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Intrinsically disordered fold of a PIAS1–binding domain of CP2b
조구성,Hae-Ri Jo,김철근,김찬길,원형식 한국자기공명학회 2014 Journal of the Korean Magnetic Resonance Society Vol.18 No.1
The transcription factor CP2 regulates various biological systems at diverse tissues and cells. However, none of the four CP2 isoforms has been solved in structure yet. In particular, two different regions of the CP2b isoform have been characterized to interact with the PIAS1 in nucleus to regulate the a-globin gene expression. Among them, in this study, the region encompassing residues 251-309 of CP2b was prepared as a recombinant protein and its solution structure was characterized by NMR spectroscopy. The results indicated that the CP2b(251-309) fold belongs to typical IDRs (intrinsically disordered regions), likely to facilitate promiscuous interactions with various target proteins. Unfortunately, however, its interaction with the N-terminal domain of PIAS1 (residues 1-70), which has been identified as one of the CP2b-binding sites, was not observed in the NMR-based titration experiments. Therefore, it could be postulated that the 251-309 region of CP2b would not contact with the PIAS1(1-70), but alternatively interact with another CP2b-binding region that encompasses residues 400-651 of PIAS1.
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Backbone NMR Assignments of a Putative p53-binding Domain of the Mitochondrial Hsp40, Tid1
조구성,심대원,김은희,강동훈,마유빈,김지훈,원형식 한국자기공명학회 2018 Journal of the Korean Magnetic Resonance Society Vol.22 No.3
Human Tid1, belonging to the family of the Hsp40/DnaJ, functions as a co-chaperone of cytosolic and mitochondrial Hsp70 proteins. In addition, the conserved J-domain and G/F-rich region of Tid1 has been suggested to interact with the p53 tumor suppressor protein, to translocate it to the mitochondria. Here, backbone NMR assignments were achieved for the putative p53-binding domain of Tid1. The obtained chemical shift information identified five α-helices including four helices characteristic of J-domain, which are connected to a short α-helix in the G/F-rich region via a flexible loop region. We expect that this structural information would contribute to our progressing studies to elucidate atomic structure and molecular interaction of the domain with p53.
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움직임 벡터의 활동성과 방향성을 고려한 트랜스 부호화기의 비트율 제어
구성조,장해원,황찬식 한국통신학회 2003 韓國通信學會論文誌 Vol.28 No.12c
다양한 유무선 네트워크 환경상에서 실시간으로 보다 안정적이고 신뢰성 있는 주문형 비디오 및 실시간 멀티미디어 서비스론 사장자가 제공받기 위해서는 비디오 비트 스트림의 비트율을 다양한 채널의 대역폭에 맞추는 트랜스 부호화(transcoding) 과정이 필요하다. 본 논문에서는 트랜스 부호화 과정에서 움직임 벡터 추정시 매크로블록의 활동도와 방향성을 이용하여 계산랑을 줄이면서 화질을 보장하는 방법을 제안한다. 매크로블록의 활동도와 방향성의 임계치는 복호화 과정에서 추출한 중간정보 중에서 DCT 계수를 사용하여 비디오마다 적합하게 적용이 가능하다. 그리고 제안된 비디오 트랜스 부호화 알고리즘을 이용하여 H.263 환경에서 영상의 비트율 변환을 통한 실험으로 적절한 수준의 하질 유지와 계산량의 감소를 확인 할 수 있었다. The transcoding is needed to modify the bit rate of the video bitstream in accordance with the bandwidth of a variety of channels in order to provide users with a realtime multimedia service and more stable and credible VOD(Video On Demand) service on various wire/wireless network. This paper suggests how to guarantee the video quality in the transcoding process, taking the activity and direction of the macroblock into consideration, thereby reducing the calculation. The threshold of the macroblock's activity and direction is applicable to the different videos, with the DCT coefficients from the intermediate data in the decoding process. Moreover, the suggested video transcoding algorism enables us to maintain the proper video quality and reduce the calculation through the video bit rate change experiment in H.263 environment.
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Sulfonated Polysulfone막의 제조 및 투과특성
김윤조,전종영,구성회,탁태문 한국막학회 1996 멤브레인 Vol.6 No.1
본 연구는 소수성 물질인 PSf의 친수화 및 친수화된 SPSf의 분리막을 제조하여 이의 투과특성과 fouling억제에 대한 친수화의 효과에 관하여 조사하였다. PSf의 친수화는 CSA와 황산을 이용하였으며, 이온교환량은 친수화제의 농도, 반응시간, 반응온도가 증가할수록 증가하는 경향을 보였다. SPSf의 분리막은 이온교환량이 증가할수록, 투과유속은 감소하고 용질배재율은 증가하였다. 막의 fouling억제 정도의 척도인 MFI를 측정한 결과 친수화된 SPSf의 분리막은 PSf의 분리막의 1/4정도의 값을 나타내었다. This study is undertaken to investigate the major factor which causes a fouling phenomenon in membrane separation system and effect of hydrophilic treatment on fouling repression of hydrophobic polymer (polysulfone, PSf). Chlorosulfonic acid (CSA) and sulfuric acid as a sulfonating agents are used to produce sulfonated PSf (SPSf). SPSf membrane is prepared under several conditions to investigate it's separation process. The extent of fouling-repression is measured by membrane filtration index (MFI) value. With increasing the ion exchange capacity (I.E.C), the flux of SPSf membrane is decreased but the rejection is increased and the flux is less reduced. According to the MFI value, the SPSf membrane has a fouling-retardant effect.
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