흰쥐에서 모린이 니페디핀의 약물동태에 미치는 영향

이종기,최준식,Lee, Chong-Ki,Choi, Jun-Shik 대한약학회 2007 약학회지 Vol.51 No.3

The aim of this study was to investigate the effect of morin on the pharmacokinetics of nifedipine in rats. The pharmacokinetic parameters of nifedipine were measured after the oral administration of nifedipine (5 mg/kg) in the presence or absence of morin (1.5, 7.5 and 15 mg/kg, respectively). Compared to the control groups, the presence of 7.5 mg/kg and 15 mg/kg of morin significantly (p<0.05) increased the area under the plasma concentration-time curve (AUC) of nifedipine by 48.5${\sim}$68.2%, and the peak concentration (C$_{max}$,) of nifedipine by 59.9~84.2%. The absolute bioavailability(AB%) of nifedipine was significantly (p<0.05) increased by 21.5${\sim}$24.5% compared to the control (14.5%). While there was no significant change in the time to reach the peak plasma concentration (T$_{max}$) and the terminal half-life (T$_{1/2}$) of nifedipine in the presence of morin. It might be suggested that morin altered disposition of nifedipine by inhibition of both the first-pass metabolism and p-glycoprotein (P-gp) efflux pump in the small intestine of rats. In conclusion, the presence of morin significantly enhanced the oral bioavailability of nifedipine, suggesting that concurrent use of morin or morin-containing dietary supplement with nifedipine should require close monitoring for potential drug interaction.

시판 노르플록사신 정계의 생체내 이용률

이종기,조삼상,Lee, Chong Ki,Cho, Sam Sang 한국임상약학회 1996 한국임상약학회지 Vol.6 No.2

This study was attempted to investigate the dissolution rate and the bioavailability after oral administration of commercially available norfloxacin tablets in rabbits. The dissolution test was conducted in artificial gastric juice using basket method with for norfloxacin preparations (A, B, C and D) which were chemically equivalent. The results were as follows ; The dissolution rate was increased in the order of four different brand A>D>B>C. Area under the plasma concentration curve and peak plasma concentration were increased in the order of brand A>D>B>C. Absorption rate constant and peak time were increased in the order of brand B>A>C>D, and there was a little difference in elimination rate constant and biological half-life. The correlation of the dissolution rate and relative bioavailability showed significant linear relationship. From the results of this experiment, the bioavailability of norfloxacin tablets in rabbits may be predicted from the results of dissolution rate studies.

흰쥐에 경구 및 정맥투여시 에피가로카테친이 니모디핀의 생체이용률에 미치는 영향

이종기,최준식,최동현,Lee, Chong-Ki,Choi, Jun-Shik,Choi, Dong-Hyun 대한약학회 2011 약학회지 Vol.55 No.4

The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on the pharmacokinetics of nimodipine in rats. Pharmacokinetic parameters of nimodipine were determined in rats after oral and iv administration of nimodipine with or without EGCG and also the effect of EGCG on the cytochrome P450 (CYP) 3A4 and P-glycoprotein (P-gp) activity were evaluated. EGCG inhibited CYP3A4 and P-gp activity. EGCG significantly increased the area under the plasma concentration-time curve (AUC) and peak plasma concentration ($C_{max}$) of nimodipine. The absolute bioavailability (AB%) and relative bioavailability (RB%) of nimodipine by EGCG were increased by 16% and by 48%, respectively, compared to the control. In contrast, EGCG did not affect the intravenous pharmacokinetics of nimodipine. Based on these results, the increased bioavailability of nimodipine might be due to inhibition of CYP3A4 in the small intestine and/or in the liver and inhibition of P-gp in the small intestine by EGCG.

