Ma Sang-joon ) 한국구조물진단유지관리공학회 2005 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.9 No.1

Underground cavities are classified into two large groups, lime cavities and dead mines. Fatal damages, ground depression and subside, caused by these cavities are increased Also, stone dusts are put out around 0.8 ~1million ㎥ per year on quarrying process and cause serious pollution problems. In this study, the feasibility of an application reinforcement method was studied to develope the new economical and effective fillers by recycling the stone dusts which are treated as industrial wastes and do not have any economic value(valueless). Through the experiment, a low unit cost underground cavity filler could be developed from stone dusts, the wastes, after producing mortar include over 90%(volume) stone dust solution, meeting physical and chemical features(total 13 types of mixture of gel type, underwater un-separation and other physical property tests according to the each features) correspond with environmental condition of underground cavity.