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이차원 수소자기공명분광법을 이용한 BPTI의 공명주파수 지정
한규훈,정동호,박규환,채동연,김수경,유현주,Han, Kyou-Hoon,Choung, Dong-Ho,Park, Kyu-Hwan,Chae, Dong-Yeun,Kim, Soo-Kyung,Yoo, Hyun-Ju 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5
BPTI(Basic Pancreatic Trypsin Inhibitor)에 phase-sensitive COSY(shift COrrelation SpectroscopY), DQF(Double Quantum Filtered)-COSY, TOCSY(TOtal shift Correlation SpectroscopY), NOESY(two-dimensional NOE SpectroscopY) 등의 2차원 핵자기공명분광법을 사용하여 수소 공명주파수를 지정하고 $^{3}J_{HNa}$ 값을 측정하여 기존 문헌치(Wagner et al., 1987; Berndt et al., 1992)와 비교한 결과 chemical shift 값, 공명주파수 지정에 사용된 순차적 NOE 및 $^{3}J_{HNa}$ 값 등이 거의 일치한다. 한편, 본 연구에서는 기존 문헌에서 지정하지 않은 14개의 수소 공명주파수를 추가로 지정할 수 있었다. Two-dimensional NMR methods such as phase-sensitive COSY (shift COrrelation SpectroscopY), DQF (Double Quantum Filtered)-COSY, TOCSY (TOtal shift Correlation SpectroscopY), NOESY (two-dimensional NOE SpectroscopY) in $H_{2}O$ and $D_{2}O$ have been used to achieve resonance assignment for basic pancreatic trypsin inhibitor. Results are in good agreement with the previous reports by Wuthrich and his coworkers (Wagner et al., 1987; Bemdt et al., 1992). In addition, in this work, we have assigned 14 additional resonances that were not assigned before.
이차원 수소자기공명분광법을 이용한 BPTI 의 공명주파수 지정
한규훈,정동호,박규환,채동연,김수경,유현주 ( Kyou Hoon Han,Dong Ho Choung,Kyu Kwan Park,Dong Yeun Chae,Soo Kyung Kim,Hyun Ju Yoo ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.5
Two-dimensional NMR methods such as phase-sensitive COSY (shift Correlation SpectroscopY), DQF (Double Quantum Filtered)-COSY, TOCSY (Total shift Correlation SpectroscopY), NOESY (two-dimensional NOE Spectroscopy) in H₂O and D₂O have been used to achieve resonance assignment for basic pancreatic trypsin inhibitor. Results are in good agreement with the previous reports by Wu¨thrich and his coworkers (Wagner et al., 1987; Bemdt et al., 1992). In addition, in this work, we have assigned 14 additional resonances that were not assigned before.