Coulter Counter Model S-plus를 이용한 한국 성인의 혈소판수, 평균 혈소판용적, 혈소판 용적비 및 혈소판 분포폭의 정상치

咸健柱,金箕洪 한양대학교 의과대학 1981 한양의대 학술지 Vol.1 No.1

Gas Chromatography를 이용한 세균동정법의 임상적 유용성에 관한 연구

함건주,이정녀 인제대학교 1993 仁濟醫學 Vol.14 No.2

본 연구의 목적은 세균동정시 병원성이라고 알려진 세균만을 주로 동정하고 그 이외의 비병원성 혹은 상재균에 속하는 균종들은 무시되어 기회감염의 원인균인지 정확히 알 수가 없었기 때문에 세균세포벽에 존재하는 지방산을 이용한 동정법을 이용하여 흔히 볼 수 없는 세균들을 검출할 수 있는 가능성을 제시하여 임상에 도움을 주는 데 그 목적이 있다. Medically important microorganisms can be identified in many ways. Conventional methods usually rely on the enzyme activity. Immunodiagnostic and nucleotide hybridization techniques have improved sensitivity, specificity, precision, but required probe and high technique. Cellular fatty acid analysis which is one of the chemotaxonomy method is also precise and can result in the definition of high discrimmatory properties. The prospects for using GLC of bacterial cellular fatty acids in diagnostic microbiology have been appreciated for several years in our laboratory. Whole cell fatty acid methylester(FAME) profiles of the bacteria were studied using GLC with application of the fused silica capillary column and the results were as follows : 1.Staphylococcus aureus and coagulase-negative species have qualitatively similar FAME composition, but the quantitative differencies were distinguished from each species. They consist mainly of C14 : 0 iso, C15 : 0 iso, C15 :0 anteiso, C16 : 0, C18 :0, C20 :0. 2.Comparison of FAME of 3 different genera in Enterobacteriaceae which is Proteus milabilis, Klebsiella pneumonia, Citobacter diversus were very different. Typical FAME are C16 : 0, C17 : 0 cyclo, C19 : 0 cyclo, C16 : 1 cis 9, Most also contain C12 : 0 20H, C14 : 0 20H, Cl6 : 1 C in variable amounts. 3.Comparison of FAME of 4 different Family which is Bacillus thuringiensis, Acinetobacter baumannii, Aeromonas caviae, Salmonella choleraesuis were very different and could be distinguished from each other. In conclusion, this study provided clinical feasibility of utilizing GLC in rapid detection and Identification of several bacterial groups or species.

최근 7년간 부산 백병원의 혈액 배양에 대한 분석 결과(1980년-1986년)

함건주,홍숙희 인제대학교 1988 仁濟醫學 Vol.9 No.3

지난 7년간의 혈액 배양 결과를 검토한 결과 평균 양성분리율은 14.7%였으며 2회이상 의뢰된 경우 분리율이 높았다. 또한 연도별 분리율은 salmonella group이 감소되는 경향을, 장내 세균과 staphylococcus aureus 증가하는 경향을 보였으며 다른 지역과는 달리 salmonella group중 group A의 발견율이 높았다. 항생제 감수성 결과는 일부항생제에 저항성이 증가하는 것을 발견하였다. Total 13,037 blood culture specimens from 9,275 patients were cultured at InJe University Pusan Paik Hospital during the past 7 years(1980-1896). Blood culture results were analyzed and the following data were obtained. 1.Positive blood culture fate was 14.7% and the two more requested specimen per patient was high positive rate than single requested specimen. 2.Isolated rate of grain positive cocci was 23.8% and gram negative bacilli was 69.2%. 3.Isolated grain negative bacilli were Sal. group D(64.9%), E. coli(17.7%), Pseudomonas sap.(6.7%). 4.Isolated grain positive cocci were Staphylococcus aureus(55.1%), Alpa-hemolytic stretococcus(13.0%), Coagulase negative Staphylococcus spp.(9.2%), Enterococcus(8.0%). 5.Patio of annual isolation of the Salmonella spp. to total positive cultures showed a tendency of decrease and of the Enterobacteriaceae spp. and Staphyloccus aureus showed a tendency of increase. 6.Seasonal trend of Sal. group D shoved two separate peaks, summer season and winter season. Seasonal trend of Sal group A showed ore peak on August. 7.None of the Salm. spp. tested were resistant to chloramphenicol or ampicillin with Kirby-Bauer disc diffusion method and E. coli was sensitive only Cefaperazone, Gentamicin, Tobramycin arid Amikacin. Most of the isolates of S. aureus were resistant to the routinely tested antibiotics.

