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식육 중 항균물질(플루오르퀴놀론계) 동시 다성분분석법 개선 연구
박동엽 ( Dong Yeob Park ),황보원 ( Bo Won Hwang ),조성숙 ( Sung Suk Cho ),최찬영 ( Chan Young Choi ),조상래 ( Sang Lae Cho ),박애라 ( Ae Ra Park ),정은희 ( Eun Hee Jung ),변유성 ( You Sung Byun ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.2
A direct, accurate and sensitive chromatographic analytical method for quantitative determination of four fluoroquinolones(norfloxacin, cirprofloxacin, danofloxacin and enrofloxacin) in chicken, pork and beef edible muscle is proposed in the present study. The developed method was successfully applied to the determination of enrofloxacin, as the main component of commercially available veterinary drugs. The samples were homogenized and the antimicrobials were added, then they were extracted twice with dichloromethane. Fluoroquinolone antibiotics were separated on an agilent 250×4㎜, C18, 5㎛, analytical column, at 25℃. The mobile phase consisted of a mixture of DW:acetonitrile:triethylamine(80:19:1%, v/v, pH 3.0) leading to retention times less than 14min. at a flow rate 0.5㎖/min. These fluoroquinolones were detected by liquid chromatography with fluorescence at 290㎚ excitation and 465㎚ emission. The limits of quantification in each edible muscle(chicken, pork, and beef) were 0.32-6.54ng/g. Using 0.5g of each sample, average recovery rates at fortification levels of 0.05, 0.1 and 0.2㎍/㎖ ranged 70.14-71.71% for NFX, 71.87-73.89% for CFX, 82.16-92.35% for DFX, and 90.13-98.12 for EFX. This is a simple and economic method to quantify the presence of NFX, CFX, EFX and DFX in edible muscle of animal origin.