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전기영동과 산알카리처리 결합방법에 의한 돼지정액의 X 와 Y 정자 분리에 관한 연구
이용빈,서국성,여정수,정용기 ( Y . B . Lee,G . S . Suh,J . S . Yeo,Y . K . Chung ) 한국축산학회 1980 한국축산학회지 Vol.22 No.4
The combination of the acid-alkali method and eletrophoresis for X-and Y-sperm separation of boar semen was performed We used lactic acid and sodium bicarbonate in acid-alkali method, and electrophoresed at 2.5V and 200 μA. The results obtained were summarized as follows : 1. The frequency of appearance of B-body from alkali treatment of whole semen was significantly higher than that from acid treatment. 2. The frequency of appearance of B-body from alkali treatment at the anode electrode was significantly higher compared with that from acid treatment at the same electrode, while that from acid treatment at the cathode was slightly higher than that from alkali treatment at the same electrode. 3. According to time of treatment, Appearance of B-body from alkali treatment at the anode after electrophonesis was decreased, and the result from acid treatment at the same condition was the opposite, while appearance of B-body from alkali treatment at the cathode after electrophoresis was not changed and that from acid treatment at the same condition was increased. 4. Appearance of B-body from alkali treatment at the anode for 5-10minutes showed the most useful result which was higher than the result (51.9%) from electrophoresis alone by 3 %.
단위가축영양 ; 자돈에 있어 주사용 DL -α- tocopherol 과 DL -α- tocopheryl Acetate 의 상대적 효과
정용기(Y . K . Chung),(D . C . Mahan) 한국동물자원과학회(구 한국축산학회) 1996 한국축산학회지 Vol.38 No.2
An experiment was conducted to evaluate the relative efficacy of injectable dl-α-tocopherol or dl-α-tocopheryl acetate on serum and tissue tocopherol concentrations in young pigs. A total of 39 pigs from 4 litters were allotted to groups and intramuscularly irgected at 3 days of age with one of the two vitamin E sources at 200, 400 or 6($gt;D IU. Saline served as a control injection treatment group. A total of 18 pigs were bled and killed at 3-days postinjection and 21 pigs at 7-days postinjection with liver and loin tissue frozen in liquid N for α-tocopherol analysis. Serum and tissue tocopherol concentrations increased linearly (p$lt;.01) by vitamin E dosage level at both 3- and 7-days postinjection. At 3-days postinjection, serum, loin and liver tocopherol concentrations of the dl-α-tocopheryl acetate group were higher than the dl-α-tocopherol group (P$lt;.05). By 7-days postirtjection, serum and liver tocopherol cancentrations were lower in both vitamin E groups than at 3-day postitgection but had sinular α-tocopherol concentrations for both vitamin E sources. Serum and tissue vitamin E increased with dosage. These results demonstrated that the group injected with the esterified dl-α-tocopherol had higher serum and tissue tocopherol concentrations than those injected with free dl-atocopherol. Liver and serum tocopherol concentrations declined more by 7-days postirgection when the dl-α-tocopheryl acetate was injected.