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신경독성물질인 6-aminonicotinamide가 뇌신경원종양세포의 세포사에 미치는 영향
본 연구에서는 신경독성물질의 일종인 6-AN을 인간 뇌신경원종양세포(neuroblastoma)인 IMR32에 단독투여하여 세포의 분열 및 세포사, 그와 관련된 단백질 변화 등을 추적하였다. 6-AN의 생화학적 기능을 이해하기 위하여 6-AN 대사 기능과 직결된 ATP와 NAD를 첨가한 그룹을 사용하였다. 6-AN에 의한 세포의 생장율은 control 그룹에 비해 급격하게 감소하였으나, 6-AN과 NAD를 처리한 그룹의 생존율은 복귀되었다. Apoptosis와 관련된 효소인 PARP는 6-AN에 의해 절단이 일어나 불활성화되었고, ATP와 NAD는 그 현상을 막지 못하였다. Agarose 젤 전기영동을 통한 DNA 분절 실험에서 분절이 나타나지 않아 6-AN에 의한 DNA의 oligonucleosome 절단이 일어나지 않음을 알 수 있었다. 효소 활성에서 glyceraldehyde-3-phosphate dehydrogenase, cytochrome c oxidase와 succinate dehydrogenase가 6-AN에 의하여 활성이 감소되었으나 항산화제 효소인 catalase와 superoxide dismutase의 활성에는 영향을 끼치지 않았다. 전자현미경 관찰에서 세포는 6-AN에 의한 apoptosis 특징인 분열되고 있는 핵이 나타났으나 세포질의 파괴가 먼저 일어나고 핵이 파열되는 necrosis의 특징이 동시에 나타나 aponecrosis 현상이 일어남을 관찰하였다. 원활한 apoptosis 과정의 진행에서는 세포내 충분한 ATP가 필요하지만 6-AN은 이러한 ATP 합성을 저해함으로써 apoptosis의 진행을 방해하여 세포사의 형태가 apoptosis에서 necrosis로 넘어갔을 것으로 여겨진다. 즉, 0.5 mM 6-AN의 72시간 처리는 IMR32 뇌신경원종양세포에서 ATP 합성을 방해함으로써 aponecrosis을 유도한다. 그러나 첨가된 ATP는 6-AN에 의한 세포사에 영향을 미치지 못하였으며, NAD는 약간의 회복을 나타내는 실험도 있었으나 완전하게 해독되지는 않는 것으로 나타났다. 따라서 처리되는 6-AN의 농도와 시간이 적절하게 조절되고 apoptosis와 necrosis 단계에 대한 flow cytometry를 이용한 phosphatidylserine(PS) 분석, ELISA 분석 등의 더 많은 실험이 이루어진다면 정상세포에 최소한의 손상을 주는 효과적인 항암치료가 가능할 것으로 보인다. This study was conducted to investigate the effect of neurotoxin 6-aminonicotinamide(6-AN) on the death of cell and profiles of altered changes associated with the cellular phenomenon. For better understanding of 6-AN's metabolic effect the additional test groups such as 6-AN+ATP and 6-AN+NAD groups was supplemented. The survival rate of cells treated with 6-AN decreased dramatically compared to the control group. But the addition of NAD to 6-AN treated cells promoted to bring survival rates close to those of the control group. The Western blot analysis showed the poly(ADP-ribose)polymerase(PARP) was cleaved in to 84 kbp fragment by 6-AN. DNA fragmentation did not occur by electrophoretic analysis of DNA laddering in all four groups. The activities of glyceraldehyde-3-phosphate dehydrogenase, cytochrome c oxidase and succinate dehydrogenase decreased but those of catalase and superoxide dismutase were not affected. Electron micrograph revealed that cells consisted of mixed populations of cells with typical apoptotic features and those with apoptotic and necrotic features. This new type of cell death termed aponecrosis was detected in the present study. Aponecrosis could account for an incomplete execution of the apoptotic program and the following degeneration in necrosis. The levels of ATP were a primary determinant in directing toward apoptosis, aponecrosis, or necrosis. Because ATP was depleted by 6-AN, the switch of cell death mode from apoptosis to necrosis occurred in IMR32 cells treated with 0.5 mM 6-AN for 72 hours.