호산구성 백혈병의 1예 보고

함건주,정화순,김춘원,김기홍 대한임상검사정도관리학회 1979 臨床檢査와 精度管理 Vol.1 No.1

HBsAg에 오염된 혈액검체에 대한 化學的 消毒劑의 效果에 關한 硏究

함건주,이정녀,조종래,노상옥,김공현 인제대학교 1993 仁濟醫學 Vol.14 No.1

병원에서 각종 진단 목적에 이용되는 환자의 혈액 검체 내에는 세균, 바이러스 등의 병원성 미생물에 감염된 것이 많다. 따라서 본 실험은 검사를 마친 이들 검체의 대부분이 적절한 살균 조작을 거치지 않음으로써 유발될 수 있는 환경오염이나 질병의 전파 등 보건위생상 중대한 문제를 예방하고자 B형 간염 항원 양성 혈장을 사용하여 여러가지 소독제의 바이러스에 대한 불활성화 능력을 방사 면역 측정법(Radioimmunoassay, RIA)으로 측정하였다. 실험 결과는 반응 시간 7시간인 경우 2% 농도에서 benzalkonium chloride가 98.6%로 가장 높았고, glutarldehyde는 92.3%, MLDGH는 88.6%였으며, NaOCl은 68.8%의 순서로 나타났다. 따라서 2% benzalkonium chloride를 사용하는 것이 가장 효과적인 것으로 평가되었다. Contaminated blood with microorganism and virus is put into the environment without any discontamination treatment. It may give rise to a serious health and sanitation problems and also to a threat of spreading infectious disease. In order to select the appropriate disinfectant and disinfection procedure, the teats performed in vitro via artificially contaminated blood with hepatitis B surface antigen(HBs Ag) positive plasma using several disinfectants commonly used in hospitals. The tested disinfectants were sodium hypochlorite(NaOCL), sodium dichloroisocyanurate(NaDCC), ethyl alcohol(EtOH), povidon iodine, cresol soap, benzalkonium chloride, mono-laurel-di(aminoethyl)-glycin HCI(MLDGH) and glutaraldehyde. The HBs Ag inactivation study was performed using the Radioimmunoassay(RIA) method. The results were as follows ; The inactivation effect on the HBs Ag of the benzalkonium chloride, glutaraldehyde, MLDGH, and NaOCL was 98.6%. 92.3%, 88.6% and 68.8% with in 5 hours after disinfection on the same 2% concentration, respectively. In conclusion, this study provided useful information for the 2% benalkonium chloride is the best disinfectants in consideration of the chemical stability, economy, and sanitation.

Vibrio parahaemolyticus의 지방산 조성에 관한 연구

함건주,이정녀,조종래,홍성진 대한임상검사정도관리협회 1992 臨床檢査와 精度管理 Vol.14 No.2

頸動脈體 副神經節腫 1例

함건주,정화순,김춘원,박승함,김기홍 대한병리학회 1982 Journal of Pathology and Translational Medicine Vol.16 No.4

Erythroleukemia 5예

함건주,정화순,김춘원,박승함,김기홍 대한병리학회 1982 Journal of Pathology and Translational Medicine Vol.16 No.4

지방산을 투여한 마우스의 Salmonella typhi에 대한 항체 생성력

함건주,이원재,이정화,김용호 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1995 Journal of biomedical laboratory sciences Vol.1 No.1