숙지황 제조과정에 따른 성분함량 변화

이종기(Chong-Ki Lee),서정미(Jung-Mi Seo) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.10

숙지황은 제조방법에 따라 각각 그 제조과정의 기준을 달리하고 있어 건지황을 증숙하는 횟수에 따라 5-HMF와 당류의 함량변화를 분석하여 어느 증숙단계가 바람직한지를 검토하고 그 외에 총질소, 조지방, 회분 등을 측정하였다. 건지황에서는 5-HMF가 나타나지 않았고, 숙지황에서 증수(蒸數)에 따라 1증에서 6증까지는 5-HMF의 함량이 서서히 증가하다가 7증에서 크게 증가하였다. 당류는 건지황에서 이당류인 sucrose와 단당류인 fructose 및 glucose가 함유되었으나 증숙과정을 거친 후(1증~9증) sucrose가 나타나지 않았고, fructose와 glucose의 함량이 크게 증가하였다. 총질소는 건지황과 증숙과정을 거친 숙지황에서 큰 변화가 없었으며, 조지방의 경우 증숙과정을 거치는 동안 서서히 감소하였다. 회분은 대한약전(제8개정)의 기준(6.0% 이하)에 적합하였다. 숙지황의 품질관리를 위한 지표로서 5-HMF 함량이 대한약전(제8개정)의 기준(0.1% 이상)에 적합한 증숙과정은 본 연구에서는 7증 이상임을 알 수 있었다. 또한, 숙지황은 그 제조방법에 따라 그 성상 및 성분의 함량이 변화하므로 제조기준으로서 증숙단계를 어느 한 단계로 규정하기 보다는 지표성분인 5-HMF가 0.1% 이상인 제조과정이 어느 단계에 해당되는 것인지를 파악하여 제품을 생산하는 것이 바람직하다고 사료된다. This study was performed to obtain the good processing in the Rehmanniae Radix Preparata. The contents of the Rehmanniae Radix and the Rehmanniae Radix Preparata produced through different processes were analyzed in the 5-hydroxymethyl-2-furaldehyde (5-HMF), sugar, total nitrogen, crude lipid and ash. 5-HMF was not detected in the Rehmanniae Radix, but detected in the Rehmanniae Radix Preparata. 5-HMF content was increased gradually with processing times (1~9 times) and increased expressly in the Rehmanniae Radix Preparata steamed for 7 times. Sucrose, fructose and glucose were contained in the Rehmanniae Radix, but sucrose was not detected and fructose and glucose were increased largely in the Rehmanniae Radix Preparata steamed for 1 time. Fructose and glucose were decreased gradually with processing times (2~9 times), but the gap of decrease was insignificant. Total nitrogen was changed slightly and crude lipid was decreased slowly with processing times. The ash was suitable to KPⅧ rules (less than 6.0%). From this analysis we found out the content of 5-HMF from Rehmanniae Radix Preparata steamed more than 7 times was suitable to KPⅧ rules (more than 0.1%).

Oscilloscope 표시에 의한 廻轉體의 動 Balance

이종기(Chong Ki Lee) 대한전기학회 1971 전기의 세계 Vol.20 No.1

노르플록사신의 체내동태에 대한 신장해의 영향

이종기 ( Chong Ki Lee ) 한국약제학회 1995 Journal of Pharmaceutical Investigation Vol.25 No.3

The pharmacokinetics of norfloxacin (100㎎/㎏, oral) in renal failure rabbits was studied. Renal failure rabbits were induced by the i.v. injection of folate (50,100 and 150 ㎎/㎏,). These produced significant increases of serum creatinine concentration (S_(cr)) and blood urea nitrogen (BUN). Plasma concentration and AUC of norfloxacin significantly increased. Elimination rate constant (K_(el)) of norfloxacin significantly decreased, and half-life (t_½) of norfloxacin significantly increased. Correlation between serum creatinine concentration (S_(cr)) and half-life (t_(1/2)) of norfloxacin. and correlation between. BUN and AUC of norfloxacin have linear relationship respectively. These results suggest that adjustment of the dosage regimen of norfloxacin is desirable, and serum creatinine concentration (S_(cr)) as well as BUN can be used an index for adjusting the dosage regimen of norfloxacin in renal failure.

