Methamphetamine 이 면역장기 및 항체생성능에 미치는 영향

선우연(Woo Yearn Sun),한형미(Hyung Mee Han),윤은이(Eun Yi Yoon),신전수(Jeon Soo Shin),박현애(Hyeon Ae Park),김미영(Mi Young Kim) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.1

BALB/c mice were intraperitoneally injected with methamphetamine (5 mg/kg) to observe the effect of methamphetamine on the immune system. Body weights were decreased in both acutely treated group (twice for 2 weeks with 7 days interval) and subchronically treated group (daily injection for 14 days). The relative spleen weights and the numbers of splenocytes were unexpectedly increased (p<0.05) in acutely treated group, but subchronically treated group showed the trend of decrease without significance. But there was no significant effect on antibody formation to hen egg lysozyme which was immunized during the treatment of methamphetamine and on plaque forming cell number. The relative thymus weights of both groups were significantly decreased by the treatment of methamphetamine (acutely treated group, p<0.05; subchronically treated group, p<0.01). These results suggest that the effect of methamphetamine on the immune system may be caused by thymic dysfunction.

사염화탄소에 의하여 유발된 흰쥐의 간 독성에 미치는 비테인의 효과

김선여(Sun Yeou Kim),김홍표(Hong Pyo Kim),이미경(Mi Kyeong Lee),변순정(Soon Jeong Byun),김승희(Seung Hee Kim),문애리(A Ree Moon),한형미(Hyung Mi Han),허훈(Hoon Huh),김영중(Young Choong Kim) 대한약학회 1993 약학회지 Vol.37 No.5

Effects of betaine, a major component of Lycii Fructus, on carbon tetrachloride intoxicated rats were evaluated. Histological studies showed that betaine had improved the typical necrosis around centrilobular area in liver tissue due to the carbon tetrachloride intoxication. Betaine, whether it was administrated simultaneously or prior to carbon tetrachloride, prevented or retarded the elevation of liver-weight/body-weight ratio due to the carbon tetrachloride intoxication. Betaine also significantly elevated the reduced activities of some enzymes, cytochrome P-450, 7-ethoxycoumarin-O-deethylase and glutathione-S-transferase, involved in xenobiotic metabolism due to carbon tetrachloride intoxication. These results demonstrate a possible hepato-protective role of betaine against fatty liver that could be easily induced by carbon tetrachloride in rat.

일차 배양한 흰쥐의 간세포에서 사염화탄소로 인한 독성에 미치는 비테인의 효과

김선여(Sun Yeou Kim),김홍표(Hong Pyo Kim),이미경(Mi Kyeong Lee),김승희(Seung Hee Kim),문애리(A Ree Moon),한형미(Hyung Mi Han),허훈(Hoon Huh),김영중(Young Choong Kim) 대한약학회 1993 약학회지 Vol.37 No.5

Betaine, a major component of Lycii Fructus, was evaluated for its anti-hepatotoxic activity on carbon tetrachloride-induced hepatotoxicity in primary cultured rat hepatocytes. Betaine was found to attenuate carbon tetrachloride-induced hepatotoxicity both morphologically and biochemically. Typical hepatocyte necrosis due to carbon tetrachloride seemed to be reduced by 50 to 500mcM of betaine under microscopical observation. The value of glutamic pyruvic transaminase released from the hepatocytes into the medium significantly decreased as betaine concentration increased. Betaine also significantly elevated the reduced activities of some enzymes, cytochrome P-450, 7-ethoxycoumarin-O-deethylase and glutathione-S-transferase, involved in xenobiotic metabolism due to carbon tetrachloride-induced hepatotoxicity. These results demonstrate a possible hepato-protective role of betaine against fatty liver that could be easily induced by carbon tetrachloride.

정소에서 단백질 카르복실메칠화 반응의 기능에 대한 연구 : 돼지 정소로 부터 정제된 protein L-isoaspartyl methyltransferase의 성질에 관한 연구 Characterization of the purified protein L-isoaspartyl methyltransferase from porcine testis

정기영,신미희,한형미,강석연,김태균,홍성렬,김승희,이영근 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

