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Hyoung-Joon Moon,Seong-Jun Park,김혜권,Soo-Kyung Ann,Semi Rho,Hyun-Ok Keum,Bong-Kyun Park 대한수의학회 2010 Journal of Veterinary Science Vol.11 No.3
The purpose of this study was to develop a multiplex PCR that can detect porcine endogenous retrovirus (PERV)proviral genes (pol, envA, envB, envC) and porcine mitochondrial DNA, using a dual priming oligonucleotide (DPO) system. The primer specifically detected the PERV proviral genes pol, envA, envB, envC, and porcine mitochondrial DNA only in samples of pig origin. The sensitivity of the primer was demonstrated by simultaneous amplification of all 5 target genes in as little as 10 pg of pig DNA containing PERV proviral genes and mitochondrial DNA. The multiplex PCR, when applied to field samples,simultaneously and successfully amplified PERV proviral genes from liver, blood and hair root samples. Thus, the multiplex PCR developed in the current study using DPO-based primers is a rapid, sensitive and specific assay for the detection and subtyping of PERV proviral genes.
( Tae Hee Lee ),( Joon Seong Lee ),( Suyeon Park ),( Kyung-ann Lee ),( Hyun-sook Kim ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.6
Background/Aims: Systemic sclerosis (SSc) is associated with a wide range of gastrointestinal (GI) changes. The University of California-Los Angeles Scleroderma Clinical Trial Consortium Gastrointestinal Tract (UCLA SCTC GIT 2.0) instrument is a self-administered GI assessment instrument for patients with SSc. We developed a Korean version of the UCLA SCTC GIT 2.0 instrument and evaluated its reliability and internal consistency. Methods: The participants were 37 Korean patients with SSc. Translation and cross-cultural adaptation of the UCLA SCTC GIT 2.0 were performed according to international standardized guidelines. We evaluated reproducibility by calculating the intraclass correlation coefficients and assessed the internal consistency of the Korean version of the UCLA SCTC GIT 2.0. We assessed its construct validity by evaluating its correlations with the Short Form Health Survey version 2 and EQ-5D scores by means of Spearman correlation analyses. Results: Patients with SSc were mostly women (89.19%) with a mean age of 52.2 years, median disease duration of 24 months, and median modified Rodnan total skin score of 4. The median total GIT score on the UCLA SCTC GIT 2.0 was 0.3. The UCLA SCTC GIT 2.0 Korean version showed excellent internal consistency (Cronbach’s α of total GIT score = 0.863). Most domains of the ULCA SCTC GIT 2.0 were correlated with those of the EuroQol (EQ)-5D score. Conclusions: The Korean version of the UCLA SCTC GIT 2.0 has acceptable internal consistency, reliability, and validity. Therefore, it can be used to assess GIT involvement in Korean patients with SSc.
이서영,김성락,임종설,안성준,Lee Seo young,Kim Seong Rag,Lim Jong Seul,Ann Seong Joon The Korean Institute of Communications and Informa 2004 韓國通信學會論文誌 Vol.29 No.10A
이 논문은 페이딩 채널에서 직접확산 부호분할다중접속(DS-CDMA) 시스템을 위한 선형제약 변형 최소평균자 승오차(MMSE) 검출의 이전 연구에 대한 후속 연구이다. 변형된 MMSE 검출과 파일로트 심볼의 도움을 받는 채널추정(PSACE)의 결합시 발생하는 시스템 동작불능을 방지하는 조건을 구하였다. 선형제약 변형 MMSE의 해가 레일레이 페이딩에서의 시간변화에 대해 이론적으로 강건함을 보였다. 이 사실은 시뮬레이션 결과와 일치한다. 아울러 어떤 조건에서 선형제약 변형 MMSE 검출이 출력 신호대간섭-잡음비(SINR)를 최대화하였다. This paper follows up the previous work on the linearly constrained, modified minimum mean-squared error(MMSE) detection for direct-sequence code-division multiple-access DS-CDMA) systems in fading channels. We find a condition to avoid the breakdown of joint modified MMSE detection and pilot symbol-aided channel estimation (PSACE). The linearly constrained, modified MMSE solution is theoretically shown to be robust against time variations in Rayleigh fading channels. This fact is consistent with the simulation results. We also show that under some conditions the linearly constrained, modified MMSE detection maximizes the output signal-to-interference-plus-noise ratio.(SINR)