FLC-mediated flowering repression is positively regulated by sumoylation

Son, Ga Hyun,Park, Bong Soo,Song, Jong Tae,Seo, Hak Soo Oxford University Press 2014 Journal of experimental botany Vol.65 No.1

<P>Flowering locus C (FLC), a floral repressor, is a critical factor for the transition from the vegetative to the reproductive phase. Here, the mechanisms regulating the activity and stability of the FLC protein were investigated. Bimolecular fluorescence complementation and <I>in vitro</I> pull-down analyses showed that FLC interacts with the E3 small ubiquitin-like modifier (SUMO) ligase AtSIZ1, suggesting that AtSIZ1 is an E3 SUMO ligase for FLC. <I>In vitro</I> sumoylation assays showed that FLC is modified by SUMO in the presence of SUMO-activating enzyme E1 and conjugating enzyme E2, but its sumoylation is inhibited by AtSIZ1. In transgenic plants, inducible <I>AtSIZ1</I> overexpression led to an increase in the concentration of FLC and delayed the post-translational decay of FLC, indicating that AtSIZ1 stabilizes FLC through direct binding. Also, the flowering time in mutant <I>FLC</I> (K154R, a mutation of the sumoylation site)-overexpressing plants was comparable with that in the wild type, whereas flowering was considerably delayed in <I>FLC</I>-overexpressing plants, supporting the notion that sumoylation is an important mechanism for FLC function. The data indicate that the sumoylation of FLC is critical for its role in the control of flowering time and that AtSIZ1 positively regulates FLC-mediated floral suppression.</P>

Transgenic Cucumber Expressing Nit Gene

Hyun A Jang,Ga Min Lim,Hyun A Kim,Youn-il Park,Suk Yoon Kwon,Pil Son Choi 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

To produce abiotic stress resistant transgenic cucumber, the cotyledonary node explants of cucumber (c.v. Eunsung) were inoculated with A. tumefaciens strain EHA105 containing the binary vector (pPZP211) carrying Nit gene. The 491 explants inoculated with bacterium solution for 30 min were maintained on 50 mg/L paromomycin contained shoot induction (SI) medium for first 2 weeks and then subcultured on 100 mg/L paromomycin to obtain transgenic adventitious shoots for 4 x 14 days. So far, 5 plant were selected, and then acclimated in soil. Of them, 3 transgenic plants with Nit gene were confirmed by Southern blot analysis.

자궁경부에 발생한 T/Natural killer cell 림프종

손가현 ( Ga Hyun Son ),전순례 ( Soon Rye Jeon ),조예성 ( Ye Sung Cho ),정현주 ( Hyun Joo Jung ),조혜진 ( Hye Jin Cho ),조희영 ( Hee Young Cho ),기정혜 ( Jeong Hae Kie ),최은경 ( Eun Kyoung Choi ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12

Non-Hodgkin`s lymphoma presented as a malignancy of the uterine cervix is exceedingly rare disease and accounts for approximately only 0.12-0.6% of extranodal lymphomas. Most reported cases were B-cell lymphomas and there is no consensus on the management and prognosis of the disease because of its extreme rarity. Primary T/natural killer (NK)-cell lymphoma arising from the uterine cervix is scarcely encountered that, in the review of literature, only 1 case has been reported by Mhawech et al. We encountered a woman with T/NK-cell lymphoma involving the uterine cervix that initially presented with severe vaginal bleeding. Therefore, we report the case with a brief review of literature.

Diabetes mellitus following platinum-based chemotherapy in gynecologic cancer patients

손가현 ( Ga Hyun Son ),( Jae Wook Kim ),( Young Tae Kim ),( Jae Hoon Kim ),( Eun Ji Nam ),( Bo Sung Yoon ),( Sang Won Kim ),( Sung Hoon Kim ) 대한산부인과학회 2006 대한산부인과학회 학술대회 Vol.92 No.-

Maternal Lipids as predictive factors for Large for gestational age newborns in women with gestational diabetes mellitus

손가현 (Ga Hyun Son ),Soo Jin Lee,Dong Wook Kwak,Seung Chul Lim,Ja Young Kwon,Young Han Kim,Yong Won Park 대한산부인과학회 2008 대한산부인과학회 학술대회 Vol.94 No.-

Platinum 제제가 포함된 항암화학요법을 받은 부인암 환자에서의 당뇨병 발생 위험

손가현 ( Ga Hyun Son ),남은지 ( Eun Ji Nam ),김상운 ( Sang Wun Kim ),김재훈 ( Jae Hoon Kim ),김영태 ( Young Tae Kim ),김성훈 ( Sung Hoon Kim ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.2