Protein carboxylmethyltransferase(PCMT)의 일종인 Protein L-isoaspartyl methyltransferase(PIMt)는노화 혹은 손상된 단백질의 수복에 관여하는 것으로 보고되어 있으며 Rat의 장기별로 활성도를 비교랬을 때 특히 뇌와 정소에서 가장 높은 팔성을 보였다. 그러나 현재까지 정소에서 rIMT의 기능은 명화하게 규명죄어 있지 않으며 다만 포유동물의 정자가 수정전 몇주동안 부고환에 불활섞 상태로 저잖되는례, 이때 PIMT가 sperm protein의 구조 변형을 막음으로써 sperm의 기능과 임신능력에 영향을 미칠 것으로 생각되고 있다- 따라서 향후 PIMT의 기능 손상 과 감성 불임과꼭 상관관계를 밝히기 위해서 일차적으로 래지 정소에서 PIMT를 정제하고 그 특성을 알아보고자 하였다. 퇘지 정소를 도축장에서 구입하여 blender로 균질화한 다음 고속 원심분리와 초고속 원심분리를 이용해서 crtosol fraciion을 얻었다. of c?tosol fraction을 ammonium sulfate로 70% 포화시켜 침전물을 얻은 다음 salt를 제거하기 위해 투석하였디7. 이를 DBAE-serhacel 크로마토그래피, 읖AH-liganded 교finity 크로마토그래피,gel filtration 크로마토그래피를 하섞 SDS-PAG딘로 확인한 결과 순수정제된 PIMT를 얻있으며 분자량은 27, 0009a 정도이고 yield는 1.6%, purification fold는 1550배였다. 정제된 PIMT의 optimum pH는 6,0이었으며 isoelectric pjint(Pl)는 6.2였다. 이효소의 SAM에 대한 K- value는 1.ODf이었고 V-..는 1166.lunits였으며 WP-(L-isoAsp)-HA에 대한 K☞ value는 33.2Dtt이었고 V-..는 741.Ounits 이었으며histone type H-As에 패한 Kf value는 276Df 이었으며 Vma.는 502.Sun죠5 이었다. Protein L-isoaspartyl methyltransferase(PfMT) is Eelieved to 13e play an important role in the disposition of age-rlamaged proteins by catatyzing the repair of abnormal isoaspartyl linkagesresulting flora the spontaneous deamidatioB of asparaginyt resiaues or isomeriBation of aspartylresidues. Although PIMT is widely distributed in maramalian tissues, its activity is especially high intestis. So far, Testicular PIWT could be invoved in either sperm motility or sperm maturation. Thereforeas a initiat step toward caarifying the function of protein carboxfrlmethylation in testis we purifiedPIMT from porcine testis as a monomeric 27,000 Da species by ammoBium sulfate precipftation, DEAE-sephacel chromatography, SAH-liganded affinity chromatography, and gel filtration chromatography.Isoelectric focusing of puril'ied PIMT showed a single protein species with an isoelectric point of 6.2. Theoptimum Bf for the reacti㉠n was 6.0. Km values of the enByme for the S-adenosyl-L- methionine(SAM) ,synthetif'"'ftigopeptide(VYP-L- isoD-HA ) and histone type ll -As were 1.ODf, 33.2ff and 276Dfrespectively.

DHEA의 항비만 효능 및 ob 유전자 (leptin)의 발현에 미치는 영향

정기경,신미희,한형미,강석연,김태균,강주혜,문애리,김승희 德成女子大學校 藥學硏究所 2000 藥學論文誌 Vol.11 No.1

Leptin, the product of the ob gene, is a small peptide molecule synthesized by white adipocytes with an important role in the regulation of body fat and food intake. Baesd on the evidence that synthesis of leptin is regulated by female sex hormone, estrogen, this present study was investigated whether sex hormone precursor, DHEA, can regulate obese gene expression in lean and genetically obese (ob/ob) mice. Antiobesity activity of DHEA was evaluated by determining body weight, food consumption, epididymal fat weight and serum levels of cholesterol and triglyceride in ICR, C57BL/6J, and ob/ob mice. The treatment of C57BL/6J lean and obese mice with a diet containing 0.3% and 0.6% DHEA resulted in lowered rates of weight gain in comparison to non-treated mice, although much greater response was found in the obese mice. All other concentrations of DHEA (0.015%, 0.06%, 0.15%, 0.3%) except the highest one(0.6%) showed no significant effects on weight gain in ICR mice. Food consumption was significantly decreased in all mice treated with 0.6% DHEA, whereas it was not decreased in ICR mice at lower concentrations than 0.6% DHEA. DHEA decreased significantly epididymal adipose tissue weight and serum triglyceride levels dose dependently in lean and obese mice. However, serum cholesterol levels were decreased at lower concentrations than 0.15% DHEA and increased at concentrations of 0.3% and 0.6% DHEA in lean and obese mice. These increases in serum cholestrol levels at high concentrations of DHEA might result from the fact that DHEA has a cholesterol moiety, thereby interfered the assay system. As an approach to elucidate the mechanism for antiobesity activity of DHEA, we examined mRNA levels of obese gene in the adipocyte and obese gene product (leptin) in the serum. The results showed that DHEA did not affect obese gene expression in ICR and C57BL/6J mice. Therefore, we concluded that antiobesity activity of DHEA was not modulated by obese gene expression.