Evaluation of leaf morphology for distinguishing Prunus (Rosaceae) from Jeju, Korea

김찬수,문명옥,정은주,변광옥,Kim, Chan-Soo,Moon, Myung-Ok,Cheong, Eun Ju,Byun, Gwang Ok The Korean Society of Plant Taxonomists 2005 식물 분류학회지 Vol.35 No.2

본 연구는 제주도산 벚나무속 식물을 대상으로 잎 형질의 분류학적 중요성을 검토하기 위하여 수행하였다 양적 형질은 엽신의 길이, 엽병의 길이, 엽폭, 엽맥 수, 분지각과 엽저각 등을 조사하였다. 질적 형질은 모용분포와 밀선의 위치 잎의 형태 등을 조사하였다. 총 25개의 OTU에 대해 UPGMA에 의한 표현형적 분석을 실시하였다. 그 결과 subgenus Prununophora에 속하는 매실나무가 subgenus Padus에 속하는 귀릉나무와 섬개벚나무와 같은 유집군을 형성한 점과 subgenus Cerasus에 속하는 벚나무, P. speciosa, 산벚나무, 사옥와 올벚나무, 왕벚나무가 별개의 유집군을 형성한 점 등 일부 분류체계와 다른 양상을 보였다 그러나 subgenus Padus에 속하는 귀릉나무와 섬개벚나무, subgenus Microcerasus에 속하는 이스라지, subgenus Amygdalus에 속하는 복사나무가 각각의 유집군을 형성하는 등 분류체계와 유사한 양상이 강하게 나타났다. 결국, 잎 형질, 그 중에서도 엽신의 길이와 폭의 비, 엽신의 길이와 엽병 길이의 비, 엽맥 수, 잎 기부에서 밀선과의 거리, 주맥과 잎 기부의 좌 우각 등과 모용의 분포 등이 제주도산 벚나무속의 식별형질이 될 수 있을 것으로 판단되었다. Leaf morphology was examined for Prunus species from Jeju Island. Analyzed were the leaf blade length, petiole length, width of blade, number of veins and angle of the base to the mid-vein as quantitative characters and the distribution of trichome, position of glandsand leaf features qualitative characters. A total of 25 OTU were phenetically analyzed by UPGMA. The resuling phenograms slightly differ from the currently recognized taxonomic system in two points. Prunus mume was clustered with P. padus and P. buergeriana. Prunu spendula and P. yedoensis were separated from the cluster of P. jaamasakura, P. speciosa, P. sargentii and P. jamasakura var. quelpaertensis. Except for the members of subgenus Cerasus, subgenera Padus (P. buergeriana and P. padus), Microcerasus (P. japonica) and Amygdalus were well defined. Some morphological characters of leaves such as the ratio of blade length to width, the length of blade to petiole, number of veins, the distance between the gland and base, the angle of base to mid-vein, and the distribution of trichome were useful as diagnostic features for Prunus from Jeju Island.

정금나무 열매의 형태적 특성과 안토시아닌 함량 변이

송정호 ( Jeong Ho Song ),정은주 ( Eun Ju Cheong ),김혜수 ( Hyeu Soo Kim ),김문섭 ( Moon Sup Kim ),김세현 ( Sea Hyun Kim ) 한국산림과학회 2015 한국산림과학회지 Vol.104 No.2

To develop superior cultivars for edible or medicinal purposes, we investigated morphological characteristics and total anthocynin contents for the fruit of 24 individuals from 4 populations in Korea. ANOVA analysis showed significant differences among populations and among individuals within population in all characteristics. The mean characteristics of the species were 8.2 mm, 7.8 mm, 0.4 g, 11.7 brix., 7.7 ea and 474.4 mg/100 g in the fruit width, fruit length, fruit weight, soluble solids contents, number of fruit per punch and total anthocyanin contents, respectively. Especially, total anthocyanin contents (474.4 mg/100 g) of V. oldhamii showed 1.9 times higher than that of American blueberry (237.3 mg/100 g) using as control. Consequently, the investigation suggests that V. oldhamii fruits can be used to develop the functional cultivars with enhanced health benefits by the selection breeding. Selection effect with outstanding candidate trees including superior 3 individual trees was estimated at 129.6%, 109.5% and 140.5% in fruit weight, number of fruit per punch and total anthocyanin content, respectively.

