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OPU 유래 수정란의 대량생산을 위한 고능력 공란우 반복사용 가능성에 관한 연구
진종인,최병현,김성수,박번영,이정규,공일근 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.3
This study was designed to know the possibility in repeat uses of elite donor cows for getting mass production of OPU-derived embryo production (OPU-IVP). Ultrasound transvaginal ovum pick-up (OPU) performed in 6 Korean native cows was aged 4 to 10 years old. The aspiration of immature oocytes for OPU derived embryo was carried out 2 times per week, and OPU-IVP of 1st period was carried out 22∼48 sessions from each donors. And the break time for OPU-IVP of 2nd period after 1st OPU from each donors were 2∼25 months. The OPU-IVP of 2nd period each donors conducted total 15∼65 times for 2∼8 months by an ultrasonographic, was guided follicular aspiration system. The average numbers of collected oocytes, grade 1 + grade 2(G1+G2) oocytes and cleavage embryo from 1st period OPU-IVP were significantly differences between donors (p<0.05). Total collected oocytes of donor D were significantly higher compared with donors of A, B, C, E and F (average 17.0 per session vs. 11.2, 10.1, 8.5, 10.2 and 9.6; p<0.05) and also oocytes of G1+G2 were significantly higher compared with r A and D and subsequently to donors of B, C, E and F (average 7.9 and 8.5 per session vs. 5.0, 2.7, 6.0 and 1.6; p<0.05). Cleavage rate of donor D was significantly higher compared with donors of A, B, C, E and F (average 13.1 per session vs. 10.1, 9.1, 6.9, 8.9 and 6.7; p<0.05). The average numbers of OPU-IVP for 1st period was significantly higher from donors of B, D and E than those from donors of A, C and F (average 6.5, 7.1 and 6.5 per session vs. 3.5, 4.2 and 2.8; p<0.05). The possibility investigation of 2nd OPU-IVP was carried out after 2∼25 months rest periods from 1st period OPU session. Total average numbers of collected oocytes, cleavages and blastocyst development rates were significantly higher from 1st period OPU compared with 2nd period one (p<0.05). The OPU-IVP efficiency by break for more embryo production from elite cow was analysis comparing without rest of donor A, under 6 months rest period as B and over 6 months rest period as C and then the average numbers of collected oocytes, cleavages and blastocysts were significantly higher from A group (11.8, 9.5 and 5.2 per session) than those from B and C groups (7.9, 6.2 and 2.6 vs. 9.2, 7.5 and 3.9, p<0.05), and also C group was significantly higher than B group. In conclusion, 1st period OPU-IVP was more efficient compared with 2nd period repeated uses of donor, and the break times for additional production of embryo on donor were needed more than over 6 months after 1st period OPU-IVP. This repeating uses of elite donor cows given more emphasis for getting the opportunity on mass production of elite cow OPU-IVP embryo should be increased G1+G2 possibility of genetic improvement of livestock within short period.
간호대학생의 투지(Grit), 학업탄력성 및 심리적 안녕감
진종임(Jin, Jong Im),김남초(Kim, Nam Cho) 한국간호교육학회 2017 한국간호교육학회지 Vol.23 No.2
Purpose: This study was conducted to investigate the relationship among grit, academic resilience, and psychological well-being among nursing students. Methods: The participants were 271 nursing students, enrolled in C university. Data were collected from September 5 to 30, 2016 and analyzed using descriptive analysis, t-test, ANOVA, Pearson correlation coefficients, and stepwise multiple regression with the SPSS/WIN 21.0 program. Results: The mean scores for academic resilience were above average. The mean score for grit and psychological well-being presented an appropriate level. Psychological well-being in nursing students was significantly correlated with academic resilience (r=.65, p<.001) and grit (r=.52, p<.001). The significant predictors of psychological well-being for nursing students were academic resilience (β=.47, p<.001), grit (β=.26, p<.001), and major satisfaction (β=.20, p<.001) which explained 53.1% of the variance in psychological well-being. Conclusion: The results indicate that it is necessary to develop grit and academic resilience enhancement programs to improve the psychological well-being of nursing students.
Pitch Measurement of 150 nm ID-grating Standards Using an Nanometrological Atomic Force Microscope
진종한,Ichiko Misumi,Satoshi Gonda,Tomizo Kurosawa 한국정밀공학회 2004 International Journal of Precision Engineering and Vol.5 No.3
Pitch measurements of 150 nm one-dimensional grating standards were carried out using a contact mode atomic force microscopy with a high resolution three-axis laser interferometer. This measurement technique was named as the nano-metrological AFM. In the nano-metrological AFM, three laser interferometers were aligned precisely to the end of an AFM tip. Laser sources of the three-axis laser interferometer in the nano-metrological AFM were calibrated with an I2-stabilized He-Ne laser at a wavelength of 633 nm. Therefore, the Abbe error was minimized and the result of the pitch measurement using the nano-metrological AFM could be used to directly measure the length standard. The uncertainty in the pitch measurement was estimated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM). The primary source of uncertainty in the pitch-measurements was derived from the repeatability of the pitch-measurements, and its value was about 0.186 nm. The average pitch value was 146.65 nm and the combined standard uncertainty was less than 0.262 nm. It is suggested that the metrological AFM is a useful tool for the nano-metrological standard calibration.