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국내 야생 조류에서의 뉴캣슬병 바이러스 항체분포율 조사
최강석(Kang-Seuk Choi),전우진(Woo-Jin Jeon),계수정(Soo-Jeong Kye),윤순식(Soon-Seek Yoon),정우석(Wooseog Jeong),김지예(Ji-Ye Kim),권준헌(Jun-Hun Kwon) 韓國家禽學會 2012 韓國家禽學會誌 Vol.39 No.2
Newcastle disease virus (NDV) infects a variety of birds with a wide range of clinical signs from asymptomatic to severe. During a 10-month period in 2011, a total of 1,024 sera from wild birds including 42 species of birds in 8 orders were collected and the seroprevalence of NDV in wild birds was evaluated by hemagglutination inhibition (HI) test. Evidence of NDV infection was observed in 12.6% (129/1,024) of wild birds with a maximum prevalence reported in Mandarin duck (27.8%, 32/115) followed by Mallard duck (20.8%, 57/274), Spot-billed duck (11.9%, 36/303), Pintail (2.9%, 1/34), Blacktailed gull (2.9%, 1/34), White-fronted goose (1.8%, 1/56) and Common teal (1.4%, 1/69). None of the other 35 species of wild birds were antibody-positive for NDV. Mandarin duck, Mallard duck and Spot-billed duck showed high sero-prevalance of 12.2% to 42% during winter season (November to March). Our results indicate that Mandarin duck, Mallard duck and Spot-billed duck might be natural reservoirs for NDV in Korea and the prevalence of NDV infection in wild birds displayed a seasonal pattern with high prevalence of NDV in winter season (November to March).
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Bacteriophage MS2를 이용한 소독제 효력시험 확립에 관한 연구
이채홍 ( Chae Hong Rhee ),김수희 ( Soohee Kim ),한복희 ( Bokhee Han ),김영욱 ( Young-wook Kim ),허문 ( Moon Her ),정우석 ( Wooseog Jeong ) 한국동물위생학회 2021 韓國家畜衛生學會誌 Vol.44 No.3
In virucidal efficacy testing, the chemical inactivation cannot be determined for all viruses due to the difficulties or the inability to culture sufficiently or the risk of exposure to the viruses. Therefore, disinfectants against these viruses could be evaluated by different methods and surrogate viruses are used as alternative. In this study we developed a method for efficacy testing of veterinary disinfectants using one of the candidate surrogate viruses, bacteriophage MS2, as part of the research on the selection of surrogate viruses for efficiency of efficacy testing of veterinary disinfectants. This method is based on the Animal and Plant Quarantine Agency (APQA) guidelines for efficacy testing of veterinary disinfectants. Bacteriophage and disinfectant are reacted in suspension in accordance with the APQA guidelines and then a newly established double agar layer method is applied for the efficacy test. The double agar layer method is summarized as follows: 1) The bottom agar with 1.5% agar is boiled and cooled before poured into petri dishes at volume of 20 mL, and dried under bio-logical safety cabinet. 2) The top agar with 0.7% agar is boiled and kept at 50℃ before E. coli culture was seeded. 3) The serially diluted bacteriophage MS2-disinfectant mixtures 0.05 mL and E. coli host 0.01 mL (OD<sub>600</sub> 0.2~0.3) are mixed with 5 mL of top agar and incubate them at 50℃ for 5 min for reaction. 4) The resulting mixture is poured over top of a bottom agar plate and rocked sufficiently to ensure that the top agar covers the entire surface of the bottom agar. 5) The double agar layer is then placed under biological safety cabinet to allow the agar layer to solidify and subsequently incubated at 37℃ for 24 hr. 6) Following incubation, the plates may be inspected for plaques and record results.
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또아리물달팽이과(Planorbidae)의 분포, 분류 및 흡충류 유미유충 감염 실태
김종환,정우석,조경화,나영언 忠南大學校 環境問題硏究所 1994 環境硏究 Vol.12 No.-
The distribution, taxonomy of Planorbidid sanils and its cercarial emerging status were performed in the rice peddy field, irrigation canal, pond and lakes around Korean main four rivers (Hangang, Kumgang, Yongsangang and Naktonggang). The collected specimens of snails were identified into three genera and three species as Hippeutis cantori, Gyraulus convexiusculus, and Segmentina hemisphaerula on the basis of their shell feature and sexual organs. H. cantori was distributed around four river basins and the most predominant species, on the other side of Yongsangang, no G. corvexusculus was found. S. hemispaerula was collected all over the four river basins except in the middle and lower stream of Yongsangang. Electrophoretic analysis of protein from foot muscle extract revealed no remarkable differences among three Planorbid species, however the phylogenetic relationships of H. cantori was a remote ancestor than that of the G. convexiusculus and S. hemisphaerula by Isozyme analysis. Natural emerging rates of cercaria from the sanils : H. cantori observed 0.75%, G. convexiusculus 1.27% and S. hemisphaerula 0.11%, respectively. Five species of cercariae were classified as Fibricola seoulensis cercaria, Cercaria prosthogonimus, Cercaria misa, Echinostoma macrochis cercaria and one unidentified cercaria from H. cantori, the cercaria of E. macrochis was also found both snail species ; G. convexiusculus and S. hemisphaerula. Some physicochemical factors including water temperature, pH, electric conductivity, BOD, Ca++, Mg++ and pH of bottom sediment soil were determined at the habitats of Planorbid snail, those factors have not produced any notable influences on the inhabit and distribution of Planorbid snails.
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