우리나라 임목육종 연구동향 : 학술지 논문 키워드 분석을 중심으로

어수형(Soo Hyung Eo),이병주(Byeong-Ju Lee),강규석(Kyu-Suk Kang),강준원(Jun-Won Kang),정은주(Eun Ju Cheong),최명석(Myung Suk Choi) 한국육종학회 2020 한국육종학회지 Vol.52 No.S

Studies on forest tree breeding (FTB) in South Korea started in the 1950s and have achieved remarkable outcomes through various techniques and methods such as selective breeding, cross breeding, introduction breeding, and biotechnology. Despite these outstanding achievements, no attempt has been made to objectively and quantitatively evaluate the results of domestic FTB research. Here, we quantitatively analyzed past research trends using keywords of published journal papers and compared the current domestic research level of FTB with the international level. Using various keywords in this field, we searched for research articles published in the Journal of Korean Society of Forest Science, the Korean Journal of Breeding Science, and the journals listed on the Science Citation Index from 1962 to present. The analysis of these studies showed that the research on FTB has grown quantitatively in Korea, and has been conducted on various subjects. Research topics from Korea, in terms of purpose and species, are not significantly different from those of foreign countries. The genus Pinus has been the major subject of FTB studies both domestically and internationally. However, in foreign FTB research, the trees used for research have diversified each year, while they were not diverse in Korea. The FTB research capacity of Korean national institutions, such as the National Institute of Forest Science, continues to dramatically increase, while the research capacity of universities is relatively low. Based on the results of this analysis, it is necessary to establish a concrete and effective policy for future research and development of FTB.

이미지 기반 식물 수형 측정 프로그램 구현

주진호(Jinho Joo),장경주(Gyeong Ju Jang),정은주(Eun Ju Cheong),임현승(Hyeonseung Im) 한국정보과학회 2021 한국정보과학회 학술발표논문집 Vol.2021 No.12

정보처리 및 복합기술 분야 : 5P-12 ; Violet LED광의 형광 영상을 이용한 콩의 가뭄 스트레스 측정 연구

모창연 ( Chang Yeun Mo ),김문성 ( Moon S Kim ),정은주 ( Eun Ju Cheong ),김기영 ( Gi Young Kim ),임종국 ( Jong Guk Lim ),양진영 ( Jin Young Barnaby ) 한국농업기계학회 2015 한국농업기계학회 학술발표논문집 Vol.20 No.1

다양한 통계분석 기법을 이용한 개느삼(Sophora koreensis Nakai)의 기내 증식 최적 조건 구명

정욱한 ( Ukhan Jeong ),이화 ( Hwa Lee ),박상희 ( Sanghee Park ),정은주 ( Eun Ju Cheong ) 한국산림과학회(구 한국임학회) 2021 한국산림과학회지 Vol.110 No.1