목적: 본 연구는 platinum 제제로 항암화학요법을 받은 부인암 환자에서 당뇨병 발생 위험과의 관계를 알아보고자 시행되었다. 연구 대상 및 방법: 2002년 1월부터 2005년 12월까지 세브란스병원 산부인과에서 항암화학요법을 받은 449명의 환자들 중 치료기간 중 공복혈장 포도당농도를 정기적으로 측정한 169명의 환자를 대상으로 하였으며 이 중 이미 당뇨병이 있는 22명을 제외하였다. 당뇨병이 발생한 8명의 환자를 대상으로 나이, 과거력 (흡연력, 음주력, 기타 내외과적 병력), 가족력, 신체 질량 지수, 혈장 포도당 농도, 암 종류, 항암제의 종류 및 용량, 항암치료받은 기간, 항암화학치료 후 당뇨병 발생시까지의 시간 등을 후향적으로 분석하였다. 결과: 항암화학요법 시행받은 환자들 중 8예 (4.8%)에서 당뇨병이 발생하였으며 환자의 평균 연령은 57세, 평균 신체질량지수는 27.0kg/m2였다. 모든 예에서 platinum 제제로 항암화학요법을 시행받았고 이 중 7예에서는 cisplatin 복합 또는 단독 항암화학요법 사용하였고 2예에서 carboplatin 제제를 사용하였다. 당뇨병 발생시까지 platinum 기반 항암제 누적 용량은 cisplatin의 경우 평균 252 mg/m2였고 carboplatin을 사용한 경우는 812 mg/m2였다. 항암화학요법 시행 후 평균 당뇨병 발생시기는 7개월이었다. 당뇨병이 발생한 환자 중 7명에서 당뇨병 치료로 경구 혈당강하제가 필요하였으며 1명에서는 식이요법만으로 혈당조절이 가능하였다. 본 연구 대상군에서의 당뇨병 발생률은 4.8%로 국내 60세 이상 노인인구에서의 당뇨병 발생률 2.1%보다 높은 경향을 보였다. 결론: 시스플라틴 등의 platinum 항암제를 사용하였을 경우 당뇨병 발생 위험을 고려하여 혈당을 정기적이고 주의깊게 측정하여야 하고 일반적으로 항암제 투여전 임상병리 검사 (pre-chemo lab values)에 포함되지 않는 혈당치 측정을 포함시키는 것을 고려하여야 한다. Objective: To assess the association between platinum-based chemotherapy for gynecologic malignancy and the risk of diabetes mellitus. Material and Methods: We analyzed retrospectively the association between platinum-based chemotherapy and diabetes mellitus. Out of the 449 patients who received the chemotherapy in Severance Hospital from January 2002 to December 2005, 169 patients with serial measurements of fasting blood glucose throughout the chemotherapy period were enrolled in this study. The parameters that were analyzed included age, past history, family history, body mass index (BMI), serum glucose, type of cancer, chemotherapy regimen, dose cycle, time after cycle. We performed binomial test to compare the incidence in our patients with that of general population. Results: In 8 patients (4.8%) diabetes mellitus developed during the treatment period. The median age of patients was 57 years, and the mean BMI was 27.0 kg/m2. All patients received platinum-based chemotherapy and seven of them received cisplatin based regimen and two patient was given carboplatin based regimen. Median cisplatin cumulative dose up until the diabetes mellitus occurred was 252 mg/m2 and that of carboplatin was 812 mg/m2. Median time until the diagnosis of diabetes mellitus after administration of the first chemotherapy cycle was 7 months. The overall incidence of hyperglycemia (4.8%) in patients treated with platinum-based chemotherapy is higher than the incidence of diabetes mellitus in the general population (2.1%). Conclusion: We suggest that regular monitoring of serum glucose levels which is not generally included in the pre-chemo lab values in patients receiving chemotherapy with platinum based regimen should be considered.

Evaluation of coronary artery atherosclerosis using CACS in asymptomatic postmenopausal women on hormone therapy

손가현 ( Ga Hyun Son ),( Da Jung Chung ),( Ja Seoung Koo ),( Ki Hyun Park ),( Byung Seok Lee ),( Dong Jae Cho ) 대한산부인과학회 2006 대한산부인과학회 학술대회 Vol.92 No.-

Comparison of the Outcome of History-indicated Cerclage and Transabdominal Cerclage in Twin Pregnancy

( Heejin Ham ),( Ga-hyun Son ),( Keun-young Lee ),( Ji Eun Song ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-

