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Phuong T. Ho(Phuong T. Ho ),Hee-Seong Byun(Hee-Seong Byun),Thuy T. B. Vo(Thuy T. B. Vo ),Aamir Lal(Aamir Lal ),Sukchan Lee(Sukchan Lee),Eui-Joon Kil(Eui-Joon Kil) 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.3
Sweet potato symptomless virus 1 (SPSMV-1) is a single-stranded circular DNA virus, belonging to the genus Mastrevirus (family Geminiviridae) that was first identified on sweet potato plants in South Korea in 2012. Although SPSMV-1 does not induce distinct symptoms in sweet potato plants, its co-infection with different sweet potato viruses is highly prevalent, and thus threatens sweet potato production in South Ko-rea. In this study, the complete genome sequence of a Korean isolate of SPSMV-1 was obtained by Sanger sequencing of polymerase chain reaction (PCR) am-plicons from sweet potato plants collected in the field (Suwon). An infectious clone of SPSMV-1 (1.1-mer) was constructed, cloned into the plant expression vec-tor pCAMBIA1303, and agro-inoculated into Nicotiana benthamiana using three Agrobacterium tumefaciens strains (GV3101, LBA4404, and EHA105). Although no visual differences were observed between the mock and infected groups, SPSMV-1 accumulation was detected in the roots, stems, and newly produced leaves through PCR. The A. tumefaciens strain LBA4404 was the most effective at transferring the SPSMV-1 genome to N. benthamiana. We confirmed the viral replication in N. benthamiana samples through strand-specific amplifi-cation using virion-sense- and complementary-sense-specific primer sets.
Seasonal dynamics of PPV accumulation in peach trees according to temperatures
Eui-Joon Kil,Zarghami D Samira,Chairina Fadhila,Vo Thi Bich Thuy,Aamir Lal,Ju Hee We,Ho Gyun Park,Yongjun Lee,Dongjun Kim,Hae-Ryun Kwak,Mikyeong Kim,Hong-Soo Choi,Sukchan Lee 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10
Newly emerging geminiviruses in Korea
Eui-Joon Kil,Chairina Fadhila,Vo Thi Bich Thuy,Aamir Lal,Samira Zarghami D.,Hee-Seong Byun,Chung-Kyoon Auh,Ji-Kwang Kim,Joong-Hwan Lee,Gwan-Seok Lee,Chang-Seok Kim,Hong-Soo Choi,Kyeong-Yeoll Lee,Sukch 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10
Geminiviruses are plant-infecting viruses with monopartite or bipartite single-stranded circular DNA genomes. They are known to be mediated by insects such as whiteflies, treehoppers, leafhoppers or aphids and can cause devastating plant diseases in a wide range of economically significant crops worldwide. In Korea, occurrence of geminiviruses were reported officially after the 2000s. Although Honeysuckle yellow vein virus (HYVV) and Sweet potato leaf curl virus (SPLCV) were identified from honeysuckle and sweet potato in 2004 and 2006 respectively, these viruses did not spread and cause much concern for geminiviruses. In 2008, Tomato yellow leaf curl virus (TYLCV) that has caused severe tomato production loss in many subtropical and tropical countries was first reported in tomato plants cultivated in Tongyeong. TYLCV rapidly spread through the country and has been continuously reported from tomato cultivating areas in Korea. In addition to TYLCV, Tobacco leaf curl virus and Sweet potato golden vein associated virus have occurred. In recent years, new geminiviruses including Papaya leaf curl Guangdong virus (PaLCGdV) and Euphorbia leaf curl virus (EuLCV) have been introduced. Newly emerging geminiviruses from tropical and subtropical countries are due to increased demand for various fruits and vegetables, and climate change. In addition, there are reports that some geminiviruses including TYLCV can be transmitted by infected seeds. Therefore it is important to study on epidemiology of virus introduction and spread among the countries and within country.