개느삼(Sophora koreensis Nakai)은 우리나라 고유종으로 강원도 일부 지역에 자생하는 식물로 유용 기능성 성분이 포함되어 경제성이 높은 생물자원 식물로 평가된다. 따라서 자원 확보를 위한 대량증식이 필요하다. 조직배양을 이용한 번식은 빠르게 대량증식이 가능하다는 점에서 유용하여 개느삼을 영양체를 이용하여 조직배양을 통해 증식법을 개발하고자 하였다. 줄기 생산에 효과적인 호르몬의 종류와 농도에 대한 실험을 수행하고 다양한 통계분석법으로 분석하여 줄기생산에 가장 효과적인 생장조절물질의 적정조건을 구명하고자 하였다. 본 연구에서는 기내 식물체 개느삼을 세 가지 생장조절물질을 혼합 처리 후 5주간 배양하여 얻은 생장결과를 분산분석(ANOVA)과 반응표면분석(Response Surface Method, RSM), 퍼지 군집(Fuzzy Clustering)분석을 수행하였다. 그 결과 반응표면분석은 ANOVA 분석과 퍼지 군집 분석 결과와 상이하고 실제 생장결과에 부합하지 않아 적정조건 구명을 위한 통계분석법으로 적용하기에 적절하지 않은 것으로 나타났다. 반면 ANOVA 분석과 퍼지 군집 분석은 상위 다섯 가지 처리구에 대해서 줄기생산과 줄기길이 생장에 효과적인 것으로 나타나는 유사한 결과를 보여주었다. 다만 퍼지 군집 분석은 각 처리와 결과값의 예측 확률을 제시해주어 ANOVA 분석에서 나타내는 단순 평균값 비교로 농도의 범위를 나타내어 정확한 조합 선택이 어려운 점을 보완할 수 있었다. 퍼지 군집 분석법 결과에 따르면 개느삼 줄기조직은 5 μM 6-Benzylaminopurine(BA)와 2.5 μM Thidiazuron(TDZ)이 혼합된 배지에서 가장 많은 줄기를(11.03±1.52개, 63.33%) 생산하였으며, 줄기길이 생장은 2.5 μM BA, 2.5 μM TDZ, 2.5 μ M Gibberellic acid(GA₃) 조합된 배지에서 가장 긴 것으로(2.18 ± 0.21 cm, 63.33%) 나타나 개느삼 기내배양을 위한 최적 호르몬의 구성인 것으로 나타났다. Sophora koreensis Nakai is an indigenous plant in Koreawith a restricted natural range, part of which is in Gangwon province. The species is known to contain phytochemicals that have beneficial effects on human health, and it is economically important in bioindustry. Because of the limited number of plants in a small range of habitats, the mass-propagation method should be developed for use and conservation. In vitro tissue culture is a reliable method in terms of mass propagation from selected clones of the species. We investigated the optimal conditions of the medium in this process, especially focusing on the concentrations of plant growth regulators(PGRs) in the culture of stem-containing axillary buds. Three statistical methods, i.e., ANOVA, response surface method(RSM), and fuzzy clustering were used to analyze the plant growth, number of shoots induced, and shoot length with various combinations of PGRs. Results from the RSM differed from those of the other two methods; thus, the method was not suitable. ANOVA and fuzzy clustering showed similar results. However, more accurate results were obtained using fuzzy clustering because it provided a probability for each treatment. On the basis of the fuzzy clustering analysis, stem tissue produced the greatest number of shoots(11.03 per explant; 63.33%) on a medium supplemented with 5-μM 6-benzylaminopurine and 2.5-μM thidiazuron(TDZ). Proliferation of shoots(2.18 ± 0.21 cm, 63.33%) was attained on a medium supplemented with 2.5-μM BA, 2.5-μM TDZ, and 2.5-μM gibberellic acid.

사시나무(Populus davidiana Dode) 의 액아를 이용한 기내대량증식

정은주,이재선 한국임학회 1997 한국산림과학회지 Vol.86 No.2

An effective in vitro multiplication method was developed for clones of Populus davidiana Dode. Ten different media were tested for their effect on shoot multiplication. Both MS and LP medium with 0.2㎎/ℓ BAP appeared to be the best for the shoot multiplication with the rate of 9 shoots per explant. There were significant differences among the clones in both multiplication rate and shoot growth. While some clones did not require BAP to promote shoot formation, others did. More than 60% of in vitro shoots rooted on the half-strength GD medium containing 0.2㎎/ℓ IBA.

소나무류 육종에 있어 임의 증폭 다형 디엔에이(RAPD)지표를 이용한 우량 임목의 조기 선발

로버트 티즈데일,이재선,정은주,문홍규,글렌 데일 江源大學校 林科大學 森林科學硏究所 1995 Journal of Forest Science Vol.11 No.-