독립청구항과 종속청구항 구분 기준에 대한 고찰 : 한국 및 미국 판례를 중심으로
청구항은 주어진 과제를 해결하기 위하여 구성요소(構成要素 Elements)와 한정사(限定詞 Limitations)를 유기적으로 결합한 하나의 기술적 아이디어를 문자로 표현한 것이다. 청구범위(Claims)는 청구항이 1 또는 2 이상 기재되어 특허출원서에 제출된 것을 말한다. 청구항은 기계, 제조물, 방법 또는 이러한 것들의 조합(Combination)으로서 발명에 대한 개념을 문자로 정의함으로서 이 문자를 해석함으로서 특허가 가지는 권리범위를 설정한다. 청구항은 두 가지의 역할을 한다. 첫째는 특허권자의 권리를 보호하는 것으로서, 출원된 발명이 특허를 받을 수 있는 대상이 될 수 있는지, 누가 진정한 발명자인지, 신규성과 신규성의 상실사유가 있는지, 진보성 및 공개요건을 충족하였는지 여부를 판단하기 위하여 필요한 발명의 개념을 정의한다. 둘째는 일반대중에게 공시하는 역할로서, 특허권자의 허락 없이 특허발명을 실시하는 행위 즉 침해행위가 무엇인지를 규정하기 위한 개념을 정의한다. 특허의 청구항은 독립청구항이나 종속청구항의 형식으로 작성된다. 독립청구항은 독자적으로 다른 청구항을 인용함이 없이 출원된 발명을 개념정의한다. 이에 비하여 종속청구항은 그 이전의 청구항이 가지는 보다 넓은 발명개념을 언급한 뒤 그 보다 권리범위를 좁히는 한정사를 추가한다 독립청구항과 종속청구항을 구분하는 데 있어서 가장 중요한 기준은 각 청구항의 ‘한정사’를 어떻게 해석하느냐에 달려 있다. 원칙적으로 종속청구항은 그를 종속시키는 독립청구항이나 종속청구항의 모든 한정사 전체를 그대로 인용함으로써 특허대상을 더욱 한정할 수 있다. 하지만, 실 사례에서는 단순히 문언적 형태로만 판단하기에 부족한 점이 있어서 한국 및 미국의 판례를 바탕으로 형식설, 실질설 2가지 형태로 구분을 하여 보았다. A Claim shows one technical idea organically combining elements and limitations by using character for the sake of solving the ascribed task. Claims(claim scope) usually mean the claim submitted to a patent application including one or more written claims. In other words, a claim defines a concept of invention coming from machine, product, method and the combinations as character or letter, which can set the extent of a patent right through translating the characters. A Claim has two functions. One is to protect a patentee's right, which defines the concept of invention to judge the probability of getting the patent right, the concerned original inventer, novelty and loss reason of novelty, an adequate level for non-obviousness and disclosure requirements. The other is to publicly announce a patentee's right, which results in setting the meaning of implementation without patentee's permission (infringements) A Claim is composed of independent claim and dependent claim. The former can independently define the patent application without quoting other claim. On the other hand, the latter comments the wider concept of invention belongs to the former claim and afterwards adds limitations resulting in narrowing the patent right scope. The most important criteria of classification for independent claim and dependent claim depend upon the way to translate limitations. In principle, dependent claim can limit the concerned invention more seriously by citing all limitations literally. Nevertheless, there is an inadequate point in real case when deciding the patentee's right scope solely through literal form. Consequently, I classify 2 standards for assortment of independent claim and dependent claim by analyzing legal case in Korea and U.S.A.
