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Preemptive Analgesia with Acupuncture Monitored by c-Fos Expression in Rats
André T.A. Gonçalves de Freitas,Lino Lemonica,Julio De Faveri,Sergio Pereira,Maria D. Bedoya Henao 사단법인약침학회 2016 Journal of Acupuncture & Meridian Studies Vol.9 No.1
Pain behavior and awareness are characterized by heightened alertness and anxiety, which begin to disappear as soon as the curative process starts. The present study aimed to quantify c-fos expression in rat spinal cords and brains after a surgical stimulus and with preoperative or postoperative acupuncture. Animals were randomly divided into preoperative and postoperative groups and were then further divided into control, manual acupuncture (MA), or electroacupuncture (EA) groups. Expression of c-fos was quantified using immunohistochemistry. The collected data were analyzed using the t test at a 5% probability level. Presurgery and postsurgery spinal cord c-fos expressions were similar in all of the treatment groups. In the control rats, c-fos expression was higher before surgery than after surgery, contradicting the expected outcome of acupuncture and preemptive analgesia. After treatment, the expression of c-fos in the brains of the rats in the MA and the EA groups was reduced compared with that of the rats in the control group. These findings suggest that acupuncture used as preemptive analgesia in rats is a useful model for studying its application in human treatment.
Lateral pterygoid muscle volume and migraine in patients with temporomandibular disorders
Sérgio Lúcio Pereira de Castro Lopes,André Luiz Ferreira Costa,Thiago de Oliveira Gamba,Isadora Luana Flores,Adriana Dibo Cruz,Li Li Min 대한영상치의학회 2015 Imaging Science in Dentistry Vol.45 No.1
Purpose: Lateral pterygoid muscle (LPM) plays an important role in jaw movement and has been implicated in Temporomandibular disorders (TMDs). Migraine has been described as a common symptom in patients with TMDs and may be related to muscle hyperactivity. This study aimed to compare LPM volume in individuals with and without migraine, using segmentation of the LPM in magnetic resonance (MR) imaging of the TMJ. Materials and Methods: Twenty patients with migraine and 20 volunteers without migraine underwent a clinical examination of the TMJ, according to the Research Diagnostic Criteria for TMDs. MR imaging was performed and the LPM was segmented using the ITK-SNAP 1.4.1 software, which calculates the volume of each segmented structure in voxels per cubic millimeter. The chi-squared test and the Fisher’s exact test were used to relate the TMD variables obtained from the MR images and clinical examinations to the presence of migraine. Logistic binary regression was used to determine the importance of each factor for predicting the presence of a migraine headache. Results: Patients with TMDs and migraine tended to have hypertrophy of the LPM (58.7%). In addition, abnormal mandibular movements (61.2%) and disc displacement (70.0%) were found to be the most common signs in patients with TMDs and migraine. Conclusion: In patients with TMDs and simultaneous migraine, the LPM tends to be hypertrophic. LPM segmentation on MR imaging may be an alternative method to study this muscle in such patients because the hypertrophic LPM is not always palpable.
Gisela Andrade,André Pereira,Lucília Gonçalves,Cláudia Videira 한국유방암학회 2021 Journal of breast cancer Vol.24 No.4
Breast tissue markers are common in current clinical practice and are susceptible to migration. Herein, we present the case of a 47-year-old woman with invasive breast carcinoma diagnosed through ultrasound-guided core biopsy, who underwent placement of a breast marker (HydroMARK®) under ultrasound guidance 30 days after core biopsy and with subsequent marker migration to the nipple. The correct position of the marker was documented by mammography after its placement and by magnetic resonance imaging (MRI) after neoadjuvant chemotherapy. Migration of the marker to the nipple was evident only by mammography on the day of surgery. We hypothesized that an intraductal path was the route of marker migration in this patient. Marked ductal ectasia evident on MRI and histopathologic examination supported this hypothesis. To the best of our knowledge, this is the first published case of intraductal migration of a breast tissue marker.