지표-형질의 상관은 우량 개체 선발과 유전획득량의 증대를 위해 임목 육종에서 해결되어야 할 중요한 과제 중의 하나로 최근 분자유전학적 수준에서의 임의 증폭 다형 디엔에이 (RAPD) 기술의 발달로 이의 해결이 눈 앞에 다가왔다. 호주 퀸즈랜드산림청과 퀸즈랜드대 임목생물공학연구소가 공동 연구하고 있는 슬래쉬소나무, 카리비아소나무 및 그 교잡종에 있어 이 기술을 이용한 수피 두께에 대한 연구 및 육종 계획 전략을 소개한다. 1대 잡종에서 186개의 지표를 포함한 총 길이 1641cM의 16개 연관군의 유전적 지도가 작성되었고, 이 연관군 지도에 수피 두께를 지배하는 6개의 유전자좌가 추정되었다. 또한, 유전적 지표를 이용한 조기 선발을 위해 먼저 중요 형질을 지배하는 유전자들에 대한 종 특성 유전적 지표를 결정하고, 다음 여러가지 대립유전자형에 대한 지표-대립유전자 상관을 구명하는 2단계 전략이 제시되었다. 소나무류는 발아시 양료로 쓰이는 자성배우체는 모수에서 유래하나, 접합자인 배는 양친수로부터 유래하므로 이러한 이질적 유전 조성을 갖인 종자의 발달을 이용한 RAPD 지표와 형질의 상관 연구는 배 단계에서도 우량 개체의 선발을 가능하게 하여 소나무류 육종의 장래를 밝게 하고 있다. Random amplified polymorphic DNA (RAPD) technology, a recent approach in molecular genetics, is much usable to select the elite trees and to maximize the genetic gain in forest tree breeding program, providing a clue to determine the genetic marker-trait correlation. This review intorduces research on bark thickness and breeding strategy in Pinus elliottii, Pinus caribaea and their hybrid by Queensland Forest Service and ForBio Research Pty Ltd, University of Queensland, which employ RAPD technology. Genetic linkage map of F₁hybrids includes 186 RAPD markers and 16 linkgae groups (1641 cM long in total) and 6 quantitative trait loci are located putatively for bark thickness. Following recent research results and experiences in pine breeding programs, the forseeable stages in the application and development are proposed for marker assisted selectin; stage 1 - determination of species specific markers for genes controlling traits of commercial interest, and stage 2 - determination of marker-allele association for specific allelic variants within pure species. As pines inherit their megagametophytes from the seed parent and zygotic embryos from both male and female parents, the determination of marker-trait correlation is possible even in embryo stage, eventually making ways for the early selection of elite individuals.

제주 자생 왕벚나무(Prunus yedoensis Matsumura)의 기내 줄기 증식을 위한 배양조건 구명

김찬수,정은주 한국임학회 2001 한국산림과학회지 Vol.90 No.2

Multiple shoots were induced from in vitro shoot originated from winter bud of P. yedoensis from Jeju. Most explants grow in similar type among the five different media but affected by supplement of sucrose regardless of media. For mass-propagation various concentrations of BAP or GA₃ were treated in the medium respectively. BAP was very effective to produce multiple shoots and 3.5-9.5 shoots were formed on the explant. The shoots induced on the high levels of BAP have short internodes. No shoots were induced on the treatment of GA₃ but roots were induced on it. When GA₃ was supplemented with the medium containing BAP, multiple shoots were produced from the explants. The medium(WPM) containing with 0.5 ㎎/ℓ BAP and 4.0 ㎎/ℓ GA₃ was most effective to produce multiple shoots. When the explants were cultured for 8 weeks, 39.5 shoots were developed in average.

제주 자생 왕벚나무(Prunus yedoensis Matsumura) 동아의 채취시기와 배지의 조건에 따른 기관유도

김찬수,이재선,정은주 한국임학회 2000 한국산림과학회지 Vol.89 No.4

We tried to mass-propagate Prunus yedoensis from Jeju through tissue culture. We investigated the effect of bud collection time, the concentration of NH₄NO₃ in media and plant growth regulators(BAP, GA₃ and IBA) on the differentiation of winter buds. Buds, taken in February, flushed well regardless of various in vitro conditions. Bud flushing rate was significantly different depending on the collection time. BAP appeared to be effective on bud flushing. Sixty percent of buds taken in October flushed on the media containing 3.0 ㎎/ℓ BAR. No buds flushed on the medium supplemented with IBA. Buds, after flushing in BAP media, grew as foliated shoots and showed a rosette of leaves. When GA₃ supplemented to the BAP-containing media as a higher concentration than that of BAP, shoots elongated and developed into normal shoots. The combination of BAP 1.0 ㎎/ℓ and GA₃ 2.0 ㎎/ℓ is most recommendable for shoot elongation.