치매노인 부양가족의 부적응에 관한 연구 : 가족탄력모델을 중심으로
This paper’s aim was to research the maladaptation and difficulties in the adjustment process of family members of Alzheimer’s patients based on McCabbin’s “Resiliency Model of Family Stress, Adjustment and Adaptation”. The research was carried out under the premise that the maladjustment of family members due to the suffering of Alzheimer’s patients does not only greatly affect the family’s own welfare but also produces negative effects for the treatment and rehabilitation of the Alzheimer’s patient him- or herself. This research sought to find the factors that greatly influence the maladjustment of family members of Alzheimer’s patients. Another purpose was to analyze the amount of influence given by these factors and throw some light onto whether these factors can be applied to other Alzheimer’s patient families. 1. The subjects for the research were family members (mainly spouses and children) of patients officially diagnosed with Alzheimer’s disease who are 60 years old or older. 2. The establishments and facilities that were investigated were the Ulsan Metropolitan City Alzheimer’s Disease Support Center, the Ulsan City Senior Hospital, one general hospital, two oriental medicine hospitals, 11 Senior Day Care Centers, 3 Senior Citizen Short-Term Care Centers, Alzheimer’s Counseling Centers (Public Health Centers for the Junggu, Namgu, Donggu, Bukgu and Wuljugun Districts in Ulsan City), 11 Public Health Treatment Centers within the Wuljugun District and other Alzheimer’s support centers. This research also investigated Alzheimer’s patients who have been admitted and discharged from 10 Senior Citizen Nursing Homes, patients who have been temporarily admitted to nursing homes, patients who were monitored at Alzheimer’s Counseling Centers and a few Alzheimer’s patients who are treated at home. Medium- to small-sized hospitals and clinics were not cooperative in the research surveys, hence Alzheimer’s patients at these facilities were not readily accessible. As a result, the subjects of my research are limited to those within the Ulsan region. Therefore, the survey results hardly represent the family resiliency of family members of the entire population of Alzheimer’s patients in Korea. Based on the results of my research I have come up with the following proposal to help families of Alzheimer patients using Social Work Intervention Methods. Initially, in order to foresee, prevent and carry out therapeutic intervention methods to treat maladaptation of Alzheimer patients' families, it is necessary to perform a thorough review and examination of the family's capability in exercising emotional control, and communication methods as well as interaction and support obtained from the extended family. If Social Work Intervention Methods are considered to be imperative after the examination, the expert should help the family improve its emotional control, enhance interaction and the acquisition of support from the extended family members and plan social work intervention means in order to educate and instill more effective communication skills among the patients' family members. First, in order to encourage efficient communication skills between the patient and the patient's family (in other words, enhance favorable communication methods that are not resentful to the counterpart), we can use either the Behavior Rehearsal Technique or the Role Exchange Technique. By using the Role Exchange Technique, which makes family members exchange roles, allowing them to get first-hand experience on how the use of bitter language and hostile communication methods can influence as well as help them gain clear insight into effective methods of communication. Through the Behavior Rehearsal Technique, family members can be expected to learn, practice and improve their communications skills and learn to communicate more congenially. Second, to increase interaction and acquire more support from the patient's extended family, direct family members can induce their participation by organizing family meetings. Before a social worker initiates a family meeting, he should use a genogram to become aware of the source of support for an Alzheimer patient and his direct family. In addition, he should recognize the adverse effects and causes of stress in a particular family and also obtain active support and attention from the extended family for the direct family members of a patient suffering from Alzheimer's. Third, emotional control is the most important factor that determines the level of family maladaptation. In order to improve emotional control, family members can use Family Hardiness Strengthening Skills (Maddi & Kobas, 1984 59In order to strengthen the family hardiness, private or group therapy can be considered for the family members. However, because there are fewer distractions, one-on-one private therapy is recommended and viewed to be far more effective for family members who are currently having difficulty adjusting their lives to those of Alzheimer's patients. Family Hardiness Strengthening Therapy uses the following techniques: First, the "Focalization" Approach gives clients an opportunity to recall feelings of neglect and personal longings during childhood as well as encourages them to recognize and express their true feelings and thoughts. Second, the "Situation Reenactment" Approach permits clients to first imagine a stressful situation that either becomes worse or better. Afterwards, the client is told to imagine what must change or happen for that situation to occur and what the client must do so that these situations do occur. The last stage of this approach is actually putting these trial situations into practice. Third, the "Compensational Self-Improvement" Approach can be used in stressful situations where little or no improvement at all can be expected from the patient. Family members should be encouraged to accept the present situation and use their energy in a constructive way to improve themselves in other areas. Fourth, the "Paradoxical Intention" Approach will allow clients suffering from severe depression and insomnia to regain self-control by allowing symptoms to become more severe -- in other words, making it worse to get rid of it. Of the above mentioned approaches, the "Situation Reenactment," "Compensational Self-Improvement," and "Paradoxical Intention" approaches are considered to be the most effective ways to obtain and improve the family's emotional control. Last of all, the following group of people require special and foremost attention because they experienced a relatively difficult time adjusting to the patient: people with low-income and little or no education, people with no background knowledge regarding Alzheimer's disease, people with a pessimistic view of the patient's recovery and people who lack an intimate and close relationship with the patient. These groups of people can participate in the Family Education Program to gain a better understanding of the patients and Alzheimer's disease. These programs will allow family members to grasp and become more tolerant of the patients' pathological behavior as well as prevent maladjustments that could worsen the relationship between patient and family.
