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정원줄기세포의 이종간 이식을 위한 효율적인 수여(Recipient) 생쥐 생산 기법 개발
김기중 ( Ki Jung Kim ),임한 ( Han Lim ),왕종현 ( Jong Hyun Wang ),김병각 ( Byung Gak Kim ),이용안 ( Yong An Lee ),김방진 ( Bang Jin Kim ),김용희 ( Yong Hee Kim ),홍영호 ( Yeong Ho Hong ),김근배 ( Geun Bae Kim ),류범용 ( Buom Yong 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.1s
Spermatogonial xenotransplantation assay provides access to the several species germline and has been used in experimental animal models to study stem cell biology and germline development. To make the xenotransplantation technology more extensively accessible, it is require development of recipient preparation protocols. Therefore, we propose an optimal method for preparation of athymic nude recipient with busulfan treatment. To investigate the optimal concentration of busulfan treatment, athymic nude mice (6~8 weeks old) were intraperitoneally injected with different concentrations of busulfan (0, 15, 25, 35 and 45 mg/kg). 6~8 weeks after busulfan treatment, testicular weight was significantly lower in 45 mg/kg treated group than the other groups. And the percentage of spermatogenic tubule was significantly reduced in 45 mg/kg of busulfan treated recipient testis. Also we compared the gene expression level of sertoli-cell-derived growth factor, glial-cell-line-derived neurotrophic factor (GDNF) as a potential measure of niche function. Relative to the other experimental groups, 45 mg/kg busulfan treated recipient testis showed higher GDNF expression. Next, to find out the appropriate time to transplantation after treated with busulfan (45 mg/kg), the rat testicular donor cells were transplanted into recipient testis at 2, 4, 6 and 8 weeks after treated with busulfan. Analysis of recipient mice revealed that transplantation of donor cells on 6 weeks after busulfan treatment was the most effective time to support high level of donor stem cell engraftment. In this study, we provide an optimum experimental condition for preparation of recipient mouse model system for xenotransplantation. Also, our data could contribute to recipient preparation in other species.
자동차용 Seat Motor Housing의 부품 일체화에 관한 연구
이동욱(Tong Uk Lee),김병각(Byung Gak Kim),김인관(In Kwan Kim),김영수(Young Soo Kim) 한국자동차공학회 2006 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
The general way of cost reduction in automobiles was focused on the substitute the material from combination to parts composites. The main research is focused on the substitutive design of Seat Motor Housing, with materials by the hot chamber diecasting process with zinc alloy diecasting. The newly developed parts were compared with the original products. The diecasting process was used to manufacture a Seat Motor Housing for automobile. Seat Motor Housing was modelled with UNI GRAPHICS that analyze with Z-CAST software. Z-CAST was computer software with FDM(finite difference method). The analysis results obtained about filling behavior and solidification of casting showed good agreement with test results. New product was evaluation of the performance. In result, Diecasting of Seat Motor Housing is expected to be best fitted in Works.
자동차용 시트 모터 하우징의 다이캐스팅 품질 향상에 관한 연구
이동욱(Tong-uk Lee),김병각(Byung-Gak Kim),김인관(In-Kwan Kim),김영수(Young-Soo Kim) 한국기계가공학회 2006 한국기계가공학회 춘추계학술대회 논문집 Vol.2006 No.-
In recent years, the automobile industry has focused on the cost reduction through integration of parts andproductivity increase by reducing the number of processes through modulation. Research on the integration of parts leads to quality improvement through integrated design of parts, and changes in material property and production process. This study presents a way to integrate seat motor housing parts, which comprise two caps and one housing in the existing products, into one part to reduce breakage during assembly. For the production of hot chamber die casting prototype, a process is designed using UNI GRAPHICS modeling. Z-CAST, a software utilizing finite difference method(FDM), is used to estimate defects and evaluate the prototype. The reliability test showed that the prototype satisfied material property, noise and vibration requirements. It also satisfied the required specification of run out test. As the prototype reduced the number of processes and cost, die casting is most appropriate for the production of seat motor housings.
설치류에서 정소조직의 체외배양을 통한 정자형성과정에 관한 연구
정미선 ( Mi Seon Jung ),김방진 ( Bang Jin Kim ),이용안 ( Yong An Lee ),김기중 ( Ki Jung Kim ),김용희 ( Yong Hee Kim ),강현구 ( Hyun Gu Kang ),정상은 ( Sang Eun Jung ),김병각 ( Byung Gak Kim ),류범용 ( Buom Yong Ryu ) 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.1s
Spermatogenesis is a complex developmental process of continuous cell division involving a phase of germ cell expansion, meiotic division and cytodifferentiation that ultimately generates mature gametes. The object of this study was the development of optimal culture system in order to induce spermatogenesis in vitro and for its establishment. We collected immature testicular tissues from different developmental-stage of mice and rats which were subsequently cultured on the agarose gel with serum-free culture media. After appropriate culture period, we counted multiple layered seminiferous tubules in the cultured testis tissue to find out whether any differentiation occured following spermatogenesis. Between our experimental mouse strains ICR and C57, as a result, we observed significant increased spermatogenic tubules in case of C57 mouse strain in neonatal stage. In our optimized culture condition, we detected the lectin PNA positive spermatid resulted from spermatogenesis in cultured testis tissue after 6 weeks of incubation. We also injected the lentiviral transduced rat spermatogonial stem cells (SSCs) into recipient testis to generate the transgenic gametes. The testicular fragments were then cultured based on our optimized culture condition. After culturing 8 weeks, we observed expression of GFP in differentiated germ cells including post meiotic germ cells, which suggested the induction of donor-derived spermatogenesis. Also, in order to optimize the in vitro culture system for rat testis, we have tested the additional effect of hormones and growth factors, but could not observe the significant increased effect of hormone and growth factors treated groups than the control. In conclusion, our in vitro tissue culture methods could be applicable for production of in vitro transgenic gametes in various kinds of mammalian species including domestic animal.