食中毒에 對한 漢方療法

崔春根 藥業新聞社 1981 (月刊) 醫藥情報 Vol.1981 No.6

蛋白尿의 漢方療法

崔春根 現代醫學社 1965 現代醫學 Vol.2 No.-

漢藥業士되기 위해 한의원에 종사하는 무자격자들이 문제다

崔春根 臨床醫學社 1987 臨床醫學 Vol.1987 No.9

쓰름매미 (Meimuna mongolica D.) 복안의 전자현미경적 관찰

최춘근,유관희,신길상,최임순,Choi, Choon-Keun,You, Kwan-Hee,Shin, Kil-Sang,Choe, Rim-Soon 한국현미경학회 1979 Applied microscopy Vol.9 No.1

Electron microscopic studies were carried out to investigate the fine structure of ommatidia of the compound eyes in Meimuna mongolica D. The crystalline cone appears round, and is composed of four double-membraned cone cells and surrounded by pigment cells having many pigment granules. The rhabdom is a closed type, and is composed of four rhabdomeres Its cross section reveals lamellated microvilli which are oriented in four different directions suggesting that these represent four photoreceptive sites. The microvilli, in a cross sectional view, are hexagonal in shape with a central axis inside. There are usually eight retinular cells arranged radially from the rhabdom, but in some retinular layers seven or nine retinular cells could be observed. The cytoplasm of each retinular cell is interconnected with that of microvilli of the rhabdomere, but the appearance of this interconnection varies depending on the number of retinular cells.'The retinlilar cells neighboring the microvilli seem to have well-developed perirhabdomal vacuoles and mitochondria as well as pigment granules surrounding these vacuoles.

Actinomycin D가 거세된 휜쥐 섭호선의 미세구조에 미치는 영향

최춘근,고기석,이재익 연세대학교 자연과학연구소 1985 學術論文集 Vol.15 No.-

정상인 흰쥐의 섭호선 조직과 거세를 시켜서 androgen의 영향을 억제시킨 흰쥐의 섭호선 조직, 그리고 거세시킨 후 actinomycin D를 투여하여 이와 같은 항생물질이 섭호선에 주는 미세구조의 변화를 관찰하고 위의 각 군에서 조직화학적인 방법을 이용하여 acid phosphatase의 반응을 전자 현미경으로 관찰하였다. 이 결과 거세시킨 흰쥐의 섭호선 세포에서 세포소기관의 소멸, 미세융모의 퇴화등 변화를 나타내었고, androgen의 영향이 없을 경우 세포의 대사이상을 나타내었고, actinomycin D를 처리하였을 경우 핵의 chromatin mass의 증가, 세포막의 파괴와 라이소좀이 증가되는 경향을 나타내었다. acid phosphatase의 반응은 거세된 흰쥐 세포에서 핵과 세포질에 분포하나 actinomycin D를 투여한 군에서는 주로 핵에 분포하는 경향을 나타내었다. Ultrastructural changes of prostates of the normal rats, castrated rats and actinomycin D treated castrated rats are studied with electron microscope. In addition, reaction of acid phosphatase is studied by histochemical method in each group. Disappearance of cell organells, (mitochondria, ER) degeneration of microvilli are characteristically observed in the castrated rat prostate. Increase of chromatin mass, destruction of cell membrane are seen in the actinomycin D treated castrated rat prostate. Reaction products of acid phosphatase are ubiquitous in nucleus and cytoplasm of the castrated rat prostate, but this reaction is predominant in nucleus of the actinomycin D treated castrated rat prostate.