고교학점제 운영을 위한 홈베이스의 적정규모에 관한 연구 : 충청남도 소재 기존 고등학교를 중심으로
The high school credit system is a system in which students select subjects according to their career path and aptitude based on basic knowledge and basic academic ability and graduate by earning and accumulating credits for subjects that meet the completion standards. Students are required to change into proactive beings who choose the classes they need, and schools need changes in space for efficient learning. It is important to highlight the significance of public spaces to ensure the stable operation and successful implementation of the high school credit system. Since there are budget limitations in regard to upgrading all school facilities, the selection of specific areas to concentrate on is vital in transforming these public spaces into accessible and diverse environments. Schools built on the existing subject-classroom system projects should be able to use the Homebase not only as a place for storing lockers but also as a versatile space for rest and small group activities. The Homebase of the subject-classroom system recommends securing 0.7 m2 per person. However, in reality, allocating only 0.3 to 0.4m2 per person for use as storage space for lockers is planned. Cases were compared and reviewed to understand both the storage space needed and the other space required for learning. The appropriate size of the Homebase was analyzed by conducting preliminary planning for the spatial restructuring of a high school credit system in Chungcheongnam-do. An area equivalent to one class is required for every three classes to accommodate storage lockers and provide a space for resting or learning between classes, which translates to approximately 0.9 m2 of space per person. The study began to investigate what kind of space existing schools should adapt to implement the changed curriculum. The conclusions drawn from changes in the curriculum, the limited budget, and the required space were implemented in the public area known as the Homebase, where the necessary dimensions for accommodating various activities were analyzed. A few points need to be added to the case analysis, but based on the previous analysis, it is expected that research on the spatial change of existing schools and the utilization of Homebases will continue even after the high school credit system is implemented. 고교학점제 운영을 위한 홈베이스의 적정규모에 관한 연구: 충청남도 소재 기존 고등학교를 중심으로 논문 제출자 조 영 선 지 도 교 수 박 상 현 고교학점제는 학생맞춤형 교육으로 교실의 사용빈도를 높이고, 미래사회에 필요한 역량양성 및 학생 개개인의 다양성을 지원하는 교육제도이다. 학생은 주어진 교육과정을 수동적으로 따라가는 존재에서 필요한 수업을 스스로 선택 하고, 진로를 개척해 나가는 자기 주도적인 존재로 변화해야 한다. 이에 고교 학점제를 시행하기 위해서는 학교 공간은 과목 선택권 확대에 따른 효율적인 학습 환경이 필요하며, 학습, 지원, 공용공간의 변화를 필요로 한다.1) 고교학점제는 급변하는 미래사회에 대응하기 위한 교육과정으로, 자기 주도 적이며 창의적인 역량을 키워내기 위함이다. 2025년 고교학점제 전면 시행을 앞두고 변화하는 교육과정에 맞춰 학교 공간조성의 필요성이 대두되고 있다. 일부 학교는 시범 운영을 하고 있으며, 다수의 학교들은 고교학점제의 운영을 위해 기존 공간의 재구조화를 진행하고 있다. 그럼에도 여전히 고교학점제를 시행하기에 적합한 공간으로 변화가 이루어지지 않은 학교들이 존재한다. 신설 학교가 아닌 대다수 기존 학교들은 실의 전환이나 일부 증축으로 재구조화를 진행해야 한다. 본 연구는 고교학점제에 적합한 공간을 제안하는데 목적이 있다. 7차 교육 과정이 수립되고, 2009년 교과교실제에 이르기까지 고등학교의 가장 큰 변화는 홈베이스 도입이라 할 수 있다. 충청남도 소재의 고등학교를 중심으로 고교학 점제 운영에 필요한 홈베이스 적정 규모와 계획을 제안하기 위해 다음과 같이 연구를 진행하고자 한다. 첫째, 문헌 고찰을 통해 고교학점제의 도입과 교육과정의 변화를 이해하고 이를 바탕으로 요구되는 공간의 변화에 대해 예측해본다. 둘째, 교육과정에 따른 학교 공간의 변화에 대해 분석하고, 고교학점제 공간 조성 원리에 대해 이해한다. 고교학점제 공간에서 홈베이스의 역할과 특징을 도출하여 방향을 계획한다. 셋째, 충청남도 소재의 고등학교 공간 재구조화 사전기획 사례를 분석하여 고교학점제 운영을 위한 기존 학교들의 공간 변화를 이해한다. 사물함 보관 외 휴식 및 자기주도학습 공간 등 다목적 공간에 필요한 홈베이스의 적정 규모와 특징을 도출한다. 넷째, 연구 내용을 바탕으로 연구자가 실시설계를 진행하였던 충남 소재의 BB고등학교 고교학점제 공간 재구조화를 제안한다. 앞서 살펴본 연구에 대한 결과를 정리하여 향후 기존 학교들의 고교학점제 공간 재구조화 계획에 있어 홈베이스의 적정한 규모와 공간 구성에 대해 제안하고자 한다. 고교학점제의 학교는 학습 공간의 유연성, 지원 공간의 복합성, 공용 공간의 활용성, 동선의 효율화, 공간·교육과정의 연계를 토대로 재구성되어야 한다. 본 연구에서는 고교학점제에 요구되는 공간의 특징들을 반영하여 홈베이스의 적정 규모와 구성에 대해 제안하였다. 이를 바탕으로 고교학점제 도입에 따른 공간 변화와 홈베이스 활용에 대한 지속적인 연구가 이루어지기를 기대한다. 1) 충청남도 교육청, 반갑다 충남고교학점제, p.11, 2022.