Tiago de Souza Leite,Andréia Cnossen-Fassoni,Olinto Liparini Pereira,Eduardo Seiti Gomide Mizubuti,Elza Fernandes de Araújo,Marisa Vieira de Queiroz 한국미생물학회 2013 The journal of microbiology Vol.51 No.1
Fungal endophytes were isolated from the leaves of soybean cultivars in Brazil using two different isolation techniques – fragment plating and the innovative dilution-to-extinction culturing – to increase the species richness, frequency of isolates and diversity. A total of 241 morphospecies were obtained corresponding to 62 taxa that were identified by analysis of the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA). The Phylum Ascomycota predominated, representing 99% and 95.2% of isolates in the Monsoy and Conquista cultivars, respectively, whereas the Phylum Basidiomycota represented 1% and 4.8% of isolates, respectively. The genera Ampelomyces, Annulohypoxylon, Guignardia, Leptospora, Magnaporthe, Ophiognomonia, Paraconiothyrium, Phaeosphaeriopsis, Rhodotorula, Sporobolomyces, and Xylaria for the first time were isolated from soybean; this suggests that soybean harbours novel and highly diverse fungi. The yeasts genera Rhodotorula and Sporobolomyces (subphylum Pucciniomycotina) represent the Phylum Basidiomycota. The species richness was greater when both isolation techniques were used. The diversity of fungal endophytes was similar in both cultivars when the same isolation technique was used except for Hill’s index, N1. The use of ITS region sequences allowed the isolates to be grouped according to Order, Class and Phylum. Ampelomyces, Chaetomium, and Phoma glomerata are endophytic species that may play potential roles in the biological control of soybean pathogens. This study is one of the first to apply extinction-culturing to isolate fungal endophytes in plant leaves, thus contributing to the development and improvement of this technique for future studies.
Karine Rigon Zimmer,Carlos H. Blum-Silva,André Luiz Kulkamp Souza,Márcia WulffSchuch,Flávio Henrique Reginatto,Cláudio Martin P. Pereira,Alexandre José Macedo,Claiton Leonetti Lencina 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.3
The antibiofilm and antibacterial properties against Pseudomonas aeruginosa and Staphylococcus epidermidis and chemical characterization of six hydroethanolic blueberry extracts (blueberry rabbiteye—Vaccinium virgatum) from different cultivars and means of propagation were investigated. The total flavonoid, anthocyanin, and phenolic contents were determined by specific and well-established methods. Among the cultivars, Briteblue showed the lowest content of all metabolites analyzed, while Bluegem showed the highest concentrations of these compounds. All the micropropagated cultivars presented the highest amounts of chlorogenic acid. The blueberry fruit extracts showed strong activity against S. epidermidis biofilm (up to 84% inhibition) without inhibiting bacterial growth. Likewise, Bluegem micropropagated extract, which had the highest anthocyanin, flavonoids, and phenolic compound content, demonstrated the highest S. epidermidis biofilm inhibitory effect. Finally, a linear correlation between the total phenolic content and the percentage of biofilm inhibition was observed.
Queiroz Polyane Mazucatto,Fardim Karolina Castilho,Costa André Luiz Ferreira,Matheus Ricardo Alves,Lopes Sérgio Lúcio Pereira Castro 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.2
Purpose: The aim of this study was to evaluate changes in the trabecular bone through texture analysis and compare the texture analysis characteristics of different areas in patients with medication-related osteonecrosis of the jaw (MRONJ). Materials and Methods: Cone-beam computed tomographic images of 16 patients diagnosed with MRONJ were used. In sagittal images, 3 regions were chosen: active osteonecrosis (AO); intermediate tissue (IT), which presented a zone of apparently healthy tissue adjacent to the AO area; and healthy bone tissue (HT) (control area). Texture analysis was performed evaluating 7 parameters: secondary angular momentum, contrast, correlation, sum of squares, inverse moment of difference, sum of entropies, and entropy. Data were analyzed using the Kruskal-Wallis test with a significance level of 5%. Results: Comparing the areas of AO, IT, and HT, significant differences (P<0.05) were observed. The IT and AO area images showed higher values for parameters such as contrast, entropy, and secondary angular momentum than the HT area, indicating greater disorder in these tissues. Conclusion: Through texture analysis, changes in the bone pattern could be observed in areas of osteonecrosis. The texture analysis demonstrated that areas visually identified and classified as IT still had necrotic tissue, thereby increasing the accuracy of delimiting the real extension of MRONJ.