지방산 식이가 항체 생성력에 미치는 영향을 알아 보기 위하여 ICR계 마우스를 기본사료만 투여한 대조군, 돈유 투여군, 그리고 정어리유 투여군으로 나눈 다음, 추출한 지방산을 8주동안 경구 투여한 후, 마우스 간조직 중의 지방산 조성 변화, Salmonella typhi에 대한 항체 역가, 복강내 대식세포의 탐식능 및 비장세포의 증식능을 비교 관찰한 결과는 다음과 같다. 대조군에 비하여 돈유 투여군에서는 C18:3, C20:3, C20:4 등의 지방산이 감소하였고, 정어리 투여군에서는 C18:1, C18:2/C18:0를 제외한 주요 불포화 지방산 성분이 감소되었다. 대식세포의 탐식능에서는 대조군에 비하여 실험군의 대식세포 탐식능이 저하되었고, 마우스 생체 항체 생성력은 돈유 투여군이 대조군 및 정어리유 투여군보다 높은 역가를 나타내었으며, 마우스 비장세포 배양 상층액 중의 항체 역가는 마우스 생체 역가와는 다르게 정어리유 투여군이 더 높은 역가를 나타내었다. 또한 비장세포 증식능은 지방산 투여군이 대조군보다 높았고, 그 중에서도 정어리유 투여군이 돈유 투여군보다 높은 증시능을 나타내었다. 이러한 차이는 포화, 불포화 지방산 균형 증가에 의하여 면역 억제력이 높아진 결과로 생각되며, 포화 지방산의 투여는 항체 생성력을 현저하게 증가시킬 수는 있으나 면역억제력은 나타내지 못함을 알 수 있었다. The effect of different fatty acids supplementation on antibody production of Salmonella typhi was studied in ICR mice. Subjects supplemented their diets with 50㎍ of extracted pig oil(as a saturated fatty acid) and fish oil (as a unsaturated fatty acid) / 2 days for 8 weeks. Blood was collected control and experimental groups of mice after 8 weeks of oil supplementation. The different fatty acids supplementation reduced unsaturated fatty acids composition in mice liver such as C18:3, C20:3 and C20:4 except C18:1 and C18:2/C18:0 in fish oil and pig oil groups compared to control group. Also, the phagocytic activities of mice macrophages for Candida albicans was reduced by 6% in pig oil group and 9% in fish oil group than control group. The antigen-stimulated lympocyte proliferative response was significantly increased by fatty acid in pig oil group(48%) but 57% in fish oil group. The different fatty acid supplementation increased antibody production in both experimental groups than control group ; this increase was only significant in pig oil group(1:2⁴) on mice but not in fish oil group(1:2 )compared to control group(1:2 ), however, increased antibody titer in both groups in-vitro spleen cell culture supematant(1:2³in fish oil group and 1:2²in pig oil group compared to control group 1: 2 ). Thus, fish oil supplementation was immunosuppresive agent in macrophage phagocytosis, in-vivo antibody producibilities and lympocyte proliferation but pig oil supplementation was more effective than fish oil in antibody formation in-vivo. We find that antibody producibilities affected by fed on different fatty acids were considered by balance between saturated and unsaturated fatty acid, and C20:3/C20:4 ratio. Also, it affected to antigen-stimulated lymphocyte proliferation and macrophage phagocytic activities.

철적아구성빈혈을 동반한 선천성 타원형 적혈구증 1례 보고

조종래,함건주,백인기 인제대학교 1993 仁濟醫學 Vol.14 No.1

희귀한 유전성 질환인 선천성 타원형 적혈구증과 그 발생기전이 아주 다른 Sideroblastic Anemia가 함께 나타난 한 예를 문헌고찰과 함께 보고하는 바이다. A case of hereditary elliptocytosis with sideroblastic anemia in a 9-year-old female patient is presented. The patient was admitted due to frequent nasal bleeding. Her peripheral blood smear showed elliptocytosis of about 80.5%. Her mother and sister also had elliptocytosis, 53.6% and 77.5% Respectively bone marrow aspiration smear showed sideroblastic anemia with ring sideroblasts.