At present, almost country all over the world, whether it is underdeveloped, developing, or developed, cannot avoid a competition of technical innovation, Any economically advanced country has been very ardent for faciliating the development of new techniques that are more up-to-date, while any developing country has made an effort to Introduce new techniques from developed countries, has been busy speeding up its economic development, and has devoted itself to accelerating the development of its own technique on the basis of than Korea has succeeded in industrialization to a considerable extent by making a reasonable combination of alien techniques and capitals introduced till the end of the 1970's with its cheap and plentiful labor, but it has not succeeded very much in elevating the standard of technic and creating its own technic. Foreign capital and techniques introduced with ease comparatively from a short-sighted view-point contributed greatly to enalling small and medium enterprises to grow in quantity, but Korea has not made much progress in accumulating the techniques of its small and medium enterprises, because some of techniques mentioned above have included stereo-typed ones in economic terms, old-fashioned ones, and ones that advanced countries intended to export abroad considering their wage level or public pollution, and in addition the policies of such introductions of techniques have been carried out mostly for the sake of mammoth enterprises Poor development of techniques like this on the part of small and medium enterprises have made enterprises passive in attempting to achieve technical innovation in an aggressive and daring attitude in addition to making it hard for gigantic enterprises to put new techniques into practice, because the growth of enterprises, which are to support and promote the advancement of techniques at the back of immence enterprises, has been retarded. If businessmen should fall into the habit of the easy going method of administrations it is difficult for you to expect the development of revolutionary techniques, however promising it may be make use of techniques under development in manufacturing goods, and however optimistic it may be to sell commidities It may be safely said that one of the most important factors i n propelling technical innovation is for enterprises to make a decision to challenge the uncertainty of the future, running a risk in order to cope with and adapt themselves to their ever-changing envisrnmental situations Recently the role of small and medium industries which constitute important bases of national economy has been deeply recognized, and the fact that enhancement o f the techniques of grandiose enterprises can't be expected without piling up techniques supported by small and medium industries has proved real through chronic recession these days, and accordingly the development of techniques of small and medium industries has been realized not only as a cornerstone for their existence and advancement but also as a foundation of the balanced development of national economy by supporting and assisting the technical innovation of grand enterprises
티저(Teaser)광고에서 알레고리(Allegory)의 효과 및 가능성에 관한 연구