황금탕 및 황금추출물의 식중독 세균들에 대한 생육억제 효과

이종기(Chong Ki Lee),서진종(Jin Jong Seo) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.10

각종 식중독의 원인균으로 중요시 되는 11종의 균주에 대해서 황금탕 및 황금 추출물의 항균력을 검토한 결과, 황금탕 추출물은 Staphylococcus aureus, Bacillus cereus, Shigella sonnei, Shigella flexneri, Salmonella Typhimurium, Salmonella Enteritidis, Vibrio parahaemolyticus, Escherichia coli O111 및 Escherichia coli O126에서 항균력을 나타내었고, 황금 추출물은 Staphylococcus aureus, Bacillus cereus, Shigella sonnei, Shigella flexneri, Salmonella Typhimurium, Salmonella Enteritidis, Vibrio parahaemolyticus, Escherichia coli O111, Escherichia coli O126 및 Escherichia coli O157에서 항균력을 나타내었다. Listeria monocytogenes에 대해서는 황금탕 및 황금 추출물 모두 항균력이 없었고, Escherichia coli O157에 대해서는 황금탕 추출물은 항균력이 없었으나, 황금 추출물은 항균력을 나타내었다. 황금탕 추출물의 최소저해농도는 Staphylococcus aureus와 Bacillus cereus에서 40 ㎎/mL, Shigella flexneri와 Salmonella Enteritidis에서 100 ㎎/mL이었고, 황금 추출물의 최소저해농도는 Bacillus cereus에서 10 ㎎/mL, Staphylococcus aureus, Shigella flexneri 및 Salmonella Enteritidis에서 20 ㎎/mL이었으며, 황금탕 및 황금 추출물 모두 Staphylococcus aureus와 Bacillus cereus에 대해 48시간까지 배양하는 동안 최소저해농도 이상에서는 균이 거의 생육하지 못하였다. 이상에서 황금탕 추출물보다는 황금 추출물의 항균력이 더 강하며, 황금탕 추출물의 식중독 세균들에 대한 항균력은 황금에 의한 것으로 생각된다. 또한, 항균력이 확인된 세균으로 인한 각종 식중독에 황금탕의 사용가능성을 고려할 만하며, 황금 추출물에 대한 천연 식품 보존료로서의 이용 가능성을 검토할 가치가 있다고 사료된다. The effects of Whangkumtang extract and Scu1tellariae radix extract on the microbial growth were investigated. The antimicrobial activities and cell growth inhibiting effects were investigated to selected strains with different concentrations of Whangkumtang and Scutellariae radix extracts. Whangkumtang and Scutellariae radix extracts showed the antimicrobial activities on Staphylococcus aureus, Bacillus cereus, Shigella sonnei, Shigella flexneri, Salmonella Typhimurium, Salmonella Enteritidis, Vibrio parahaemolyticus, Escherichia coli O111 and Escherichia coli O126. Whangkumtang and Scutellariae radix extracts did not show the antimicrobial activity on Listeria monocytogenes. Scutellariae radix extract showed the antimicrobial activity on Escherichia coli O157 but Whangkumtang extract did not. Minimum inhibitory concentrations (MIC) of Whangkumtang extract for each strain appeared to 40 ㎎/mL on Staphylococcus aureus and Bacillus cereus, 100 ㎎/mL on Shigella Flexneri and Salmonella enteritidis. The MICs of Scutellariae radix extract appeared to 10 ㎎/mL on Bacillus cereus, 20 ㎎/mL on Staphylococcus aureus, Shigella flexneri and Salmonella Enteritidis. Scutellariae radix extract showed the higher antimicrobial activity than Whangkumtang extract. The cell growth inhibitions by Whangkumtang and Scutellariae radix extracts were observed from Staphylococcus aureus and Bacillus cereus, repectively, during 48-hr incubation period.

