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배지최적화를 통한 재조합 바실러스 서브틸리스에서 바실러스 아밀로리퀴파시엔스 유래 γ-글루타밀펩타이드전달효소의 대량생산
조혜빈(Hye-Bin Cho),제텐드라 쿠마르 로이(Jetendra Kumar Roy),박우진(Wu-Jin Park),전병운(Byoung-Oon Jeon),김영완(Young-Wan Kim) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.6
본 연구를 통해 BAGGT를 재조합 B. subtilis를 이용하여 대량 생산하기 위하여 유전자 클로닝, 발현 시스템 구축 및 배지 최적화를 진행하였다. 이중프로모터 시스템을 이용하여 야생형 균주에 비해 42배 효소 생산성이 향상된 발현 시스템을 구축하였다. 또한 PBD 분석을 통해 당밀과 CSL이 재조합 B. subtilis 시스템에서 BAGGT의 생산성에 큰 영향을 주는 인자임을 확인하였으며, 염류의 첨가에 의한 효소 생산성 증대 효과는 미비하거나 부정적이었다. 탄소원으로 당밀을 선택하고 고가의 질소원인 트립톤을 저가의 CSL로 교체한 후 CCD 분석을 통해서 결정된 최적 배지 사용 시 최적화 이전의 LB 배지 대비 4.3배의 생산성 증대를 이루었으며, 이는 LB 배지에서 야생형 균주의 BAGGT 생산성 대비 180배의 효소 생산성 개선에 해당하였다. 본 연구를 통해 식품용 효소로서 BAGGT의 대량생산을 위한 공정을 구축하였으며, 이후 정미성 소재 생산에 활용할 수 있을 것으로 기대한다. γ-Glutamyltranspeptidase (GGT, EC 2.3.2.2) transfers γ-glutamyl moiety from glutamine to amino acids or peptides and hydrolyzes glutamine to glutamate and ammonia. In order to overproduce γ-glutamyltranspeptidase from Bacillus amyloliquefaciens (BAGGT), the encoding gene was cloned and expressed in Bacillus subtilis. The productivity of BAGGT in Bacillus subtilis was improved by 42-fold by using a dual-promoter system that was generated by combining promoters from B. subtilis α-amylase and BAGGT genes. Through optimization of medium composition by Plackett-Burman design and central composition design, BAGGT was produced at 18.3×107 U/L of culture in the optimized medium. Compared to previously used Luria-Bertani medium, the optimized culture medium (15 g/L molasses, 60 g/L corn steep liquor, 6 g/L yeast extract, 4 g/L NaCl, 6 g/L K2HPO4, and 2 g/L KH2PO4), resulted in a 4.3-fold increase in production of BAGGT.
형태적 구성방법에 따른 전이공간에 관한 연구 -초등학교를 중심으로-
조혜빈(Cho, Hye-Bin),원호성(Won, Ho-Sung) 대한건축학회 2022 대한건축학회 학술발표대회 논문집 Vol.42 No.1
Industrialization and urbanization proceeded with technological development. The face of society is also constantly changing. As the way of life of human beings also changes, the space is also changing into various forms. Accordingly, the transition space is also changing. However, it is not simply a public space that connects private spaces, but is changing into a third space with its own character. The characteristics of the transition space were analyzed by dividing the design competition into three types, centering on the elementary school. The corridor, which used to be a moving space in the old school, has been transformed into a space with a new character. In addition, an integrated, intrusive, and overlapping space that is not partitioned into a square classroom was created. This means that changes have come to the transitional space of the educational space. Through this, it can be referred to in planning an efficient and creative transition space when planning the construction of educational facilities.
엄인철,조혜빈,강경돈,Um, In-Chul,Cho, Hye-Bin,Kang, Gyung-Don 한국섬유공학회 2009 한국섬유공학회지 Vol.46 No.5
In this study, hydroxypropyl methylcellulose (HPMC) filaments were prepared by the wet spinning process, and the coagulation behaviors of HPMC dope and the morphology of wet spun HPMC filaments were investigated. THF, acetone, and iso-propanol showed a partial coagulation strength for HPMC dope though they did not produce a filament form. On the other hand, a high temperature water could coagulate the HPMC dope due to the gelation character of HPMC solution. However, the temporarily coagulated HPMC filament returned to a solution state when it came out of the coagulation bath. This problem, due to the reversible gelation behavior of HPMC, could be solved by adding salts in the water (coagulant). As the salt concentration increased in the coagulant, the coagulation strength was improved resulting in a reduced fiber forming temperature. Furthermore, ammonium sulfate was turned out to be the most effective salt to enhance the coagulation of HPMC in aqueous salt solution. The thermo-rheological measurements revealed that ammonium sulfate was the most effective in reducing the flocculation and the gelation temperatures among salts tested. The HPMC filament could be produced finally with the help of second coagulation bath containing organic solvent. Among the organic solvents, isopropanol was the best coagulant yielding the highest post drawing ratio of HPMC filament. The SEM observation indicated that the wet spun HPMC filament had irregular surface and cross section. The surface of HPMC could be smoother when the salt concentration was reduced to 8%.