Costa Andre Luiz Ferreira,Fardim Karolina Aparecida Castilho,Ribeiro Isabela Teixeira,Jardini Maria Aparecida Neves,Braz-Silva Paulo Henrique,Orhan Kaan,de Castro Lopes Sérgio Lúcio Pereira 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.1
Purpose: This study aimed to assess texture analysis (TA) of cone-beam computed tomography (CBCT) images as a quantitative tool for the differential diagnosis of odontogenic and non-odontogenic maxillary sinusitis (OS and NOS, respectively). Materials and Methods: CBCT images of 40 patients diagnosed with OS (N = 20) and NOS (N = 20) were evaluated. The gray level co-occurrence (GLCM) matrix parameters, and gray level run length matrix texture (GLRLM) parameters were extracted using manually placed regions of interest on lesion images. Seven texture parameters were calculated using GLCM and 4 parameters using GLRLM. The Mann-Whitney test was used for comparisons between the groups, and the Levene test was performed to confirm the homogeneity of variance (α = 5%). Results: The results showed statistically significant differences (P<0.05) between the OS and NOS patients regarding 3 TA parameters. NOS patients presented higher values for contrast, while OS patients presented higher values for correlation and inverse difference moment. Greater textural homogeneity was observed in the OS patients than in the NOS patients, with statistically significant differences in standard deviations between the groups for correlation, sum of squares, sum of entropy, and entropy. Conclusion: TA enabled quantitative differentiation between OS and NOS on CBCT images by using the parameters of contrast, correlation, and inverse difference moment.
de Abreu, Andre LP,Gimenes, Fabricia,Malaguti, Natalia,Pereira, Monalisa W,Uchimura, Nelson S,Consolaro, Marcia EL Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.7
To determine the prevalence of human papillomavirus (HPV) among women with atypical squamous cells of undetermined significance (ASC-US) referred to colposcopy and the implications for clinical management in low- and middle-income countries (LMIC), the present study was conducted. We included 200 women living in $Maring{\acute{a}}$/Brazil referred to colposcopy service between August 2012 and March 2013 due to an abnormal cytology from ASC-US until high-grade intraepithelial lesion (HSIL). HPV was detected and genotyped by polymerase chain reaction (PCR). The mean age was $36.8{\pm}10.5$ years, and women with and without ASC-US had similar mean ages ($37.4{\pm}11.5$ and $36.4{\pm}9.96$ years, respectively). The highest prevalence of ASC-US occurred at 20-24 years (40%). HPV-DNA was positive in 164 (82.0%) women.Of the 57 women with ASC-US, 30 (52.6%) were HPV-DNA-positive and 21 (70%) were high-risk HPV-positive (HR-HPV); the latter was similar to women without ASC-US (76.9%) but with other abnormal cytological findings present. Our data demonstrated that performing tests for HR-HPV can be used for management of women with ASC-US to support the decision of which women should be referred for an immediate or later colposcopy. The same conclusions can be applied to other LMICs for which HPV testing for primary screening has not been adopted.
Lateral pterygoid muscle volume and migraine in patients with temporomandibular disorders
Lopes, Sergio Lucio Pereira De Castro,Costa, Andre Luiz Ferreira,Gamba, Thiago De Oliveira,Flores, Isadora Luana,Cruz, Adriana Dibo,Min, Li Li Korean Academy of Oral and Maxillofacial Radiology 2015 Imaging Science in Dentistry Vol.45 No.1
Purpose: Lateral pterygoid muscle (LPM) plays an important role in jaw movement and has been implicated in Temporomandibular disorders (TMDs). Migraine has been described as a common symptom in patients with TMDs and may be related to muscle hyperactivity. This study aimed to compare LPM volume in individuals with and without migraine, using segmentation of the LPM in magnetic resonance (MR) imaging of the TMJ. Materials and Methods: Twenty patients with migraine and 20 volunteers without migraine underwent a clinical examination of the TMJ, according to the Research Diagnostic Criteria for TMDs. MR imaging was performed and the LPM was segmented using the ITK-SNAP 1.4.1 software, which calculates the volume of each segmented structure in voxels per cubic millimeter. The chi-squared test and the Fisher's exact test were used to relate the TMD variables obtained from the MR images and clinical examinations to the presence of migraine. Logistic binary regression was used to determine the importance of each factor for predicting the presence of a migraine headache. Results: Patients with TMDs and migraine tended to have hypertrophy of the LPM (58.7%). In addition, abnormal mandibular movements (61.2%) and disc displacement (70.0%) were found to be the most common signs in patients with TMDs and migraine. Conclusion: In patients with TMDs and simultaneous migraine, the LPM tends to be hypertrophic. LPM segmentation on MR imaging may be an alternative method to study this muscle in such patients because the hypertrophic LPM is not always palpable.