광고인이 광고 한편을 제작하는 것을 산고의 고통에 비유하기도 한다. 하지만, 우리는 하루 평균 1,500편의 광고에 노출되며, 평균 76편의 광고를 지각하며 0.8%인 12편 정도만을 기억한다고 한다. 본 연구는 다른 광고와의 차별성 및 주목성을 두어 광고목적을 최대한 전달할 수 있고, 광고효과를 극대화시킬 수 있는 광고기법 중 하나인 티저(teaser)광고에 주목하였다. 티저광고는 처음에는 회사명과 상품명을 밝히지 않고 약간의 단서만 제시하고 끊임없이 수용자의 궁금증을 일으키게 하고, 그 내용을 점진적으로 드러내어 일정시간이 지난 후 한 순간에 베일을 벗기는 광고이다. 수용자에게 친밀감을 주고, 유머러스하게 접근하는 알레고리(allegory)를 티저광고에 접목시켰다. 하나의 단편을 통해 복잡하고 커다란 문맥을 상기시키는 힘이 있는 알레고리는, 수용자에게 새로운 정보를 주는 동시에 다의성을 포함한 내러티브 구조를 가지고 있다. 알레고리는, 의미의 전이에 따른 수사법이라는 점에서 은유와 환유의 속성을 공유하며 공시적인 은유보다는 통시적인 환유에 가깝다고 할 수 있다. 2000~2012년 6월까지 인쇄광고(조선일보, 중앙일보)와 명광주의「한국광고표현모델」, 광고정보센터(adic.co.kr)에서 티저광고를 발췌했다. 발췌한 티저광고 중 4편을 선정하여 알레고리를 적용한 티저광고를 직접 제작하였다. 알레고리를 적용한 4편의 티저광고와 4편의 미적용 티저광고를 1차로 보여준 후 하루의 시간차이를 두었다. 2차로 알레고리를 활용한 2차 광고 4편과 미적용 2차 광고 4편을 보여준 후 설문조사 하였다. 성별, 연령별로 주목성, 독창성, 인지도, 이해도, 구입의향 등의 광고효과를 검증하였다. 조사대상은 20대 이상 성인을 대상으로 SPSS 16 통계프로그램을 이용한 신뢰도분석, 독립표본 t검증, 이원변량분석(Two way Anova)을 실시하였다. 검증결과 티저광고에서 알레고리를 활용하는 것이 조금 더 효과적인 것으로 나타났다. 분석과정에서 유추된 연령에 대한 특성으로 알레고리 활용에 상관없이 티저광고에 대한 전체적인 광고효과는 저연령층(20대~30대)보다 고연령층(40대~50대 이상)에서 평균적으로 높게 나타났다. 알레고리를 활용한 광고에 대한 효과는 저연령층에서 변화폭이 크게 나타나 알레고리를 활용하는 광고는 저연령층을 대상으로 시행했을 때 효과적인 것으로 나타났다. 성별에 대한 특성으로는 여성이 전체적으로 광고효과에 대한 평가가 높았다. 남성의 경우 알레고리를 활용한 광고에서 여성보다 변화폭이 크게 나타나 알레고리를 활용하는 광고는 여성보다 남성을 대상으로 실시하는 것이 효과적인 것으로 나타났다. To make an advertisement for an advertiser is compared to labor pains. However, we are normally exposed of 1,500 ads per day, and aware of average 76 ads, and remember of 12 ads, which is 0.8% out of total exposure per day. This study is focused on a teaser advertisement which is one of the advertisement technique for maximizing the effect, and the communication of ad with distinctiveness and attention. A teaser advertisement is an advertisement without revealing the name of company and the brand, showing a bit of clue to draw attention of the people and creating curiosity, and unveiling one-moment in a certain period of time after gradually revealing. Allegory which is approaching humorously, presenting an intimacy to customers, is combined with a teaser advertisement. Allegory, which has the power of reminding a complicated and large context as one fragment, has narrative structure including polysemy, and providing the new information to customers. Allegory may be closed to diachronic metonymy rather than formal metaphor in a certain point of rhetoric to transfer meanings, sharing the attribute of both metaphor and metonymy. Teaser advertisements were extracted from 'adic.co.kr' and 'Korea advertisement expression model', 'Printing ad'(Chosun daily newspaper and Joongang daily newspaper) from year 2000 to 2012 June. Selected 4 teaser ads out of extraction, and produced teaser advertisements with the application of allegory. Firstly showing 4 teaser ads with an allegory, and 4 teaser ads without an allegory. One day later, survey was conducted after showing another 4 teaser ads with an allegory and 4 teaser ads without an allegory The effectiveness of advertisement was verified by gender, attention by age group, creativity, awareness, understandability, intention of buying etc. With an age of over 20, reliability analysis by using SPSS 16 statistics program, Independent samples t-verification, Two way Anova was conducted. As a result of verification, allegory in teaser ads was a bit more effective. In the analysis process, characteristics by inferred ages, regardless of using allegory, the total advertisement effectiveness for teaser ads was generally higher in an old aged group (ages over 40~50) than young aged group (ages over 20~30). The effectiveness for teaser ads with using allegory became higher among the young aged group, and it is obvious that teaser ads with using allegory was effective for the young aged group. Characteristics by gender, female group was generally higher in advertisement effectiveness than male. In case of male, teaser ads with using allegory became higher than female in the range of fluctuation, it showed that teaser ads with using allegory is more effective to male than female.