Objective: The purpose of this study was to compare the pregnancy outcomes of history-indicated cerclage (HIC) and transabdominal cerclage (TAC) in twin pregnancy. Methods: Retrospective chart review of twin pregnancies with HIC or TAC was performed. The study population underwent cerclage at Hallym University Kangnam Sacred Heart Hospital from 2007 to 2018. We compared the pregnancy outcomes between HIC and TAC group; The cerclage-to-delivery interval, the mean gestational age (GA) at cerclage, the mean GA at delivery, and birth weight were analyzed; The incidence of preterm birth (PTB) less than 28 weeks and 34 weeks, neonatal mortality were also compared between two groups. Results: Fifty-two patients were included; 19 patients underwent HIC, and 32 underwent TAC. There was no difference in the mean GA at cerclage between two groups (HIC, 13.73 weeks vs. TAC, 13.83 weeks, p=0.574). The mean GA at delivery in HIC group was 31.77 weeks, and that in TAC group was 35.85 weeks (p=0.003). There was statistically significant difference in the mean cerclage-to-delivery interval between two groups (HIC, 131.21days vs. TAC, 154days, p= 0.032) There were significant difference in neonatal birthweight between two groups (1st baby, 1846.84g vs. 2417.27g (p=0.004); 2nd baby 1732.11g vs. 2353.33, (p=0.001)), and neonatal mortality (HIC 4/19(21.1%) vs. TAC 1/33(3.0%), p=0.054). TAC group showed lower incidence of PTB < 28 weeks compared to HIC group (HIC 4/19 (21.1%) vs. TAC 1/33(3.0%), p=0.054). There was statistical difference in the incidence of PTB < 34weeks (HIC group, 8/19(42.1 %) vs. TAC group, 2/33(6.1 %), p=0.003). Conclusion: Both HIC and TAC were beneficial in prolonging pregnancy in twin pregnancy. TAC in twin pregnancy showed more favorable obstetric outcomes when compared to HIC.

Physical examination indicated cerclage in twin pregnancy : 11 years experiences in Hallym university medical center

( Suyeon Park ),( Ga-hyun Son ),( Keun-young Lee ),( Ji Eun Song ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-

Objective: To assess the outcome of physical exam indicated cerclage (PEIC) in twin pregnancy with bulging fetal membranes. Methods: This was a retrospective cohort study of 39 twin gestations at Hallym University Kangnam Sacred Heart Hospital from 2007 to 2018. The following variables were collected by chart review ; parity, gestational age at the time of cerclage, preoperative membranes bulging size, postoperative cervical length, GA at delivery, neonatal outcomes such as birth weight, neonatal survival. The overall results of PEIC in twin pregnancy were analyzed. Then subgroup analysis was performed according to the size of bulging fetal membranes (30 mm) to compare the outcomes between two groups. Results: Total 39 patients were identified, but 7 patients were excluded because of follow-up loss. Overall, 32 patients were analyzed. The mean size of bulging fetal membranes was 31.72mm and postoperative CL was 13.55mm. The average gestational age at PEIC was 22.01 weeks and the average GA at delivery was 27.76 weeks. The mean length of hospital stay was 24.72 days and the mean cerclage-to-delivery interval day was 40.28days. The perinatal survival rate was 81.25%(26/32). We analyzed subgroups according to the size of bulging fetal membrane (30mm) : 19 patients had bulging membrane 30mm (A group) and 13 patients had bulging membrane >30mm (B group). There were no statistically differences in postoperative cervical length, GA at PEIC GA at delivery, and cerclage-to-delivery interval between two group. There was no difference in birth weight, and fetal survival rate between two groups. Conclusion: PEIC in twin pregnancy showed favorable obstetric outcomes. It might be beneficial to perform PEIC in twin pregnancy with bulging fetal membranes to prolong gestational weeks. However, the size of bulging fetal membranes did not affect the pregnancy outcome of PEIC in twin gestation. Further study is needed to find appropriate prognostic factors of PEIC in twin pregnancy.

Protein disulfide isomerase has a regulatory role in endosperm development in rice

Yeon Jeong Kim,Ga Hyun Son,Markkandan Kesavan,Sung-Il Kim,Hak Soo Seo 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Protein disulfide isomerase (PDI) is a chaperone protein that involves in oxidative protein folding by acting as catalysts and folding assistants in the endoplasmic reticulum (ER). Genome database showed that rice contains three PDI-like genes. But, their functions and subcellullar localization are not clearly identified. Here, we show possible functions of rice PDI (OsPDI) during seed development. Seeds of OsPDI T-DNA insertion mutants which were identified by genomic DNA PCR and western blot display chalky phenotype. Electron microscope analysis revealed that endosperms of the OsPDIL1-1Δ mutant show imperfect packing of round starch granules, causing floury-white color. Abnormal form of protein body I (PB-I) containing prolamin and thick aleurone layer were also observed in the OsPDIL1-1Δ mutants. Protein content per seed was significantly low in the OsPDIL1-1Δ mutant. However, free sugar content was high in the OsPDIL1-1Δ mutant seed. Northern and western blot analyses showed that during seed development, OsPDI protein is steadily accumulated in the seed until maturation while its transcript level was highest at 10 days after flowering and rapidly decreased to basal level. In addition, OsPDI strongly interacts with cysteine protease OsCP1 and chaperone BiP protein accumulates in OsPDIL1-1Δ mutant. Besides, proteomic analysis of the OsPDIL1-1Δ mutant seed showed that OsPDI is post-translationally regulated and its loss causes accumulation of many types of seed proteins. Our results indicate that OsPDI plays a critical role in seed development through its regulatory activity for various proteins.