방사선에 조사된 생쥐의 정소에 미치는 Cysteine의 방어 효과에 관한 현미경적 연구

최춘근,유관희 연세대학교 대학원 1980 延世論叢 Vol.16 No.2

This study was made to investigate the radioprotective effects of cysteine on the gamma irra diation injury, by means of electron microscopic examination of the testis of mile treated by cysteine before whole body irradiation. Experimental results obtained are as follows: 1. At 30 minutes after irradiation, the nucleus of spermatogonia began to shrink and round swollen mitochondria of spermatocytes were observed in saline treated group, but cysteine treated group showed slight decrease of cytoplasmic granules. 2. At 2 hours after irradiation, spermatocytes show destruction of nuclear membrane and irregular karyoplasm which was caused by aggregations of chromatin of nucleus in saline treated group. It was also observed aggregation of chromatic in cysteine treated group, but they showed lower appearance than saline treated group. 3. In the group of 9 hours after irradiation, the saline treated group appeared large vacuoles in the cytoplasm and irregular electron dense structures in the nucleus and showed significant aggregation of chromatin. And segregation of nucleolus, aggregations of chromatin were observed in cysteine treated group.

Mouse 적혈구의 Glutathione Peroxidase의 순화 및 성질에 관한 연구

崔春根,李成淑 연세대학교 자연과학연구소 1982 學術論文集 Vol.9 No.-

South ICR strain mouse의 적혈구로부터 glutathione peroxidase를 (NH_4)SO_4 침전법, Sephadex filtration columns와 DEAE-Sephadex column chromatography를 이용하여 약 32.4배 순화하였다. 효소액이 완전히 순화된 것을 Sephadex G-200 column chromatography에 의해서 알 수 있다. Crude glutathione peroxidase의 최적온도는 40℃이며, 반응 최적 pH는 7.5이다. 또 여러 온도에서 10분간 가열하였을 때 crude glutathione peroxidase는 30℃에서 가장 안정하였으며, 60℃까지는 효소 활성이 서서히 감소한다. 그보다 높은 온도에서는 급격히 효소 활성을 잃었다. Crude glutathione peroxidase의 기질 glutathione에 대한 Km값은 8.3mM이고, 최대 반응 속도는 15.4 units/ml이었으며, H_2O_2에 대한 Km값과 최대 반응 속도는 각각 40μM과 10.5units/ml로 측정되었다. Sephadex G-200 gel filtration을 이용하여 측정한 glutathione의 peroxidase 분자량은 약 84,000 정도이다. A glutathione peroxidase was purified approximately 32.4-folds from south ICR strain of mouse erythrocytes by ammonium sulfate precipitation, Sephadex filtration columns and DEAE-Sephadex column chromatography. The purified enzyme was appeared homogeneous in the Sephade G-200 column chromatography. The optimum temperature of the crude glutation peroxidase was at 40℃, and the pH was at 7.5 After heating at various temperatures for 10 min, the crude glutathione peroxidase was most stable at 30℃ and the activity was declined slowly until 60℃ and rapidly at high temperature. The values of Km and Vmax of the crude glutathione peroxidase were calculated to be 8.3mM and 15.4 units/ml for glutathione and for hydrogen peroxide 40μM and 10.5 units/ml respectively. The molecular weight of glutathione peroxidase was estimated by Sephadex G-200 gel filtration and it was approximately 84,000.

한국산 Planaria의 인두 세포내 핵포함체의 전자현미경적 연구

최춘근,전진석 연세대학교 대학원 1980 延世論叢 Vol.17 No.1

Light microscopic observation dating back to 1896 haute established that nuclear inclusions occur widely in animal and plant cells. This investigation was conducted to study the ultrastructure of the intranuclear crystals in the pharyngeal cells of Planaria (Dugesia gonocephala). The pharyngeal cells of Planaria was observed through a light microscope and electron microscope and the results of the observation are as follows: 1. The parenchymal tissue layer which occupies most of the center of pharyngeal wall, consists mainly of mesenchymal cells and gland cells, and is filled with granules. 2. The form of the cells having nuclear inclusions is a semicircle with the nuclei lopsided to one side and a high electron density of chromatin was found in nucleoplasm and evenly distributed in the nuclei 3. The nuclear inclusions is in an oblong form without any limiting membrane that decides on structures but the parallel portion toward the direction with subunits was conspicuous in boundary with nucleoplasm. 4. In this experiment light microscopy failed to reseal evidence of crystalloid structures in the pharyngeal cells of any of the specimens but the histological structures of pharynx were clarified. In contrast, electron microscopic examination disclosed the occasional presence of formations with a crystalloid appearance in the nucleus of pharyngeal cells. 5. The crystalloid consists of many tubular elements running parallel to the long axis embeded in dense fine granular material. Dense granules resembling ribosomes were linearly disposed in the interfilamentous spaces. Also no limiting membrane of intranu-clear inclusions was observed. 6. An acumulated phenomenon of glycogen granules was observed in the cytoplasm of cells with nuclear inclusions.