시메티딘과 이소니아짓의 약물 상호작용

이종기(Chong Ki Lee),이진환(Jin Hwan Lee),최준식(Jun Sick Choi) 대한약학회 1988 약학회지 Vol.32 No.5

Pharmacokinetic interaction of cimetidine and isoniazid was investigated in the rabbits. Isoniazid was administered orally at a dose of 30mg/kg to six rabbits after 10, 20, and 30mg/kg pretreatment of cimetidine twice a day for 10days. Concentration of the free and the total isoniazid in the blood and the urine was determined by spectrophotometer. Relative bioavailability and biological half-life(t1/2beta) were increased significantly by cimetidine pretreatment. Overall elimination rate constant and total clearance of isoniazid were decreased significantly by cimetidine pretreatment. The ratio of metabolites to isoniazid in the blood and the urine was decreased significantly by cimetidine pretreatment. Relative bioavailability, INAH to metabolites ratio in the blood and decrease in total clearance were highly correlated with the does of cimetidine pretreated. This result might be due to the inhibition of isoniazid metabolism in the liver by cimetidine pretreatment.

인진호 추출물의 식중독 세균들에 대한 생육억제 효과

이종기(Chong-Ki Lee),서진종(Jin-Jong Seo) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.8

천연 보존료의 개발을 위한 식품학적 기초자료로 활용하기 위해 인진호 추출물을 이용하여 9종의 병원균과 식중독균 Salmonella typhimurium에서는 80 mg/mL로 나타났으며, 측정된 48시간 동안까지 최소저해농도 이상에서는 균이 거의 생육하지 못하였다. 인진호 아세톤 추출물의 순차 분획물의 항균활성은 에틸아세테이트와 부탄올 분획물이 Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium, Vibrio parahaemolyticus에서, 클로로포름 분획물은 Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium에서 항균활성을 나내었으며, 헥산 분획물은 Bacillus subtilis에서만 항균활성을 나타내었다. 이같은 사실은 인진호 추출물의 항균성 물질은 특정 용매에만 용해되지 않고 다른 용매에도 용해되는 성분으로 단일 물질보다는 여러 성분일 것으로 생각된다. 이상의 결과에서 천연물에 따라 항균력을 가지는 물질이 다양하여 추출용매의 선정이 항균성을 나타내는 성분을 찾아내는데 중요하다고 생각되며, 또한 인진호에서 추출된 항균성 물질이 Staphylococcus aureus나 Vibrio parahaemolyticus 등 식중독균의 생육억제에 효과가 있을 것으로 사료된다. The solvent extracts of the aerial part of Artemisia capillaris extracted by using several solvents with different polarities were prepared and their antimicrobial activities were examined. The antimicrobial activities and cell growth inhibitions were investigated to each strain with the different concentrations of the aerial part of Artemisia capillaris. Acetone extract showed the highest antimicrobial activity. Minimum inhibitory concentrations (MIC) for each strain were appeared to 20 mg/mL at Staphylococcus aureus and Bacillus subtilis, 40 mg/mL at Vibrio parahaemolyticus, and 80 mg/mL at Salmonella typhimurium. The cell growth inhibitions were shown on Staphylococcus aureus, Bacillus subtilis, Vibrio parahaemolyticus, and Salmonella typhimurium for 48 hours. The acetone extract was further fractionated sequentially with hexane, chloroform, ethyl acetate, and butanol for purifying crude acetone extract. The solvent fraction of hexane, chloroform, ethyl acetate, and butanol showed the different antimicrobial activity, respectively.

濟州道 草地開發에 있어서 土壤學的 問題點

李鍾基(Chong Ki Lee),李根常(Keun Sang Lee) 한국토양비료학회 1975 한국토양비료학회지 Vol.8 No.3