Dibutyryl Cyclic AMP가 생쥐여포난자의 성숙에 미치는 억제효과에 관한 자기방사법적 연구

최춘근,Choi, Choon-Keun 한국현미경학회 1977 Applied microscopy Vol.7 No.1

This experiment was undertaken in order to localize the labeled dbcAMP (dibutyryl cyclic AMP) in oocytes whose development has been suppressed by cold dbcAMP for 6 or 19 hours in vitro. Mouse oocytes were obtained from the ovaries of 3-4 week old A strain female mice, by puncturing the Graafian follicles in the modified Krebs-Ringer bicarbonate salt solution under the dissecting microscope. Those oocytes which have intact germinal vesicle were cultured in the basic culture medium supplemented with 0.4% bovine serum albumin (BSA). Cultivation of the oocytes was carried out in a microtube developed by Cho (1974). The cultures were then incubated in a humidified 5% $CO_2$ incubator maintained at $37^{\circ}C$ for 6 or 19 hours (Donahue, 1968). DbcAMP was added to culture medium for a final concentration of 100ug/ml, and $^3H-dbc$ AMP (specific activity 13 Ci/mM) for a final concentration of $40{\mu}Ci/ml$ was also added to the medium. For electron microscopic autoradiography, those oocytes recovered from the culture were washed with phosphate buffer (pH 7.4), and immediately prefixed in a 2.5% glutaraldehyde overnight and postfixed for 2 hours at $4 ^{\circ}C$ in 1% osmium tetroxide in phosphate buffer with pH 7.4 (Palade, 1952). After fixation, the materials were dehydrated in graded alcohol series and embedded in Epon 812 mixture based on the standard procedures (Luft, 1961). The thin sections $600-700{\AA}$ thick were mounted on the grids of 200 meshes. The grids containing sections were coated with a nuclear emulsion Kodak NTB-3 and stored in a cold dark box (at $4^{\circ}C$) for 3 weeks. After exposure, the samples were developed with Kodak D-19 and stained with uranyl acetate and lead citrate. Routine observation was made with Hitachi HU-11E electron microsocope. The results of the observation were as followings: 1. It was found that the labeled dbcAMP penetrated the egg plasma membrane and dispersed at random in the cytoplasm. 2. It was also observed that most of the labeled dbcAMP was attached to microfibrillar lattices portion of the oocyte cytoplasm. There fore, it is presumed that the receptor of the dbcAMP is localized in the microfibrillar lattices of the oocyte. 3. It also seems that some other cell organells such as mitochondria, Golgi complex, cortical granules are not directly related to the action of the dbcAMP. 4. The labeled dbcAMP was neither observed in the membrane nor in the nucleus. Therefore, it seems that there is no relationship between the concentration of dbcAMP and the nuclear membranous permeability. 5. There was no difference in number of dbcAMP particles when oocytes were cultured for 6 hours and 19 hours. 6. However, it was observed that, in same of the oocytes suppressed in germinal vesicle by dbcAMP for 19 hours, cell organells were moved and concentrated to a small portion of the cytoplasm, and that the morphology of the organells greatly changed to an abnormal. form. Therefore, it is supposed that those oocytes were in the process of degeneration. From the above results, it is expected that dbcAMP penetrated the egg membrane and was bound to the receptor which seems to be located in the microfibrillar lattiees portion, and that this dbcAMP-receptor complex inhibited some enzyme system of the oocytes which are essential for the germinal vesicle breakdown.

신입생의 변

최춘근 언론중재위원회 1999 言論仲裁 Vol.70 No.-